Page 1 Agilent 1100 Series Diode Array and Multiple Wavelength Detector SL User Manual...
Page 2 WA R N I N G contains the complete information about The hardware and/or software described in the Agilent 1100 Series Diode Array and this document are furnished under a license A WARNING notice denotes a Multiple Wavelength Detector SL. It is avail- and may be used or copied only in accor- hazard.
Page 3 Troubleshooting and Diagnostics This chapter gives an overview about the troubleshooting and diagnostic features.s Maintenance and Repair This chapter provides general information on repairing the detector. Maintenance This chapter describes the maintenance of the detector. 1100 Series DAD and MWD User Manual...
Page 4 This chapter provides information on parts for maintenance. Control Module Screens This chapter provides an overview on the control module screens. Annex A Appendix This chapter provides addition information on safety, legal and web. 1100 Series DAD and MWD User Manual...
Page 5: Table Of Contents
TCP/IP parameter configuration Configuration Switches Initialization mode selection Link configuration selection Automatic configuration with Bootp Configuring the Agilent Bootp service program Storing the settings permanently with Bootp Manual Configuration With Telnet With Handheld Controller (G1323B) 1100 Series DAD and MWD User Manual...
Page 6 Two detectors of same type (e.g. G1315C and G1315C) Two detectors of similar type (e.g. G1315C and G1315A/B) How to optimize the Detector Optimizing the Detector Performance Optimization Overview Optimizing for Sensitivity, Selectivity, Linearity and Dispersion Flow Cell Path Length 1100 Series DAD and MWD User Manual...
Page 7 Warnings and Cautions Cleaning the Detector Using the ESD Strap Maintenance Overview of Maintenance Exchanging a Lamp Exchanging a Flow Cell Repairing the Standard or Semi-Micro Flow Cell Repairing the High Pressure Flow Cell 1100 Series DAD and MWD User Manual...
Page 8 Major keys on the Agilent 1100 Control Module Control Module - Unsupported Module Screens available from the Analysis screen Screens available from the System screen Screens available from the Records screen Diagnostics and Tests 1100 Series DAD and MWD User Manual...
Page 9 Appendix General Safety Information The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC) Radio Interference Sound Emission UV-Radiation Solvent Information Declaration of Conformity for HOX2 Filter Agilent Technologies on Internet 1100 Series DAD and MWD User Manual...
Page 10 1100 Series DAD and MWD User Manual...
Page 11 Agilent 1100 Series Diode Array and Multiple Wavelength Detector SL User Manual Site Requirements and Specifications Site Requirements Physical Specifications Performance Specifications This chapter provides information on environmental requirements, physical and performance specifications. Agilent Technologies...
Page 12: Site Requirements And Specifications
It plugs into the power-input socket at the rear of the detector. The male end of each power cord is different and designed to match the wall socket of a particular country or region. 1100 Series DAD and MWD User Manual...
Page 13 (1.0 inches) of space on either side and approximately 8 cm (3.1 inches) in the rear for air circulation and electric connections. If the bench should carry a Agilent 1100 Series system, make sure that the bench is designed to bear the weight of all modules.
Page 14 The G1315C DAD and G1365C MWD are designed to operate in a typical electromagnetic N O T E environment (EN61326-1) where RF transmitters, such as mobile phones, should not be used in close proximity. 1100 Series DAD and MWD User Manual...
Page 15: Physical Specifications
Non-condensing Operating altitude Up to 2000 m (6500 ft) Non-operating altitude Up to 4600 m (14950 ft) For storing the detector Safety standards: IEC, CSA, UL, EN Installation category II, pollution degree 2 1100 Series DAD and MWD User Manual...
Page 16: Performance Specifications
Site Requirements and Specifications Performance Specifications Table 2 Performance Specifications Agilent 1100 Series DAD and MWD Type Specification Comments Detection type 1024-element photodiode array Light source Deuterium and tungsten lamps The UV-lamp is equipped with I.D. tag that holds lamp typical information.
Page 17 Site Requirements and Specifications Table 2 Performance Specifications Agilent 1100 Series DAD and MWD, continued Type Specification Comments Analog outputs Recorder/integrator: 100 mV or 1 V, output range 0.001 – 2 AU, two outputs Communications Controller-area network (CAN), RS-232C, APG...
Page 18 Site Requirements and Specifications 1100 Series DAD and MWD User Manual...
Page 19: Installing The Detector
Agilent 1100 Series Diode Array and Multiple Wavelength Detector SL User Manual Installing the Detector Unpacking the Detector Optimizing the Stack Configuration Installing the Detector Flow Connections to the Detector This chapter provides information on unpacking, checking on completeness, stack considerations and installation of the detector.
Page 20: Unpacking The Detector
Damaged Packaging If the delivery packaging shows signs of external damage, please call your Agilent Technologies sales and service office immediately. Inform your service representative that the detector may have been damaged during shipment. If there are signs of damage, please do not attempt to install the detector.
Page 21 8710-1534 Hex key 4 mm, 15 cm long, T-handle 8710-2392 Hex key 1.5 mm, 10 cm long, straight-handle 8710-2393 ESD wrist strap 9300-1408 * or kit 5062-2418 1/16" Fittings and Ferrules (front/back) 10/PK 1100 Series DAD and MWD User Manual...
Page 22: Optimizing The Stack Configuration
Installing the Detector Optimizing the Stack Configuration If your detector is part of a complete Agilent 1100 Series system, you can ensure optimum performance by installing the following configuration. This configuration optimizes the system flow path, ensuring minimum delay volume.
Page 23 Installing the Detector AC power Remote cable AC power CAN Bus cable AC power CAN Bus cable AC power Analog to LC signal to ChemStation recorder AC power Figure 2 Recommended Stack Configuration (Rear View) 1100 Series DAD and MWD User Manual...
Page 24: Installing The Detector
3 Ensure the line power switch at the front of the detector is OFF. 4 Connect the power cable to the power connector at the rear of the detector. 5 Connect the CAN cable to other Agilent 1100 Series modules. 6 Connect the LAN cable (e.g. from a Agilent ChemStation as controller) to the G1315C/G1365C LAN connector.
Page 25 Installing the Detector 7 Connect the analog cable(s) (optional). 8 Connect the APG remote cable (optional) for non-Agilent 1100 Series instruments. 9 Turn on power by pushing the button at the lower left hand side of the detector. The status LED should be green.
Page 26 The detector was shipped with default configuration settings. To change these settings see N O T E “Configuration Switches” on page 34. 1100 Series DAD and MWD User Manual...
Page 27: Flow Connections To The Detector
Some types of the Agilent deuterium lamps show a light ring during operation. This is not harmful, refer to “UV-Radiation” on page 204. 1100 Series DAD and MWD User Manual...
Page 28 2 Press the release button and open the flow cell door. gain access to the flow cell area. 3 Insert the flow cell. 4 Connect the flow cell capillaries to the capillary holder (top is inlet, bottom is outlet). 1100 Series DAD and MWD User Manual...
Page 29 Installing the Detector 5 If another Agilent 1100 Series module is positioned on top 6 Connect the capillary from the column to the capillary of the detector, route the tubing assembly waste from the holder (top). Connect the teflon waste tubing to the flow...
Page 30: Setting Up The Lan Access
Installing the Detector Setting up the LAN access Please follow the instructions in “LAN Configuration” on page 31 1100 Series DAD and MWD User Manual...
Page 31: Lan Configuration
Agilent 1100 Series Diode Array and Multiple Wavelength Detector SL User Manual LAN Configuration What you have to do first TCP/IP parameter configuration Configuration Switches Initialization mode selection Link configuration selection Automatic configuration with Bootp Configuring the Agilent Bootp service program...
Page 32: What You Have To Do First
6) to a the PC network card using a crossover network cable (point-to-point) or b a hub or switch using a standard LAN cable. Figure 6 Location of LAN interface and MAC label 1100 Series DAD and MWD User Manual...
Page 33: Tcp/Ip Parameter Configuration
TCP/IP parameters after power-on. The parameters may be derived from a Bootp cycle, non-volatile memory or initialized with known default values. The initialization mode is selected by the configuration switch, see Figure 7 on page 34. 1100 Series DAD and MWD User Manual...
Page 34: Configuration Switches
Factory Default Settings Initialization (‘Init’) Mode Bootp, all switches down. For details see “Bootp” on page 35 Link Configuration speed and duplex mode determined by auto-negotiation, for details see “Link configuration selection” on page 39 1100 Series DAD and MWD User Manual...
Page 35: Initialization Mode Selection
Server become the active parameters immediately. In addition, they are stored to the non-volatile memory of the detector. Thus, after a power cycle they are still available. This enables a kind of “bootp once” configuration of the detector. 1100 Series DAD and MWD User Manual...
Page 36 When initialization mode “Using Stored” is selected, the parameters are taken from the non-volatile memory of the detector. The TCP/IP connection will be established using these parameters. The parameters were configured previously by one of the described methods. 1100 Series DAD and MWD User Manual...
Page 37 Since the default IP address is a so-called local address, it will not be routed by any network device. Thus, the PC and the detector must reside in the same subnet. 1100 Series DAD and MWD User Manual...
Page 38 In the “Using Default” mode, the parameters stored in the memory of the detector are not N O T E cleared automatically. If not changed by the user, they are still available, when switching back to the mode “Using Stored”. 1100 Series DAD and MWD User Manual...
Page 39: Link Configuration Selection
Link Configuration speed and duplex mode determined by auto-negotiation manually set to 10 Mbps, half-duplex manually set to 10 Mbps, full-duplex manually set to 100 Mbps, half-duplex manually set to 100 Mbps, full-duplex 1100 Series DAD and MWD User Manual...
Page 40: Automatic Configuration With Bootp
If the Agilent Bootp Service program is not already installed on your PC, then install it from N O T E your Agilent ChemStation CD-ROM, located in folder \Bootp. The screens refer to version B.01.0x 1100 Series DAD and MWD User Manual...
Page 41 Figure 12 Bootp Service Settings 3 Launch the Manager. It will open the Bootp Manager screen, see Figure This shows all network hardware that has been added (initially empty). Figure 13 Bootp Manager 1100 Series DAD and MWD User Manual...
Page 42 MAC address (from label on the instrument) • host name • IP address • comment (instrument name / location) • subnet mask (if different) • gateway (if required) Figure 14 Bootp Manager - Enter your parameter 1100 Series DAD and MWD User Manual...
Page 43 Status: Host IP Address is: 134.40.29.56 Status: Reply to BOOTP Request has been sent Status: BOOTP Request finished processing at outer most layer Figure 16 LogFile - the detector has received the parameter 1100 Series DAD and MWD User Manual...
Page 44 41 (Bootp mode). If you want to store your parameters permanently on the detector (for use without the Agilent Bootp service), refer to “Storing the settings permanently with Bootp” on page 45. 1100 Series DAD and MWD User Manual...
Page 45: Storing The Settings Permanently With Bootp
Table 6 on page 35. 9 Power cycle the detector. The detector can be accessed now via LAN without the Agilent Bootp Service, refer to “PC and Agilent ChemStation Setup” on page 54. 1100 Series DAD and MWD User Manual...
Page 46: Manual Configuration
TELNET Session Non-Volatile Stored Parameter Control Module Figure 17 Manual Configuration (Principle) 1100 Series DAD and MWD User Manual...
Page 47: With Telnet
Handheld Controller (G1323B), or the default IP address (see “Configuration Switches” on page 34). When the connection was established successfully, the detector responds with the following: Figure 19 A connection to the module is made 1100 Series DAD and MWD User Manual...
Page 48 [Enter], where parameter refers to the configuration parameter you are defining, and value refers to the definitions you are assigning to that parameter. Each parameter entry is followed by a carriage return. 1100 Series DAD and MWD User Manual...
Page 49 Initialization mode is Using Stored active TCP/IP settings stored TCP/IP settings in non-volatile memory connected to PC with controller software (e.g. Agilent ChemStation), here not connected Figure 22 Telnet - Change IP settings 1100 Series DAD and MWD User Manual...
Page 50 If the Initialization Mode Switch is changed now to “Using Stored” mode, the instrument N O T E will take the stored settings when the module is re-booted. In the example above it would be 134.40.27.99. 1100 Series DAD and MWD User Manual...
Page 51: With Handheld Controller (G1323B)
2 Press F2 “Configure”, select the module where the LAN cable is installed and press the “Enter” key (Figure 24). Figure 24 Select module 3 Press F1 “LAN” (Figure 25). Figure 25 Select LAN 1100 Series DAD and MWD User Manual...
Page 52 : 0.0.0.0 - stored - IP Address : 134.40.25.220 Subnet Mask : 255.255.248.0 Def. Gateway : 134.40.24.1 -------------------------------- TCP/IP Status : Ready -------------------------------- Controllers : no connections Figure 27 LAN interface Status Page (complete) 1100 Series DAD and MWD User Manual...
Page 53 6 Move to the parameter you want to change, enter the new value and press “Enter”. 7 If you completed your changes, press “Done” to leave the Service section. 8 Press F6 “Done” and restart the module by pressing “OK”. Figure 28 Re-boot screen 1100 Series DAD and MWD User Manual...
Page 54: Pc And Agilent Chemstation Setup
(see also “Using Default” on page 37). FIXED IP address automatic IP address via DHCP (requires special IT setup of the network) Figure 29 Changing the TCP/IP settings of the PC 1100 Series DAD and MWD User Manual...
Page 55: Agilent Chemstation Setup
If using a corporate LAN, IP addresses need to be supplied by the responsible IT N O T E department. Also the LAN needs to be able to handle additional traffic. Save the configuration, close the Configuration Editor and start the Agilent ChemStation. 1100 Series DAD and MWD User Manual...
Page 56 LAN Configuration 1100 Series DAD and MWD User Manual...
Page 57 Agilent 1100 Series Diode Array and Multiple Wavelength Detector SL User Manual Using the Detector Setting up an Analysis Before Using the System Requirements and Conditions Optimization of the System Preparing the HPLC System Running the Sample and Verifying the Results...
Page 58: Using The Detector
(when available in the system) and the pump. Solvents containing volatile ingredients will slightly lose these. Therefore priming of the pumping system is required before starting an application. 1100 Series DAD and MWD User Manual...
Page 59 2 Flush all tubes with at least 30 ml of solvent. 3 Set flow to required value of your application and close the purge valve. Pump for approximately 10 minutes before starting your application. 1100 Series DAD and MWD User Manual...
Page 60: Requirements And Conditions
Zorbax Eclipse XDB-C18, 4.6 x 150 mm, 5 um Part No. 993967-902 or Part No. 5063-6600 Standard: Part No. 01080-68704 0.15 wt.% dimethylphthalate, 0.15 wt.% diethylphthalate, 0.01 wt.% biphenyl, 0.03 wt.% o-terphenyl in methanol 1100 Series DAD and MWD User Manual...
Page 61 Table 12 Conditions Flow: 1.5 ml/minute Stoptime: 8 minutes Solvent: 100% (30% water/70% Acetonitrile) Temperature Ambient Wavelength: sample 254 nm (4 nm bandwidth) reference 360 nm (100 nm bandwidth) Injection Volume 1 µl 1100 Series DAD and MWD User Manual...
Page 62: Optimization Of The System
The settings used for this analysis are specific for this purpose. For other applications the system can be optimized in various ways. Please refer to the section “Optimizing the Detector” on page 84 or “Optimizing” in the your module’s Reference Manual. 1100 Series DAD and MWD User Manual...
Page 63: Preparing The Hplc System
Preparing the HPLC System 1 Turn on the Agilent ChemStation PC and the monitor. 2 Turn on the 1100 series HPLC modules. 3 Start the Agilent ChemStation software. If the pump, autosampler and detector are found, the ChemStation screen should look like shown in...
Page 64 The detector status green (lamp(s) ignited). The System status is green (Ready). Figure 33 Turning on the HPLC Module 5 Purge the pump. For more information see “Priming and Purging the System” on page 58. 1100 Series DAD and MWD User Manual...
Page 65 Stabilization of Baseline (both lamps turned on at the same time) Table 13 Baseline drift after lamp turn on (example from Figure Time [minutes] Drift [mAU/hr] 17 - 20 27 - 30 37 - 40 47 - 50 57 - 60 < 0.2 1100 Series DAD and MWD User Manual...
Page 66 OK. The default LC method parameters are transferred into the 1100 modules. After turn on: The detector status green (lamp(s) ignited). The System status is green (Ready). Figure 35 Loading Default LC Method 1100 Series DAD and MWD User Manual...
Page 67 68 shows the detector settings (do not change the detector parameters at this time). Figure 36 Open the module menu 10 Enter the pump parameters mentioned under “Conditions” on page 61. 1100 Series DAD and MWD User Manual...
Page 68 Signals“ in the Service Manual. • time table for programmable actions during the run. NOTE: The Agilent G1315C DAD and G1365C MWD time table can contain a maximum of 60 rows. Figure 37 Detector parameter (default) 1100 Series DAD and MWD User Manual...
Page 69 Change the Y-range for the DAD to 1 mAU and the offset to 20% and the pressure offset to 50%. The X-axis range should be 15 minutes. Press OK to exit this screen. Figure 38 Edit Signal Plot Window 1100 Series DAD and MWD User Manual...
Page 70 13 If both baselines are stable, set the Y-range for the detector signal to 100 mAU. If you start with a new UV-lamp for the first time, the lamp may show initial drift for some N O T E time (burn-in effect). 1100 Series DAD and MWD User Manual...
Page 71 Figure 40 Sample Information 15 Fill the content of an isocratic standard sample ampoule into a vial and seal the vial with a cap and place the vial into autosampler tray (position #1). 1100 Series DAD and MWD User Manual...
Page 72: Running The Sample And Verifying The Results
ChemStation will show the resulting chromatogram. Figure 41 Chromatogram with Isocratic Test Sample Information about using the Data Analysis functions can be obtained from the Using your N O T E ChemStation manual supplied with your system. 1100 Series DAD and MWD User Manual...
Page 73: Special Settings Of The Detector
100 mV or 1 V full scale, see also “Analog Output Settings” on page 79. • Automatic Turn On: lamps can be programmed (detector must be on for this). • Help: online help. Figure 42 Detector control settings 1100 Series DAD and MWD User Manual...
Page 74: Configuration Settings
N O T E Temperature Control to off. This will lower the optical unit and flow cell temperature by some degree C. For more details see “Principle of Temperature Control” in the Service Manual. 1100 Series DAD and MWD User Manual...
Page 75: Online Spectra
Using the Detector Online Spectra 1 To view the online spectra during the run select Online Spectra. Figure 44 Online Spectra Window 2 Change the absorbance and wavelength range according your needs. 1100 Series DAD and MWD User Manual...
Page 76: Run Recovery Settings
When during recovery an error “Method/Sequence stopped” appears, the instrument N O T E logbook shows an entry “No Run data available in device“. In this case refer to “No Run Data Available In Device” in the Service Manual. 1100 Series DAD and MWD User Manual...
Page 77 If the detector status window is not opened, you will realize only the Power Fail error and N O T E the long Run In Progress information until the data is recovered from disk. 1100 Series DAD and MWD User Manual...
Page 78 When during recovery an error “Method/Sequence stopped” appears, the instrument N O T E logbook shows an entry “No Run data available in device“. In this case refer to “No Run Data Available In Device” in the Service Manual. 1100 Series DAD and MWD User Manual...
Page 79: Analog Output Settings
Zero Offset Limits: 1 to 99% in steps of 1% Attenuation Limits: 0.98 to 2000 mAU at discrete values for either 100 mV or 1 V full scale. Figure 46 Online Spectra Window 3 Change the values if required. 1100 Series DAD and MWD User Manual...
Page 80: Spectrum Settings
2 In the section Spectrum click on the drop-down list. 3 Change the Range, Step width and Threshold according to your needs. Table 15 on page 81 shows the possible parameters. Figure 47 Spectra Window 1100 Series DAD and MWD User Manual...
Page 81 Limits: 0.001 to 1000.00 mAU in steps of 0.001 mAU. Usable for modes Apex + Baselines, Apex + Slopes + Baselines and All in Peak 1100 Series DAD and MWD User Manual...
Page 82: Peakwidth Settings
Limits: When you set the peak width (in minutes), the corresponding response time is set automatically and the appropriate data rate for signal and spectra acquisition is selected as shown in Table 16 on page 83. Figure 48 Peakwidth Setting 1100 Series DAD and MWD User Manual...
Page 83: Slit Settings
The smaller the optical bandwidth the higher the spectral resolution. To set the slit width, display the drop-down list and select an appropriate slit width from the list. Figure 49 Slit Settings 1100 Series DAD and MWD User Manual...
Page 84: Margin For Negative Absorbance Settings
The higher the value the greater the baseline noise. Set this value only if you expect N O T E negative absorbance greater than -100 mAU. Optimizing the Detector Additional theoretical information can be found in chapter “How to optimize the Detector” on page 87: 1100 Series DAD and MWD User Manual...
Page 85: Special Setups With Multiple Dad/Mwds
If you have similar detectors in the same system, the G1315C will be automatically always detector 2 while the G1315A/B is detector 1 independent from its location in the stack. This cannot be changed. 1100 Series DAD and MWD User Manual...
Page 86 Using the Detector 1100 Series DAD and MWD User Manual...
Page 87 Agilent 1100 Series Diode Array and Multiple Wavelength Detector SL User Manual How to optimize the Detector Optimizing the Detector Performance Optimization Overview Optimizing for Sensitivity, Selectivity, Linearity and Dispersion Optimizing Selectivity This chapter provides information on how to optimize the detector.
Page 88: How To Optimize The Detector
How to Get the Best Detector Performance The information below will guide you on how to get the best detector performance. Follow these rules as a start for new applications. It gives rules-of-thumb for optimizing detector parameters. 1100 Series DAD and MWD User Manual...
Page 89: Optimization Overview
Select the reference wavelength with broad bandwidth (30...100 nm) • baseline drift due to RI effects. wavelength range where your analytes have little or no absorbance (e.g. sample at 254 nm, reference at 320 nm). 1100 Series DAD and MWD User Manual...
Page 90 0.2 ... 0.4 0.4 ... 0.4 Typical flow 0.01 ... 0.2 ml/min ml/min ml/min rate ml/min Internal column diameter 0.5 ... 1 mm 2.1 mm 3.0 mm 4.6 mm Figure 51 Choosing a Flow Cell 1100 Series DAD and MWD User Manual...
Page 91: Optimizing For Sensitivity, Selectivity, Linearity And Dispersion
As a rule-of-thumb the flow cell volume should be about 1/3 of the peak volume at half height. To determine the volume of your peaks, take the peak width as reported in the integration results multiply it by the flow rate and divide it by 3). 1100 Series DAD and MWD User Manual...
Page 92 10.00 ± 0.02 mm 10/10 80 Nanoliter flow cell kit 0.08 µl G1315-68716 6 mm 6.00 ± 0.02 mm High Pressure flow cell 1.7 µl G1315-60015 6 mm 5.75 ± 0.07 mm 6/5.75 1100 Series DAD and MWD User Manual...
Page 93: Peak Width (Response Time)
(response time) by factor of two from the recommended setting (over-filtering) will reduce peak height by about 20 % and reduce baseline noise by a factor of 1.4. This gives you the best possible signal-to-noise ratio, but may affect peak resolution. 1100 Series DAD and MWD User Manual...
Page 94: Sample And Reference Wavelength And Bandwidth
• sample wavelength, the center of a wavelength band with the width of sample bandwidth (BW), and optionally • reference wavelength, the center of a wavelength band with the width of reference bandwidth. 1100 Series DAD and MWD User Manual...
Page 95 1.5 AU by setting the sample wavelength to a valley in the spectrum, like 225 nm for anisic acid. Anisic acid sample wavelength 252 nm Reference bandwidth 100 nm 30 nm bandwidth Reference wavelength 360 Wavelength (nm) Figure 54 Optimization of Wavelength Setting 1100 Series DAD and MWD User Manual...
Page 96 This is almost completely eliminated by using a reference wavelength. With this technique, PTH-amino acids can be quantified in the low picomole range even in a gradient analysis. 1100 Series DAD and MWD User Manual...
Page 97: Slit Width
A narrow slit provides spectral resolution for analytes with very fine structures in the absorbance spectrum. An example of such a spectrum is benzene. The five main absorbance bands (fingers) are only 2.5 nm wide and just 6 nm apart from each other. 1100 Series DAD and MWD User Manual...
Page 98 Any photodiode receives light within a range of wavelength determined by the slit width. This explains why the fine spectral structure of benzene disappears when using a 16-nm wide slit. 1100 Series DAD and MWD User Manual...
Page 99 Use a wide (8 or 16 nm) slit when your sample contains very small concentrations. Always use signals with bandwidth at least as wide as the slit width. 1100 Series DAD and MWD User Manual...
Page 100: Optimizing Spectral Acquisition (Dad Only)
4 nm would be sufficient. However a step of 2 nm gives a more optimal display of the spectrum. Threshold Sets the peak detector. Only spectra from peaks higher than threshold will be stored when a peak-controlled storage mode is selected. 1100 Series DAD and MWD User Manual...
Page 101: Margin For Negative Absorbance
(about -100 mAU). In some special case, for example, when gradient with absorbing solvents are used, the baseline may drift to more negative values. Only for such cases, increase the margin for negative absorbance to avoid overflow of the analog-to-digital converter. 1100 Series DAD and MWD User Manual...
Page 102: Optimizing Selectivity
222 nm, where caffeine also shows significant absorbance. It would therefore be impossible, with a conventional variable wavelength detector, to detect hydrochlorothiazide quantitatively when caffeine is present. 1100 Series DAD and MWD User Manual...
Page 103 The trade-off for this procedure is a loss in sensitivity. The sample signal decreases by the absorbance at the reference wavelength relative to the signal wavelength. Sensitivity may be decreased by as much as 10–30 %. 1100 Series DAD and MWD User Manual...
Page 104: Ratio Qualifiers For Selective Detection Of Compound Classes
When the ratio falls within the range, the signal output corresponds to the normal absorbance, giving single, clear peaks on a flat baseline. An example is shown in Figure 61 Figure 1100 Series DAD and MWD User Manual...
Page 105 The characteristic wavelengths 249 nm (λ ) and 224 nm (λ ) were found from the spectra shown in Figure 1100 Series DAD and MWD User Manual...
Page 106 10%). Only when the ratio between 249 and 224 nm was within this range, is the signal plotted. Of all four peaks, only the third fulfilled the criterion (Figure 62). The others were not plotted. 1100 Series DAD and MWD User Manual...
Page 107: Troubleshooting And Diagnostics
Agilent 1100 Series Diode Array and Multiple Wavelength Detector SL User Manual Troubleshooting and Diagnostics Overview of the Detector’s Indicators and Test Functions Status Indicators This chapter gives an overview about the troubleshooting and diagnostic features. Agilent Technologies...
Page 108: Overview Of The Detector's Indicators And Test Functions
Service Manual). Diagnostic Signals The detector has several signals (internal temperatures, voltages and currents of lamps) that can be used for diagnosing baseline problems (see “Diagnostic Signals” in the Service Manual). 1100 Series DAD and MWD User Manual...
Page 109: Status Indicators
Figure 63 Location of Status Indicators Power Supply Indicator The power supply indicator is integrated into the main power switch. When the indicator is illuminated (green) the power is ON. 1100 Series DAD and MWD User Manual...
Page 110: Detector Status Indicator
Usually, an error condition requires attention (e.g. leak, defective internal components). An error condition always interrupts the analysis. • A red-blinking indicator indicates that the module is in resident mode (e.g. during update of main firmware). 1100 Series DAD and MWD User Manual...
Page 111: Maintenance And Repair
Agilent 1100 Series Diode Array and Multiple Wavelength Detector SL User Manual Maintenance and Repair Introduction to Maintenance and Repair Warnings and Cautions Cleaning the Detector Using the ESD Strap This chapter provides general information on repairing the detector. Agilent Technologies...
Page 112: Introduction To Maintenance And Repair
The security lever at the power input socket prevents that the detector cover is taken off when line power is still connected. These repairs are described in “Repairs” in the Service Manual). 1100 Series DAD and MWD User Manual...
Page 113: Warnings And Cautions
Eye damage may result from directly viewing the light produced by the deuterium WA R N I N G lamp used in this product. Always turn the deuterium lamp off before removing it. 1100 Series DAD and MWD User Manual...
Page 114: Cleaning The Detector
Do not use an excessively damp cloth allowing liquid to drip into the detector. Do not let liquid drip into the detector. It could cause shock hazard and it could WA R N I N G damage the detector. 1100 Series DAD and MWD User Manual...
Page 115: Using The Esd Strap
2 Unroll the rest of the band and peel the liner from the copper foil at the opposite end. 3 Attach the copper foil to a convenient and exposed electrical ground. Figure 64 Using the ESD Strap 1100 Series DAD and MWD User Manual...
Page 116 Maintenance and Repair 1100 Series DAD and MWD User Manual...
Page 117: Maintenance
Agilent 1100 Series Diode Array and Multiple Wavelength Detector SL User Manual Maintenance Overview of Maintenance Exchanging a Lamp Exchanging a Flow Cell Repairing the Standard or Semi-Micro Flow Cell Repairing the High Pressure Flow Cell Replacing Capillaries on a Standard Flow Cell...
Page 118: Overview Of Maintenance
A holmium or wavelength calibration Cleaning or exchange test should be performed after replacement. Leak sensor drying If leak has occurred. Check for leaks. Leak handling System If broken or corroded. Check for leaks. replacement 1100 Series DAD and MWD User Manual...
Page 119: Exchanging A Lamp
Press the release buttons and remove the front cover to Disconnect lamp from the connector, unscrew the have access to the lamp area. Vis-lamp (left) and/or UV-lamp (right) and remove the lamp. Do not touch the glass bulb with your fingers. 1100 Series DAD and MWD User Manual...
Page 120 Turn the lamp on and give the lamp 10 minutes to warm up. Perform a “Wavelength Verification and Recalibration” described in the Service Manual (after UV-lamp replacement). Perform an “Intensity Test” as described in the Service Manual. 1100 Series DAD and MWD User Manual...
Page 121: Exchanging A Flow Cell
139 Preparations Turn the lamp(s) off. Remove the front cover. Press the release buttons and remove the front cover to Open the flow cell cover. have access to the flow cell area. 1100 Series DAD and MWD User Manual...
Page 122 If you want to replace flow cell parts, see “Repairing the Standard or Semi-Micro Flow Cell” on page 124 “Repairing the Standard or Semi-Micro Flow Cell” on page 124. 1100 Series DAD and MWD User Manual...
Page 123 “Wavelength Verification and Recalibration” described in the Service Manual to check the correct flow cell (outside of the cell compartment) and all positioning of the flow cell. capillary connections. Replace the front cover. 1100 Series DAD and MWD User Manual...
Page 124: Repairing The Standard Or Semi-Micro Flow Cell
Turn the flow off. Remove the front cover. Remove the flow cell, see “Exchanging a Flow Cell” on page 121. The gaskets used in the standard and semi-micro flow cell are different. N O T E 1100 Series DAD and MWD User Manual...
Page 125 (push through from the screw side). If the washers fall out of the window assembly, they must be inserted in the correct order with the Teflon® ring to prevent any leaks from the flow cell window. 1100 Series DAD and MWD User Manual...
Page 126 9 - flow cell body 10 - inlet capillary 11 - outlet capillary 12 - holder Assemble the washers and the window assembly in Correct orientation of spring washers [2] is required. correct order. 1100 Series DAD and MWD User Manual...
Page 127 Using a 4-mm hex key, tighten the window screw hand into the cell body. Assure, that the gasket is on the bottom. tight plus a quarter turn. Do not mix the gasket #6 and # 7 (different hole diameter) 1100 Series DAD and MWD User Manual...
Page 128 Insert the flow cell. Replace the front cover. Perform a “Wavelength Verification and Recalibration” or a “Holmium Oxide Test” as described in the Service Manual to check the correct positioning of the flow cell. 1100 Series DAD and MWD User Manual...
Page 129: Repairing The High Pressure Flow Cell
If you want to replace the gasket only, continue with Use a 4 mm hex key to unscrew the window assembly [1] step 7. and remove the gasket [2] from the cell body. 1100 Series DAD and MWD User Manual...
Page 130 5 - spring washers 4 - window holder 6 - window screw 7 - inlet capillary 8 - outlet capillary Follow the procedure “Repairing the Standard or Semi-Micro Flow Cell” on page 124 for reassembling. 1100 Series DAD and MWD User Manual...
Page 131: Replacing Capillaries On A Standard Flow Cell
When retightening the fittings, make sure that they are carefully tightened (handtight plus 1/4 turn with a wrench). Otherwise damage of the flow cell body or blockage may result. Inlet Outlet 1100 Series DAD and MWD User Manual...
Page 132 Unscrew the cell body from the heat Unscrew the fixing screw and unwrap the inlet capillary exchanger and the heat exchanger from the grove in the flow cell body. from the clamp unit. Screw Inlet capillary with heat exchanger 1100 Series DAD and MWD User Manual...
Page 133 90° 90° Insert the capillary into the hole between fixing screw and The capillary lays in the grove and should be tied around the inlet fitting. the body (in the grove) 5 times. 1100 Series DAD and MWD User Manual...
Page 134 Inlet capillary with heat Fix the inlet capillary to the flow cell body handtight first. Fix the new heat exchanger to the clamp unit. Then do a 1/4 turn with a 4-mm wrench. 1100 Series DAD and MWD User Manual...
Page 135 Perform a leak test. Insert the flow cell. Replace the front cover. Perform a “Wavelength Verification and Recalibration” “Holmium Oxide Test” as described in the Service Manual to check the correct positioning of the flow cell. 1100 Series DAD and MWD User Manual...
Page 136: Replacing Capillaries On A Semi-Micro And High Pressure Flow Cell
When retightening the fittings, make sure that they are carefully tightened (handtight plus 1/4 turn with a wrench). Otherwise damage of the flow cell body or blockage may result. Outlet capillary 1100 Series DAD and MWD User Manual...
Page 137 Unscrew the cell body from the heat exchanger and the Fix the new heat exchanger to the clamp unit and the heat heat exchanger from the clamp unit. exchanger to the cell body. 1100 Series DAD and MWD User Manual...
Page 138 Insert the flow cell. Inlet capillary Replace the front cover. Perform a “Wavelength Verification and Recalibration” “Holmium Oxide Test” as described in the Service Manual to check the correct positioning of the flow cell. 1100 Series DAD and MWD User Manual...
Page 139: Nano Flow Cell - Replacing Or Cleaning
For details refer to the technical note that comes with the nano-flow cell kit. N O T E The quartz block can be cleaned with alcohol. DO NOT touch the inlet and outlet windows N O T E at the quartz block. 1100 Series DAD and MWD User Manual...
Page 140 Unscrew the capillaries from the flow cell. DO NOT use the Using for example a toothpick, press on the plastic part adapter at this time! and slide the quartz body out of the cell housing. 1100 Series DAD and MWD User Manual...
Page 141 Replace the cell seal assembly onto the quartz body. Slide the quartz body completely into the cell body to the Always use a new seal assembly to exclude damage front stop (use for example a toothpick). during disassembling. 1100 Series DAD and MWD User Manual...
Page 142 The cell body can be fitted in two positions to allow the capillaries routed upwards or N O T E downwards (depending on where the column is located). Route the capillaries directly column (inlet) and waste assembly (outlet). 1100 Series DAD and MWD User Manual...
Page 143 The wrench has to be plugged into the adapter as shown in Figure Adapter Wrench DO NOT press down more than shown here 0.7 Nm Figure 65 Wrench plus Torque Adapter 1100 Series DAD and MWD User Manual...
Page 144: Cleaning Or Exchanging The Holmium Oxide Filter
A slight change in the absorbance might be possible. Other components within the light path (lenses, windows, ...) are also changing their behavior over the time. 1100 Series DAD and MWD User Manual...
Page 145 While releasing the holder with a screw driver (at the top), Note: carefully remove the holmium oxide filter. Do not scratch the holmium oxide filter. The holmium oxide filter can be cleaned with alcohol and a lint-free cloth. 1100 Series DAD and MWD User Manual...
Page 146 Service Manual to check the proper function of the holmium oxide filter. Insert the flow cell, see “Exchanging a Flow Cell” on page 121. Replace the front cover. Turn on the flow. 1100 Series DAD and MWD User Manual...
Page 147: Correcting Leaks
1 Use tissue to dry the leak sensor area and the leak pan. 2 Observe the capillary connections and the flow cell area for leaks and correct, if required. Leak pan Leak sensor assembly Figure 66 Observing for Leaks 3 Replace the front cover. 1100 Series DAD and MWD User Manual...
Page 148: Replacing Leak Handling System Parts
3 Insert the leak funnel with the tubing in its position. 4 Insert the leak funnel into the leak funnel holder. Leak funnel and holder Leak tubing Figure 67 Replacing Leak Handling System Parts 5 Replace the front cover. 1100 Series DAD and MWD User Manual...
Page 149: Replacing The Compactflash Card
1 Remove the CompactFlash card by pulling it out of its slot in the rear of the detector. 2 Install the new CompactFlash card into the slot. 3 Turn the detector ON. Figure 68 Replacing SanDisk CompactFlash card 1100 Series DAD and MWD User Manual...
Page 150: Replacing The Detector's Firmware
Otherwise the communication will not work. 3 If the detector main board was replaced, perform a “Wavelength Verification and Recalibration” as described in the Service Manual to add the recalibration parameters into the board’s memory. 1100 Series DAD and MWD User Manual...
Page 151 4 If detector main board was replaced, re-enter the serial number information of the module through the user interface, see “Using the Control Module G1323B” “Using the Agilent ChemStation” as described in the Service Manual. 1100 Series DAD and MWD User Manual...
Page 152 Maintenance 1100 Series DAD and MWD User Manual...
Page 153: Parts And Materials For Maintenance
Agilent 1100 Series Diode Array and Multiple Wavelength Detector SL User Manual Parts and Materials for Maintenance Overview of Maintenance Parts Standard Flow Cell Semi-Micro Flow Cell High Pressure Flow Cell Nano Flow Cells Accessory Kit This chapter provides information on parts for maintenance.
Page 154: Overview Of Maintenance Parts
Parts and Materials for Maintenance Overview of Maintenance Parts Figure 69 Maintenance Parts 1100 Series DAD and MWD User Manual...
Page 155 Teflon Tubing flexible i.d. 0.8 mm (flow cell to waste), see “Accessory Kit” on page 166 5062-2462 Cable CAN to Agilent 1100 Series modules (0.5 m) 5181-1516 Cable CAN to Agilent 1100 Series modules (1 m) 5181-1519 Cross-over network cable (shielded, 3 m long) for point to point connection...
Page 156: Standard Flow Cell
Seal kit FRONT for STD flow cell, qty=12 of item 7 G1315-68710 Window screw kit, includes 2 quartz windows, 2 compression 79883-68703 washers, 2 window holders, 2 window screws and 10 spring washers 1100 Series DAD and MWD User Manual...
Page 157 N O T E 1 - window screw 2 - spring washers 3 - compression washer 4 - window holder 5 - quartz window 6 - Gasket Figure 71 Orientation of Spring Washers. 1100 Series DAD and MWD User Manual...
Page 158: Semi-Micro Flow Cell
Seal kit FRONT for semi-micro flow cell, qty=12 of item 7 G1315-68710 Window screw kit, includes 2 quartz windows, 2 compression 79883-68703 washers, 2 window holders, 2 window screws and 10 spring washers 1100 Series DAD and MWD User Manual...
Page 159 N O T E 1 - window screw 2 - spring washers 3 - compression washer 4 - window holder 5 - quartz window 6 - Gasket Figure 73 Orientation of Spring Washers. 1100 Series DAD and MWD User Manual...
Page 160: High Pressure Flow Cell
Screw M 2.5, 4 mm lg for cell body/clamp unit 0515-1056 Capillary (0.12 mm, 150 mm lg) G1315-87312 Capillary (0.17 mm, 380 mm lg) G1315-87311 Cell Repair Kit Agilent, includes 1 quartz window, 79883-68700 5 spring washers, 2 seal rings 1100 Series DAD and MWD User Manual...
Page 161 Parts and Materials for Maintenance Figure 74 High Pressure Flow Cell Assembly Parts 1100 Series DAD and MWD User Manual...
Page 162: Nano Flow Cells
(includes items 1, 2, 3, 4, 10, 11, 12, 13, 14, 15, and 16) Figure 75 shows all parts delivered with the nano-flow cell kits. 3, 4 3, 4 Figure 75 Content of kits 1100 Series DAD and MWD User Manual...
Page 163 Handle for clamp unit G1315-84902 Clamp unit G1315-84901 Screw M 2.5, 4 mm long for cell body/clamp 0515-1056 Wrench open end 4 mm (supplied with standard accessory kit 8710-1534 G1315-68705 of your detector) 1100 Series DAD and MWD User Manual...
Page 164 (#3), plus one PEEK Fitting FT (#5) Cell Housing (500 nl) G1315-27703 Cell Seal Assembly (500 nl) G1315-87101 Quartz Body (500 nl) G1315-80001 Sealing Kit, includes items #10, #12 (QTY=2) and #7 (QTY=5) G1315-68715 1100 Series DAD and MWD User Manual...
Page 165 Cell Housing (80 nl) G1315-27704 Cell Seal Assembly (80 nl) G1315-42301 Quartz Body (80 nl) G1315-80002 Sealing Kit 80 nl cell, includes items #10, #12 (QTY=2), #7 (QTY=5)and G1315-68725 Lite Touch Sleeves (QTY=5) 1100 Series DAD and MWD User Manual...
Page 166: Accessory Kit
Wrench open end 1/4 – 5/16 inch 8710-0510 Wrench open end 4 mm 8710-1534 ESD wrist strap 9300-1408 * or kit 5062-2418 1/16" Fittings and Ferrules (front/back) 10/PK For item number refer to Figure 76 Figure 1100 Series DAD and MWD User Manual...
Page 167 Parts and Materials for Maintenance Figure 76 Waste Tubing Parts This end is pre-installed Figure 77 Inlet Capillary (Column-Detector) Parts 1100 Series DAD and MWD User Manual...
Page 168 Parts and Materials for Maintenance 1100 Series DAD and MWD User Manual...
Page 169: Control Module Screens
Agilent 1100 Series Diode Array and Multiple Wavelength Detector SL User Manual Control Module Screens Major keys on the Agilent 1100 Control Module Control Module - Unsupported Module Screens available from the Analysis screen Screens available from the System screen...
Page 170: Major Keys On The Agilent 1100 Control Module
In case the control module’s display seems to be frozen (hang-up due to a communication N O T E problem on the CAN bus, unplug the control module from the LC module and reconnect. 1100 Series DAD and MWD User Manual...
Page 171: Control Module - Unsupported Module
In case the control module shows the following screen and the yellow LED on the detector is not flashing, the used firmware in the Control Module is not able to control the G1315C DAD or G1365C MWD. These detectors require firmware revision B.04.01 or above (G1323B). 1100 Series DAD and MWD User Manual...
Page 172: Screens Available From The Analysis Screen
Agilent 1100 module. It is used to enter the most common detector method parameters. The m-key allows access to the context sensitive menu. Setup view leads you to add sections for additional Agilent 1100 modules. Restart re-boots the control module. 1100 Series DAD and MWD User Manual...
Page 173 Use the F8 key (On/Off) to turn on the lamp(s). If more than one module is available, select the DAD or MWD from the pop-up menu. The lamp(s) can be selected individually. Setup View In the Setup view, e.g. another module can be added to the view. 1100 Series DAD and MWD User Manual...
Page 174 Maximum 4 modules are shown automatically. If more modules are in the system, you have to chose in Setup view. With the Settings key you open a pull-down menu where you can select the DAD modules. 1100 Series DAD and MWD User Manual...
Page 175 Use the m-key for the context sensitive menu. The Status command pulls up a monitor screen displaying signals and spectra as programmed. Reset will load the DAD or MWD default parameters. Balance brings the baseline back to set offset. 1100 Series DAD and MWD User Manual...
Page 176 Selecting More and Settings pull down menu you can enter special DAD or MWD setpoints. Selecting More and Signals C-E or Signals F-H on the pull down menu you can enter additional DAD or MWD signal parameters. 1100 Series DAD and MWD User Manual...
Page 177 Press the F7 key (Insert) to add a timetable events. Use the F6 key (Done) to view the entered lines of the timetable. Use the m-key for the context sensitive menu. It gives you additional tools for the timetable. 1100 Series DAD and MWD User Manual...
Page 178 FOR G1315A/B DAD: With the F4 key (Spectrum) you can change the settings for the spectrum acquisition. FOR G1365A/B MWD: You can change the settings for the spectrum acquisition (STOP FLOW). The spectrum is started via “Sample, Holmium and Dark Current Spectrum” on page 193. 1100 Series DAD and MWD User Manual...
Page 179 With the F5 key (Runtimes) you can change the stop time and the post-run time. Press F5 key (Views) and select Status. Status This is an example if an Agilent 1100 DAD or MWD is configured standalone. 1100 Series DAD and MWD User Manual...
Page 180 Use the Right/Left arrows to switch between Available and Selected Signals. Use the F8 key (Move) to enter available signals into the box for selected signals or vice versa. Press F6 key (Setup) to change the signal setting. 1100 Series DAD and MWD User Manual...
Page 181 PC-Card and Instrument window. Use the UP/Down arrows to select the method. Use the F7/F8 keys (Copy) to enter available signals into the box for selected signals or vice versa. 1100 Series DAD and MWD User Manual...
Page 182: Screens Available From The System Screen
System - Control Use the F1 key (Control) to select the DAD or MWD. Here you receive information about the not-ready conditions if needed. F2 key (Reset) does a re-initialization of the DAD or MWD. 1100 Series DAD and MWD User Manual...
Page 183 Use the F1 key (LAN) to access the LAN interface settings. The TCP Status Page displays the current settings (MAC address of the LAN interface, Init Mode and the TCP/IP information). Use the F1 key (Service) to change the TCP/IP settings. 1100 Series DAD and MWD User Manual...
Page 184 “use all UV-lamps“ if UV-lamp has no I.D. tag. Use the F4 key (Cell Tag) to configure the flow cell tag reader. Use option “use Cell anyway“ if flow cell has no I.D. tag. 1100 Series DAD and MWD User Manual...
Page 185: Screens Available From The Records Screen
Use the Esc key to receive Views on the F5 key. Choose System from the pull-down menu. Use the F4 key (Records) to select the DAD or MWD. System / Error Log Errors are reported either into the System Log (F2) or Error Log (F3). 1100 Series DAD and MWD User Manual...
Page 186 Use the m-key to receive a pop-up menu, Select Info Log. A list of the last events are listed. For troubleshooting reasons they can be printed or saved to a file on the PCMCIA card. 1100 Series DAD and MWD User Manual...
Page 187 If a set limit has been exceeded, a message box will pop up. This will not stop a sequence or run (information only to plan maintenance activities). If you press Reset, the limits will be removed. Ignore will continue to keep the EMF flag set. 1100 Series DAD and MWD User Manual...
Page 188 PCMCIA card (res_B101_bb.dlb) and press execute. If you want to update the main firmware, press F7 key (Transfer) to turn the module into the resident mode (LED on module should blink). 1100 Series DAD and MWD User Manual...
Page 189 (Transfer) to return the module into the normal mode (LED on module should stay yellow). If you have not saved your methods, please do it before continuing. Otherwise they will be overwritten during the update process. 1100 Series DAD and MWD User Manual...
Page 190 In case the serial number of the module has to be added, use the m-key to open the menu Enter Serial#. Press Done and reboot the detector to make the new serial number active. 1100 Series DAD and MWD User Manual...
Page 191 Use the F4 key (Maint log) to view and edit the maintenance logbook. Use the F7 key (Add) to add new maintenance activities. If an activity is not listed, you can type the activity into the line “Add” using the control modules key pad. 1100 Series DAD and MWD User Manual...
Page 192: Diagnostics And Tests
Service Manual for more information on the tests. Intensity Test Use the F5 key (Intensity) to get the instrument profile (example with UV and Vis lamp on). To redo the test press key F8 (Take). 1100 Series DAD and MWD User Manual...
Page 193 Sample, Holmium and Dark Current Spectrum Use the F2 key (Spectrum) to take a spectrum (sample, dark current or holmium). Use the Left/Right arrow to move the curser within the spectrum to find the wavelength of interest. 1100 Series DAD and MWD User Manual...
Page 194 Use the F6 key (Plot) to view the signal. A simulated chromatogram (4 peaks) will repeat until the run is stopped. The signal is also available on the analog output. A change of the peak width will change the retention times. 1100 Series DAD and MWD User Manual...
Page 195 Use the F3 key (Slit Test) to test the movement of the electromechanical slit assembly. Part Info Use the F4 key (Part Info) to display the ID tag information of the UV-lamp assembly or the flow cell assembly (for lamps/cells with ID tag only). 1100 Series DAD and MWD User Manual...
Page 196 Control Module Screens 1100 Series DAD and MWD User Manual...
Page 197 Agilent 1100 Series Diode Array and Multiple Wavelength Detector SL User Manual Appendix General Safety Information The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC) Radio Interference Sound Emission UV-Radiation Solvent Information Declaration of Conformity for HOX2 Filter Agilent Technologies on Internet This chapter provides addition information on safety, legal and web.
Page 198: Appendix
Make sure that only fuses with the required rated current and of the specified type (normal blow, time delay, and so on) are used for replacement. The use of repaired fuses and the short-circuiting of fuseholders must be avoided. 1100 Series DAD and MWD User Manual...
Page 199 When working with solvents please observe appropriate safety procedures (e.g. goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet by the solvent vendor, especially when toxic or hazardous solvents are used. 1100 Series DAD and MWD User Manual...
Page 200 A caution alerts you to situations that could cause a possible loss of data. Do not C A U T I O N proceed beyond a caution until you have fully understood and met the indicated conditions. 1100 Series DAD and MWD User Manual...
Page 201: The Waste Electrical And Electronic Equipment (Weee) Directive (2002/96/Ec)
I, this product is classed as a “Monitoring and Control instrumentation” product. Do not dispose off in domestic household waste To return unwanted products, contact your local Agilent office, or see www.agilent.com for more information. 1100 Series DAD and MWD User Manual...
Page 202: Radio Interference
If test and measurement equipment is operated with equipment unscreened cables and/or used for measurements on open set-ups, the user has to assure that under operating conditions the radio interference limits are still met within the premises. 1100 Series DAD and MWD User Manual...
Page 203: Sound Emission
This product has a sound pressure emission (at the operator position) < 70 dB. • Sound Pressure Lp < 70 dB (A) • At Operator Position • Normal Operation • According to ISO 7779:1988/EN 27779/1991 (Type Test) 1100 Series DAD and MWD User Manual...
Page 204: Uv-Radiation
Typically the radiation values are much smaller than these limits: Table 33 UV-Radiation Typical Values Position Effective Irradiance Lamp installed, 50 cm distance Average 0.016 µW/cm Lamp installed, 50 cm distance Maximum 0.14 µW/cm 1100 Series DAD and MWD User Manual...
Page 205: Solvent Information
• Halogenated solvents or mixtures which form radicals and/or acids, for example: → 2COCl 2CHCl + 2HCl This reaction, in which stainless steel probably acts as a catalyst, occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol. 1100 Series DAD and MWD User Manual...
Page 206 For example, a 1-% solution of acetic acid in methanol will attack steel. • Solutions containing strong complexing agents (for example, EDTA, ethylene diamine tetra-acetic acid). • Mixtures of carbon tetrachloride with 2-propanol or THF. 1100 Series DAD and MWD User Manual...
Page 207: Declaration Of Conformity For Hox2 Filter
Appendix Declaration of Conformity for HOX2 Filter 1100 Series DAD and MWD User Manual...
Page 208: Agilent Technologies On Internet
For the latest information on products and services visit our worldwide web site on the Internet at: http://www.agilent.com Select “Life Sciences & Chemical Analysis Solutions” under “Support” It will provide also the latest firmware of the Agilent 1100 series modules for download. 1100 Series DAD and MWD User Manual...
Page 209 TCP/IP parameter configuration, test chromatogram, using default, tests, using default parameters, correction factors for flow cells, high pressure flow cell using stored, dimensions, what you have to do first, parts, leak holmium oxide correcting, 1100 Series DAD and MWD User Manual...
Page 210 CompactFlash card, spectra replacing firmware, acquisition, parts identification replacing leak handling system, settings, accessory kit, stack configuration, 22, 1100 Series DAD and MWD User Manual...
Page 211 Control Module, test functions, tests on control module, time table, troubleshooting error messages, status indicators, 108, unpacking, unsupported Module, uv-radiation, wavelength accuracy, bunching, range, recalibration, 108, weight and dimensions, 1100 Series DAD and MWD User Manual...
Page 212 Index 1100 Series DAD and MWD User Manual...
Page 214 In This Book This manual contains the user information about the Agilent 1100 Series Diode Array and Multiple Wavelength Detector The manual describes the following: • installing the detector, • introduction to the diode array detector and its optimization, •...

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