biochrom ultrospec 7000 User Manual page 31

With the required files loaded into Trace Manager press the appropriate Trace
Output box to display the manipulation options.
Wavescan post scan manipulations are:
Sample Data
1st – 4th Derivative
Smoothed
Enhanced
Kinetics post scan manipulations are:
Sample Data
Low
Medium
High
After the manipulations and display options have been set as required press
the forward arrow in the right corner to display the recalled data on the sample
measurement screen.
Note: For wavescan and serial kinetic measurements with overlays set to >1
and parallel kinetic measurements, data will be automatically saved into the
instrument's internal memory and displayed in Trace Manager. To ensure the
optimum performance of the instrument it is recommended that unwanted files
are deleted from the internal memory at regular intervals (see Sample Manager –
deleting files from the internal memory).
10.6. Standard Curve
The construction of a multi-point calibration curve from standards of known
concentration to quantify unknown samples is a fundamental use of a
spectrophotometer. The Ultrospec 7000/8000/9000 range of instruments has the
advantage of being able to store calibration curves with a method. Each calibration
curve can be created using up to 9 standards, with each standard measurement
being made of up to 3 replicates.
Creating a Standard Curve
Set Wavelength, Bandwidth (Ultrospec 9000 only), Integration Time and Lamp Mode
as required. The Sample Seed entered under Sample will be the filename of any file
saved automatically.
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29004782UM AA 10/2011
Displays the raw wavescan data (this is the default option).
Displays the derivative data to the desired order.
Uses the Savitzky-Golay algorithm to reduce noise and
smooth the data.
Enhances features, sharpening peaks and valleys.
Displays the raw kinetics data (this is the default option).
Applies a low level of smoothing to the data.
Applies a medium level of smoothing to the data.
Applies a high level of smoothing to the data.
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