biochrom ultrospec 7000 User Manual page 25

10.4.1. Serial Kinetics Measurements
Serial kinetics is the measurement of the absorbance of a single sample over a
specified duration at a specified interval. As these instruments are capable of taking
up to 1 reading per second serial kinetics measurements can be used for rapid rate
reactions.
Note: Without a cell changer connected, the instrument is only capable of performing
serial kinetics measurements.
Measurement Parameters
Wavelength, Bandwidth (Ultrospec 9000 only), Integration Time and Lamp Mode can
be set as you require. The Sample Seed entered under Sample will be the filename
of any automatically saved file. No. of Samples is described below.
Note: Measurement Mode only appears if a cell changer is connected. If this option
is not displayed the instrument will perform a serial kinetics measurement.
Note: When performing a serial kinetics measurement with >1 sample the
measurement proceeds as follows: Sample 1 will be measured for the full duration
at the specified interval, after this is complete sample 2 will be measured for
the full duration at the specified interval. The measurement will continue in this
manner until all samples have been recorded. When measuring >1 sample, all data
will be overlaid at the end of the measurement and automatically saved to the
instrument's internal memory.
Set the Delay (time before first measurement), Duration (total measurement time,
up to 180 minutes), Interval (duration between readings, from 1 second to duration)
and Integration Time that you require.
Note: The integration time is determined by the interval (i.e. the maximum
integration time is half the interval time).
Mode has options for Delta A, Final A and Slope and is the value that will be
multiplied by the Factor to give the Result on the sample measurement screen.
Units are entered using alphanumeric text entry and will appear on any printed or
exported data. Y min and Y max are what is displayed during the measurement,
the y axis auto-scales upon completion.
5061-046 Rev1
29004782UM AA 10/2011 25
25