Performing Ameasurement; Ultrospec 7000/8000/9000 - biochrom ultrospec 7000 User Manual

5. PERFORMING A
MEASUREMENT

5.1. Ultrospec 7000/8000/9000

The Ultrospec 7000/8000/9000 are dual beam UV/visible spectrophotometers
that contain cell holders for both samples (situated at the front of the cell
chamber) and references (situated at the back of the cell chamber). As dual beam
spectrophotometers continually reference against the reference cell holder it is
possible to perform measurements in one of three ways.
To reference against air:
1. Insert a cuvette containing the sample solution in the sample cell holder, ensure
the reference cell holder is empty and close the sample chamber lid.
2. Press take measurement.
3. Repeat until all sample data has been collected. See the section Saving and
Printing for post measurement options.
To reference against a solvent:
1. Insert a cuvette containing the sample solution in the sample cell holder, insert a
cuvette containing the solvent in the reference cell holder and close the sample
chamber lid.
2. Press take measurement.
3. Repeat until all sample data has been collected. See the section Saving and
Printing for post measurement options.
Note: This methodology is used throughout the user manual.
To correct for both solvent and cell effects (simulate matched cuvettes):
1. Insert cuvettes containing the solvent in both the sample and reference cell
holders and close the sample chamber lid.
2. Press take reference.
3. Remove the cuvette containing solvent from the sample cell holder, empty out the
solvent and replace with the sample solution.
4. Insert the cuvette containing the sample solution in the sample cell holder and
close the sample chamber lid.
5. Press take measurement.
6. Repeat steps 4 and 5 until all sample data has been collected. See the section
Saving and Printing for post measurement options.
Note: This methodology ensures the most accurate measurements.
5061-046 Rev1
29004782UM AA 10/2011
UV-Visible
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