Agilent Technologies 1100 Series Reference Manual page 54

2 First Steps with the Fluorescence Detector
Stop time
Post time
Injection volume
Oven temperature (1100)
FLD PMT Gain
FLD Response time
Select a Excitation
wavelength in the
low UV
(230...260 nm). This
will cover nearly all
fluorescence in your
sample.
DO NOT select
additional emission
wavelengths (B, C,
D). Doing so will
increase the scan
time and will lower
the performance.
Figure 18
2 Wait until the baseline stabilizes. Complete the run.
3 Load the signal. (In this example just the time range of 13 minutes is
54 1100 Series FD Reference Manual
Detector Settings for Emission Scan
at 14.5 minutes %B=90
at 22.5 minutes %B=95
26 minutes
8 minutes
1 µl
30 °C
PMT = 15
4 seconds