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• Check PTFE tubing is pushed firmly into pump head tubing
• Check PTFE tubing is not crushed or damaged
• Check Flowcell for blockages
• Check waste pipe for blockages
3.3.1.2. Sample being pumped right through Flowcell
• Check air gap and reset to correct value
3.3.1.3. Bubbles in Flowcell
• Check Flowcell connectors are tightened
• Clean Flowcell with detergent solution
3.3.1.4. Inconsistent readings
• Check volume being sipped – try increasing the volume
• Adjust air gap so the end of the sample is closer to the Flowcell inlet - more of
the sample flushes the previous sample out of the Flowcell
3.3.1.5. Wrong volume being sipped
• Check Sipper calibration
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29004779UM AA 10/2011
5061-046 Rev1
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