Page 1 Minifors 2 Operating manual We bring life to your laboratory.
Page 2 Minifors 2 – Rel. 2.1 Bench-Top Bioreactor SW: 3.5 Doc-ID: D012, 1, en_GB – Original Art. 78972 More information about the product is INFORS HT available online at: Headoffice, Switzerland www.infors-ht.com/de/minifors2 Rittergasse 27 CH-4103 Bottmingen T +41 (0)61 425 77 00 info@infors-ht.com...
Page 3: Supplemental Directives
Minifors 2 - Operating manual Supplemental directives Supplemental directives About this Manual This manual enables the safe and efficient handling of the device. All the information and instructions in this operating manual comply with the current standards, legal regulations and the latest technological developments.
Page 4: Table Of Contents
Intended Use, Incorrect Use and Misuse............. Qualified Personnel......................... 1.3.1 Operator..........................1.3.2 Technician........................1.3.3 INFORS HT Service Technician or Licensed Dealer..Unauthorised Persons......................Responsibility of the Provider..................Residual Risks..........................Warning Symbols on the Device................Declaration of Decontamination................2 Setup and Function..........................20 Basic Unit.............................
Page 5 Minifors 2 - Operating manual Table of contents 2.2 Culture Vessel..........................29 2.2.1 Overview.......................... 29 2.2.2 Top Plate........................... 30 2.2.3 Ports in the Vessel Top Plate..............2.3 Temperature Control System..................34 2.4 Stirrer................................ 2.4.1 Overview.......................... 35 2.4.2 Motor............................ 35 2.4.3 Impeller..........................
Page 6 Minifors 2 - Operating manual Table of contents 4.10 Dip Tubes............................. 4.11 Immersion Pocket for Temperature Sensor (Pt100)....... 58 4.12 Exit Gas Cooler..........................59 4.13 Cold Finger............................4.14 Reagent Bottles........................... 62 4.15 Sampling System Super Safe Sampler............63 4.16 Pump Heads............................. 65 4.17 Vessel Holder..........................
Page 7 Minifors 2 - Operating manual Table of contents 7.1.3 Mounting Dip Tubes and Spargers..........84 7.1.4 Inserting the Vessel into the Vessel Holder......85 7.1.5 Inserting the Baffles.................... 86 7.1.6 Moistening/Filling the Culture Vessel.......... 86 7.1.7 Mounting the Top Plate................
Page 8 Minifors 2 - Operating manual Table of contents Connecting the Culture Vessel and Preparing the Cultiva- tion................................110 7.3.1 Hanging the Culture Vessel in Place and Fitting the Pump Heads......................... 110 7.3.2 Filling the Reagent Hoses................110 7.3.3 Connecting the Gassing................
Page 9 Minifors 2 - Operating manual Table of contents 9.1.4 START BATCH / INOCULATE / STOP BATCH......131 9.1.5 SAMPLE NOW......................132 9.2 Menus for System Settings................... 134 9.2.1 Overview.......................... 134 9.2.2 VESSEL TYPE – Selecting a Culture Vessel......135 9.2.3 APPEARANCE –...
Page 10 Minifors 2 - Operating manual Table of contents 9.6 EXIT GAS Parameter Group................... 163 9.6.1 Exit Gas O ........................163 9.6.2 Exit Gas CO ........................163 9.7 PUMPS Parameter Group....................164 9.7.1 Overview.......................... 164 9.7.2 Configuring the Pumps................... 165 9.7.3 Pump1...........................
Page 11 Minifors 2 - Operating manual Table of contents 10.3.6 Removing Addition Port Adapters, Feed Needle and Temperature Sensor Immersion Pocket....... 192 10.3.7 Removing the Vessel Top Plate............192 10.3.8 Removing the Sparger and the Dip Tube(s)......193 10.3.9 Removing the Impellers................. 193 10.4 Cleaning and Storing Individual Parts..............
Page 12 Minifors 2 - Operating manual Table of contents 13.3 Connections and Connection Values............... 217 13.3.1 Electrical Connection and Power Values......... 217 13.3.2 Water............................ 218 13.3.3 Process Gas........................219 13.4 Specifications of the Basic Unit................219 13.4.1 Operating Panel....................... 219 13.4.2 Culture Vessel......................219 13.4.3 Stirrer............................
Page 13: Safety And Responsibility
Minifors 2 - Operating manual Safety and Responsibility Safety and Responsibility This chapter contains general information on safety when using the device. In the remaining chapters, warning messages are used only to highlight particular hazards directly arising from the actions being described.
Page 14: Intended Use, Incorrect Use And Misuse
Safety and Responsibility Intended Use, Incorrect Use and Misuse Intended Use The bench-top bioreactor Minifors 2 from INFORS HT is designed especially for running bio processes with microorganisms or animal cells for research and development in a biotechnology laboratory. WARNING The device is designed and built exclusively for the intended use described above.
Page 15: Unauthorised Persons
Disassembly, disposal and recycling specialists ■ 1.3.3 INFORS HT Service Technician or Licensed Dealer Certain work may only be performed by the manufacturer's skilled per- sonnel or by skilled personnel authorised by a licensed dealer. Other persons are not authorised to perform this work.
Page 16: Responsibility Of The Provider
Minifors 2 - Operating manual Safety and Responsibility Unauthorised persons are not permitted to operate the device or use it in any other way. Responsibility of the Provider Provider The term “provider” applies to all persons who are responsible for making the device and the necessary infrastructure available.
Page 17 Minifors 2 - Operating manual Safety and Responsibility Electric Current The device is operated electronically. There is an immediate risk of fatal injury if contact is made with live parts. The following points must be observed in order to avoid the risk of fatal injury: In case of damage to insulation, disconnect the device from the ■...
Page 18: Warning Symbols On The Device
Minifors 2 - Operating manual Safety and Responsibility Corrosive or Toxic Substances The use or production of corrosive or toxic substances entails a signifi- cant health risk. As such, special measures must be taken to protect personnel. Check liquid-carrying hoses for leaks at regular intervals.
Page 19: Declaration Of Decontamination
Minifors 2 - Operating manual Safety and Responsibility Warning symbol Position Meaning Thermal block Hot surfaces ■ adapter Motor (version for ■ microorganisms) WARNING Illegible or missing warning symbols on the device will lead to the personnel being exposed to risks that the warning symbols in ques- tion were designed to make them aware of.
Page 20: Setup And Function
Minifors 2 - Operating manual Setup and Function Kopiervorlagen diverse Setup and Function Basic Unit Kopiervorlagen diverse 2.1.1 Overview Kopiervorlagen diverse Kopiervorlage Bilderrahmen Kopiervorlagen diverse Kopiervorlage Bilderrahmen Kopiervorlagen diverse Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen Operating panel Hooks for vessel holder...
Page 21: Operating Panel
Minifors 2 - Operating manual Setup and Function 2.1.2 Operating Panel The operating panel on the top right of the basic unit has a 7” TFT touch screen. On the right-hand side of the panel is a USB port. ■...
Page 22: Mains Connection
Minifors 2 - Operating manual Setup and Function 2.1.5 Mains Connection The mains connection is located at the bottom left of the back of the basic unit. The device is protected against excessive current con- sumption by two fuses. The device fuses are located directly above the mains connection.
Page 23: Gas Connections
Minifors 2 - Operating manual Setup and Function 2.1.7 Gas Connections The connections for the gas supply are located on the rear of the basic unit, at the bottom right, above the water connections. They are marked with the following symbols:...
Page 24 Minifors 2 - Operating manual Setup and Function Symbol Designation Function SERVICE 9-pin RS232 for connecting a diagnostic computer for maintenance. BALANCE 9-pin RS232 for connecting a balance. Port for connecting a network cable. Pis Assignment Plug ANALOG I/O +Analogue Out A...
Page 25: Motor Cable Connection
Minifors 2 - Operating manual Setup and Function 2.1.9 Motor Cable Connection The connection for the motor cable is located on the rear of the basic unit at the top left, and marked with a corresponding symbol. 2.1.10 Connections for Sensors (Sensor Cables)
Page 26: Gassing Connection
Minifors 2 - Operating manual Setup and Function 2.1.11 Gassing Connection The connections for gassing are located on the front of the basic unit, at the bottom left. They are marked with a corresponding symbol. Symbol Function Connection for gassing via sparger.
Page 27: Pumps
Minifors 2 - Operating manual Setup and Function Symbol Function Water outlet exit gas cooler Valve for Water Flow The manual valve for setting the water flow is located on the rear of GAS COOLER and marked with a corre- the basic unit, labelled with sponding symbol.
Page 28: Identification Plate
Minifors 2 - Operating manual Setup and Function The autoclavable pump heads are plugged into a mounting plate (depicted separately here in order to show the marking under the pumps). The pump heads and the mounting plate can be simply pushed onto or pulled off the drive shafts.
Page 29: Culture Vessel
Minifors 2 - Operating manual Setup and Function Kopiervorlagen diverse Culture Vessel 2.2.1 Overview Kopiervorlagen diverse Kopiervorlage Bilderrahmen Kopiervorlagen diverse Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen Kopiervorlagen diverse Kopiervorlage Bilderrahmen Motor coupling Pump holder Vessel holder handle Reagent bottle holder Glass vessel...
Page 30: Top Plate
Minifors 2 - Operating manual Setup and Function Kopiervorlagen diverse 2.2.2 Top Plate Kopiervorlagen diverse Kopiervorlagen diverse Kopiervorlage Bilderrahmen Kopiervorlagen diverse Kopiervorlagen diverse Kopiervorlage Bilderrahmen Kopiervorlagen diverse Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen Knurled nut (4 x) Top plate...
Page 31: Ports In The Vessel Top Plate
Minifors 2 - Operating manual Setup and Function 2.2.3 Ports in the Vessel Top Plate The vessel top plate has different ports of different sizes to mount the different components such as sparger, blanking plugs, sensors etc. The number of ports in the top plate and its configuration depends on the Kopiervorlagen diverse nominal diameter (DN = inner diameter) of the culture vessel.
Page 32 Minifors 2 - Operating manual Setup and Function Kopiervorlagen diverse Vessel Top Plate, DN 115 Kopiervorlagen diverse Kopiervorlagen diverse Kopiervorlage Bilderrahmen Kopiervorlagen diverse Kopiervorlagen diverse Kopiervorlage Bilderrahmen Kopiervorlagen diverse Kopiervorlage Bilderrahmen Kopiervorlagen diverse Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen Ø 12 mm Pg13.5: pH sensor Ø...
Page 33 Minifors 2 - Operating manual Kopiervorlagen diverse Setup and Function Kopiervorlagen diverse Vessel Top Plate, DN 145 Kopiervorlagen diverse Kopiervorlagen diverse Kopiervorlage Bilderrahmen Kopiervorlagen diverse Kopiervorlage Bilderrahmen Kopiervorlagen diverse Kopiervorlage Bilderrahmen Kopiervorlagen diverse Kopiervorlage Bilderrahmen Kopiervorlagen diverse Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen Ø...
Page 34: Temperature Control System
Minifors 2 - Operating manual Setup and Function Temperature Control System Thermal block Thermal block adapter Fastening screw, 4 x Hook, 2 x The temperature (heating and cooling) is controlled using a thermal block and its adapter. There is a thermal block adapter for each vessel size. The thermal block adapters are screwed onto the thermal block.
Page 35: Stirrer
Minifors 2 - Operating manual Setup and Function ervorlagen diverse Stirrer 2.4.1 Overview Drive hub Stirrer shaft Mechanical seal The stirrer shaft is driven from above and turns counter-clockwise. ervorlagen diverse The stirrer shaft is sealed using a mechanical seal.
Page 36 Minifors 2 - Operating manual Setup and Function Version for Cell Cultures The same brushless gear motor with mechanical coupling is used for all vessel sizes. Coupling/Uncoupling the Motor The motor is connected by pushing it onto the drive hub on the top plate.
Page 37: Impeller
Minifors 2 - Operating manual Setup and Function 2.4.3 Impeller Version for Microorganisms Two Rushton impellers are attached to the stirrer shaft by means of grub screws. Version for Cell Cultures One pitched blade impeller is attached to the stirrer shaft by means of grub screws.
Page 38: Gassing System
Minifors 2 - Operating manual Setup and Function Gassing System Gases The following gases can be used: Version for microorganisms ■ Oxygen (O ) or Nitrogen (N ■ The basic unit is equipped and configured with two mass flow controllers for controlling the gas flow and, if necessary, the gas mixture.
Page 39: Ph Control
Depending on the variant selected, the measurement system for pH is equipped for digital sensors manufactured by METTLER or HAMILTON. The pH sensors of the Easyferm Plus ARC type have been preconfigured by the INFORS HT device man- ufacturer. Replacement sensors must be configured before use.
Page 40 Minifors 2 - Operating manual Setup and Function Calibration Generally speaking, the following applies: The calibration of a pH sensor is always performed BEFORE the autoclaving (⮫ Chapter 7.1.16.1 ‘Calibrating the pH Sensor’ on page 95). If the pH sensor has already been calibrated exter- nally, the bioreactor will use this data and there is no need for calibration process on the operating panel.
Page 41: Control
Minifors 2 - Operating manual Setup and Function Control Function The oxygen saturation in the (culture) medium is measured by the pO sensor and can be adjusted as follows: increase The concentration of oxygen dissolved in the medium (pO ) can be...
Page 42 Minifors 2 - Operating manual Setup and Function Measurement und Calibration Generally speaking, the following applies: Unlike e.g. pH measure- ments, which are calibrated to absolute measurements, the oxygen measurement is always calibrated to a relative reference point. For this purpose, the calibration is set to 100 % relative oxygen saturation, usually with air at max.
Page 43: Antifoam Control
Minifors 2 - Operating manual Setup and Function Antifoam Control Function Foam hinders the exchange of gas between the medium and the gas phase in the head space. The exit gas filter can become clogged with foam, which causes a pressure build-up in the vessel. This can be prevented by adding antifoam agent.
Page 44: Options
Minifors 2 - Operating manual Options Options Turbidity Measurement 3.1.1 Setup and Function Turbidity measurement can be used to draw conclusions regarding the biomass concentration in the culture. To determine the turbidity in the culture two measurement systems are available:...
Page 45 Minifors 2 - Operating manual Options CAUTION The light emitted by the LEDs on the sensor plate is highly sensitive and can damage the iris or retina. The CGQ BioR sensor plate con- tains an infrared LED that emits high energy radiation in the invisi- ble range.
Page 46: Mounting The Turbidity Sensor
Minifors 2 - Operating manual Options 3.1.2 Mounting the Turbidity Sensor Variant ASD12-N For culture vessels DN 90 and DN 145, ASD12-N sensors can be mounted directly into 12 mm/Pg13.5 ports. For culture vessels with DN 115, a sensor holder is used. For more details on the sensor holder, see ⮫ Chapter 4.7 ‘Sensor Holder’...
Page 47: Exit Gas Analysis
Minifors 2 - Operating manual Options Exit Gas Analysis 3.2.1 Setup and Function Setup and Function To allow the user to draw conclusions regarding the status of the culture while the bioprocess is still underway, the CO and O meas- urements are often taken and analysed in the exit gas flow of the bioreactor.
Page 48 Minifors 2 - Operating manual Options Establishing the Hose Connection The hose connection between the culture vessel (exit gas filter) and the gas sensor must be designed in line with the direction in which the gas flows through the gas sensor: Cut as short a piece as possible off the supplied pressure hose.
Page 49: Redox Measurement
Minifors 2 - Operating manual Options Redox Measurement For measurement of the reduction/oxidation potential (redox) in the medium, a redox sensor can be connected instead of the pO sensor. The prerequisite for this is that the device is equipped for HAMILTON...
Page 50: Balances
Minifors 2 - Operating manual Options Balances The touch screen software allows the connection of one balance to the bioreactor. If more than one balance is to be connected, the connection via the bioprocess software eve is required. ® Balances of the following type are available from the device manufac- turer: Kern DS 30K0.1...
Page 51: Accessories
Minifors 2 - Operating manual Accessories Accessories Accessories in the Scope of Delivery The table below lists all the accessories included in the standard package, divided according to vessel size (TV = total volume) and nominal diameter (DN), inside diameter) as well as version of the device.
Page 52: Cone Plug For Drive Hub
Minifors 2 - Operating manual Accessories Accessories 1.5 l TV / DN 90 3.0 l TV / DN 115 6.0 l TV / DN 145 Starter kit Cone plug for drive hub (in starter set) Cone Plug for Drive Hub...
Page 53: Baffles
Minifors 2 - Operating manual Accessories Baffles The baffles are used to mix the culture in culture vessels for microor- ganisms. They are simply inserted into the glass vessel. Blanking Plugs Blanking plugs are used to seal open ports. There are different blanking plugs for the different types of port.
Page 54: Clamping Adapters And Fastening Screws
Minifors 2 - Operating manual Accessories Clamping Adapters and Fastening Screws Clamping adapters are used when mounting the sparger, the various dip tubes and the antifoam/level sensors. The clamping adapter fixes the built-in-part in place and can be used to adjust its mounting depth.
Page 55: Sensor Holder
Minifors 2 - Operating manual Accessories rvorlagen diverse Sensor Holder Sheath rlagen diverse Grub screw Guide bar Fork Hollow screw Sensor holders are used to adjust the mounting depth of sensors (pH, , etc.) in 12 mm/Pg 13.5 ports. The sensor holder, respectively the sensor must be fitted with an O-ring for mounting.
Page 56 Minifors 2 - Operating manual Accessories Addition Port Adapter Ø 7.5 mm Data Value Unit Inside diameter 2 mm Hose connection outside diam- 4 mm eter Mounting depth 17 mm Addition port adapters protrude as far as the head space of the ■...
Page 57: Septum Collar
Minifors 2 - Operating manual Accessories Septum Collar The septum collar with inside thread is used to inoculate the culture in combination with the syringe, injection needle and septum (⮫ Chapter 4.21 ‘Inoculation Accessories and Tools’ on page 70). The septum collar is used to hold the septum in place in the 12 mm/Pg13.5 port.
Page 58: Immersion Pocket For Temperature Sensor (Pt100)
Minifors 2 - Operating manual Accessories Dip Tube, Straight, Ø 6 mm Data Value Unit Inside diameter 3 mm Hose connection outside diam- 4 mm eter The dip tube does not reach as far as the bottom of the vessel.
Page 59: Exit Gas Cooler
Minifors 2 - Operating manual Accessories verse piervorlagen diverse Kopiervorlagen diverse Kopiervorlagen diverse Kopiervorlagen diverse 4.12 Exit Gas Cooler Overview en diverse Kopiervorlagen diverse derrahmen opiervorlage Bilderrahmen Kopiervorlagen diverse Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen diverse e Bilderrahmen Kopiervorlage Bilderrahmen...
Page 60 Minifors 2 - Operating manual Accessories Function The exit gas cooler dries the exit gas through condensation, thus preventing the exit gas filter from becoming clogged with moisture. At the same time, it also prevents liquid loss in the culture medium.
Page 61: Cold Finger
Minifors 2 - Operating manual Kopiervorlagen diverse Accessories Kopiervorlagen diverse Kopiervorlagen diverse Kopiervorlagen diverse 4.13 Cold Finger Kopiervorlagen diverse Kopiervorlagen diverse Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen en diverse Kopiervorlagen diverse Kopiervorlagen diverse ge Bilderrahmen...
Page 62: Reagent Bottles
Minifors 2 - Operating manual Kopiervorlagen diverse Accessories Kopiervorlagen diverse Kopiervorlagen diverse 4.14 Reagent Bottles Kopiervorlagen diverse Kopiervorlagen diverse Kopiervorlage Bilderrahmen Kopiervorlagen diverse Kopiervorlagen diverse Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen Screw cap Hose connector plate...
Page 63: Sampling System Super Safe Sampler
Minifors 2 - Operating manual Accessories 4.15 Sampling System Super Safe Sampler Basically different systems and also individual components are avail- able for sampling. This operating manual describes the operation and handling of the aseptic sampling system Super Safe Sampler com- bined with a dip tube.
Page 64 Minifors 2 - Operating manual Accessories Principle of Function The sample valve on the side arm of the T-piece opens by putting the Luer connector of the syringe into the valve and closes by removing the syringe. No further handling is necessary.
Page 65: Pump Heads
Minifors 2 - Operating manual Accessories Practical Tips for the Use of the Super Sterility of the culture vessel is ensured at all times without the pos- Safe Sampler sible measures mentioned below. The use of a sterile syringe and sterile caps is only necessary if the sample has to be processed under sterile conditions.
Page 66: Vessel Holder
Minifors 2 - Operating manual Accessories 4.17 Vessel Holder Standard en diverse e Bilderrahmen Reagent bottle holder Pump holder The vessel holder for all vessel sizes has two holding devices for a total of four reagent bottles and one holder for the four pump heads.
Page 67: Sterile Filters
Minifors 2 - Operating manual Accessories 4.18 Sterile Filters Sterile filters are used to protect against contamination in both the gassing line and the exit gas line . All the sterile filters in the scope of supply are autoclavable, disposable filters with PTFE diaphragms.
Page 68 Minifors 2 - Operating manual Accessories Figure Diameter Marking Reten- Application tion rate 50 mm green 0.2 µm Version for cell cultures: exit gas 25 mm without 0.2 µm Super Safe Sampler 25 mm without 0.45 µm Reagent bottles (pressure equalisation) Page 68 of 234 20.12.2023...
Page 69: Hoses And Accessories
Minifors 2 - Operating manual Accessories 4.19 Hoses and Accessories Hose type Ø mm Application Pressure hose, fibreglass-woven 6 x 11.9 Water and gas connections (on-site) Pressure hose, fibreglass-woven 6 x 10 Exit gas filter attachment (on exit gas cooler)
Page 70: O-Rings And Gaskets
Minifors 2 - Operating manual Accessories 4.20 O-Rings and Gaskets Designation Ø mm Application O-ring, EPDM 3.53 x 94.84 Top plate gasket, culture vessel, DN 90 O-ring, EPDM 3.53 x 120.24 Top plate gasket, culture vessel, DN 115 O-ring, EPDM 3.53 x 148.8...
Page 71: Starter Set
Minifors 2 - Operating manual Accessories 4.22 Starter Set Each device package comes with a starter kit with a variety of hoses, attachments, inoculation accessories and tools. A detailed contents list is included in each starter kit. 4.23 Service Sets Service sets with O-rings, gaskets, sterile filters, hoses, etc.
Page 72: Transport And Storage
Minifors 2 - Operating manual Transport and Storage Transport and Storage The following specifications are based on transport and storage of an unpacked device at the provider’s site. Transport WARNING Improper transport, use of incorrect auxiliary tools and careless han- dling of the device may lead to injuries and significant damage to property.
Page 73: Installation And Commissioning
Minifors 2 - Operating manual Installation and Commissioning Installation and Commissioning WARNING Faulty installation may lead to dangerous situations or severe loss of property. Follow the installation and commissioning instructions in this oper- ating manual precisely. Operating Conditions at the Installation Location...
Page 74: Power Supply
Minifors 2 - Operating manual Installation and Commissioning 6.3.1 Power Supply Connection Conditions To avoid dangers due to electrical current, the in-house mains connec- tion must meet the following requirements: Constant power supply ■ In addition to that, ensure the following: The voltage values of the device match those of the local mains ■...
Page 75: Gas Supply
Minifors 2 - Operating manual Installation and Commissioning Connection To connect the device to the in-house water supply and drainage, proceed as follows: Cut the required quantity of the supplied pressure hose (Ø = 6 x 11.9 mm). Position the pressure hoses on the appropriately marked hose nozzles on the device.
Page 76: Exit Gas
Minifors 2 - Operating manual Installation and Commissioning Position the pressure hoses on the appropriately marked hose nozzles on the basic unit. Connect the hoses to the in-house gas supply. Secure hoses with hose clamps to prevent slipping. Check to ensure that hoses neither have kinks nor are able to kink and that connections and hoses do not have any leaks.
Page 77: Test Run
Minifors 2 - Operating manual Installation and Commissioning Test Run To become familiar with the basic functions of the device before the first cultivation, a short test run can be executed. The test run comprises: Temperature control (cooling/heating) ■ Stirring ■...
Page 78 Minifors 2 - Operating manual Installation and Commissioning Screw the exit gas cooler into the port on the vessel top plate port. The exit gas cooler is equipped with a new exit gas filter ex-factory. Connect the exit gas cooler to pre-fitted hoses on the basic unit;...
Page 79 Minifors 2 - Operating manual Installation and Commissioning Cooling To activate the cooling system, proceed as follows: On the operating panel, set a low setpoint for the Temperature parameter, e.g. 10 °C, to activate the water supply to the tem- perature control system.
Page 80 Minifors 2 - Operating manual Installation and Commissioning Heating and Adjusting Temperature Precondition: batch is running with temperature control switched on and stirrer running. To test the heating and adjust the temperature, proceed as follows: CAUTION Risk of minor burns if the heated thermal block and thermal...
Page 81 Minifors 2 - Operating manual Installation and Commissioning End of Test After all parameter setpoints have been reached, the test can end here. The inoculation that now takes place during normal operation is not relevant to the test run. Proceed as follows: On the operating panel, press Inoculate and then Stop Batch to stop the batch (process).
Page 82: Before Cultivation
Minifors 2 - Operating manual Before Cultivation Before Cultivation The following chapters describe all the preparatory work before starting the cultivation process. This essentially comprises: Preparing the culture vessel: ■ – Checking the gaskets (O-rings) on accessories and on the cul- ture vessel –...
Page 83: Mounting The Impellers
Minifors 2 - Operating manual Before Cultivation Check the O-ring for sealing the top plate for damage and to ensure that it is positioned correctly in the groove on the vessel flange. Ensure that every built-in-part is equipped with an intact O- ring: Check that the O-rings are correctly positioned and are undam- aged.
Page 84: Mounting Dip Tubes And Spargers
Minifors 2 - Operating manual Before Cultivation Rushton impeller (version for microorganisms) Pitched blade impeller (version for cell cultures) verse 7.1.3 Mounting Dip Tubes and Spargers Straight spargers and dip tubes can be mounted from the outside in the vessel top plate. Curved spargers and dip tubes can only be mounted from the inside to the vessel top plate.
Page 85: Inserting The Vessel Into The Vessel Holder
Minifors 2 - Operating manual Before Cultivation Set the desired mounting depth, align the sparger. Tighten the slotted screw. 7.1.4 Inserting the Vessel into the Vessel Holder To insert the glass vessel into the vessel holder, proceed as follows: Place the flange onto the ring of the vessel holder: The two opposing recesses on the flange fit with the bolt on the ring.
Page 86: Inserting The Baffles
Minifors 2 - Operating manual Before Cultivation Insert the vessel carefully. 7.1.5 Inserting the Baffles Culture vessels for microorganisms are provided with baffles. To insert them into the glass vessel, proceed with caution. 7.1.6 Moistening/Filling the Culture Vessel If the culture vessel is to be autoclaved with the medium, the vessel can be filled before the top plate is put in position and the additional built-in-parts are mounted.
Page 87: Mounting The Top Plate
Minifors 2 - Operating manual Before Cultivation Development of steam is not possible when auto- claving an empty and dry culture vessel. Successful sterilisation is not guaranteed. Ensure that there is liquid in the culture vessel (approx. 10 mL of water per litre of total volume).
Page 88: Mounting The Blanking Plugs
Minifors 2 - Operating manual Before Cultivation 7.1.8 Mounting the Blanking Plugs Ø 10 mm Ports Insert the blanking plug with O-ring into all unused ports. Fix with a fastening screw. Ø 12 mm / Pg13.5 Ports Insert the O-ring and blanking plug into all unused ports.
Page 89: Mounting Addition Port Adapters
Minifors 2 - Operating manual Before Cultivation 7.1.9 Mounting Addition Port Adapters Insert the addition port adapters with O-rings into the four 7.5 mm ports. Fix them with the fastening screw. 7.1.10 Mounting the Feed Needles The procedure for mounting one or more feed needles instead of addition port adapters is the same as for the mounting of the addi- tion port adapters (⮫ Chapter 7.1.9 ‘Mounting Addition Port Adapters’...
Page 90: Mounting The Immersion Pocket For Temperature
Minifors 2 - Operating manual Before Cultivation 7.1.11 Mounting the Immersion Pocket for Temperature Sensor (Pt100) Insert the immersion pocket with the O-ring into the 10 mm port. Fix it with the fastening screw. 7.1.12 Equipping a Port with Septum Collar and Septum for Inoculation For later inoculation with a syringe, the 12 mm/Pg13.5 port in the top...
Page 91 Minifors 2 - Operating manual Before Cultivation Insert the septum into the port. Screw the septum collar into the port by hand. Insert the blanking plug equipped with an O-ring into the septum collar and screw it tight by hand.
Page 92: Preparing The Dip Tube / Addition Port Adapter For Inoculation
Minifors 2 - Operating manual Before Cultivation 7.1.13 Preparing the Dip Tube / Addition Port Adapter for Inoculation If later inoculation is to be carried out by means of a dip tube or addition port adapter, proceed as follows: Mount the dip tube with the clamping adapter or addition port adapter in the port.
Page 93: Mounting The Cold Finger
Minifors 2 - Operating manual Before Cultivation Attach the coupling nut and tighten it by hand in clockwise direction. Equip the exit gas pipe with a piece of pressure hose (D = 6 x 10 mm) and a clean, dry exit gas filter. To do this, plug the inlet side (INLET) with the green marking in the piece of hose.
Page 94: Preparing The Sensors
Minifors 2 - Operating manual Before Cultivation 7.1.16 Preparing the Sensors All sensors that come into contact with the medium are mounted before autoclaving and are autoclaved together with the culture vessel. Note the following about all sensors: Mount all sensors by hand – do not use any tools! ■...
Page 95: Calibrating The Ph Sensor
Minifors 2 - Operating manual Before Cultivation 7.1.16.1 Calibrating the pH Sensor Calibration of a pH sensor must always be carried out before auto- claving. Proceed as follows: Connect the sensor cable (⮫ Chapter 7.3.10 ‘Connecting the pH Sensor’ on page 115).
Page 96: Mounting Sensors With Sensor Holder
Minifors 2 - Operating manual Before Cultivation 7.1.16.3 Mounting Sensors with Sensor Holder For the mounting of a sensor in a 12 mm/Pg13.5 port for culture vessels with a nominal width of 115, a sensor holder must be used. To do so,...
Page 97 Minifors 2 - Operating manual Before Cultivation Insert the sensor into the hollow screw with the thread pointing in the downward direction. Fit the fork of the guide bar into the groove of the hollow screw. Push the hollow screw and the guide bar together upwards and insert the guide bar into the hole of the support guide.
Page 98: Mounting The Antifoam Sensor
Minifors 2 - Operating manual Before Cultivation 7.1.16.4 Mounting the Antifoam Sensor Please note the following points for mounting: The antifoam sensor is equipped with transparent insulation that ■ must be intact, as otherwise a continuous signal "Foam/liquid detected" may be generated.
Page 99: Preparing The Sampling System Super Safe Sampler
Minifors 2 - Operating manual Before Cultivation 7.1.17 Preparing the Sampling System Super Safe Sampler The following figures are for general purposes of com- prehension. To prepare the sampling system Super Safe Sampler for autoclaving, proceed as follows: Attach the hose of the valve assembly to the dip tube.
Page 100: Mounting The Sparger Hose And The Inlet Air Filter
Minifors 2 - Operating manual Before Cultivation Turn the sterile filter carefully by hand in clockwise direction. �� This ensures that the non-return valve/sterile filter screw con- nection is tight. Cover the valve assembly loosely with aluminium foil. Clamp off the hose on the dip tube.
Page 101: Space Gassing
Minifors 2 - Operating manual Before Cultivation Fit the inlet air filter to the hose piece, fit it in the direction of the air flow to the hose end; the nozzle with the red marking remains exposed: Filter Ø = 37 mm: 1.5 L culture vessel for microorganisms and ■...
Page 102: Preparing The Reagent Bottles, Pumps And Hoses
Minifors 2 - Operating manual Before Cultivation 7.1.20 Preparing the Reagent Bottles, Pumps and Hoses When delivered with the device, the reagent bottles are connected to the pump heads. They are equipped with filters for pressure equali- sation and with hoses of the correct length.
Page 103 Minifors 2 - Operating manual Before Cultivation Depending on the application: fill the reagent bottle and reclose NOTICE Use of highly corrosive reagents, such as hydrochloric acid HCl, leads to damage to components made of stainless steel such as e.g. built-in-parts or the top plate.
Page 104 Minifors 2 - Operating manual Before Cultivation Connction between Pump and Cul- ture Vessel Fit silicone hoses for base, acid and feed to addition port adapters and/or feed needles and secure them with cable ties Attach the silicone hose of the antifoam pump to the mounted antifoam sensor in the culture vessel and secure it with a cable tie.
Page 105: Sterile Hose Connections
Minifors 2 - Operating manual Before Cultivation 7.1.21 Sterile Hose Connections If additional vessels are needed and these can only be connected to the culture vessel after autoclaving, such as vessels for the inoculum or bottles for sampling etc., rapid couplings (male/female), sterile con- nectors or –...
Page 106: Fitting The Cone Plug For Drive Hub
Minifors 2 - Operating manual Before Cultivation Place the mounting plate with the pump heads onto the pump holder on the vessel holder. 7.1.24 Fitting the Cone Plug for Drive Hub To prevent the penetration of condensation water into the dive hub during autoclaving, the cone plug provided in the starter set must be fitted.
Page 107: Autoclaving The Culture Vessel
Minifors 2 - Operating manual Before Cultivation Autoclaving the Culture Vessel 7.2.1 Checklist Before Autoclaving Check and ensure the following items before autoclaving: Kopiervorlagen diverse Culture Vessel Task Further information Kopiervorlagen diverse All necessary O-rings are fitted. ⮫ Kapitel 7.1.1, Seite 82 Kopiervorlagen diverse All unused ports are closed with blanking plugs.
Page 108: Autoclaving
Minifors 2 - Operating manual Before Cultivation Kopiervorlagen diverse Sparger, Head Space Gassing, Exit Gas Cooler Task Further information Kopiervorlagen diverse The sparger is equipped with a hose and an inlet air filter. ⮫ Kapitel 7.1.18, Seite 100 Kopiervorlagen diverse Version for cell cultures: addition port adapter for head space gas- ⮫ Kapitel 7.1.19, Seite 101...
Page 109 Minifors 2 - Operating manual Before Cultivation Adhere to the following: Never autoclave the culture vessel dry (⮫ Chapter 7.1.6 ‘Mois- ■ tening/Filling the Culture Vessel’ on page 86). If necessary, pump off any remaining water after autoclaving by ■ means of the dip tube.
Page 110: Connecting The Culture Vessel And Preparing The Cultivation
Minifors 2 - Operating manual Before Cultivation Connecting the Culture Vessel and Preparing the Cultivation As soon as the culture vessel with the accessories has cooled suffi- ciently, it can be hung up within the basic unit and the various cable and hose connections between the basic unit and the culture vessel can be established.
Page 111: Connecting The Gassing
Minifors 2 - Operating manual Before Cultivation WARNING When using heavily corrosive reagents (acids and bases), it is partic- ularly important only to use suitable and undamaged hoses. They must also be securely fastened. Furthermore, the exit gas filter must not be blocked. This ensures that no pressure builds up and no reagent escapes due to burst hoses.
Page 112: Connecting The Exit Gas Cooler
Minifors 2 - Operating manual Before Cultivation 7.3.4 Connecting the Exit Gas Cooler To connect the exit gas cooler to the basic unit, proceed as follows: Remove the aluminium foil from the exit gas filter. Connect the hoses according to the symbols on the basic unit: Insert the rapid coupling of the water outlet hose onto the ■...
Page 113: Filling The Culture Vessel
Minifors 2 - Operating manual Before Cultivation Place the motor onto the drive hub with the groove aligned with the pin on the drive hub. �� The motor is held in its position. 7.3.7 Filling the Culture Vessel Depending on the application, the vessel can be filled after auto- claving.
Page 114: Connecting The Temperature Sensor (Pt100)
Minifors 2 - Operating manual Before Cultivation 7.3.8 Connecting the Temperature Sensor (Pt100) The temperature sensor is not in direct contact with the medium. Insert the sensor into the immersion pocket in the vessel top plate as far as it will go.
Page 115: Connecting The Ph Sensor
Minifors 2 - Operating manual Before Cultivation Insert the black banana plug into the ground connection in the top plate. 7.3.10 Connecting the pH Sensor n diverse The sensor and cable connections of the pH sensors differ depending on the pH measurement system used.
Page 116: Calibrating The Po
Minifors 2 - Operating manual Before Cultivation n diverse METTLER digital, type InPro6860i Sensor head connection (a) Cable socket (b) gen diverse Bilderrahmen HAMILTON digital, type Visiferm DO ARC Sensor head connection (a) Cable socket (b) ge Bilderrahmen 7.3.12 Calibrating the pO...
Page 117: Cultivation
Minifors 2 - Operating manual Cultivation Cultivation The following sections describe the work typically performed during a cultivation. This essentially comprises: Preparing the medium (starting a batch) ■ Sampling ■ Inoculation ■ Harvest ■ (Stopping the batch), if necessary emptying the vessel ■...
Page 118 Minifors 2 - Operating manual Cultivation Using SAMPLE NOW on the operating panel, the sampling can be logged in the electronic logbook and assigned a sample ID (⮫ Chapter 9.1.5 ‘SAMPLE NOW’ on page 132). The method of sampling can vary due to the different analyses carried out by the operator.
Page 119 Minifors 2 - Operating manual Cultivation Screw open the Luer-Lock syringe on the sample valve. Pull back the syringe plunger to remove the desired sampling volume. If the dip tube was rinsed with air, air is sucked in first. Remove it as follows: Unscrew the syringe from the valve assembly.
Page 120 Minifors 2 - Operating manual Cultivation Insert the syringe onto the hose at the sterile filter and push air through. The remaining liquid in the hose and in the dip tube is pushed back into the vessel. Remove the syringe from the sterile filter to fill it with air again.
Page 121: Inoculation
Minifors 2 - Operating manual Cultivation Inoculation Requirements Check and ensure the following before inoculation: Medium has been filled. ■ Heat-labile, separately sterilised substances have been added. ■ The reagent bottles are connected with the pumps and the cul- ■...
Page 122: Inoculation Using Dip Tube / Addition Port Adapter
Minifors 2 - Operating manual Cultivation 8.3.2 Inoculation Using Dip Tube / Addition Port Adapter Fill the inoculum under sterile conditions into the prepared con- tainer. Create a sterile hose connection with the dip tube/addition port adapter. Transfer the desired volume of inoculum into the culture vessel.
Page 123: Emptying The Culture Vessel
Minifors 2 - Operating manual Cultivation Switch off all parameters at the operating panel and stop the batch (process) at the operating panel. Always stop the running batch (process) on the operating panel. If it is stopped by pressing the power switch, it is akin to a power inter- ruption.
Page 124: Emptying The Reagent Hoses
Minifors 2 - Operating manual Cultivation Emptying the Reagent Hoses Before autoclaving the culture vessel with accessories, all reagent hoses must be completely emptied using the corresponding pump. This can either be done manually or time-controlled at the operating panel.
Page 125: Autoclaving The Culture Vessel After Cultivation
Minifors 2 - Operating manual Cultivation Autoclaving the Culture Vessel After Cultivation Depending on internal regulations, the culture vessel is autoclaved with all accessories after emptying and before cleaning. In this case, observe and comply with the same safety instructions as for auto- claving before cultivation.
Page 126 Minifors 2 - Operating manual Cultivation Fit the cone plug into the opening of the drive hub. NOTICE Risk of loss of property du penetration of condensation water into the drive hub! Always autoclave the culture vessel with the cone plug fitted to the drive hub! Open the pump cover.
Page 127: Operation
Minifors 2 - Operating manual Operation Kopiervorlagen diverse iverse Kopiervorlagen diverse Operation Screen Areas, Menu Navigation and Control Elements 9.1.1 Overview Kopiervorlage Bilderrahmen lderrahmen Kopiervorlage Bilderrahmen Kopiervorlagen diverse Kopiervorlagen diverse Kopiervorlagen diverse Left side with selection menus for system settings...
Page 128: Main Screen
Minifors 2 - Operating manual Operation Header Status Display The following symbols and displays are in the header: Settings symbol: to switch between menu selection for system ■ settings and parameter groups Display of the current time ■ Display for connected USB stick ■...
Page 129 Minifors 2 - Operating manual Operation MAIN with actual values in the VALUE Parameter Groups Example: parameter group SETPOINT column. column and input fields for setpoints in the Active Menus/Parameter Group(s) All menus and parameter groups can be selected by pressing them.
Page 130: Edit View
Minifors 2 - Operating manual Operation Instead of using the arrow buttons, swiping move- ments to the left, right, upwards or downwards on the screen can also change the display. 9.1.3 EDIT VIEW EDIT VIEW opens a menu with all available parameters. Here, up to...
Page 131: Start Batch / Inoculate / Stop Batch
Minifors 2 - Operating manual Operation 9.1.4 START BATCH / INOCULATE / STOP BATCH Pressing the START BATCH button starts the preparation phase for the batch (bioprocess). The controller is activated. The current param- eter settings are simultaneously logged in a log file, and recording of the actual values begins.
Page 132: Sample Now
Minifors 2 - Operating manual Operation The button now changes function to STOP BATCH. After pressing STOP BATCH, a dialogue appears asking for confirma- tion that the batch is to be stopped, as well as the notice that doing so switches all parameters off.
Page 133 Minifors 2 - Operating manual Operation SAMPLE NOW generates consecutive numbers for all samples and logs them with the batch time since inoculation as the time stamp. This means that an information dialogue appears and displays how Batch Time…. ), long the batch has been running since inoculation ( Sample Number….
Page 134: Menus For System Settings
Minifors 2 - Operating manual Operation Menus for System Settings 9.2.1 Overview There are seven menus for system settings, of which five are available for the operator. VESSEL TYPE: selection of the culture vessel used ■ APPEARANCE: display settings, including language and date/time ■...
Page 135: Vessel Type - Selecting A Culture Vessel
Minifors 2 - Operating manual Operation 9.2.2 VESSEL TYPE – Selecting a Culture Vessel VESSEL TYPE menu. There are The culture vessel used is set in the three culture vessel sizes. NOTICE With the selection of the culture vessel used, the permitted limit values and control settings for the corresponding vessel size are configured in the background.
Page 136: Appearance - Display Settings
Minifors 2 - Operating manual Operation 9.2.3 APPEARANCE – Display Settings APPEARANCE menu. A variety of display settings can be made in the LANGUAGE To select the display language. The desired display language can be selected using the drop-down list.
Page 137 Minifors 2 - Operating manual Operation After pressing an info button, a dialogue appears containing basic information on the selected parameter, example to the left: parameter STIRRER . SYNC DATE AND TIME VIA NTP If this function is activated, the touchscreen synchronizes its date and time with a network time server (NTP) present and configured in the network.
Page 138: Network Settings
Minifors 2 - Operating manual Operation 9.2.4 NETWORK SETTINGS The network connection of the bioreactor is configured in the NETWORK SETTINGS menu. If the bioreactor is to be integrated into an existing network, the network specifications are to be fol- lowed and the corresponding settings are to be used.
Page 139: Eve Communication - Communication Settings
Minifors 2 - Operating manual Operation SUBNET MASK Displays the subnet mask address during automatic configuration Auto (DHCP) ), or can be used to enter the subnet mask for manual Manual ). configuration ( The network connection can be used to connect the device with the bioprocess platform software eve ®...
Page 140: Usb Data Export And Import From A Usb Stick
Minifors 2 - Operating manual Operation 9.2.6 USB Data Export and Import from a USB Stick USB menu, a USB stick connected with the device’s USB port In the can be used to import or export data. EXPORT DATA TO USB Opens the menu for data export.
Page 141 Minifors 2 - Operating manual Operation EXPORT CONFIG: to export a backup of the current device config- ■ uration as zip file to the USB stick, which can be imported again by LOAD CONFIGURATION FROM USB . CANCEL: to cancel and leave the menu without changes.
Page 142 Minifors 2 - Operating manual Operation yyyy_mm_dd_hh_ii_ss_Measurement.csv Log of the actual values of all parameters during the batch in CSV format. The recording interval is 1 min. If higher precision is required, SCADA software, e.g. the bioprocess platform software eve , can ®...
Page 143: System Info - System Information
Minifors 2 - Operating manual Operation 9.2.7 SYSTEM INFO – System Information SYSTEM INFO menu displays some important system information. HMI SERIAL NUMBER : serial number of the operating panel ■ VERSION : current firmware version & version of the device (for ■...
Page 144: Parameter - Parameter Groups
Minifors 2 - Operating manual Operation Parameter – Parameter Groups 9.3.1 Overview On the main screen, up to eight parameters can be simultaneously monitored and controlled. The parameters are divided into five param- eter groups: FAVORITES: allows you to compile up to eight parameters from ■...
Page 145: Parameters - Displays And Functions
Minifors 2 - Operating manual Operation 9.3.2 Parameters – Displays and Functions Columns Irrespective of the selected parameter group, every parameter menu has the same three columns. PARAMETER : display of the parameter ■ VALUE : display of the actual value of the parameter ■...
Page 146: Setpoint - Setting The Setpoint
Minifors 2 - Operating manual Operation Editing Opens the editor menu with the various settings for the selected parameter. Not all parameters have an editor menu. E.g. cascades can be set here, PID settings can be adjusted, parame-ter alarms can be switched on or off or the pump functions can be selected.
Page 147: Parameter Alarms
Minifors 2 - Operating manual Operation Inadmissible Setpoints If an inadmissible setpoint is entered, an error message is generated that prompts a correct entry within the permissible parameter set- points. Stirrer setpoint entry Example (version for microorganisms): The is too high. Make a new entry within the permissible values of 0 to 1600 min 9.3.4...
Page 148 Minifors 2 - Operating manual Operation In the parameter group that contains the parameters in question, a ■ number on a red background appears. This indicates the number of existing parameter alarms. The parameter in question is displayed with a red bar and an actual ■...
Page 149: Cascades
Minifors 2 - Operating manual Operation Parameter Alarm limit Value Unit Flow mL min Flow mL min Flow mL min Air Headspace mL min Version for microorganisms Version for cell cultures 9.3.5 Cascades Cascades can be configured for some parameters. A cascade can be used to assign a parameter to another parameter as an actuator.
Page 150: Main Parameter Group
Minifors 2 - Operating manual Operation Configuration The cascades can be configured using the editor menu of the param- eter. The procedure is described in the parameter description for the parameters for which this is possible. MAIN Parameter Group MAIN parameter group contains all parameters that are available...
Page 151: Stirrer
Minifors 2 - Operating manual Operation 9.4.2 Stirrer Measures and controls the speed of the stirrer. Rotation speed depends on factors such as the size of the motor, vessel volume, culture viscosity, number and kind of impellers etc. Stirring speed is often used in a cascade for pO control.
Page 152 Minifors 2 - Operating manual Operation Additional choice for version for cell cultures: Flow – Base : pH control takes place by adding base and CO ■ (instead of liquid acid). Only CO Flow : pH control only takes place by adding CO (instead ■...
Page 153 Minifors 2 - Operating manual Operation Settings The settings for the cascade are made in the editor menu of the parameter. CASCADE menu item three drop-down lists are available. They At the contain all the parameters available for configuring a serial cascade of up to 3 stages for pO control.
Page 154 Minifors 2 - Operating manual Operation The selected setting is represented visually. The following example Stirrer (stirrer speed). shows the setting with control using The PID settings can be adjusted here as required or, if necessary, can be reset to factory settings using RESET PID (⮫ Chapter 9.9 ‘PID Controller –...
Page 155: Total Flow
Minifors 2 - Operating manual Operation 9.4.5 Total Flow Air Flow ) Measures and controls the sum of the volume flows of air ( and one or two connected gas(es): Version for microorganisms: gassing with a second gas (oxygen OR ■...
Page 156: Foam
Minifors 2 - Operating manual Operation FEATURE , has the following The only menu item available here, options: Only Air : air is exclusively used, with no addition of a second gas. ■ Total Flow corre- The gas mixture always contains 21 % oxygen. The Air Flow .
Page 157 Minifors 2 - Operating manual Operation Alternatively, the antifoam sensor can be configured as a level sensor Pump3 pumps medium/liquid into the culture vessel until the so that desired fill level has been reached, respectively the sensor detects liquid. The direction of rotation of the pump cannot be changed.
Page 158: Extended Parameter Group
Minifors 2 - Operating manual Operation DOSE TIME : duration (in seconds) of the addition of antifoam ■ Pump3 agent, respectively culture medium by WAIT TIME ■ Antifoam : duration (in seconds) after the addition of – Feature antifoam agent to reduce foam before more antifoam agent is added.
Page 159: Flow Parameters
Balances must be configured with the following values: Baudrate 9600, 8 bits, no parity, 2 stop bits. For a list of compatible balances or help with the con- nection, please contact your local INFORS HT service partner. 9.5.2 Flow Parameters...
Page 160 Minifors 2 - Operating manual Operation Air Flow Regardless of the existing device version and configuration of the gassing system, the setpoint for the air volume flow is ALWAYS set in TotalFlow parameter. A setpoint can NEVER be set in the Air Flow parameter, as the oxygen concentration is ALWAYS controlled by the GasMix parameter, even if only air is used.
Page 161: Turbidity (Optional)
Minifors 2 - Operating manual Operation If the parameter is configured for pH control, it is automatically accepted in the pH parameter as an actuator in a cascade. In this case, the setpoint value can no longer be edited in the parameter.
Page 162 Minifors 2 - Operating manual Operation DEVICE and MODE offer the following options: The two menu items DEVICE : Setting the type of parameter, choice between NONE , ■ PUMP or CUSTOM (customized) Settings here are only relevant, if working with eve ®...
Page 163: Exit Gas Parameter Group
Minifors 2 - Operating manual Operation EXIT GAS Parameter Group EXIT GAS parameter group contains the parameters for the optional exit gas analysis (⮫ Chapter 3.2 ‘Exit Gas Analysis’ on page 47). Details on technical data, usage and maintenance requirements for the gas sensors can be found in separate documentation provided by the manufacturer.
Page 164: Pumps Parameter Group
Minifors 2 - Operating manual Operation PUMPS Parameter Group 9.7.1 Overview PUMPS parameter group, the delivery rate of the pumps can In the be set or monitored and the function mode of the pumps can be configured. In addition, the pump hoses can be manually filled or emptied by pressing and holding FILL or EMPTY.
Page 165: Configuring The Pumps
Minifors 2 - Operating manual Operation Default Settings The pumps are configured as follows ex-factory: Pump Version for microorganisms Version for cell cultures Pump1 Acid Feed addition of acid, digital addition of nutrient solution, analogue ■ ■ pH parameter controlled by the controlled by the user ■...
Page 166 Minifors 2 - Operating manual Operation TUBE TYPE To select the pump hose used. The following pump hoses are available: 0.5 mm, 1.0 mm (standard) or 2.5 mm. On the basis of the selected hose diameter, the pumped volume can be estimated and used for the display of the totalled DISPLAY COUNT UNIT ).
Page 167: Pump1
Minifors 2 - Operating manual Operation 9.7.3 Pump1 Pump1 can be configured for the Acid function or Feed . Acid : digital operating mode, is used in pH control to add acid. ■ Feed : analogue (continuous) operating mode, can be used for addi- ■...
Page 168: Pump4
Minifors 2 - Operating manual Operation The direction of rotation of the pump cannot be changed. If, however, culture medium is to be pumped out of the culture vessel as soon as the sensor detects liquid, this can be done by selecting...
Page 169: Auto Fill/Empty - Automatically Filling/Emptying Pump Hoses
Minifors 2 - Operating manual Operation Dose : analogue (continuous) operating mode, is used for the addi- ■ tion of a defined weight of nutrient solution. The desired feed rate in grams is entered by pressing START DOSE. The key pad appears for typing in the desired dosing weight.
Page 170 Minifors 2 - Operating manual Operation For each pump, an individual filling duration as well as an individual emptying duration can be defined. Pressing FILL or EMPTY starts the filling or emptying procedure for the corresponding pump and the pump hose is filled or emptied for the set filling or emptying duration.
Page 171: Calibrating The Sensors
Minifors 2 - Operating manual Operation Calibrating the Sensors Sensors for measuring the pH, pO and turbidity (ASD12-N) are usually calibrated before each cultivation. Depending on the sensor and measurement system, either a 2-point calibration should be used, or a 1-point calibration and a zero point adjustment is sufficient.
Page 172 Minifors 2 - Operating manual Operation Carefully remove the cap with storage solution from the pH sensor and rinse the sensor with distilled water, do not rub it. NOTICE Wiping or rubbing the pH sensor after flushing can generate an electrostatic charge. This can greatly increase the response time and generate incorrect measurements.
Page 173 Minifors 2 - Operating manual Operation Select 2-point calibration. �� The menu display changes to the first calibration point and shows the following: Left-hand side: guides step-by-step (1 – 5) through the ■ calibration of the first reference value. Right-hand side: ■...
Page 174 Minifors 2 - Operating manual Operation Press CALIBRATE POINT 1 to start calibration (step 4). �� CONFIRM NOW slowly turns orange and indicates the ideal waiting time until a stable measured value is reached. If the measured value is assumed to be...
Page 175: Ph Sensor Product Calibration
Minifors 2 - Operating manual Operation 9.8.2 pH Sensor Product Calibration General Information It is possible to adjust the calibration curve to the current process conditions using product calibration. This could be necessary if there is a possibility of drift of the displayed pH during a long-term cultivation, for example.
Page 176 Minifors 2 - Operating manual Operation Take a sample from the process (in the culture vessel). There are two possible approaches: Variant A: confirm the sampling (generate a time stamp), ■ carry out a laboratory measurement of the pH value for the sample, enter the measured value and carry out product calibration.
Page 177: Calibrating The Po
Minifors 2 - Operating manual Operation Variant B Proceed as follows: Press CONFIRM. �� The date and time of the sampling are now displayed. Leave the calibration menu using OK and carry out a laboratory measurement of the pH value for the sample at a later time of your choosing.
Page 178 Minifors 2 - Operating manual Operation Call up the calibration menu of the parameter. �� The menu display changes after a short initialization phase: Header: date and time of the last calibration ■ 1 POINT CALIBRATION: to select 1-point calibration ■...
Page 179 Minifors 2 - Operating manual Operation Left-hand side: guides step-by-step (1 – 5) through the ■ calibration of the first reference value. Right-hand side: ■ – Drop-down list for selection of the reference value. If the connected sensor allows the use of different reference values or an automatic detection of the ref- erence value ("AUTO"), it can be selected.
Page 180: Calibrating The Turbidity Sensor
Minifors 2 - Operating manual Operation If the calibration process fails, an error message is displayed with a corresponding message. Restart the calibration in this case. If the calibration is successful, the menu display changes automati- cally to calibrate the second calibration point. The step-by-step guide (steps 6 - 10) through the calibration remains the same as for the first point (steps 1 - 5).
Page 181 Minifors 2 - Operating manual Operation Header: date of the last calibration ■ Left-hand side: calibration sequence (2 steps) ■ Right-hand side: measured value and CALIBRATE: to cali- ■ brate the zero point. Wait until the measured value is stable.
Page 182: Pid Controller - Basic Principle
Minifors 2 - Operating manual Operation PID Controller – Basic Principle For some parameters, PID controllers (Proportional Integral Derivative controller) are used. 9.9.1 Table with Settings for the PID Controller Setting Description P (Prop. Term) Proportional term: The greater the difference between setpoint and actual value, the greater the controller output.
Page 183: Tips For Readjusting A Pid Controller
Minifors 2 - Operating manual Operation 9.9.2 Tips for Readjusting a PID Controller To readjust the PID controller, proceed as follows: Start by setting the proportional factor when readjusting a PID controller. Choose the proportional bandwidth as large as pos- sible.
Page 184: Alarms - Equipment Alarm Menu
Equipment errors: if equipment errors occur repeatedly or cannot ■ be resolved, inform an authorised INFORS HT service partner. Equipment Alarm s only available when there are open or uncon- firmed alarms. Otherwise the alarm symbol (a red exclamation mark highlighted in white on a red background) is hidden in the lower screen edge.
Page 185: Switching Off The Device
Minifors 2 - Operating manual Operation DESCRIPTION : describes the type of alarm. ■ STATE : status display of the alarm, open or resolved . ■ Open . – Open alarms are displayed in red and with the word –...
Page 186: 10 Cleaning And Maintenance
Minifors 2 - Operating manual Cleaning and Maintenance 10 Cleaning and Maintenance The following chapters contain general descriptions regarding the cleaning of the culture vessel and its accessories, and how these can be stored. In addition, the chapter contains a maintenance plan and corre- sponding descriptions for the procedures to be performed by the operator.
Page 187: Cleaning The Culture Vessel - Routine Cleaning
Minifors 2 - Operating manual Cleaning and Maintenance 10.2 Cleaning the Culture Vessel - Routine Cleaning NOTICE Household washing-up liquid and soap (in particular cream soaps) can collect in glass pores and impair later cultivations. Never clean culture vessels and accessories with household soap and use special cleaning agent (for industrial and lab use) in the lab washer.
Page 188: Removing The Vessel Top Plate And Accessories
Minifors 2 - Operating manual Cleaning and Maintenance Let the motor cool down. When the motor has cooled down: Uncouple the motor. Remove the top plate and carefully place it so that it does not lie on top of components.
Page 189 Minifors 2 - Operating manual Cleaning and Maintenance Remove the lid by hand. If necessary, wet the lid slightly with water to facilitate loosening of the lid. Remove the silicone baffle from the exit gas cooler. Clean the individual parts of the exit gas cooler (⮫ Chapter 10.4 ‘Cleaning and Storing Individual Parts’...
Page 190: Removing The Sensors
Minifors 2 - Operating manual Cleaning and Maintenance 10.3.2 Removing the Sensors 10 mm ports (antifoam/level) Loosen and remove the fastening screw beside the sensor by hand. Loosen the slotted screw at the clamping adapter. Carefully remove the sensor from the clamping adapter.
Page 191: Removing Blanking Plugs
Minifors 2 - Operating manual Cleaning and Maintenance Ensure that the inlet air filter is clean, dry and not blocked. If this is not the case, dispose of it. If the filter for pressure equalisation and the corresponding hoses have been used several times, ensure that the filters are always dry and clean.
Page 192: Removing Addition Port Adapters, Feed Needle And Temperature Sensor Immersion Pocket
Minifors 2 - Operating manual Cleaning and Maintenance 10.3.6 Removing Addition Port Adapters, Feed Needle and Temperature Sensor Immersion Pocket Loosen and remove the fastening screw between the addition port adapters and/or feed needle(s) as well as beside the immer- sion pocket by hand.
Page 193: Removing The Sparger And The Dip Tube(S)
Minifors 2 - Operating manual Cleaning and Maintenance 10.3.8 Removing the Sparger and the Dip Tube(s) Straight spargers and dip tubes can be removed from the outside of the vessel top plate. Curved spargers and dip tubes can only be removed from the inside of the vessel top plate.
Page 194: Cleaning And Storing Individual Parts
Minifors 2 - Operating manual Cleaning and Maintenance 10.4 Cleaning and Storing Individual Parts The procedure described here applies to the following individual parts: Vessel ■ Exit gas cooler ■ Accessories such as blanking plugs, spargers, dip tubes, addition ■...
Page 195: Cleaning The Sensors
Minifors 2 - Operating manual Cleaning and Maintenance 10.5 Cleaning the Sensors Apart from antifoam and level sensor, all sensors are cleaned and maintained according to the descriptions of the sensor manufacturer. Clean the sensors according to the sensor manufacturer guide- lines.
Page 196: Cleaning The Basic Unit And Operating Panel
Minifors 2 - Operating manual Cleaning and Maintenance If the test record requires that the culture is killed off after cultivation by autoclaving the culture vessel, the valves of the sampling system may become stuck due to reside of the culture solution. In such a situa-...
Page 197: 10.10 Decalcifying The Device
Minifors 2 - Operating manual Cleaning and Maintenance Interval Maintenance work Personnel Prior to each use Check hose lines and connections. Operator Check cables for damage and kinks. Operator Check all O-rings and gaskets and replace if necessary. Operator Check the integrity of all glass parts (vessel, reagent Operator bottles) and replace if necessary.
Page 198 Minifors 2 - Operating manual Cleaning and Maintenance NOTICE Amidosulfonic acid can crystallise in case of overdosage and cause loss of property! When preparing the decalcifying liquid, observe and follow the man- ufacturer’s instructions for correct dosage and application! Proceed as follows for decalcifying: Mount the exit gas cooler into the port of the vessel top plate and connect to the basic unit.
Page 199: 11 Rectifying Faults
Minifors 2 - Operating manual Rectifying Faults 11 Rectifying Faults The following chapter describes possible reasons for faults and how to resolve them. Reduce the service intervals in correspondence with the actual loads if faults become increasingly common. Contact the manu- facturer or licensed dealer in case of faults that cannot be resolved by following the notices below.
Page 200: Faults Drive System
Minifors 2 - Operating manual Rectifying Faults 11.2 Faults Drive System Fault description Cause Remedy Personnel Motor does not start. Motor is not properly con- Switch off the device. Operator nected. Check cable connec- tions and connect cor- rectly as nec- essary.
Page 201: Faults Temperature Control System
Minifors 2 - Operating manual Rectifying Faults 11.3 Faults Temperature Control System Fault description Cause Remedy Personnel No temperature control. Temperature control is not acti- Activate the Temperature Operator vated. parameter. Stirrer Stirrer is not activated and/or Activate the Operator setpoint = 0.
Page 202: Faults Gassing System
Minifors 2 - Operating manual Rectifying Faults 11.4 Faults Gassing System Fault description Cause Remedy Personnel No gassing / air bubbles The on-site gas supply has been Stop the batch Operator in the culture vessel. interrupted. (process). Check the on-site...
Page 203 Minifors 2 - Operating manual Rectifying Faults Fault description Cause Remedy Personnel Overpressure alarm Exit gas filter is blocked, over- Replace the exit gas filter Operator pressure high is dis- pressure sensor switches gassing under sterile conditions. played, the desired gas off for 10 s.
Page 204: Faults Ph Control
Minifors 2 - Operating manual Rectifying Faults 11.5 Faults pH Control Fault description Cause Remedy Personnel No display or incorrect Sensor cable is not con- Connect the sensor cable Operator display of pH. Digital nected or not properly con- correctly.
Page 205 Minifors 2 - Operating manual Rectifying Faults Fault description Cause Remedy Personnel Acid , No pH control. The (base/acid) pump does Check the pump ( Operator not operate properly. Base ) functionality using the operating panel (switch on/ switch off).
Page 206: Faults Po
Minifors 2 - Operating manual Rectifying Faults 11.6 Faults pO Control Fault description Cause Remedy Personnel No display or incorrect Sensor cable is not con- Connect the sensor cable cor- Operator display of pO Digital nected or not properly con- rectly.
Page 207: Faults Antifoam/Level And Pump
Minifors 2 - Operating manual Rectifying Faults 11.7 Faults Antifoam/Level and Pump Fault description Cause Remedy Personnel Foam/medium is not Sensor is not properly con- Check connections and Operator detected. nected. connect properly as nec- essary. Foam/medium is Sheathing of antifoam sensor is...
Page 208: Faults Feed And Pump
Minifors 2 - Operating manual Rectifying Faults 11.8 Faults Feed and Pump Fault description Cause Remedy Personnel No addition or inad- Feed parameter (pump) is Activate the Feed param- Operator equate addition of not activated. eter (pump). nutrient solution. Feed parameter (pump) setpoint...
Page 209: Interferences Turbidity Measurement
Minifors 2 - Operating manual Rectifying Faults 11.9 Interferences Turbidity Measurement Fault description Cause Remedy Personnel Displayed measured Sensor cable is twisted or Check the connec- Operator value is not plausible/ kinked or not properly con- tion of the sensor unusual.
Page 210: Replacing Device Fuses
Minifors 2 - Operating manual Rectifying Faults 11.10 Replacing Device Fuses The device fuses may only be replaced by fuses of the same rating. For information on the requirements regarding fuses, see ⮫ Chapter 13.3.1 ‘ Electrical Con- nection and Power Values’ on page 217.
Page 211: 12 Disassembly And Disposal
Minifors 2 - Operating manual Disassembly and Disposal 12 Disassembly and Disposal The device must be disassembled and disposed of in an environmen- tally-friendly manner if it is not in use anymore. If the device is to be returned to the manufacturer for...
Page 212 Minifors 2 - Operating manual Disassembly and Disposal For disposal, the system units must be disassembled and dismantled into individual material groups. These materials must be disposed of according to the applicable national and local legislation. Local author- ities or specialist disposal firms can provide information regarding environmentally acceptable disposal.
Page 213: 13 Technical Data
Minifors 2 - Operating manual Technical Data 13 Technical Data 13.1 Dimensions Front View Device Kopiervorlagen diverse Kopiervorlagen diverse Kopiervorlagen diverse Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen Pos. Value 565 mm Both device versions 631 mm 740 mm Culture vessels DN 115 & DN 145 for microor-...
Page 214 Minifors 2 - Operating manual Technical Data Kopiervorlagen diverse piervorlagen diverse Top View Device Kopiervorlagen diverse Kopiervorlagen diverse Kopiervorlage Bilderrahmen piervorlage Bilderrahmen Kopiervorlage Bilderrahmen Pos. Value Kopiervorlage Bilderrahmen 415 mm 375 mm 400 mm 455 mm Page 214 of 234...
Page 215 Minifors 2 - Operating manual Technical Data Culture Vessel The dimension drawings show a fully equipped culture vessel ready for autoclaving. Dimensions in mm 20.12.2023 Page 215 of 234...
Page 216 Minifors 2 - Operating manual Technical Data The two dimension drawings show an equipped culture vessel in the compact vessel holder available in addition to the standard vessel holder (only for culture vessel 1.5 L TV). Dimensions in mm Page 216 of 234...
Page 217: Weight
Minifors 2 - Operating manual Technical Data 13.2 Weight Data Value Unit Basic unit 23.5 ± 0.5 kg Culture vessel DN 90 6.0 ± 0.5 kg Culture vessel DN 115 7.0 ± 0.5 kg Culture vessel DN 145 9.0 ± 0.5 kg Equipped culture vessel, without medium, with vessel holder.
Page 218: Water
Minifors 2 - Operating manual Technical Data 13.3.2 Water Water Inlet Basic Unit Data Value Unit Connection pressure 2 ± 1 bar Hose nozzle connection, nom- 6 mm inal width Min. flow temperature 10 °C Data Value Water quality CaCO concentration 0 mmol L to 1.5 mmol L...
Page 219: Process Gas
Minifors 2 - Operating manual Technical Data 13.3.3 Process Gas Data Value Unit Constant connection pressure 2 ± 0.5 Hose nozzle connection, nom- inal width Data Value General gas quality Dry, clean and free of oil and dust Recommended compressed air...
Page 220 Minifors 2 - Operating manual Technical Data Materials Data Value Glass vessel Borosilicate glass Top plate and built-in parts AISI 316L, electropolished O-rings (in contact with EPDM product) Exception: impellers in culture vessel 1.5 L / DN 90 for microorgan- isms are made of PEEK.
Page 221: Stirrer
Minifors 2 - Operating manual Technical Data 13.4.3 Stirrer Drive General Data Value Drive Shaft with mechanical seal Direction of rotation of drive Counter-clockwise = to left shaft Bearing Outside vessel, in drive hub Motor type DC, brushless Drive Version for Microorganisms...
Page 222 Minifors 2 - Operating manual Technical Data Drive Version for Cell Cultures Data Value Unit Nominal power of motor 74 W Range of rotation speed 24 to 600 min Accuracy measurement and ± 2 control at 24 to 300 min Accuracy measurement and ±...
Page 223 Minifors 2 - Operating manual Technical Data Impellers for Cell Cultures Data Value Type Pitched blade impeller with 3 blades, angled 45° Material AISI 316L, electropolished Flow direction blades Standard: downwards, Option: upwards Dimensions and Quantity Impellers Vessel Quantity 6.0 L TV / DN 145...
Page 224: Temperature Control System
Minifors 2 - Operating manual Technical Data Impeller Mounting Heights ex-factory Vessel Pitched blade impeller Rushton impellers 6.0 L TV / DN 145 16 mm 4.5 mm 137 mm 3.0 L TV DN 115 17 mm 6.0 mm 110 mm 1.5 L TV / DN 90...
Page 225: Gassing System
Minifors 2 - Operating manual Technical Data Temperature Range and Accuracy Data Value Unit Measurement range 0 to 145 °C Control range Min. flow tem- °C perature + 5 to Accuracy measurement ± 0.1 °C Accuracy control ± 0.2 °C at 20 °C to 60 °C...
Page 226 Minifors 2 - Operating manual Technical Data Version for Cell Cultures Data Value Unit Gas entry Sparger, head space Specific gas flow rate, calculated 2000 mL min for the max. working volume Gas(ses) Flow control Accuracy MFC 5 Mass flow control- ±...
Page 227: Ph Control
Measurement principle sensor Potential measurement against reference Measurement range pH 0 to 14 pH sensors type Easyferm Plus ARC are preconfigured by the device manufacturer INFORS HT. Replacement sensors must be configured before use. Measurement System METTLER Data Value Sensor type...
Page 228: Control
Measurement principle sensor Optical Measurement range 8 ppb to 60 % O saturation The pO sensors are preconfigured by the device man- ufacturer INFORS HT. Replacement sensors must be configured before use. 13.4.8 Antifoam Control Data Value Sensor Conductive with dosing needle...
Page 229: Pumps
Minifors 2 - Operating manual Technical Data 13.4.9 Pumps Data Value Type Peristaltic Quantity Control Analogue or digital Control (operating modes): Analogue = continuous operation with variable speed ■ Digital = OFF/ON operation with fixed speed ■ Hoses Standard Option 1 Option 2 Inside Ø...
Page 230: Exit Gas Analysis
Minifors 2 - Operating manual Technical Data Variant CGQ BioR Data Value Measurement modes Green (521 nm) or Infrared (940 nm) Measurement range 0 to 1000 13.5.2 Exit Gas Analysis For Aerobic Bioprocesses Sensor type selection Measurement Measurement range vol. % range vol. %...
Page 231: Operating Conditions
Minifors 2 - Operating manual Technical Data 13.6 Operating Conditions Data Value Unit Ambient temperature 5 to 40 °C Relative humidity, non-con- 20 to 90 % densing Altitude operating location Max. 2000 m.a.s.l Pollution degree as per EN 61010-1 Min. distance from walls, ceilings...
Page 232: 14 Eu Declaration Of Conformity
Minifors 2 - Operating manual EU Declaration of Conformity 14 EU Declaration of Conformity Page 232 of 234 20.12.2023...
Page 233: Eg-Konformitätserklärung
Anschrift Address Adresse Konformitätsbeauftragter Representative for conformity Responsable de la conformité Bottmingen, 16. Nov. 2021 M. Heuschkel Ort, Datum Chief Technology Officer Place, date Lieu, date Doc. No. CER-CE-0009 Version Page 1 of 1 Company Confidential - © INFORS HT...
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