Protein A280 - biochrom ultrospec 7000 User Manual

11.2.5. Protein A280

Measurement Parameters
Select the Mode you require, Ultrospec 7000/8000/9000 have options for Christian
Warburg, BSA, IgG, Lysozyme, Molar Extinction, Mass Extinction and E1% (for Molar
Extinction the user will also be required to enter the molar extinction coefficient,
and molecular weight and atomic units for Mass Extinction).
Set Pathlength and Dilution Factor to the required values. Check above to see if
background correction is required.
Set Units to encompass the expected concentration of your samples. Bandwidth
(Ultrospec 9000 only), Integration Time and Lamp Mode can be set as you wish. The
Sample Seed entered under Sample will the filename of any saved file.
Set the outputs required in your method. For more information see the section
Saving and Printing.
Taking A Measurement
To take a measurement using dual beam instruments (Ultrospec 7000/8000/9000)
insert the cuvette containing the reference solution in the reference cell holder and
the cuvette containing the sample in the sample cell holder and press the take
measurement button. The reference measurement will be automatically subtracted
from the sample measurement.
If Background is set to On the A320 result will be included in the left hand column
and automatically subtracted from the displayed A260, A280 and A260/A280
results.
Saving & Printing
For details of manual saving and printing see the Saving and Printing section.
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