biochrom ultrospec 7000 User Manual page 73

21. GLOSSARY OF
BOXES
Box
5061-046 Rev1
Function
Toggles between on and off. Used in all applications to set whether sample data is printed
automatically or not.
Toggles between on and off. Used in all applications to set whether sample data is saved
automatically or not.
Can be toggled on and off. Used in nucleic acid and protein measurements to subtract
the absorbance value at 320 nm. This is done to allow for the effects of turbidity, high
absorbance buffer solutions and the use of reduced aperture cells.
Set by numeric entry. Used in CyDye DNA measurements only and enables the user to
specify the wavelength of background correction.
For variable bandwidth instruments (Ultrospec 9000) this gives the option to set the
bandwidth to 4, 2, 1 or 0.5 nm for a measurement. For fixed bandwidths instruments
(Ultrospec 7000 & 8000) this box will be greyed out.
Used in T calculation to set the sequence of bases. The bases A, C, G, T can be added in
m
DNA mode and the bases A, C, G, U can be added in RNA mode. Bases are grouped in threes
to improve readability.
Used in T calculation to toggle between DNA and RNA base types.
m
Used in User Interface in Settings to set the brightness of the screen.
Numeric entry, used in T
m
Buffer molarity = buffer molarity + total molarity of salt (moles / L).
Used in all standard curve applications to set the method used to collect the standard
curve. Options are for Standards or Manual.
Set by numeric entry, used in the CyDye DNA application only. This is the correction factor
applied to the absorbance of the dye at a specified wavelength.
Used in T calculation only this allows the user to add the type of counter ion used. The
m
options are sodium (Na), potassium (K), triethylammonium (TEA) or other. If other is selected
the molecular weight of this counter ion must be added using Other MW.
Used in all standard curve applications this is the curve fit that will be applied to the
standards' absorbance values. Options are Zero Regression, Regression, Interpolation and
Cubic Spline.
Alphanumeric entry for custom dye name, used in CyDye DNA only.
Numeric entry. This is the delay required before a kinetic measurement commences.
Numeric entry. This is used in nucleic acid and protein applications to compensate for the
absorbance of highly concentrated samples.
calculation only.
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