Getting Started And Checkout - Agilent Technologies 1100 Series Reference Manual

2 First Steps with the Fluorescence Detector
For highest reproducibility and linearity change the lamp setting to always ON (default is on only during
run).
NOTE
One hour of initial warm-up of the instrument is recommended.
6 Do not overpressurize the detector quartz flow cell
When comparing fluorescence excitation spectra directly with DAD spectra or literature
NOTE
based absorbance spectra, you should consider large differences in the used optical
bandwidth (FLD=20 nm) which cause a systematic wavelength maximum shift depending on the
absorbance spectrum of the compound under evaluation.

Getting Started and Checkout

This chapter describes the check out of the Agilent 1100 series fluorescence detector
using the Agilent isocratic checkout sample.
When required
Hardware required LC system with G1321A FLD
Parts required
34 1100 Series FD Reference Manual
Unless maximum sensitivity is needed, the lamp lifetime can significantly be
increased by switching it on just for analysis. In contrast to other LC detectors the
G1321A fluorescence detector equilibrates within seconds after the lamp is
switched on.
Be aware to not exceed a 20 bar pressure drop after the flow cell when hooking up
additional devices like other detectors or a fraction collector. It's better to place a
UV detector before the G1321A fluorescence detector.
If you want to checkout the
detector
Start-up Kit 5063-6528, includes
LC cartridge Hypersil ODS, 5um, 125x4mm with CIS cartridge holder
Agilent isocratic checkout sample (Part number 01080-68704)
Fittings, Qty=2 (Part number 0100-1516)
Capillary, 150 mm long, 0.17 mm i.d. (Part number 5021-1817)