Protein Uv - biochrom ultrospec 7000 User Manual

11.2.4. Protein UV

Measurement Parameters
Set Pathlength and Dilution Factor to the required values. Check above to see
if background correction is required. The default values for A260 Factor and
A280 Factor are 0.76 and 1.55, respectively; these can be edited by pressing the
appropriate box. Set Units to encompass the expected concentration of your
samples.
You may advance to the next screen at any time by pressing the forward arrow and
return to the previous screen by pressing the back arrow.
Bandwidth (Ultrospec 9000 only), Integration Time and Lamp Mode can be set as
you wish. The Sample Seed entered under Sample will the filename of any saved
file.
Set the outputs required in your method. For more information see the section
Saving and Printing.
Taking A Measurement
To take a measurement using dual beam instruments (Ultrospec 7000/8000/9000)
insert the cuvette containing the reference solution in the reference cell holder and
the cuvette containing the sample in the sample cell holder and press the take
measurement button. The reference measurement will be automatically subtracted
from the sample measurement.
If Background is set to On the A320 result will be included in the left hand column
and automatically subtracted from the displayed A260, A280 and A260/A280
results.
Saving & Printing
For details of manual saving and printing see the Saving and Printing section.
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