biochrom ultrospec 7000 User Manual page 65

5. Put the sample tube into the sample, press the silver button on the front of
the Sipper. System will sip the set volume and then prompt you to remove the
sample tubing.
6. System will then pump the set air gap and then wait the set settle time. If auto
read is on, the system will automatically run the sample, if it is off then press
the orange cuvette icon to run the sample. Repeat for subsequent samples.
17 .4. Cleaning/Maintenance/Troubleshooting
To ensure good performance from your Sipper system it is essential that routine
maintenance is carried out.
After batch of samples
• Run the Sipper Wash mode pumping deionized water, for 1 minute then remove
sample tubing and pump air until all liquid is pumped into the waste vessel.
At end of day
• Open up pump head to relax pressure on pump head tubing.
Weekly
• Check pump head tubing for signs of wear – replace if necessary.
• Check all connections for leaks.
• Depending upon types of sample being run it may be advantageous to clean the
Flowcell properly (e.g. if "sticky" protein solutions are being run). Unscrew the 2
connectors, take the Flowcell out of the instrument, fill with a detergent solution
and leave to soak overnight. Empty and then flush with deionized water for 1
minute using the wash mode.
Problems with Sipper systems are generally caused by wrongly set parameters or
poor maintenance. Typical faults and their cures include:
Sample not being sipped
• Check pump head closed.
• Check pump head tubing for damage.
• Check Flowcell is connected and connectors are tightened.
• Check PTFE tubing is pushed firmly into pump head tubing.
• Check PTFE tubing is not crushed or damaged.
• Check Flowcell for blockages.
• Check waste pipe for blockages.
Sample being pumped right through Flowcell
• Check air gap and reset to correct value.
Bubbles in Flowcell
• Check Flowcell connectors are tightened.
• Clean Flowcell with detergent solution.
Inconsistent readings
• Check volume being sipped – try increasing the volume.
• Adjust air gap so the end of the sample is closer to the Flowcell inlet - more of the
sample flushes the previous sample out of the Flowcell.
Wrong volume being sipped
• Check Sipper calibration.
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