Agilent Technologies 1100 Series User Manual
Nano pump
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- Reference manual (346 pages) ,
- Service manual (288 pages) ,
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Summary of Contents for Agilent Technologies 1100 Series
- Page 1 Agilent 1100 Series Nano Pump User Manual...
- Page 2 Instrument The apparatus is marked with this symbol when the user should refer to the instruction manual in order to prevent risk of harm to the operator and to protect the apparatus against damage. 1100 Series Nano Pump User Manual...
- Page 3 Become an expert In this chapter you will find hints to optimize your nano LC system to achieve best chromatographic results. It also offers diagnosis and build-in tests for maintenance features. Annex A Safety Information 1100 Series Nano Pump User Manual...
- Page 4 1100 Series Nano Pump User Manual...
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Page 5: Table Of Contents
Setup pump parameter screen Priming the pump for best results Manually Priming the Solvent Channels. Purging the Pump Become an expert Hints for Successful Use of the Capillaries Hints for Successful Use of the Micro Vacuum Degasser 1100 Series Nano Pump User Manual... - Page 6 Hints to Optimize the Compressibility Compensation Setting Diagnosis screens Test screens EMF Screen Safety Information Safety Information General Operation Safety Symbols Lithium Batteries Information Danish Information Radio Interference Test and Measurement Sound Emission Manufacturer’s Declaration 1100 Series Nano Pump User Manual...
- Page 7 Solvent Information Solvents Agilent Technologies on Internet Index 1100 Series Nano Pump User Manual...
- Page 8 1100 Series Nano Pump User Manual...
- Page 9 Agilent 1100 Series Nano Pump User Manual Introduction - Around your Nano Pump Introduction to the nano pump Nano Pump Main Overview Pumphead Overview Flow connections Fittings and ferrules Electrical connections In this chapter you will find an introduction to the nano pump and hardware details to identify the parts of the pump and their function.
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Page 10: Introduction - Around Your Nano Pump
Solvent degassing is required for best flow stability and detector stability, especially at the low flow rates required to run nano LC applications. The flow range of the nano pump is between 0.1 µl/min and 1 µl/min. 1100 Series Nano Pump User Manual... -
Page 11: Nano Pump Main Overview
Nano Pump Main Overview Figure 1 Nano pump main overview Pumpdrive A Main Board Damper Power Supply EMPV Outlet Ball Valve A Flow Sensor Pumphead A Pumphead B Solvent Selection Valve Active Inlet Valve B Leak Sensor 1100 Series Nano Pump User Manual... -
Page 12: Pumphead Overview
Screw lock 5042-1303 Pump chamber housing G1311-25200 Seal (pack of 2) 5063-6589 Seal (pack of 2), normal phase applications 0905-1420 Active inlet valve (complete with cartridge) G1312-60010 Replacement cartridge for active inlet valve 5062-8562 1100 Series Nano Pump User Manual... -
Page 13: Flow Connections
170 µm 280 mm G1375-87400 EMPV to Flow Sensor capillary 25 µm 220 mm G1375-87321 Flow Sensor to Sampler capillary 25 µm 350 mm G1375-87322 Flow Sensor to Sampler capillary 25 µm 550 mm G1375-87323 1100 Series Nano Pump User Manual... -
Page 14: Fittings And Ferrules
Fittings and ferrules Fitting+ Swagelock front and back ferrule 5062-2418 (10/pk) Fitting 5063-6593 (10/pk) Lite Touch Ferrule and lock ring 5065-4423 (10/pk) Rheodyne PEEK fitting 5065-4410 (6 fit/2 plug) Fingertight Double winged nut 5065-4422 (10/pk) 1100 Series Nano Pump User Manual... -
Page 15: Electrical Connections
Introduction - Around your Nano Pump Electrical connections Figure 4 Electrical connections Configuration switch GPIB Slot interface board RS232 Analog output Remote CAN-bus Power plug Security lever 1100 Series Nano Pump User Manual... - Page 16 Introduction - Around your Nano Pump 1100 Series Nano Pump User Manual...
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Page 17: Getting Started
Agilent 1100 Series Nano Pump User Manual Getting started Road maps to start an analysis with the nano pump Road map to configure the nano pump Road map to prepare the nano pump for an analysis Road map to start an analysis... -
Page 18: Road Maps To Start An Analysis With The Nano Pump
When the pump is used for the first time or has been turned off for a certain time, it is NO TE recommended to purge each channel at 2500 µl/min for 1 minute at least. Before running the pump in micro mode, run it in normal mode until the system pressure is stable. 1100 Series Nano Pump User Manual... -
Page 19: Road Map To Start An Analysis
18 Set the Set the Micro Mode Solvent Starting an analysis with page 16 Calibration the nano pump page 18 Set the Set the Pressure Limit Gradient Start Timetable Here! page 19 page 18 1100 Series Nano Pump User Manual... -
Page 20: Using The Chemstation To Configure Your Pump
“Hints for Using 0.0 to 99999.9 min. in steps the Fast Composition Change/Reconditioning" on page 30 of 0.1 min. 3 Mixer volume In the standard configuration, no mixer is installed in the nano pump 1100 Series Nano Pump User Manual... -
Page 21: Pump Auxiliary Screen
You may specify the condition as “%” which determines the percentage range where the pump is allowed to start the analysis, or “Always ready”, which starts the analysis independent of the column flow. 1100 Series Nano Pump User Manual... - Page 22 Solvent Compressibility (10 per bar). Isopropanol (2-Propanol) 100 Methanol The compressibility parameter is stored in your method. 1-Propanol For more information see “Hints to Optimize the Toluene Compressibility Compensation Setting" on page 39 Water 1100 Series Nano Pump User Manual...
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Page 23: Setup Pump Parameter Screen
Getting started Setup pump parameter screen To get to this screen, select: ➜ Instrument Setup pump Figure 7 Setup pump parameter screen 1100 Series Nano Pump User Manual... - Page 24 The From 0.00 to 2500 l/min. complete primary flow produced by the two pumping units goes to the column and is not proportioned in the EMPV. 1100 Series Nano Pump User Manual...
- Page 25 You can use Post time to allow 0.0 to 99999 min. your column to equilibrate after changes in solvent OFF (0.0 min.). composition (for example after gradient elution). 1100 Series Nano Pump User Manual...
- Page 26 Select the Display combination box and select Flow/Press or Solvents to display graphical representations of the flow rate/pressure limit or solvent compositions, or Timetable to display the table for editing. In the graphics mode, select Legend to display the graph titles. 1100 Series Nano Pump User Manual...
- Page 27 NOTE: The minimum pressure limit becomes active only maximum pressure setting. when the actual pressure rises above the limit. For the system to create an error, the limits must be exceeded for a few seconds. 1100 Series Nano Pump User Manual...
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Page 28: Priming The Pump For Best Results
4 Disconnect the channel A solvent tube from the A1 port of the pump solvent selection valve. Liquid may drip from the disconnected solvent tube. Make sure to follow appropriate WA RN ING safety precautions. 1100 Series Nano Pump User Manual... -
Page 29: Purging The Pump
When the pump has been turned off for a certain time (for example, overnight), oxygen will NO TE re-diffuse into the channels between the degasser and the pump. It is suggested to purge each channel at 2500 µl/min for 1 minute at the beginning of each day. 1100 Series Nano Pump User Manual... - Page 30 Starts the defined purge sequence. µl/min. 3 Flow Rate Defines the flow rate for the purge in Flow rate: µ from 0.0 to 2500 l/min 4 Purge Time Defines the purge time in minutes 1100 Series Nano Pump User Manual...
- Page 31 To clean the system when using Bidistilled water Best solvent to re-dissolve salts buffers After a solvent change Bidistilled water Best solvent to re-dissolve salts To clean the capillaries Acetone Best solvent to remove impurities from the capillaries 1100 Series Nano Pump User Manual...
- Page 32 Getting started 1100 Series Nano Pump User Manual...
- Page 33 Agilent 1100 Series Nano Pump User Manual Become an expert Hints for Successful Use of the Capillaries Hints for Successful Use of the Micro Vacuum Degasser Hints for Successful Use of the Nano Pump Hints for Successful Use of the Micro Well Plate Sampler...
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Page 34: Become An Expert
Crushed fused silica tubing will distribute small particles throughout the down stream flow path and require complete flushing of all fittings and valves from the point of the damaged tube. 1100 Series Nano Pump User Manual... - Page 35 • Partially blocked capillaries may generate flow oscillations when the sampler is in mainpass mode and the sample is injected directly onto the analytical column. These oscillations are not observed when the trapping mode configuration is used. 1100 Series Nano Pump User Manual...
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Page 36: Hints For Successful Use Of The Micro Vacuum Degasser
• nano pump was turned OFF for a length of time (for example during night) and volatile solvent mixtures are used. For more information see the Reference Manual for the Agilent 1100 series micro vacuum degasser. 1100 Series Nano Pump User Manual... -
Page 37: Hints For Successful Use Of The Nano Pump
• Prevent blocking of solvent inlet filters (never use the pump without solvent inlet filter). Growth of algae must be avoided, either by using acidic (pH=3) media and/or changing solvent every day. 1100 Series Nano Pump User Manual... -
Page 38: Hints For Using The Fast Composition Change/Reconditioning
The Fast Composition Change/Reconditioning function is available only when the nano NO TE pump is operated in the micro mode. How the Function Works Regardless of when it occurs, the Fast Composition Change/ Reconditioning function is always a 2-step process: 1100 Series Nano Pump User Manual... - Page 39 The column is reconditioned for the Column Reconditioning time defined in the user interface. In a sequence, the next injection will begin when Fast Composition Change/Reconditioning is completed. 1100 Series Nano Pump User Manual...
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Page 40: Hints For Successful Use Of The Micro Well Plate Sampler
• Pressure drop with injector valve in bypass (e.g. during drawing sample) should be comparable to mainpass mode, otherwise the seat or the capillary might be blocked. 1100 Series Nano Pump User Manual... -
Page 41: Hints For Successful Use Of The Column And Switching Valve Compartment
The column compartment also houses the 6-port micro switching valve necessary to switch and control the direction of flow of the mobile phases during multi-dimensional separations using two pumps and either two or three columns. 1100 Series Nano Pump User Manual... -
Page 42: Best Practices For Filters
If the filter is in good condition the solvent will freely drip out of the solvent tube (due to hydrostatic pressure). If the solvent filter is partly blocked only very little solvent will drip out of the solvent tube. 1100 Series Nano Pump User Manual... -
Page 43: Inline Filter
Never run the nano pump without an inline filter. NO TE The Nano LC/MS does not require a mixer, since baseline ripples as known from UV detection are not issue for the MS. 1100 Series Nano Pump User Manual... -
Page 44: Hints For Successful Use Of Solvents And Mobile Phase
A,B and a needle wash solvent. The composition would be as follows: • A = 0.1% Formic acid • B = 0.1% Formic acid • Needle wash = 15% Methanol, 84.9 %Water, 0.1 % Formic acid. 1100 Series Nano Pump User Manual... -
Page 45: Hints For Choosing The Primary Flow
(<3 min.). Solvent consumption is highest in this range. Table 2 on page 38 gives approximate primary flow values (in µl/min) as a function of selected primary flow range vs. system pressure: 1100 Series Nano Pump User Manual... - Page 46 Default range Fast gradient range 1190 1580 Actual primary flow values may vary from system to system. If the standard configuration is changed, the primary flow may be higher than the values given in Table 1100 Series Nano Pump User Manual...
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Page 47: Hints To Optimize The Compressibility Compensation Setting
5 Starting with a compressibility setting of 10 × 10 /bar increase the value in steps of 10. Re-zero the integrator as required. The compressibility compensation setting that generates the smallest pressure ripple is the optimum value for your solvent composition. 1100 Series Nano Pump User Manual... - Page 48 Optimize your compressibility settings by using the values for various solvents listed in the following table: Table 3 Solvent Compressibility Solvent (pure) Compressibility (10 /bar) Acetone Acetonitrile Benzene Carbon tetrachloride Chloroform Cyclohexane Ethanol Ethyl acetate Heptane Hexane Isobutanol Isopropanol Methanol 1-Propanol Toluene Water 1100 Series Nano Pump User Manual...
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Page 49: Diagnosis Screens
Diagnosis system screen Diagnosis system screen 1 Nano pump - general information General information relative to the • Product Number pump • Pump Firmware Revision • Manufacturing Date • Serial Number • Pump On Time 1100 Series Nano Pump User Manual... - Page 50 Become an expert To get to this screen, select: ➜ ➜ Diagnosis right mouse-click: Figure 10 Diagnosis pump screen 1100 Series Nano Pump User Manual...
- Page 51 • Date changes logbook 7 Book • Setup pump • Control • Auxiliary • Data curves • Configuration • Purge 8 Thermometer • Pump mainboard temperature history 9 Identify • LED blink on the module 1100 Series Nano Pump User Manual...
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Page 52: Test Screens
• Normal mode pressure test • Leak test For more information about these • Nano pump EMPV test tests see the Service Manual • Nano pump flow sensor accuracy G2226-90100 Chapter 3, calibration Troubleshooting and Test Functions. 1100 Series Nano Pump User Manual... -
Page 53: Emf Screen
You can set a limit for the parameter by entering • Liquimeter (B) the value in the edit field. A value is reset when an entry for the associated part is made in the Maintenance Logbook. 1100 Series Nano Pump User Manual... - Page 54 Become an expert 1100 Series Nano Pump User Manual...
- Page 55 Agilent 1100 Series Nano Pump User Manual Safety Information Safety Information Lithium Batteries Information Danish Information Radio Interference Sound Emission Solvent Information Agilent Technologies on Internet The following general safety precautions must be observed during all phases of operation, service, and repair of this instrument. Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design, manufacture, and intended use of the instrument.
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Page 56: A Safety Information
(normal blow, time delay, and so on) are used for replacement. The use of repaired fuses and the short-circuiting of fuseholders must be avoided. Any adjustment, maintenance, and repair of the opened instrument under voltage is WA RN ING forbidden. 1100 Series Nano Pump User Manual... -
Page 57: Safety Symbols
Indicates a protected conductor terminal. Eye damage may result from directly viewing the light produced by the Xenon flash lamp used in this product. Always turn the xenon flash lamp off before removing it. 1100 Series Nano Pump User Manual... - Page 58 Do not proceed beyond a CAUTION notice until the indicated conditions are fully understood and met. 1100 Series Nano Pump User Manual...
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Page 59: Lithium Batteries Information
Lithiumbatteri - Eksplosionsfare. Ved udskiftning benyttes kun batteri som anbefalt WA RN ING av apparatfabrikanten. Brukt batteri returneres appararleverandoren. Bij dit apparaat zijn batterijen geleverd. Wanneer deze leeg zijn, moet u ze niet weggooien NO TE maar inleveren als KCA. 1100 Series Nano Pump User Manual... -
Page 60: Radio Interference
Safety Information Radio Interference Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations. Test and Measurement If test and measurement equipment is operated with equipment unscreened cables and/or used for measurements on open set-ups, the user has to assure that under operating conditions the radio interference limits are still met within the premises. -
Page 61: Sound Emission
This product has a sound pressure emission (at the operator position) < 70 dB. • Sound Pressure Lp < 70 dB (A) • At Operator Position • Normal Operation • According to ISO 7779:1988/EN 27779/1991 (Type Test) 1100 Series Nano Pump User Manual... -
Page 62: Solvent Information
• Solutions containing strong complexing agents (for example, EDTA, ethylene diamine tetra-acetic acid). • Mixtures of carbon tetrachloride with 2-propanol or THF. • Avoid the use of alkaline solutions (pH > 8.5) which can attack the fuse silica from the capillaries. 1100 Series Nano Pump User Manual... -
Page 63: Agilent Technologies On Internet
For the latest information on products and services visit our worldwide web site on the Internet at: http://www.agilent.com Select “Products” - “Chemical Analysis” It will provide also the latest firmware of the Agilent 1100 series modules for download. 1100 Series Nano Pump User Manual... - Page 64 Safety Information 1100 Series Nano Pump User Manual...
- Page 65 29, fast composition change, cleaning, optimize your system, fast composition/reconditioning, solvent information, 26, overview, nano pump, fast reconditioning, solvents, 18, filter, sound emission, filter volume, stoptime, 1100 Series Nano Pump User Manual...
- Page 66 Index switching valve compartment, timetable, tubing, vacuum degasser, 1100 Series Nano Pump User Manual...
- Page 68 Agilent Technologies In This Book ' #(& ' #(& G2226-90000...