biochrom ultrospec 7000 User Manual
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biochrom ultrospec 7000 User Manual

Uv-visible spectrophotometer
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Ultrospec
UV-Visible Spectrophotometers

User Manual

7000/8000/9000
™
Biochrom US
84 October Hill Rd
Holliston, MA
01746-1388
USA
Telephone: 1-508-893-8999
Toll Free: 1-800-272-2775
Fax: 1-508-429-5732
support@hbiosci.com
www.biochromspectros.com

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  • Page 1: User Manual

    Ultrospec 7000/8000/9000 ™ UV-Visible Spectrophotometers User Manual Biochrom US Telephone: 1-508-893-8999 84 October Hill Rd Toll Free: 1-800-272-2775 Holliston, MA Fax: 1-508-429-5732 01746-1388 support@hbiosci.com www.biochromspectros.com...

  • Page 2: Table Of Contents

    1.6. Service, Repair or Return 12.2. Automatic Saving 12.2. Automatic Saving 12.3. Manual Saving 1.6. Service, Repair or Return 1.6. Service, Repair or Return 2. INTRODUCTION TO THE ULTROSPEC 7000/8000/ 1.7. Disposal 12.3. Manual Saving 12.3. Manual Saving 13. SAMPLE MANAGER 12.4. Exporting Data 1.7.
  • Page 3 Page finder Page finder Accessories Appendix 3.3.1.4. Inconsistent readings 3.3.1.5. Wrong volume being sipped 3.3.1.4. Inconsistent readings INSTALLATION 4. INSTALLING THE INTERNAL PRINTER 3.3.1.5. Wrong volume being sipped 2. PASSIVE ACCESSORIES 4. INSTALLING THE INTERNAL PRINTER 2.1. Single Cell Holder 2.

  • Page 4: Health & Safety

    1. HEALTH & SAFETY 1.1 Safety 1.1.1. General Hazards There a number of warning labels and symbols on your instrument. These are there to inform you where a potential danger exists or particular caution is required. Before commencing installation, please take time to familiarize yourself with these symbols and their meaning.

  • Page 5: Unpacking & Installation

    There are no bio-hazardous materials within the unit; however this unit could be used with bio-hazardous samples. Before using the instrument the customer should have in place decontamination procedures designed to protect laboratory workers from occupationally acquired infections. The sample chamber cell holders are removable and may be decontaminated using the appropriate disinfectant for the bio-hazard in question, rinsed with distilled water and then allowed to dry.
  • Page 6 USB B receptacle for connection to PC On / Off switch Fuse holder Mains inlet Connector for external powered accessories USB A plug for USB memory sticks (Ultrospec 7000, 8000 and 9000 stand alone units only) Internal accessory connector 5061-046 Rev1...

  • Page 7: Equipment Operation

    1.3. Equipment Operation 1.3.1. Controls and Indicators Ultrospec USB LED This indicates that the instrument is writing to the USB memory stick. The memory stick must not be removed whilst this LED is on. POWER/STATUS INDICATOR LED The LED indicates the status of the instrument. Orange - during start up and calibration Green - instrument is on and passed start up calibration tests...

  • Page 8: User Maintenance

    • B efore preparing samples and sample reference blanks, you must be familiar with hazards arising from handling the sample materials and where necessary observe local regulatory practice, personnel protection equipment and measures designed to ensure your safety. • P repare the sample blanks (references). A reference is typically the solution that the sample is dissolved in. For Dual beam units the reference may be placed in the reference path or a separate sample reference may be made by first placing the reference in the sample path and performing a reference measurement.
  • Page 9 If the equipment is operated in a manner not specified, then the protection provided by the equipment may be impaired and the instrument warranty withdrawn. 1.4.1. Troubleshooting PROBLEM POSSIBLE CAUSE Start up calibration fails Check sample (and reference) beam is clear before switch on. Possible optical failure, contact service support.

  • Page 10: Customer Support Contacts

    Note: If you experience any problems with your instrument, please refer to section “1.4.1. Troubleshooting”. If you require further assistance, please contact customer support : 1.5. Customer Support Contacts Note: If you experience any problems with your instrument, please refer to section “1.4.1.
  • Page 11 2. INTRODUCTION TO THE Ultrospec 7000/8000/9000 SPECTROPHOTOMETER The Biochrom Ultrospec 7000/8000/9000 are a range of standalone, easy to use, dual beam UV-visible spectrophotometers with high resolution color touch screens. All spectrophotometers offer a comprehensive range of spectrophotometric and life science applications.
  • Page 12 3. USE WITH Datrys PC SOFTWARE When connected to a PC the Ultrospec 7000/8000/9000 spectrophotometers can be controlled using the Datrys PC software package. Operation using Datrys PC software is described in the Datrys user manual or Datrys help file.

  • Page 13: Frequently Used Icons

    4. FREQUENTLY USED ICONS Button Name Function Forward Advances to the next screen in a sequence arrow Back arrow Returns to the previous screen in a sequence Tick Confirms selection/entry. Saves and exits Cross/exit Exit without saving Buttons On The Sample Measurement Screen Take Performs a reference measurement reference...

  • Page 14: Performing Ameasurement

    5. PERFORMING A MEASUREMENT 5.1. Ultrospec 7000/8000/9000 The Ultrospec 7000/8000/9000 are dual beam UV/visible spectrophotometers UV-Visible that contain cell holders for both samples (situated at the front of the cell chamber) and references (situated at the back of the cell chamber). As dual beam spectrophotometers continually reference against the reference cell holder it is possible to perform measurements in one of three ways.

  • Page 15: Lamp Mode

    6. LAMP MODE When creating a method using an Ultrospec 7000/8000 or 9000 spectrophotometer the user will have the option of setting the Lamp Mode to Precision or Pulse. These modes are described below. Precision Precision Mode is used for applications requiring the most accurate measurements.

  • Page 16: Settings

    7. TYPES OF BOXES Biochrom Ultrospec instruments use different kinds of boxes for parameter selection and entry. These include: Alphanumeric Text Entry The alphanumeric text entry box allows the user to enter letters, numbers and symbols by pressing the abc, 123 and λμ! buttons, respectively. It is possible to toggle between upper and lower case letters and through a list of symbols by pressing the abc and λμ! buttons twice.

  • Page 17: Settings

    Administrator or Supervisor privileges. 8.1. Date & Time The Ultrospec 7000/8000/9000 will arrive with the US EST and date set. This can be changed by pressing the Date and Time button. After the desired date and time have been entered, select the tick to save and exit or the cross to exit without saving.

  • Page 18: Data Output

    8.3. Data Output This is the default saving and printing settings that will be used in all application method parameters. 8.4. User Interface Allows the user to set the desired brightness level of the screen, the alphanumeric text entry mode and the duration after which the screensaver will be displayed (if required).

  • Page 19: Instrument Settings

    8.8. Instrument Information Displays the serial number, user-interface (UI) version, build and release dates and instrument control (IC) version. 8.9. Instrument Settings Instrument Settings allows the user to: 1. Collect a new, temporary baseline. This will be stored until the instrument is switched off.

  • Page 20: User Access

    9. USER ACCESS The Ultrospec 7000/8000/9000 have the option to assign users different access rights. These are set via the user access button. Note: User access is only available to users who have administrator privileges. 9.1. Adding a user To add a new user to the instrument, press the ‘Add user’ button. The Ultrospec 7000/8000/9000 can store up to 16 individual users.

  • Page 21: Applications

    Single Wavelength Absorbance, % transmission or concentration measurements at a single, specified wavelength. Wavescan Wavelength scan between two, user defined wavelengths in the range 190 to 1100 nm. The Ultrospec 7000/8000/9000 allows data overlay, post-scan data manipulation and user configurable peak and valley functions. Kinetics Serial and parallel measurements of absorbance versus time to determine rate or end points.
  • Page 22 Examples of concentration measurements include cholesterol, glucose and urea. Measurement Parameters From the main screen of the Ultrospec 7000/8000/9000 select Applications followed by Single Wavelength to display the screen below. Set Mode to Concentration. Wavelength, Bandwidth (Ultrospec 9000 only), Integration Time and Lamp Mode can be set as required.
  • Page 23 (qualitative analysis) and of estimation (quantitative analysis). The observed features arise due to the various electronic transitions that are possible within a molecule. The Ultrospec 7000/8000/9000 offer a range of post- scan data manipulation options including: 1st order derivative, enabling identification of multiple, unresolved peaks;...
  • Page 24 Saving and Printing. Taking A Measurement To take a measurement using dual beam instruments (Ultrospec 7000/8000/9000) insert a cuvette containing the reference solution in the reference cell holder and a cuvette containing the sample in the sample cell holder and press the take measurement button.
  • Page 25 10.4.1. Serial Kinetics Measurements Serial kinetics is the measurement of the absorbance of a single sample over a specified duration at a specified interval. As these instruments are capable of taking up to 1 reading per second serial kinetics measurements can be used for rapid rate reactions.
  • Page 26 Saving and Printing. Taking a Measurement To take a measurement using dual beam instruments (Ultrospec 7000/8000/9000) insert a cuvette containing the reference solution in the reference cell holder and a cuvette containing the sample in the sample cell holder and press the take measurement button.
  • Page 27 Saving & Printing For details of manual saving and printing see the Saving and Printing section. 10.4.2. Parallel Kinetics Measurements Parallel kinetics is the measurement of the absorbance of >1 sample (up to 8) at a set interval over a specified duration. E.g. for 4 samples a measurement would proceed as follows: The instrument will measure samples 1 to 4, pause for the appropriate interval, measure samples 1 to 4, pause and continue in this manner until the full duration has been reached.
  • Page 28 Taking a Measurement To take a measurement using dual beam instruments (Ultrospec 7000/8000/9000), insert a cuvette containing the reference solution in the reference cell holder and a cuvettes containing samples in positions 1 through 8 and press the take measurement button.
  • Page 29 For details of manual saving and printing see the Saving and Printing section. 10.5. Trace Manager – Overlaying & Manipulating Wavescan & Kinetics Files Trace Manager is the application used by the Ultrospec 7000/8000/9000 to overlay and manipulate wavescan and kinetics files. Samples are loaded into Trace Manager as described below:...
  • Page 30 From Within An Application To overlay saved files with a live trace displayed, Trace Manager can be accessed from within the application. This procedure is required for Limited users as they do not have the ability to access Sample Manager on the main screen. Trace Manager can be accessed from the Wavescan and Kinetics measurement screens using the Trace Manager icon on the options menu.
  • Page 31 The construction of a multi-point calibration curve from standards of known concentration to quantify unknown samples is a fundamental use of a spectrophotometer. The Ultrospec 7000/8000/9000 range of instruments has the advantage of being able to store calibration curves with a method. Each calibration curve can be created using up to 9 standards, with each standard measurement being made of up to 3 replicates.
  • Page 32 Calibration can be set to Standards (the user is required to prepare and measure standards) or Manual (the user inputs both the standard concentrations and standard absorbances). Set Standards, Replicates, Curve Fit and Units as required in your application. Set the concentration values for each of the standards using the numeric entry box. Set the outputs required in your method.
  • Page 33 After the standard curve has been collected press the forward arrow to proceed to the sample measurement screen. To take a measurement using dual beam instruments (Ultrospec 7000/8000/9000) insert the cuvette containing the reference solution in the reference cell holder and the cuvette containing the sample in the sample cell holder and press the take measurement button.
  • Page 34 speeds up analyses as only the required wavelengths are measured. Scan on The instrument will perform a full wavelength scan over a range set under the sample measurement parameters screen. With scan on the user can set both the scan speed and the step size.
  • Page 35 The Standard Specification screen is used to declare a list of all of the standard solutions which will be referenced when creating an equation. E.g. the equation for percentage strength at 500 nm compares the absorbance of a sample to the absorbance of a control standard. This is where the control standard would be defined.
  • Page 36 The Equation Viewer screen provides an overview of any equations created, as well as allowing the user to create new or edit existing equations. Data is inputted as described below. Before any data is inputted Name will read ‘Not set’ and will not appear on the results screen. Name Pressing this allows the user to input a unique, alphanumeric name for the equation.
  • Page 37 The first thresholds screen allows the user to input how many thresholds are required for a result. With Thresholds set to 3 the screen will display the table layout shown left. Setting Thresholds to 2 and 1 will reduce the number of values you can input to 2 and 1, respectively.
  • Page 38 Performing A Measurement – No Standards To take a measurement using dual beam instruments (Ultrospec 7000/8000/9000) insert a cuvette containing the reference solution in the reference cell holder and a cuvette containing the sample in the sample cell holder and press the take measurement button.
  • Page 39 11. LIFE SCIENCE APPLICATIONS This contains two sub folders; Nucleic Acids and Protein. The contents of these sub folders are detailed below: Nucleic Acids Utilizes the absorbance measurements at 230, 260 & 280 nm with optional background correction to perform a concentration and purity check for DNA samples.
  • Page 40 Concentration = A260 × Factor Ultrospec 7000/8000/9000 use the default factors 50, 40 and 33 for DNA, RNA and oligonucleotides, respectively. Compensation for dilution and pathlength can also be entered.
  • Page 41 Saving and Printing. Taking A Measurement To take a measurement using dual beam instruments (Ultrospec 7000/8000/9000) insert a cuvette containing the reference solution in the reference cell holder and a cuvette containing the sample in the sample cell holder and press the take measurement button.
  • Page 42 Measurement Parameters Number of Dyes, this can be set to 1 or 2. Dye 1 Name allows the user to select the dye used in the measurement, the Ultrospec 7000/8000/9000 have 19 dyes pre-programmed and the option for user entry using the Custom Dye option. λ Max, Extinction Coefficient and Correction Factor are only editable when using the Custom Dye option.
  • Page 43 Saving and Printing. Taking a Measurement To take a measurement using dual beam instruments (Ultrospec 7000/8000/9000) insert a cuvette containing the reference solution in the reference cell holder and a cuvette containing the sample in the sample cell holder and press the take measurement button.
  • Page 44 c is the Primer concentration of oligonucleotide (pmoles/ml) in the calculated T or the measured concentration in measured T . In the latter case, concentration is obtained from the equation: c = Abs(260 nm) × Calculated factor × pathlength multiplier × 10 000 Calculated factor and MW are defined below.
  • Page 45 Saving and Printing. Taking a Measurement To take a measurement using dual beam instruments (Ultrospec 7000/8000/9000) insert a cuvette containing the reference solution in the reference cell holder and a cuvette containing the sample in the sample cell holder and press the take measurement button.

  • Page 46: Bca, Bradford, Lowry & Biuret Protein Assays

    11.2. Protein Applications The Ultrospec 7000/8000/9000 contains dedicated methods for both colorimetric protein assays and direct UV measurements. 11.2.1. BCA, Bradford, Lowry & Biuret Protein Assays The BCA, Bradford, Lowry and Biuret protein assays are well established spectrophotometric methods for determining the amount of protein in a sample.
  • Page 47 1. Mix 50 parts of Reagent A (a solution containing bicinchoninic acid, sodium carbonate, sodium tartrate and sodium bicarbonate in 0.1 N NaOH, pH 11.25) with 1 part of Reagent B (4% (w/v) CuSO O), preparing sufficient reagent for all the standards and samples. 2 ml of working reagent is required for each sample. 2.
  • Page 48 Set the outputs required in your method. For more information see the section Saving and Printing. To create the standard curve when using replicates press the replicates button in the bottom right corner to take you to the screen shown below. With Replicates off standards can be measured directly as described below.
  • Page 49 Protein concentration (mg/ml) = (1.55 × Abs280) – (0.76 × Abs260) Protein concentration = (Factor 1 × Abs280) - (Factor 2 × Abs260) Ultrospec 7000/8000/9000 use default A260 and A280 factors of 0.76 and 1.55, respectively. These factors can be edited so that the equation can be applied to other proteins (Equation 2).

  • Page 50: Protein Uv

    Saving and Printing. Taking A Measurement To take a measurement using dual beam instruments (Ultrospec 7000/8000/9000) insert the cuvette containing the reference solution in the reference cell holder and the cuvette containing the sample in the sample cell holder and press the take measurement button.

  • Page 51: Protein A280

    11.2.5. Protein A280 Measurement Parameters Select the Mode you require, Ultrospec 7000/8000/9000 have options for Christian Warburg, BSA, IgG, Lysozyme, Molar Extinction, Mass Extinction and E1% (for Molar Extinction the user will also be required to enter the molar extinction coefficient, and molecular weight and atomic units for Mass Extinction). Set Pathlength and Dilution Factor to the required values. Check above to see if background correction is required.

  • Page 52: Saving And Printing

    This section details the saving and printing options offered by all Ultrospec 7000/8000/9000 spectrophotometers. 12.1. Saving Sample Data The Ultrospec 7000/8000/9000 allows users to save sample data in three different formats: 12.1.1. Internal The sample data is saved to the instrument’s internal memory format. See the Sample Manager section for details on saving and recalling data from the internal memory.

  • Page 53: Automatic Saving

    Note: Any sample data saved manually will override the auto save function 12.4. Exporting Data The Ultrospec 7000/8000/9000 allow users to recall saved sample data from the internal memory or a USB memory stick and save this in another format. This is done...

  • Page 54: Sample Manager

    13. SAMPLE MANAGER Sample Manager is the application used by the Ultrospec 7000/8000/9000 for saving and recalling data from both the instrument’s internal memory and the instrument’s USB format. Sample Manager can be accessed from either the main screen using the Sample Manager button (below left) or from within an application using the Sample Manager icon on the options menu (below right).

  • Page 55: Accessing Sample Manager From The Main

    To unlock a file, highlight the appropriate locked data and press the lock icon at the bottom of the screen. Note: As sample data files saved to a USB memory stick must be deleted using a PC it is not possible to lock USB sample data using Sample Manager. To exit from Sample Manager press the ‘Exit’ icon.

  • Page 56: Recalled Files

    13.4. Recalled Files When recalled, sample data files will display the first sample recorded in a measurement. To access all sample data within a recalled file press the Sample box to display a list of all samples contained within the file. Pressing on the desired file will populate the boxes on the sample measurement screen with the saved data.

  • Page 57: Saving Methods

    Methods saved to the instrument’s internal memory are stored in either the Methods or Favorites folders, both of which are accessed via the main screen. The Biochrom Ultrospec are capable of storing up to 90 methods on the instrument’s internal memory.

  • Page 58: Locking Saved Methods

    14.3. Renaming Method Folders Using the Rename Folder icon on the options menu (the left hand icon) it is possible to rename any of the 9 method folders using alphanumeric text entry. 14.4. Locking Saved Methods Using the lock folder icon on the options menu (the center icon) it is possible to add pass code protection to any of the method folders.

  • Page 59: Backing Up Method Folders To Usb

    USB in the Folder box. The USB option will only appear in the list if a USB memory stick is inserted. Methods saved to a USB memory stick will appear in the Ultrospec 7000/8000/9000 Methods folder in the root directory.

  • Page 60: Printing

    15. PRINTING Printing Sample Data The Ultrospec 7000/8000/9000 allows users to print sample data in one of three ways: Note: Only available printers will be shown in the Print to… options box. 15.1. Internal Printer Fitting of the internal printer is described in the Ultrospec 7000/8000/9000 Accessories Manual at the end of this manual.
  • Page 61 16. Cell Changer 16.1. Operation With a cell changer connected, the Accessories folder under Settings will contain the Cell Changer icon. This provides the user with the means to set the default configuration for the cell changer accessory. Prompt User Yes: The Cell Changer parameter screen is displayed as part of the application parameters, allowing the user to set cell changer...
  • Page 62 Taking A Measurement With A Cell Changer When using the cell changer the software will not display the Take Reference button. Ultrospec 7000/8000/9000 As these instruments continually reference against the reference cell holder there is no requirement to perform a reference measurement before collecting sample data.

  • Page 63: Sipper

    17 . Sipper The Sipper and Flowcell accessories for the Ultrospec range of instruments are designed as an aid to speed up sample handling. The system comprises a peristaltic pump head that is controlled by the software to sip a known, calibrated volume of sample and move this into a Flowcell which is in the sample position of the spectrophotometer.
  • Page 64 Sip Mode • Choice of Waste or Return. Waste mode turns the pump head in one direction and the sample goes to waste after measurement. If return is set then pressing the start button a second time will reverse the motor and the sample will be pumped back out of the inlet tube into the sample vessel. Cross contamination between samples will be worse if return mode is used.
  • Page 65 5. Put the sample tube into the sample, press the silver button on the front of the Sipper. System will sip the set volume and then prompt you to remove the sample tubing. 6. System will then pump the set air gap and then wait the set settle time. If auto read is on, the system will automatically run the sample, if it is off then press the orange cuvette icon to run the sample.

  • Page 66: Specifications

    18. TECHNICAL SPECIFICATIONS Parameter Ultrospec 7000 Specification Ultrospec 8000 Specification Ultrospec 9000 specification Configuration Dual Beam Dual Beam Dual Beam Lamp Xenon Tungsten/Deuterium Tungsten/Deuterium Wavelength Range 190 nm to 1100 nm 190 nm to 1100 nm 190 nm to 1100 nm Wavelength +/- 0.5 nm across wavelength...
  • Page 67 19. CHANGING TUNGSTEN AND DEUTERIUM LAMPS Lamps should only be removed after they have cooled completely, attempting to remove lamps before they have cooled may result in injury. 1. Switch off the Ultrospec 8000/9000 and ensure that the instrument is disconnected from the mains.
  • Page 68 Removing & Replacing The Tungsten Lamp To remove the tungsten lamp, pull the metal clip away from the tip of the lamp and carefully pull the lamp to remove. To insert the new lamp, pull the metal clip away from the housing to provide space for the new lamp to be inserted and carefully insert the pins of the lamp into the lamp housing.
  • Page 69 20. TABLE OF ICONS Icon Title Function Back arrow Returns the user to the previous screen. Forward arrow Advances the next screen in a sequence. Displays the relevant options menu, the exact content of the menu will depend upon Open options menu the location.
  • Page 70 Icon Title Function Used in kinetics applications to view data in a specific section. It is possible to add t0 Section through to t7. Methods saved internally can be stored in one of nine method folders (each capable Method folder of storing up to nine individual methods).
  • Page 71 Icon Title Function Displayed on the status bar. This indicates that the instrument will automatically Auto print - USB print all sample data to the USB printer. Deuterium lamp off Displayed on the status bar. This indicates that the Deuterium lamp is off. Deuterium lamp fail Displayed on the status bar.
  • Page 72 Icon Title Function Used in text entry mode to move the cursor backwards (left) and delete any Backspace/delete unwanted characters. Forward space Used in text entry mode to move the cursor forwards (right). Lower case Selects lower case letters when in text entry mode. Symbols Selects symbols when in text entry mode.
  • Page 73 For variable bandwidth instruments (Ultrospec 9000) this gives the option to set the bandwidth to 4, 2, 1 or 0.5 nm for a measurement. For fixed bandwidths instruments (Ultrospec 7000 & 8000) this box will be greyed out. Used in T calculation to set the sequence of bases.
  • Page 74 Numeric entry. This is the upper limit of a wavescan measurement. Note that when using the Ultrospec 7000/8000/9000 the max wavelength must always be greater than the min wavelength by at least the step value. Toggles between the kinetics measurement modes, serial and parallel.
  • Page 75 Function Toggles between 1 and 2. Used in CyDye DNA application only to set the number of dyes used in the measurement. Numeric entry. This option is only used if the counter ion type in the T calculation is set to Other.
  • Page 76 Function Used in User Interface in Settings to set the text entry mode used for alphanumeric text entry. Used in applications that give a concentration result. Units are entered via either alphanumeric entry or from a list of options. Used in User Access when creating a new user. Numeric entry to 3 decimal places, this is used in the CyDye DNA application and is the volume of the probe in µL.
  • Page 77 Ultrospec 7000/8000/9000 ™ Accessories 5061-046 Rev1...
  • Page 78 1. InstallatIon Before installing any accessory in the Ultrospec 7000/8000/9000 you must ensure that the instrument is disconnected from the mains. 5061-046 Rev1...
  • Page 79 2. PASSIVE ACCESSORIES Accessories that do not interact with the instrument’s software are named passive accessories. Their installation is described below: To allow easy access to the thumbscrew at the front of the accessory open the cell chamber lid and lift out the front panel (below left). With the front panel removed the accessory is removed and replaced as outlined below: 1.
  • Page 80 2.1. Single Cell Holder (supplied with unit) The single cell holder is designed to take measurements of 10 mm pathlength cuvettes. All Ultrospec 7000/8000/9000 are supplied with a single cell holder as standard. 2.2. Variable Pathlength Cell Holder 80-7100-05 The variable pathlength cell holder is designed to use 1, 2, 5, 10, 20, 50 & 100 mm pathlength cells.

  • Page 81: General Information

    • The equipment must be used in accordance with the instructions for use provided by Biochrom. • All modifications and repairs to the equipment must be carried out by Biochrom or its authorized agents. • Modifications must not be carried out unless they conform to approved Engineering Service Information issued according to the appropriate Biochrom procedure.
  • Page 82 2.6.2.1. Controller front and rear panel The front and the rear panel have grids to dissipate the heat generated in the unit. Two fans force the air movement. These grids must be free to guarantee the instrument specifications. The front panel features the display with keypad. The rear panel features the mains power connection with mains voltage selector, the USB connector for remote control, the mains switch and the “Water Fill”...
  • Page 83 The plate containing the serial number is located on the rear panel at the base of the unit. Check that this number is identical to the serial number on the delivery note. If not, please inform Biochrom immediately. 2.6.3.2. Checking mains supply and fuses The Controller is factory set to operate at 220 VAC.

  • Page 84: Operating Instructions

    The Controller has no special electrical external connections which would allow relay control etc. Operations are performed either manually or via remote control using the USB connection. Contact Biochrom for further information about the remote control functions. 2.6.4. operating Instructions 2.6.4.1. Manual operation Main page and functions of the buttons...
  • Page 85 2.6.5. Maintenance The Controller requires very little routine maintenance by the user. Any operation that requires the units to be opened must only be performed by Biochrom technicians or authorized personnel. Always unplug from the mains when opening the units.
  • Page 86 The installation of active accessories that use the internal accessory connector is outlined below. Note: The Ultrospec 7000/8000/9000 will be supplied with a single cell holder as standard; this is removed as described above. 1. To insert the accessory, lift the front end, slide into the brackets at the back of the cell chamber (indicated by the two arrows) and tighten the thumb screw by turning clockwise.
  • Page 87 80-2055-13 PTFE tubing with Flowcell connectors The tubing supplied with the Flowcell from Biochrom is 150 cm in length to allow customers flexibility in how they sip from their sample vessels. Unless you require tubing this long for your application take the tubing with the short Flowcell connector attached and cut the tubing to 65 cm in length.
  • Page 88 Sipper outlet – to Sipper inlet – from pump head tubing sample beak • Take the tall Flowcell connector and connect it to the outlet port of the Flowcell. Slide the whole Sipper unit up and feed the end of the tube through the hole in the front panel underneath the pump head, once again from the inside out and then slide the Sipper unit back down in it’s slot.
  • Page 89 • Check PTFE tubing is pushed firmly into pump head tubing • Check PTFE tubing is not crushed or damaged • Check Flowcell for blockages • Check waste pipe for blockages 3.3.1.2. Sample being pumped right through Flowcell • Check air gap and reset to correct value 3.3.1.3. Bubbles in Flowcell • Check Flowcell connectors are tightened • Clean Flowcell with detergent solution 3.3.1.4. Inconsistent readings • Check volume being sipped – try increasing the volume • Adjust air gap so the end of the sample is closer to the Flowcell inlet - more of the sample flushes the previous sample out of the Flowcell 3.3.1.5.
  • Page 90 4. INSTALLING THE INTERNAL PRINTER Ensure that the instrument is unplugged before installing the internal printer. 1. Before the internal printer can be installed the printer blanking panel must be removed. This is accessed from the back of the unit. 2.
  • Page 91 5. Slide the front legs of the printer in place and lower into the correct position. 6. Secure to the instrument by screwing into place. Reconnect the instrument to the mains and switch on. Once installed the internal printer will appear in the list 5061-046 Rev1 29004779UM AA 10/2011...

This manual is also suitable for:

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