biochrom ultrospec 7000 User Manual page 45

Measurement Parameters
Set the required Base Type (DNA/RNA), whether the base is Phosphorylated, the
Primer Concentration (pmoles/ml), Buffer Molarity and Counter Ion. Counter Ion has
options for Na (sodium), K (potassium), TEA (triethylammonium) and Other, allowing
the user to set the required molecular weight (MW) of the counter ion.
Set the Pathlength and Integration Time you require. Base Sequence allows the user
to enter the known base sequence triplets using the buttons A, C, G and T/U. To
improve readability a comma is added after each triplet.
Bandwidth (Ultrospec 9000 only) and Lamp Mode can be set as you wish. The
Sample Seed entered under Sample will the filename of any automatically saved
file.
Set the outputs required in your method. For more information see the section
Saving and Printing.
Taking a Measurement
To take a measurement using dual beam instruments (Ultrospec 7000/8000/9000)
insert a cuvette containing the reference solution in the reference cell holder and
a cuvette containing the sample in the sample cell holder and press the take
measurement button. The reference measurement will be automatically subtracted
from the sample measurement.
Saving & Printing
For details of manual saving and printing see the Saving and Printing section.
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