biochrom ultrospec 7000 User Manual page 48

Set the outputs required in your method. For more information see the section
Saving and Printing.
To create the standard curve when using replicates press the replicates button in
the bottom right corner to take you to the screen shown below. With Replicates off
standards can be measured directly as described below.
Note: After all replicates have been taken for a standard pressing the replicates
icon takes the user to the next standard that was specified in the method.
To create a standard curve using dual beam instruments (Ultrospec
7000/8000/9000) insert the cuvette containing the reference solution in the
reference cell holder and the cuvette containing the first standard/replicate in
the sample cell holder and press the take measurement button. The reference
measurement will be automatically subtracted from the standard measurement.
Continue recording all standards/replicates until the standard curve has been
completed. After the standard curve has been collected press the forward arrow to
proceed to the sample measurement screen.
To ignore any outlying standard measurements press the tick next in the
appropriate row to toggle it to a cross. Any ignored measurement will be
automatically removed from the standard curve. These can be reinstated by
pressing the cross.
Note: Pressing the save method icon before any standards have been measured
will save the method parameters only. Recalling a method containing method
parameters only will require the user to construct a standard curve before
measuring samples.
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