Starting Your Detector; Setting The Chromatographic Conditions - Agilent Technologies 1100 Series Reference Manual

Starting Your Detector

1 Turn on the detector.
2 Turn on the lamp.
3 You are now ready to change the settings of your detector.

Setting the Chromatographic Conditions

1 Set up the system with the following chromatographic conditions and wait until the
Table 4
Mobile phases
Column
Sample
Flow rate
Compressibility A (water)
Compressibility B (Acetonitrile)
Stroke A and B
Stop time
Injection volume
Oven temperature (1100)
FLD Excitations/Emission Wavelength
FLD PMT Gain
FLD Response time
1100 Series FD Reference Manual 35
When the lamp is turned on the first time the instrument performs some internal
checks and a calibration check which takes about 5 minutes.
baseline gets stable.
Chromatographic Conditions
First Steps with the Fluorescence Detector 2
A = water = 35%
B = Acetonitrile = 65%
OSD-Hypersil column, 125 mm x 4 mm i.d. with
5
µm particles
Isocratic standard sample, 1:10 diluted in
methanol
1.5 ml/min
46
115
auto
4 minutes
5 µl
30 °C
EX = 246 nm, EM = 317 nm
PMT = 10
4 seconds