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Infors HT Techfors-S Operating Manual

Pilot scale bioreactor
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Operating manual
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Summary of Contents for Infors HT Techfors-S

  • Page 1 Techfors-S Operating manual We bring life to your laboratory.
  • Page 2 Techfors-S – Rel. 2.0 Pilot scale bioreactor SW: 3.5 Doc-ID: D011, 1, en_GB – Original Art. 79258 More information about the product is INFORS HT available online at: Headoffice, Switzerland http://www.infors-ht.com/en/techfors-s Rittergasse 27 CH-4103 Bottmingen T +41 (0)61 425 77 00 info@infors-ht.com...

  • Page 3: Supplemental Directives

    Techfors-S - Operating manual Supplemental directives Supplemental directives About this Manual This manual enables the safe and efficient handling of the device. All the information and instructions in this operating manual comply with the current standards, legal regulations and the latest technological developments.
  • Page 4 Intended Use, Incorrect Use and Misuse............. Qualified Personnel......................... 1.3.1 Operator..........................1.3.2 Technician........................1.3.3 INFORS HT Service Technician or Licensed Dealer..Unauthorized Persons......................Responsibility of the Provider..................Residual Risks..........................Warning Symbols on the Device................Declaration of Decontamination................2 Basic Unit................................23 Overview Basic Unit.......................

  • Page 5: Table Of Contents

    Techfors-S - Operating manual Table of contents 2.5 Harvest/Sample Valve (Bottom Valve)............42 2.5.1 Setup and Function..................... 42 2.5.2 Using the Harvest/Sample Valve............44 2.6 Vessel Pressure Display (Manometer)............. 48 2.6.1 Setup and Function..................... 48 2.6.2 Mounting the Manometer................. 48 2.7 Stirrer................................
  • Page 6 Techfors-S - Operating manual Table of contents 2.14 Safety Valves..........................2.14.1 General Information................... 2.14.2 Safety Valve Temperature Control Circuit......2.14.3 Safety Valve Vessel.................... 2.14.4 Mounting the Vessel Safety Valve ..........2.15 Identification of the Device..................85 2.15.1 Identification Plate....................85 2.15.2 Plant Identification Plate................
  • Page 7 Techfors-S - Operating manual Table of contents 3.13 pCO Measurement........................ 107 3.14 Redox Measurement......................108 3.15 Permissive Measurement....................109 3.16 Pumps..............................109 3.17 Balances..............................110 3.18 Mobile CIP Unit TechCIP....................110 4 Accessories, Material and Hoses..................111 Overview of the Accessories..................
  • Page 8 Techfors-S - Operating manual Table of contents 6.3 Requirements for the Mains Connection............ 130 6.4 Requirements for Connections and Interfaces........130 6.5 Safety Valves..........................133 7 Cultivation..............................134 Preparing the Bioreactor....................134 7.2 In Situ Sterilization........................137 Preparing the Medium......................138 7.4 Sampling...............................
  • Page 9 Techfors-S - Operating manual Table of contents 8.3.2.4 Login/Logout – Logging in to/out of the System......................165 8.3.2.5 Change Own Password – Changing the (Own) Password................165 8.3.2.6 New User – Adding a New User........ 166 8.3.2.7 Edit User – Changing User Settings....... 167 8.3.2.8...
  • Page 10 Techfors-S - Operating manual Table of contents 8.6 Parameter Options........................190 8.6.1 Overview of Settings Menu..............190 8.6.2 Setpoint..........................191 8.6.2.1 Menu Content.................. 191 8.6.2.2 Setting Setpoints, Activating and Deacti- vating Parameters................ 192 8.6.2.3 Setting Alarm Limits and Critical Values..194 8.6.3 Calibrate..........................
  • Page 11 Techfors-S - Operating manual Table of contents 8.8 Pumps..............................221 8.8.1 Control and Settings..................221 8.8.2 Calibrating the Pumps..................221 8.8.3 Resetting the Pump Counter to Zero........... 223 8.8.4 Filling and Emptying the Pump Hoses......... 224 8.9 Processes............................. 226 8.9.1 General Information...................
  • Page 12 Techfors-S - Operating manual Table of contents 8.9.6.4 Executing the Process............237 8.9.6.5 Aborting the Process............... 238 8.9.7 Bioreactor Operation – Cultivation..........239 8.9.7.1 Process Sequence................ 239 8.9.7.2 Process Configuration............. 239 8.9.7.3 Before Starting the Process..........240 8.9.7.4 Starting the Process..............240 8.9.7.5...
  • Page 13 Techfors-S - Operating manual Table of contents 12 Technical Data............................262 12.1 Dimensions............................262 12.2 Connections and Connection Values............... 263 12.3 Specifications of the Basic Unit................265 12.3.1 Instrumentation Cabinet................265 12.3.2 Operating Panel....................... 265 12.3.3 Vessel........................... 265 12.3.4 Temperature Control System..............267 12.3.5 Stirrer............................
  • Page 14 Techfors-S - Operating manual Table of contents 12.6 Operating Materials........................ 282 12.7 Emissions.............................. 283 Page 14 of 284 08.01.2025...

  • Page 15: Safety And Responsibility

    Techfors-S - Operating manual Safety and Responsibility Safety and Responsibility This chapter contains general information on safety when using the device. In the remaining chapters, warning messages are used only to highlight particular hazards directly arising from the actions being described.

  • Page 16: Intended Use, Incorrect Use And Misuse

    Safety and Responsibility Intended Use, Incorrect Use and Misuse Intended Use The pilot scale bioreactor Techfors-S that can be sterilized in situ has been specially developed for the cultivation of microorganisms for research and development in a biotechnology laboratory and exclusively for the use of fluids of group 2 according to Article 13 of the Pressure Equipment Directive 2014/68/EU.
  • Page 17 Techfors-S - Operating manual Safety and Responsibility Qualified Personnel 1.3.1 Operator The operator operates the device in the context of the intended use. Only persons who have been trained for working in a biotechnology laboratory can be considered for the role of operator. These include,...

  • Page 18: Unauthorized Persons

    Techfors-S - Operating manual Safety and Responsibility 1.3.3 INFORS HT Service Technician or Licensed Dealer Certain work may only be performed by the manufacturer's skilled per- sonnel or by skilled personnel authorized by a licensed dealer. Other persons are not authorized to perform this work.
  • Page 19 Techfors-S - Operating manual Safety and Responsibility Electric Current The device is operated electronically. There is an immediate risk of fatal injury if contact is made with live parts. The following points must be observed in order to avoid the risk of fatal injury: In case of damage to insulation, disconnect the device from the ■...
  • Page 20 Techfors-S - Operating manual Safety and Responsibility Corrosive or Toxic Substances The use or production of corrosive or toxic substances entails a signifi- cant health risk. As such, special measures must be taken to protect personnel. Safely discharge contaminated condensate.

  • Page 21: Warning Symbols On The Device

    Techfors-S - Operating manual Safety and Responsibility Environmental Hazards Inappropriate handling of environmentally hazardous substances, especially where disposal is involved, may lead to severe environ- mental damage. Contaminated liquids must be disposed of in an environmentally ■ suitable way. Accessories and Spare Parts...

  • Page 22: Declaration Of Decontamination

    Techfors-S - Operating manual Safety and Responsibility Declaration of Decontamination When returning the device for repair, disassembly or disposal, a legally compliant declaration of decontamination is required for the safety of all involved and due to legal requirements. The following must be...

  • Page 23: Basic Unit

    Techfors-S - Operating manual Basic Unit Basic Unit Kopiervorlagen diverse Overview Basic Unit Kopiervorlagen diverse Kopiervorlagen diverse Kopiervorlage Bilderrahmen Kopiervorlagen diverse Kopiervorlagen diverse Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen Kopiervorlagen diverse Kopiervorlagen diverse Kopiervorlagen diverse Kopiervorlagen diverse Kopiervorlage Bilderrahmen...

  • Page 24: Additional Information

    Techfors-S - Operating manual Basic Unit Pos. Designation Additional Information Operating panel ⮫ Chapter 2.2.2, page 25 Main switch ⮫ Chapter 2.2.1, page 25 Pumps ⮫ Chapter 2.3.1, page 28 Gassing system ⮫ Chapter 2.9.1, page 59 Harvest/sample valve (bottom valve) ⮫ Chapter 2.5.1, page 42 Vessel ⮫ Chapter 2.4.1, page 31...

  • Page 25: Operating Panel

    Techfors-S - Operating manual Basic Unit Operating and Display Elements 2.2.1 Main Switch The main switch is located on the top right-hand corner on the front of the instrumentation cabinet. To switch on the device, turn the main switch clockwise (90 ■...
  • Page 26 Kopiervorlagen diverse Techfors-S - Operating manual Basic Unit Kopiervorlagen diverse Monitor Buttons on the Operating Panel Kopiervorlage Bilderrahmen Kopiervorlagen diverse Kopiervorlage Bilderrahmen Kopiervorlagen diverse Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen ON/OFF key DARK key: setting the screen illumination to darker LIGHT key: setting the screen illumination to brighter...
  • Page 27 Techfors-S - Operating manual Basic Unit Operating Panel Connections Kopiervorlagen diverse Kopiervorlagen diverse Kopiervorlagen diverse Kopiervorlagen diverse Kopiervorlagen diverse Kopiervorlagen diverse USB2.0 x 2: for backups and service purposes USB2.0 x 2: reserve Ethernet Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen...
  • Page 28 Techfors-S - Operating manual Basic Unit Pumps 2.3.1 Setup and Function Function By default, the lower part of the instrumentation cabinet features four peristaltic pumps with hinged cover. The pumps are driven by stepper motors and run clockwise in automatic operation. The pumps are...
  • Page 29 Techfors-S - Operating manual Basic Unit Release the clamp. �� The pump hose is now secured by the left clamp. Thread the pump hose around the pump head and push in gently. 08.01.2025 Page 29 of 284...
  • Page 30 Techfors-S - Operating manual Basic Unit Slowly turn the pump head clockwise with one hand and sup- port the pump hose guide with the other hand. CAUTION Danger of pinching fingers! If the pump is operated electrically (rocker switch) when the pump hose is inserted, this can lead to pinched fingers and damage to the pump hose.
  • Page 31 Vessel identification plate (⮫ Chapter 2.15.3, page Vessel jacket The pilot scale bioreactor Techfors-S is available in three vessel sizes: 15, 30 and 42 liter total volume. The only difference between the ves- sels is the number ports and the type of harvest/sample valve (bottom valve).
  • Page 32 Techfors-S - Operating manual Kopiervorlagen diverse Basic Unit Kopiervorlagen diverse Vessel Top Plate Kopiervorlagen diverse Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen diverse Kopiervorlagen diverse Kopiervorlage Bilderrahmen Drive hub for motor Valve 02.16.01 19 mm port (8 or 9 x) for built-in-parts/accessories 10 mm port with immersion pocket for the temper-...
  • Page 33 Techfors-S - Operating manual Basic Unit O-ring (top plate seal) ervorlagen diverse Stirrer shaft Rushton impeller (2 or 3 x) Baffles (4 x) ervorlagen diverse Ring sparger Spacer, demountable (4 x) ervorlage Bilderrahmen ervorlagen diverse ervorlage Bilderrahmen iervorlage Bilderrahmen...
  • Page 34 Techfors-S - Operating manual Basic Unit Ingold Nozzles The Ingold nozzles are easily accessible on the bottom of the front of the vessel. The number of Ingold nozzles varies depending on the size of the vessel, see table. Vessel TV...
  • Page 35 Techfors-S - Operating manual Basic Unit 2.4.2 Removing and Mounting the Vessel Top Plate General Information The vessel top plate can be lifted either manually or by using a suit- able lifting device. This is a two person job in either case.
  • Page 36 Techfors-S - Operating manual Basic Unit Carefully and vertically lift the vessel top plate from the vessel using the two handles. In doing so, ensure that baffle, sparger and stirrer shaft do not touch the inside of the vessel. NOTICE If built-in-parts hit the inside of the vessel, this can result in micro scratches.
  • Page 37 Techfors-S - Operating manual Basic Unit Attach the washers. Attach the cap nuts (M10) and tighten them crossways using the wrench (size 17 mm). 2.4.3 Checking Impellers, Baffles and the Top Plate Seal NOTICE Manipulations on the mechanical seal can damage it.
  • Page 38 Techfors-S - Operating manual Basic Unit Stirrer Shaft and Impeller Ensure that the impellers (2) on the stirrer shaft are set to the desired height and sit tightly. If necessary, loosen the two grub screws (M5x6) per impeller (3) on the impellers, correctly position the impellers and re-tighten the grub screws.
  • Page 39 Techfors-S - Operating manual Basic Unit Screw the blanking plug with the O-ring into the septum collar by hand. Use the hexagon socket spanner to tighten the blanking plug. 2.4.5 Closing Unused Ports All unused ports and nozzles in the vessel top plate and on the vessel must be sealed with blanking plugs before sterilization.
  • Page 40 Techfors-S - Operating manual Basic Unit Ingold Nozzle Blanking Plug Insert the blanking plug with O-ring into the Ingold nozzle. Tighten the blanking plug by hand. 2.4.6 Cleaning the Vessel After completion of cultivation and, depending on the user's require- ments, subsequent in situ sterilization for decontamination, the vessel must be cleaned.
  • Page 41 Techfors-S - Operating manual Basic Unit 2.4.7 Cleaning the Vessel Top Plate Depending on the application, it might be necessary to separately clean vessel top plate. To thoroughly clean the vessel top plate, pro- ceed as follows: Lift off the vessel top plate, swing it off, lower it and place it on a suitable surface with the inside of the top plate facing upwards (for details, see ⮫ Chapter 2.4.2 ‘Removing and...

  • Page 42: Harvest/Sample Valve (Bottom Valve)

    Techfors-S - Operating manual Basic Unit Harvest/Sample Valve (Bottom Valve) 2.5.1 Setup and Function 05.12.01 is installed Overview The combined harvest/sample valve (bottom valve) in the connection nozzle on the vessel bottom ex-factory. Also the steam trap and clean steam line (pressure hose) with manual valve Kopiervorlagen diverse 05.10.01 are mounted.
  • Page 43 Techfors-S - Operating manual Basic Unit Harvest/Emptying Valve Type for 15 L and 30 L TV Valve Type for 42 L TV Kopiervorlagen diverse n diverse Kopiervorlagen diverse Kopiervorlage Bilderrahmen For harvest/emptying, the provider’s hose line can be To harvest/empty, a valve insert with a nozzle (a) is...

  • Page 44: Using The Harvest/Sample Valve

    Techfors-S - Operating manual Basic Unit Opening/Closing the Valve gen diverse Kopiervorlagen diverse Valve Type for 15 L and 30 L TV Valve Type for 42 L TV Kopiervorlagen diverse verse age Bilderrahmen Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen Open the valve: turn clockwise (a).
  • Page 45 Techfors-S - Operating manual Basic Unit Unscrew the steam trap counter-clockwise from the needle in the valve. �� The needle is now visible. Place the sample bottle under the needle. Or, if necessary, use the needle to pierce the septum on the sample bottle.
  • Page 46 Techfors-S - Operating manual Basic Unit Harvest: Valve Type for 15 L and 30 L To harvest the culture, proceed as follows: Open the clamp and remove the steam trap with the flat gasket from the connection flange of the harvest/sample valve.
  • Page 47 Techfors-S - Operating manual Basic Unit Harvest: Valve Type for 42 L TV To harvest the culture, proceed as follows: Turn the steam trap counter-clockwise to remove it from the needle in the harvest/sample valve. Loosen the groove nut and remove the valve insert with needle.

  • Page 48: Vessel Pressure Display (Manometer)

    Techfors-S - Operating manual Basic Unit Vessel Pressure Display (Manometer) 2.6.1 Setup and Function The maximum permissible vessel pressure is 3.0 bar. Manometer 08.30.01 indicates the pressure in the vessel in a range from 0 bar to 4.0 bar. The manometer has two different measuring scales. The outer scale corresponds to the pressure at a vessel temperature of 25 °C.

  • Page 49: Stirrer

    Techfors-S - Operating manual Basic Unit orlagen diverse Stirrer 2.7.1 Setup and Function Overview Drive hub Mechanical seal Stirrer shaft Impeller orlagen diverse The stirrer shaft is driven from above and turns clockwise (right/top view). The stirrer shaft is screwed onto the drive hub in the top plate orlage Bilderrahmen and sealed using a single mechanical seal.
  • Page 50 Techfors-S - Operating manual Basic Unit Impeller Depending on the vessel size, two (15 L TV) or three (30 L and 42 L TV) Rushton impellers are affixed to the stirrer shaft using grub screws. Motor An air-cooled servo motor is used as drive. The motor is coupled by placing it on the drive hub on the vessel top plate.
  • Page 51 Replacing the mechanical seal If there is a significant loss of glycerine, an INFORS HT service technician must check whether the mechanical seal needs to be replaced.

  • Page 52: Coupling And Uncoupling The Motor

    Techfors-S - Operating manual Basic Unit 2.7.2 Coupling and Uncoupling the Motor Safety Notes CAUTION The motor is heavy! Work in pairs when coupling and uncoupling the motor. CAUTION Touching the motor during operation or during the cool-down phase can cause slight burns.
  • Page 53 Techfors-S - Operating manual Basic Unit Place the motor in the holder of the lifting device on the central column (a) or (if available) into the holder for the vessel top plate (b). orlage Bilderrahmen Coupling the Motor...

  • Page 54: Lubricating The Mechanical Seal

    Techfors-S - Operating manual Basic Unit 2.7.3 Lubricating the Mechanical Seal To ensure lubrication of the mechanical seal, the silicone hose at the bottom of the drive hub of the top plate must always be filled with liquid (glycerine). NOTICE...

  • Page 55: Temperature Control System And Sterilization

    Techfors-S - Operating manual Basic Unit Temperature Control System and Sterilization erse 2.8.1 Setup and Function Kopiervorlagen diverse Overview The temperature control system consists of a heating element and a circulation pump, which ensures circulation of the heating/cooling liquid in the vessel jacket. The figure below shows the temperature...
  • Page 56 Techfors-S - Operating manual Basic Unit Cooling Cooling is effected via tap water or a cooling water system provided by the provider. The water is fed directly into the vessel jacket, or the temperature control circuit. Optional 3-way ball valves on the water inlet and water outlet facilitate manual switching between tap water and chilled water (⮫ Chapter 3.5 ‘Switching between Tap Water/Chilled Water’...
  • Page 57 Techfors-S - Operating manual Basic Unit Pressure Indicator The maximum permissible pressure in the temperature control system 01.30.01 installed on the water outlet of the is 3.0 bar. The manometer vessel jacket indicates the pressure in a range from -1.0 bar to +4.0 bar.

  • Page 58: Mounting The Temperature Sensor

    Techfors-S - Operating manual Basic Unit 2.8.2 Mounting the Temperature Sensor The mounting of the temperature sensor varies depending on the size of the vessel. For vessels with 15 L and 30 L TV, this happens in the vessel top plate. For vessels with 42 L , this happens in an Ingold nozzle.

  • Page 59: Gassing System

    Techfors-S - Operating manual Basic Unit Gassing System 2.9.1 Setup and Function Gases The following gases can be used: Air (Air) ■ Oxygen (O ■ Nitrogen (N ■ Gassing Units Depending on the chosen gassing strategy, the device is fitted and configured with the corresponding gassing units, that is, rotameter, solenoid valve and mass flow controller.
  • Page 60 Techfors-S - Operating manual Basic Unit Kopiervorlagen diverse Gas Connections The gas connections are located on the rear of the device and are Kopiervorlagen diverse labelled with the corresponding gas. Each gas connection has a check valve (a), manometer (b) and a pressure reducing valve (c). The number of connections varies depending on the configuration.
  • Page 61 Techfors-S - Operating manual Basic Unit 02.16.01 Inlet Air Filter and Valve Inlet air filter 02.16.01 Valve A steam sterilizable membrane filter is placed in the gassing line. The filter has two manual twist valves. The condensate hose is connected to the lower twist valve (b).
  • Page 62 Techfors-S - Operating manual Basic Unit Mounting Gassing hose flange Vessel top plate flange The inlet air filter is connected to the flange on the vessel top plate with a clamp (b) and a flat gasket (a). The same applies to the connec- tion of the gassing hose to the inlet air filter.

  • Page 63: Removing And Mounting The Inlet Air Filter

    Techfors-S - Operating manual Basic Unit 2.9.2 Removing and Mounting the Inlet Air Filter Removing the Inlet Air Filter Remove the clamp between the inlet air filter and connection flange on the vessel top plate. Remove the inlet air filter und flat gasket.

  • Page 64: Exit Gas System

    Techfors-S - Operating manual Basic Unit 2.10 Exit Gas System 2.10.1 Setup and Function diverse Overview Any cultivation can increase the pressure inside the vessel through heating or gas production, even without active gassing. Hence, an exit gas line must be available for every cultivation in a bioreactor.
  • Page 65 Techfors-S - Operating manual Basic Unit Exit Gas Filter A steam sterilizable membrane filter is used as the exit gas filter. The filter has two manual twist valves. The condensate hose is connected to the lower twist valve (b). This valve must be open. The upper twist valve (a) is not used and must be closed.

  • Page 66: Exit Gas Filter

    Techfors-S - Operating manual Basic Unit 2.10.2 Removing and Mounting the Exit Gas Cooler with Exit Gas Filter Removing the Exit Gas Cooler with The exit gas cooler and exit gas filter can be removed as a unit. Exit Gas Filter However, the exit gas hose line must be removed.
  • Page 67 Techfors-S - Operating manual Basic Unit Turn over the exit gas cooler and place it in the holder on the vessel mounting. Mounting the Exit Gas Cooler with Exit Gas Filter Remove the exit gas cooler from the holder and turn it around.

  • Page 68: Cleaning The Exit Gas Cooler

    Techfors-S - Operating manual Basic Unit Place the flat gasket and exit gas cooler flush on the flange on the vessel top plate and secure them with a clamp. Place the flat gasket and flange of the exit gas hose flush on the flange of the exit gas filter and affix them with a clamp.
  • Page 69 Techfors-S - Operating manual Basic Unit Open the clamp between the exit gas filter and the exit gas cooler. Remove the clamp and flat gasket and put aside for subsequent mounting. Remove the hose clamps and disconnect the red pressure hoses for water inlet and outlet from the exit gas cooler.

  • Page 70: Ph Control

    METTLER or digital sensors manufactured by HAMILTON. The pH sensors of the Easyferm Plus ARC type have been preconfigured by the INFORS HT device man- ufacturer. Replacement sensors must be configured before use.

  • Page 71: Mounting And Connecting The Ph Sensor

    Techfors-S - Operating manual Basic Unit Mounting The pH sensor is mounted into an Ingold nozzle in the vessel. The sensor’s manufacturer includes suitable insertion housing with the sen- sors. 2.11.2 Mounting and Connecting the pH Sensor Insertion Housing Insertion housing serves as a sensor adapter and also protects the sensor from physical damage.
  • Page 72 Techfors-S - Operating manual Basic Unit METTLER digital, type InPro 3253i with head transmitter M100 Sensor head connection (a) Cable socket (d) Head transmitter M100: push-in fitting for sensor (b) ahmen Head transmitter M100: push-in fitting for cable (c) gen diverse...

  • Page 73: Control

    METTLER or digital sensors manufactured by HAMILTON. Digital pO sensors have been preconfigured by the INFORS HT device manufacturer. Replacement sensors must be configured before use. Measurement system Properties...

  • Page 74: Mounting And Connecting The Po Sensor

    Techfors-S - Operating manual Basic Unit Measurement und Calibration Generally speaking, the following applies: unlike e.g. pH measure- ments, which are calibrated to absolute measurements, the oxygen measurement is always calibrated to a relative reference point. For this purpose, the calibration is set to 100 % relative oxygen saturation, usually with air at max.
  • Page 75 Techfors-S - Operating manual Basic Unit Sensor and Cable Connections The sensor and cable connections of the pO sensors differ depending diverse on the pO measurement system used. METTLER analog, type InPro 6820/25/080 (amperometric, polarographic) diverse Sensor head connection (a)
  • Page 76 Techfors-S - Operating manual Basic Unit Mounting and Connecting the pO Sensor If necessary, insert the sensor into the insertion housing according to the sensor manufacturer’s guidelines. Insert the sensor into the Ingold nozzles and tighten it manually using the coupling nut.

  • Page 77: Antifoam Control

    Techfors-S - Operating manual Basic Unit 2.13 Antifoam Control 2.13.1 Setup and Function Function Foam hinders the exchange of gas between the medium and the gas phase in the head space. The exit gas filter can become clogged with foam, which can cause a pressure build-up in the vessel. Several measures can be taken to prevent this.
  • Page 78 Techfors-S - Operating manual Basic Unit Prior to autoclaving, the antifoam sensor should be set to the approxi- mate mounting depth. It should be set too low rather than too high. Pulling out is possible even during cultivation and bears a significantly lower risk of contamination than pushing in.
  • Page 79 Techfors-S - Operating manual Basic Unit Carefully loosen the hollow screw by hand. Set the antifoam sensor to the desired mounting depth. Ensure that the sensor does not touch the hollow screw. Other- wise, a continuous signal is generated as soon as the sensor is connected.

  • Page 80: Mounting And Connecting The Antifoam Sensor

    Techfors-S - Operating manual Basic Unit 2.13.3 Mounting and Connecting the Antifoam Sensor After autoclaving and after in situ sterilization, proceed with mounting the antifoam sensor as with an inoculation needle. For details, see ⮫ ‘Using Inoculation Needles’ on page 123.
  • Page 81 Techfors-S - Operating manual Basic Unit 2.14 Safety Valves 2.14.1 General Information All installed safety valves have been certified by the German Technical Inspection Association (TÜV). As with all safety-related components, they must be included in a maintenance plan; otherwise their opera- tional safety cannot be guaranteed.
  • Page 82 Techfors-S - Operating manual Basic Unit 2.14.3 Safety Valve Vessel Setup and Function The safety valve mounted on the vessel top plate with lifting device protects the bioreactor vessel (not the vessel jacket) against an inad- missible overpressure. It must be mounted and intact at all times.

  • Page 83: Mounting The Vessel Safety Valve

    Techfors-S - Operating manual Basic Unit gen diverse iervorlagen diverse Overpressure Venting Pipe The safety valve has a connection thread for mounting in the 19 mm port in the vessel top plate. It also has a short overpressure venting pipe.
  • Page 84 Techfors-S - Operating manual Basic Unit Insert the safety valve with O-ring into the 19 mm port and screw it in by hand. Ensure that the provider-side hose or piping is connected to the overpressure venting pipe; connect if necessary. For details, see also ⮫ Chapter 2.14.1 ‘Safety Valves’ on page 81.

  • Page 85: Identification Of The Device

    Techfors-S - Operating manual Basic Unit 2.15 Identification of the Device 2.15.1 Identification Plate Position The identification plate for identifying the device is located on the instrumentation cabinet and on the support. Content The identification plate is designed to allow clear identification of the device.

  • Page 86: Vessel Identification Plate

    Techfors-S - Operating manual Basic Unit 2.15.3 Vessel Identification Plate The vessel identification plate is welded on the outside of the vessel. It contains the following information: Device manufacturer and address ■ Vessel manufacturer and address ■ Factory number/year of manufacture ■...

  • Page 87: Options

    Techfors-S - Operating manual Options Options Overview of the Options The following options are available in addition to the standard device. Designation Additional Information Lateral sample valve ⮫ Chapter 3.2.1, page 88 Resterilizable feed line ⮫ Chapter 3.3.1, page 90 Steam generator ⮫ Chapter 3.4, page 94...

  • Page 88: Sample Valve

    Techfors-S - Operating manual Options Sample Valve 3.2.1 Setup and Function gen diverse 17.13.01 is installed in the Ingold nozzle on the side of the Overview Sample valve vessel. Also the steam trap is affixed to the valve using the condensate...

  • Page 89: Using The Sample Valve

    Techfors-S - Operating manual Options 3.2.2 Using the Sample Valve Safety Notes CAUTION The sample valve gets very hot during sterilization. Depending on the cultivation temperature, it can also get hot during operation. This poses a risk of burns when touching the sample valve.

  • Page 90: Resterilizable Feed Line

    Techfors-S - Operating manual Options Kopiervorlagen diverse Resterilizable Feed Line orlagen diverse 3.3.1 Setup and Function Kopiervorlage Bilderrahmen Kopiervorlagen diverse vorlage Bilderrahmen Kopiervorlagen diverse Kopiervorlagen diverse Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen Clean steam line (pressure hose with rapid cou-...

  • Page 91: Preparing The Resterilizable Feed Line

    Techfors-S - Operating manual Options 3.3.2 Preparing the Resterilizable Feed Line Safety Notes CAUTION The components of the feed line get very hot during sterilization. − After sterilization, let the feed line cool down. − Wear heat-resistant protective gloves when operating the valves of the feed line.
  • Page 92 Techfors-S - Operating manual Options 13.16.01 / 13.16.03 13.16.01 / 13.16.03 with the vessel, proceed as Sterilize Block Valve To sterilize block valve in situ follows: 13.16.01 / 13.16.03 Insert the connection nozzle of the block valve into in a 19 mm port in the vessel top plate and tighten manually.
  • Page 93 Techfors-S - Operating manual Options Sterilizing the Feed Line Once the full sterilization and the sterilization in the autoclave are completed and the components have cooled down: Loosen the clamp and remove it together with the condensate 13.16.01 / 13.16.03 .

  • Page 94: Steam Generator

    Techfors-S - Operating manual Options Steam Generator Function The steam generator is used for the sterilization of the vessel and peripheral devices and is also used for heating, depending on the tem- perature control system chosen. The steam generator is available in two variants with different output.

  • Page 95: Switching Between Tap Water/Chilled Water

    Techfors-S - Operating manual Options Switching between Tap Water/Chilled Water If a cooling water system provided by the operator is available or a recirculating chiller, which is available separately, is used, 3-way ball 01.41.01 (water inlet) and 01.41.02 (water outlet) can be used to valves switch between tap water and chilled water.

  • Page 96: Lifting Device For Vessel Top Plate

    Techfors-S - Operating manual Options Lifting Device for Vessel Top Plate A lifting device is available for lifting and lowering the vessel top plate. This device is mounted on the central column of the basic unit and operated using a rope winch. We recommend using a lifting device in particular for vessels with a total volume of 30 liters and 42 liters.

  • Page 97: Level Detection

    Techfors-S - Operating manual Options Level Detection 3.8.1 Setup and Function Function The level sensor detects liquid in the vessel. As soon as it detects Output of the parameter Level indi- liquid, a signal is generated. The cates "100". agen diverse This signal can be used as required for level control in the vessel in order to keep the working volume in the vessel constant.
  • Page 98 Techfors-S - Operating manual Options NOTICE If the level sensor is fixed too tightly in the clamping adapter, or the mounting depth of the level sensor is changed while the hollow screw on the clamping adaptor is tightened, the transparent insula- tion may be damaged.
  • Page 99 Techfors-S - Operating manual Options Connect the sensor cable with the two banana connectors: Insert the red banana connector into the connector (a) on ■ the top of the sensor. Insert the black banana connector into ground connection ■ (b) in the vessel top plate.

  • Page 100: Pressure Control

    Techfors-S - Operating manual Options Pressure Control gen diverse 3.9.1 Setup and Function 03.11.01 ( exit gas ) Function Pressure control valve 08.31.01 Pressure sensor The pressure in the vessel is measured using a piezo-resistive pressure transmitter and regulated with a pressure control valve in the exit gas line.
  • Page 101 Techfors-S - Operating manual Options Carefully remove the protective cap from the steel membrane of the pressure sensor. NOTICE The steel membrane is very delicate and can be damaged by friction or knocks from hard objects. Carefully insert the pressure sensor with O-ring into the 19 mm port and tighten the hollow screw by hand.

  • Page 102: Weight Measurement Vessel - Load Cell

    Techfors-S - Operating manual Options 3.10 Weight Measurement Vessel – Load Cell Setup and Function Kopiervorlagen diverse Kopiervorlage Bilderrahmen Frame of the weighing system Load cell (3 x) Holder for exit gas cooler The weighing system of the vessel consists of a frame with three load cells.

  • Page 103: Turbidity Measurement

    Techfors-S - Operating manual Options 3.11 Turbidity Measurement 3.11.1 Setup and Function Function The ASD25-N measurement system from the manufacturer Optek is used to determine the turbidity of the culture. The turbidity can be used to draw conclusions regarding the biomass concentration in the culture.

  • Page 104: Mounting And Connecting The Turbidity Sensor

    Techfors-S - Operating manual Options 3.11.2 Mounting and Connecting the Turbidity Sensor Due to the position, length and angle of the Ingold nozzle on the vessel, the mounting position of the turbidity sensor is pre-defined. It prevents a gas connection at the port on the top, and medium can drain from sapphire windows.

  • Page 105: Exit Gas Analysis

    Techfors-S - Operating manual Options 3.12 Exit Gas Analysis Setup and Function To allow the user to draw conclusions regarding the status of the culture while the bioprocess is still underway, the CO and O meas- urements are often taken and analyzed in the exit gas flow of the bioreactor.
  • Page 106 Techfors-S - Operating manual Options During cultivation, exit gas is routed through the gas sensors. During sterilization, it must be discharged directly into the provider’s exit gas line to protect the sensors from moisture. The operator manually 03.41.01 . makes this switch using the 3-way ball valve...

  • Page 107: Pco 2 Measurement

    Techfors-S - Operating manual Options 3.13 Measurement Setup and Function The saturation of dissolved carbon dioxide (CO ) in the culture is measured using a digital CO sensor with integrated temperature sensor. Measurements in hPa are displayed on the corresponding transmitter and also in the touch screen software.

  • Page 108: Redox Measurement

    Techfors-S - Operating manual Options 3.14 Redox Measurement Overview The reduction/oxidation potential (redox) in the medium is measured using the redox sensor. Depending on the variant selected, the measurement system is equipped and configured for analog sensors by the manufacturer MET- TLER or digital sensors by the manufacturer HAMILTON.

  • Page 109: Permissive Measurement

    ® The sensor with the corresponding transmitters must be purchased directly from the manufacturer ABER. INFORS HT offers a connection to the transmitter on the basic unit. Calibration Calibration is performed according to the manufacturer’s guidelines directly on the transmitter.

  • Page 110: Balances

    CIP unit and its operation in general are described in detail in a separate operating manual. To be able to clean the bioreactor with the CIP unit, an adjusted Techfors-S vessel design is required. In this case, the vessel has two additional tri-clamp ports with blanking plugs. Page 110 of 284...

  • Page 111: Accessories, Material And Hoses

    Techfors-S - Operating manual Accessories, Material and Hoses Accessories, Material and Hoses Overview of the Accessories By default (column S), the device is supplied with some accessories and material. Additional accessories are optionally available (column Designation Additional Information Starter set ⮫ Chapter 4.2, page 111...

  • Page 112: Connection Set

    Techfors-S - Operating manual Accessories, Material and Hoses Connection Set The connection set contains the same hose and attachment material for all vessel sizes. Accessories Ø mm Pump hose (Marprene/Bioprene) 3.2 x 6.4 Connection reagent bottle to peristaltic pump Pressure hose 8.0 x 14.5...

  • Page 113: Reagent Bottles

    Techfors-S - Operating manual Accessories, Material and Hoses Kopiervorlagen diverse Reagent Bottles 4.4.1 Setup and Function Kopiervorlagen diverse Kopiervorlagen diverse Overview Kopiervorlagen diverse Kopiervorlagen diverse Kopiervorlage Bilderrahmen Kopiervorlagen diverse Kopiervorlagen diverse Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen...

  • Page 114: Preparing Reagent Bottles For Cultivation

    Techfors-S - Operating manual Accessories, Material and Hoses Function Reagent bottles are used as containers for reagent and nutrient solu- tion. Connected to the appropriate peristaltic pump and an addition utensil, e.g. an inoculation needle, the liquid is pumped into the vessel.
  • Page 115 Techfors-S - Operating manual Accessories, Material and Hoses Place a long piece of silicone hose on the equilateral connection on the outside of the hose connector plate. Choose the length of the hose so that it reaches from the reagent bottle to the pump on the instrumentation cabinet without tension or sharp kinks.

  • Page 116: Specifications

    Techfors-S - Operating manual Accessories, Material and Hoses 4.4.3 Specifications Volumes/Hoses Size/volume Silicone hose diameter 500 mL 2 x 6 mm 1000 mL 3 x 5 mm 2000 mL 3 x 5 mm 5000 mL 3 x 5 mm 10000 mL...

  • Page 117: Push Valves

    Techfors-S - Operating manual Accessories, Material and Hoses Kopiervorlagen diverse Push Valves 4.5.1 Setup and Function Overview Kopiervorlagen diverse Kopiervorlagen diverse Kopiervorlagen diverse Kopiervorlage Bilderrahmen Kopiervorlagen diverse Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen Hose connector, 1 x or 4 x...

  • Page 118: Preparing Push Valves For Cultivation

    Techfors-S - Operating manual Accessories, Material and Hoses Opening/Closing Push Valves verse Opening the push valve: turn the rotary piston Closing the push valve: turn the rotary piston ■ ■ clockwise. counter-clockwise. The lower part of the rotary piston is pushed out. The The lower part of the rotary piston is retracted.
  • Page 119 Techfors-S - Operating manual Accessories, Material and Hoses Ensure that the push valve is closed, and close it if necessary. Secure all hose connections with cable ties. Lightly cover the filter of the reagent bottle(s) with aluminum foil. Wrap the push valve in aluminum foil.

  • Page 120: Specifications

    Techfors-S - Operating manual Accessories, Material and Hoses 4.5.3 Specifications Data Value Material in contact with 316L medium Surface in contact with medium Ra 0.8 µm, electropolished Material O-rings EPDM Page 120 of 284 08.01.2025...

  • Page 121: Inoculation Needles

    Techfors-S - Operating manual Accessories, Material and Hoses Inoculation Needles Kopiervorlagen diverse 4.6.1 Setup and Function Overview Kopiervorlagen diverse Kopiervorlagen diverse Kopiervorlagen diverse Kopiervorlage Bilderrahmen Kopiervorlagen diverse Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen Kopiervorlagen diverse Kopiervorlage Bilderrahmen hmen Kopiervorlage Bilderrahmen Protective cap (cannot be sterilized!), 2 x or 4 x...

  • Page 122: Preparing Inoculation Needles For Cultivation

    Techfors-S - Operating manual Accessories, Material and Hoses Function Inoculation needles are used to add liquids such as reagent, nutrient solution or the inoculation culture (inoculum) to the vessel. For this purpose, a septum, which is fixed with a septum collar in the port in the vessel top plate, is pierced with the inoculation needle.
  • Page 123 Techfors-S - Operating manual Accessories, Material and Hoses Autoclaving Inoculation Needles Proceed as follows: Carefully remove the protective caps from the inoculation needle. Get the septum collar ready for equipping the port in the vessel top plate. Connect the hose of the reagent bottle to the inoculation needle.

  • Page 124: Specifications

    Techfors-S - Operating manual Accessories, Material and Hoses 4.6.3 Specifications Data Value Material in contact with 316L medium Surface in contact with medium Ra 0.8 µm, electropolished Material O-ring EPDM Vessel Light 4.7.1 Setup and Function Torch Hollow screw Threaded housing with O-ring (a)

  • Page 125: Mounting The Vessel Light

    Techfors-S - Operating manual Accessories, Material and Hoses Switching the Vessel Light On and The torch can be switched on or off by turning it clockwise/counter- clockwise. 4.7.2 Mounting the Vessel Light The vessel light is mounted in a 19 mm port in the vessel top plate. To...
  • Page 126 Techfors-S - Operating manual Accessories, Material and Hoses Place the torch in the hollow screw. Page 126 of 284 08.01.2025...

  • Page 127: Transport And Storage

    Transport and Storage Inbound delivery and transport to the assembly location are per- formed only by INFORS HT employees or by persons authorized by INFORS HT. Nonetheless it is possible that the provider’s personnel is entrusted with transport tasks in the context of on-site transport. In this case, observe the following notes.

  • Page 128: Transportation Safety Device

    Techfors-S - Operating manual Transport and Storage Transportation Safety Device Kopiervorlagen diverse To protect the load cells from damage during transport, a transporta- tion safety device is fitted to the weight measurement frame. This means that two plates (1) are screwed to the right and left on the underside of the frame.

  • Page 129: Installation And Commissioning

    Techfors-S - Operating manual Installation and Commissioning Installation and Commissioning Only the manufacturer’s qualified expert personnel or persons author- ized by the manufacturer may install and initially commission the device. After installation of the device, the temperature control circuit must be filled first.

  • Page 130: Minimum Distances To The Device

    Techfors-S - Operating manual Installation and Commissioning Minimum Distances to the Device The device must be positioned at least 150 mm away from walls, ceilings and other devices. Furthermore, make sure that the vessel top plate with its built-in-parts can be easily lifted off and removed from the vessel. When calculating the distance between the device and the ceiling, this must be taken into account.
  • Page 131 Techfors-S - Operating manual Installation and Commissioning Exit Gas The exit gas must be dissipated securely by means of a suitable, ■ gas-tight hose. The exit gas line must be higher than the exit gas filter. ■ Water for Temperature Control Cir-...
  • Page 132 Techfors-S - Operating manual Installation and Commissioning Water Requirements for Integrated Water for the integrated steam generator must be of the fol- Steam Generator (if Available) lowing quality: CaCO concentration 0 mmol L up to a max. of 0.53497mmol L...

  • Page 133: Safety Valves

    Techfors-S - Operating manual Installation and Commissioning Safety Valves CAUTION If the safety valve of the vessel is triggered, steam, depending on the process phase and vessel contents of the bioreactor, hot and/or contaminated liquid or dangerous gases may escape from the over- pressure venting pipe.

  • Page 134: Cultivation

    Techfors-S - Operating manual Cultivation Cultivation This chapter describes the work necessary for preparing, performing and finalizing a cultivation. It also describes how the vessel is sterilized again, cleaned and prepared for a new cultivation. Preparing the Bioreactor CAUTION Uncontrolled rolling away of the device poses a risk of injury and damage to property.
  • Page 135 Techfors-S - Operating manual Cultivation type and length of the hose/piping on the overpressure venting pipe of the safety valve installed by the operator, however, this might have Kopiervorlagen diverse to be removed. Activity Additional Information Kopiervorlagen diverse Uncouple the motor.
  • Page 136 Techfors-S - Operating manual Cultivation Kopiervorlagen diverse Activity Additional Information Kopiervorlagen diverse If necessary, calibrate the pumps. This makes it possible to display ⮫ Chapter 8.8.2, page 221 the actual volume pumped in mL or g. The calibration of the pumps must be performed before autoclaving the reagent bottles.

  • Page 137: In Situ Sterilization

    Techfors-S - Operating manual Cultivation Kopiervorlagen diverse Activity Additional Information Kopiervorlagen diverse Prepare all additional (optional) sensors (e.g. level, pressure, tur- bidity, redox, pCO , permissiveness or O / CO in the exit gas). This is described in the chapter of the respective sensor in the main chapter “Options”.

  • Page 138: Preparing The Medium

    Techfors-S - Operating manual Cultivation Preparing the Medium Before the first sampling, which usually takes place as a “zero sample” before inoculation, and before the inoculation itself, the medium must be warmed to the desired temperature. If necessary, set the pO con- centration and the pH.

  • Page 139: Harvest

    Techfors-S - Operating manual Cultivation Methods There are different methods for adding inoculum. The exact method depends on the internal guidelines and on the system used and is defined by the operator. Harvest The culture can be harvested at the end of the cultivation. For example, get a suitable vessel ready or connect a hose to the harvest valve.
  • Page 140 Techfors-S - Operating manual Cultivation Use pumps to completely empty all hoses of the reagent bottles. Disconnect the hoses and remove them from the pumps. If in use: remove the inoculation needles from the ports in the vessel top plate under sterile conditions and close the ports with blanking plugs before in situ sterilization.

  • Page 141: Operation

    Techfors-S - Operating manual Operation Operation This chapter describes all touch screen software functions accessible to the operator in detail. It also describes the emergency shut-off and restarting after an emergency shut-off. NOTICE Changes to settings in the touch screen software by unqualified or untrained personnel can lead to malfunctions.

  • Page 142: Overview Screen, Menu Navigation And Control Elements

    Techfors-S - Operating manual Operation Overview Screen, Menu Navigation and Control Elements Sections Title bar Shows the device name, operating states, warning messages, login status and time. If an external software like eve accesses the OPC ® XML DA server of the touch screen software, this is indicated by two vertically opposed arrows in the title bar.
  • Page 143 Techfors-S - Operating manual Operation Main Menus The following main menus are available (from left to right): Main : shows parameters and their values, pumps or individual ■ valves of the bioreactor. Batch : this is where the cultivation and all sterilization processes ■...
  • Page 144 Techfors-S - Operating manual Operation Input Fields and View Boxes Input fields and view boxes are included in various menus, dialog boxes and tab pages. They require numeric or alphanumeric values to be entered or display these. Numeric Keypad and Alphanumeric...

  • Page 145: Main Menus

    Techfors-S - Operating manual Operation Main Menus 8.2.1 Main – Overview Main menu depicts the bioreactor and some of its valves graphi- cally and provides an overview of the process parameters and pumps, which vary depending on the device configuration.
  • Page 146 Techfors-S - Operating manual Operation Parameter Controlled process parameters and their actual values are displayed in the form of buttons. Pressing the button of a parameter leads to the Temperature parameter, parameter options. Example on the left: Setpoint option. Pumps The right part of the screen depicts all integrated peristaltic pumps of the bioreactor in the form of buttons.
  • Page 147 Techfors-S - Operating manual Operation Valves The red color indicates a closed valve. ■ The green color indicates an open valve. ■ A indicates that the valve is in automatic mode. The letter ■ M indicates that the valve is in manual mode, i.e. in a The letter ■...

  • Page 148: Batch - Start Menu

    Techfors-S - Operating manual Operation 8.2.2 Batch – Start Menu Batch main menu, the following activities can be performed. In the Starting and stopping the processes. ■ Calibrating the pH und pO sensors and pumps. ■ Filling/emptying the pump hoses.

  • Page 149: Controller - Value Display

    Techfors-S - Operating manual Operation 8.2.3 Controller – Value Display Controller main menu shows the actual values, setpoints and con- trol outputs of the parameters of the bioreactor. Parameter settings can be adjusted here. Menu Overview Designation Description Parameter Lists the parameters. Pressing a parameter button calls up its setting menu (⮫ Chapter 8.6, page 190).
  • Page 150 Techfors-S - Operating manual Operation Designation Description Cascade Shows whether and which cascade control is active and which process parameters Cascade main menu (⮫ chapter are used. Settings for cascade control are made in the 8.7, page 215). Output Displays the controller output of a parameter in %. A deactivated parameter is indi- cated with the word OFF.

  • Page 151: Cascades

    Techfors-S - Operating manual Operation 8.2.4 Cascades Cascades main menu provides the option of setting up a serial, parallel or mixed cascade control of a parameter. This function is mainly used for pO control. The cascade settings are made in the left-hand section of the screen and the main section presents these schematically.

  • Page 152: Trends - Trend Lines

    Techfors-S - Operating manual Operation 8.2.5 Trends – Trend Lines The touch screen operating panel keeps the actual values of the parameters in a buffer memory and displays them continuously as a Trends main menu. This data cannot be archived, edited diagram in the or exported.

  • Page 153: System - System Settings

    Techfors-S - Operating manual Operation 8.2.6 System – System Settings System main menu shows the following information on the Device Information device: Serial number ■ Software version ■ IP address(es) of the system ■ MAC address ) Device address ( ■...
  • Page 154 Techfors-S - Operating manual Operation Buttons The top right of the screen has two buttons: Statistics: makes it possible to view some statistics of the soft- ■ ware communication with the control board, or hardware. The only purpose of this function is to assist the manufacturer’s technical support with troubleshooting.

  • Page 155: Alarms - Alarm Display

    Techfors-S - Operating manual Operation 8.2.7 Alarms – Alarm Display 8.2.7.1 Menu Overview The Alarms main menu lists all process parameter alarms by time of occurrence. All system alarms are also displayed here. Alarm Display An alarm is indicated by the alternately light red-dark red flashing Alarms tab.

  • Page 156: Parameter Alarms

    Techfors-S - Operating manual Operation 8.2.7.2 Parameter Alarms A parameter alarm occurs as soon as the actual value of a parameter is outside the set alarm limits or critical values. : lower (14.3 < 15) Example Displayed alarm: ■ Meaning: the actual value of the parameter pO (= 14.3 %) has...
  • Page 157 Techfors-S - Operating manual Operation System Alarm Short Description Additional Information No water detected in temperature No water detected in temperature ⮫ Chapter 9.1.3, page 246 control system, refill failed control system. Difference in board System Alarm A backup of the control board configuration is stored in the touch configuration screen for the device.

  • Page 158: Submenus

    Techfors-S - Operating manual Operation Submenus 8.3.1 Valves – Digital Controller Outputs The submenu Valves displays the digital outputs and inputs of the control board. The overview is predominantly used for fault diagnosis. Auto ) ex- All valves and digital outputs are set to automatic mode ( All ) factory.

  • Page 159: Security - User Administration

    Techfors-S - Operating manual Operation 8.3.2 Security – User Administration 8.3.2.1 Menu Overview Security submenu is used for logging on and off to and from the system. Users can also be added or deleted here, passwords can be set and access rights can be assigned. The number of functions available depends on the authorisation of the user that is logged on: Login/Logout: log in to/out of the system.

  • Page 160: Access Rights

    Service Without access rights, is logged on automatically if no other user is logged on. Can only be accessed by qualified INFORS HT service technicians and is blocked for all other users. The passwords defined ex-factory should be changed and administrated by the authorized person Administrator ) during initial commissioning.
  • Page 161 Techfors-S - Operating manual Operation STERILIZATION User groups Guests Users Techn. Admin. Serv. Option: sterilization sample valve (SIP Sample Valve) Option: sterilization feed line (SIP Feed Line) Recipes ) RECIPES ( User groups Guests Users Techn. Admin. Serv. Load / Start...
  • Page 162 Techfors-S - Operating manual Operation PARAMETER options User groups Guests Users Techn. Admin. Serv. Slope and/or Offset (calibration pH analog: changing mode Manual) Calibrating the pO sensor (Calibrate pO analog: Use As Setpoint function in calibration menu Use As Setpoint function (if available) in all other cali-...
  • Page 163 Techfors-S - Operating manual Operation Alarms ) ALARMS ( User groups Guests Users Techn. Admin. Serv. Confirming an alarm (Confirm) System ) SYSTEM ( User groups Guests Users Techn. Admin. Serv. Viewing the statistics of the communication software with the bioreactor hardware (Statistics)
  • Page 164 Techfors-S - Operating manual Operation System / Settings ) SYSTEM SETTINGS ( User groups Guests Users Techn. Admin. Serv. Network settings (IP Settings) Changing the date and time (Change Time) Saving data (Backup) Restoring data backups (Restore) Service menu Exporting log files (Export Logs)

  • Page 165: (Own) Password

    User ■ Technician ■ Administrator ■ Other : only for INFORS HT service staff ■ Default : automatic user login without password input, if ■ previously configured via Set Default User. Technician . Select the desired user, e.g.

  • Page 166: New User - Adding A New User

    Techfors-S - Operating manual Operation Enter the old password. Enter the new password and confirm by entering it again. �� All inputs are displayed as dots. Depending on the password rules set, the password must meet various condi- tions. Password rules can be configured by Administrator users.

  • Page 167: Edit User - Changing User Settings

    Techfors-S - Operating manual Operation Logout if Activate or deactivate the automatic user logout ( inactive ) after expiry of a defined time period of screen inac- Logout after, min ) if necessary. tivity, and set a time limit ( Press OK.

  • Page 168: Remove User - Deleting A User

    Techfors-S - Operating manual Operation 8.3.2.8 Remove User – Deleting a User To delete a user, proceed as follows: Administrator call up the Security submenu. TEST ) from the selection list and Select the desired user (here: press Remove User.

  • Page 169: Password Security - Setting Password Rules

    Techfors-S - Operating manual Operation TEST ) from the selection list and Select the desired user (here: press Set Default User. �� The defined user for automatic user login is now displayed with a bold font, the Set Default User button is still visible but no longer available.
  • Page 170 Techfors-S - Operating manual Operation Password rules : a drop-down list with four password rules ■ is available (see figure above). The password must contain at least: – 8 characters, which have to include at least 1 number, 1 upper case letter and 1 lower case letter –...

  • Page 171: Settings - Basic Settings Of The Device

    ■ Files Backup: save data. ■ Restore: upload saved data to the system. ■ Service Menu: accessible only to INFORS HT service technicians or ■ authorized licensed dealers. Export Logs: export log files. ■ Controller Board Configuration (Con- Input Channel Code: set codes for input channels.

  • Page 172: Ip-Settings - Network Settings

    Techfors-S - Operating manual Operation Extended Digital Output Function Code: set functions codes for ■ extended digital outputs. Synchronize differing board configuration: synchronize differing ■ board configuration. This button only appears if a corresponding alarm Difference in board configuration ) has been triggered...

  • Page 173: Change Time

    Techfors-S - Operating manual Operation Obtain IP settings automatically: automatically apply IP settings ■ (default settings). Prerequisite: a DHCP server is available in the network. Use the following IP settings: use the following IP settings. An ■ entry can only be made in the following fields after pressing this button.

  • Page 174: Backup - Saving Data

    Techfors-S - Operating manual Operation Press Change Time. �� The Change System time dialog box appears with the config- uration set ex-factory: Set time and date automatically : activate or deactivate ■ automatic time and date setting. This function is activated by default.

  • Page 175: Restore - Restoring Data Backups

    Techfors-S - Operating manual Operation Files section, press Backup. In the �� The Confirmation dialog box appears with a note and a prompt for switching to backup mode. Press OK. �� The data backup menu appears. Select backup destination : choose a storage location, ■...
  • Page 176 Techfors-S - Operating manual Operation Please note: The data is loaded either from the internal storage or from a USB ■ stick, see ⮫ Chapter 8.3.3.4 ‘ Backup – Saving Data’ on page 174. Restore function can only be executed when all processes are ■...

  • Page 177: Export Logs - Exporting Log Files

    (not shown here). The view of the differences within a file is primarily intended as information for the INFORS HT service technician. It represents the differences of the settings file to be restored compared to the version currently used in unified format (also unidiff).

  • Page 178: Balance Settings

    Techfors-S - Operating manual Operation Please note: A USB stick is required for the data export. ■ A data export can only be executed when all processes have ■ stopped. To export the log files, proceed as follows: Connect the special cable supplied by the device manufacturer to the connection socket on the back of the operating panel and connect the USB stick.
  • Page 179 Techfors-S - Operating manual Operation Balances connected : Select the number of balances in None : none ■ Single : one balance; this means that the first drop-down list ■ in the menu area becomes available. Infors Switchbox : switchbox, this makes all 7 drop-down lists ■...

  • Page 180: Wipe Screen - (Temporarily) Locking The Screen

    Techfors-S - Operating manual Operation 8.3.4 Wipe Screen – (Temporarily) Locking the Screen Wipe Screen submenu has only one function: it locks all input on the screen for 20 seconds. This enables you to clean the screen within 20 seconds, if required.

  • Page 181: Shutdown - Shutting Down The System

    Techfors-S - Operating manual Operation 8.3.5 Shutdown – Shutting Down the System Shutdown submenu has only one function: it shuts down the system. This is only possible when all processes have stopped. ALWAYS shut down the system first, then switch off...

  • Page 182: Recipes

    Techfors-S - Operating manual Operation Recipes 8.4.1 General Information Recipes function in the Batch main menu The different buttons of the can be used to load and start, store or delete what are referred to as recipes. That is, the parameter settings (including cascades) of a cultivation can be stored and reused when the same work processes occur again.

  • Page 183: Delete Recipe

    Techfors-S - Operating manual Operation Here, all parameters used in the recipe are listed. Setpoints can be changed subsequently and parameters can be acti- vated or deactivated. Use OK to start the cultivation. If necessary, change the setpoint and/or activate/deactivate parameters.
  • Page 184 Techfors-S - Operating manual Operation Press OK. �� The Confirmation dialog box appears to confirm the deletion process. Press OK. �� The recipe has been deleted. Page 184 of 284 08.01.2025...

  • Page 185: Parameters

    Techfors-S - Operating manual Operation Parameters This chapter contains brief descriptions of all parameters. In addition to all parameters that are available by default in every device configu- ration, this also includes those parameters that are only configured if the corresponding device option is available.
  • Page 186 Techfors-S - Operating manual Operation Antifoam Detects foam and controls the addition of antifoam agent. The anti- foam pump is activated as soon as the antifoam sensor comes in con- Output ) shows 100 % instead tact with foam. The parameter output ( of 0 %.
  • Page 187 Techfors-S - Operating manual Operation GasMix Controls the oxygen concentration in the inlet air. This is achieved by switching between air and oxygen or air and nitrogen for a 2-gas-mix system or air, oxygen and nitrogen for a 3-gas-mix system. Depending...
  • Page 188 Techfors-S - Operating manual Operation Example of a 2-gas-mix system with The solenoid valves are switched according to the pre-set cycle time PID parameter option of the GasMix parameter. Air und O with solenoid valves in the Settings: Eval. Time (s) in option Cycle time: 10 seconds (visible in input field ■...
  • Page 189 The sensor with the corresponding transmitters must be purchased directly from the manufacturer ABER. INFORS HT offers a connection to the transmitter on the basic unit. Capacitance Measures the capacity (measured in pF cm ) that correlates to the live biomass.

  • Page 190: Parameter Options

    PID : this is where the controller settings are made. ■ Options : only qualified INFORS HT personnel has access to this. This ■ option is not available nor visible to any other user group. If the weight measurement for the vessel is available, the weight display can be tared via Tare Weight in Batch main menu.

  • Page 191: Setpoint

    Techfors-S - Operating manual Operation 8.6.2 Setpoint 8.6.2.1 Menu Content Setpoint : set the setpoint. ■ Value : shows the current measurement (actual value). ■ Output : shows the controller output in % or OFF . ■ Lower Critical and Upper Critical : set the lower and the upper ■...

  • Page 192: Setting Setpoints, Activating And Deactivating Parameters

    Techfors-S - Operating manual Operation 8.6.2.2 Setting Setpoints, Activating and Deactivating Parameters General Information The setpoints of parameters are generally set in the configuration dialog of the cultivation. While the process is running, these can be Controller main menu. changed via the The same applies for activating and deactivating parameters.
  • Page 193 Techfors-S - Operating manual Operation Settings while the Cultivation is Run- There are two options for entering settings while the cultivation is ning running: Setpoint input field/view box and con- Variant A: directly via the ■ Output column troller output button in the...

  • Page 194: Setting Alarm Limits And Critical Values

    Techfors-S - Operating manual Operation For variant B), proceed as follows: In the Controller main menu, press the button of the desired parameter. Setpoint tab page appears. �� The Set the setpoint and, if necessary, change alarm limits and crit- ical values.

  • Page 195: Calibrate

    Techfors-S - Operating manual Operation 8.6.3 Calibrate 8.6.3.1 Menu Content Reading : shows the current measurement in digital units. ■ Value : shows the current measurement, depending on the last ■ calibration. Slope : shows the digital value of the calculated linear slope.

  • Page 196: Calibrating A Digital Ph Sensor

    Techfors-S - Operating manual Operation 8.6.3.3 Calibrating a Digital pH Sensor General Information The pH buffers and their temperature dependence are saved in the digital pH sensors and are automatically detected during calibration. It is therefore not necessary to carry out a separate temperature measurement of the buffer solution.
  • Page 197 Techfors-S - Operating manual Operation Batch main menu, press Calibrate pH. In the �� The calibration menu opens with four options: 1-Point Calibration and 2-Point Calibration: select 1- ■ point or 2-point calibration. Product Calibration: select product calibration. ■ Show Sensor Status: menu with dates and values that ■...
  • Page 198 Techfors-S - Operating manual Operation Hold the pH sensor in the appropriate buffer solution of the first calibration point and, if possible, select reference value or automatic buffer recognition in drop-down list (step 1). HAMILTON Easyferm Plus ARC: Always start with the lower calibration buffer (4.01).

  • Page 199: Product Calibration Of A Digital Ph Sensor

    Techfors-S - Operating manual Operation Sensor Status SHOW SENSOR STATUS is used to show data and values produced by the firmware of the sensor manufacturer integrated in the sensor. In addition to information on the sensor type and information on...
  • Page 200 Techfors-S - Operating manual Operation Step 1 and 2: confirm product calibration via START and ■ Sample sampling via CONFIRM to generate a time stamp ( was taken at ). Status display of the calibration with the following pos- sible displays: ready : time stamp for completed sampling can be gen- –...

  • Page 201: Calibrating An Analog Ph Sensor

    Techfors-S - Operating manual Operation Press CONFIRM. �� The status display changes to measured . The date and time of sampling are now displayed. Carry out a laboratory measurement of the pH value for the sample. Enter the measured pH value of the sample, in the example to the left that is pH 7.0.
  • Page 202 Techfors-S - Operating manual Operation Calibrating Proceed as follows: Connect the sensor cable. Ensure the sensor cable is not buckled or twisted. NOTICE The screening on the sensor cable may become damaged if it is kinked or twisted. This may lead to faulty measurements.
  • Page 203 Techfors-S - Operating manual Operation Enter the value of the lower (or upper) reference button in the input field in line 1. The order in which the reference points are cali- brated is irrelevant. If temperature compensation is active: Ref. Temp...

  • Page 204: Recalibrating An Analog Ph Sensor

    Techfors-S - Operating manual Operation 8.6.3.6 Recalibrating an Analog pH Sensor General Information To compensate for a deviation (drift) of a measurement of an analog pH sensor during a long-term cultivation, a re-calibration is possible and sufficient. There are two different ways in which this can be done: 1-point calibration ■...

  • Page 205: Calibrating Digital A Po Sensor

    Techfors-S - Operating manual Operation Depending on the difference between the two pH measure- ments, either add the result to the displayed "Offset" or subtract it and enter it. Press OK. �� The calibration menu disappears, the value is saved.
  • Page 206 Techfors-S - Operating manual Operation Batch main menu, press Calibrate pO In the �� The calibration menu opens with three options: 1-Point Calibration and 2-Point Calibration: select 1- ■ point or 2-point calibration. Show Sensor Status: shows dates and values that are ■...
  • Page 207 Techfors-S - Operating manual Operation 100 (= 100 %) in the drop- If possible, select reference value down list (step 1). Wait until the measurement is stable (step 2). Press CALIBRATE (step 3a). �� The calibration process starts. The CALIBRATE button turns into CONFIRM.

  • Page 208: Calibrating An Analog Po Sensor

    Techfors-S - Operating manual Operation 8.6.3.8 Calibrating an Analog pO Sensor General Information Polarographic pO sensors must be polarized for com- missioning or after they have been disconnected from the power source. Otherwise, correct calibration is not possible. For detailed information on polarization, refer to the separate documentation provided by the sensor manufacturer.

  • Page 209: Calibrating The Turbidity Sensor

    ■ trol. For all other parameters, the Use As Setpoint button is only relevant for INFORS HT service technicians. How it works: 0 % calibration: the input 0 (%) in the input field of the first calibra- ■ tion point and pressing the USE AS SETPOINT button causes the GasMix parameter to switch to nitrogen for this value.

  • Page 210: Pid Controller Settings

    Techfors-S - Operating manual Operation Calibrating Proceed as follows to calibrate the zero point of the (optional, only for the Optek system) turbidity sensor: Connect the sensor cable. Controller main menu and wait until the measure- Call up the ment of the Turbidity parameter is stable.

  • Page 211: Pid Control Explained

    Techfors-S - Operating manual Operation Setting Description D (Diff Term [s]) The differential quotient calculates the change in the actual value over time and counteracts this change. Neg. Factor The negative factor can be used to weight a two-sided control (+100 to -100 percent) (e.g.
  • Page 212 Techfors-S - Operating manual Operation Error = deviation between setpoint and actual value The Formula Explained ■ P = proportional factor, also called slope, with which a setpoint is ■ reached. The larger the value, the faster the control. I = Integral factor of the error in 1/second. A typical integral factor is ■...

  • Page 213: Tips For Readjusting A Pid Controller

    Techfors-S - Operating manual Operation Example for Calculating the Integral The period of oscillation of the system is detected with 50 seconds Factor from amplitude to amplitude. The integral factor is calculated as fol- lows then: 1 / (50 s x 2) = 0.01 s 1 / (50 s x 4) = 0.005 s...

  • Page 214: Tare Weight Function - Taring The Weight Display

    Techfors-S - Operating manual Operation 8.6.5 Tare Weight Function - Taring the Weight Display Batch main menu is generally used to define the Tare Weight in the zero point of the vessel weighing system. This sets the vessel weight to zero (tares it) so that only the weight of the vessel contents can be measured.

  • Page 215: Cascade Control

    Techfors-S - Operating manual Operation Cascade Control 8.7.1 Cascade Control Explained Cascade main menu provides the option of setting up a cascade control of a process parameter, usually pO . This means that the con- Output ) of the primary parameter (e.g. pO...

  • Page 216: Setting A Cascade

    Techfors-S - Operating manual Operation 8.7.2 Setting a Cascade 8.7.2.1 Menu and Functions Overview The various cascade settings are made in the left menu area. To acti- vate the view of the existing process parameters in the main area and...
  • Page 217 Techfors-S - Operating manual Operation Input / Function Description Advanced Activate or deactivate the setting mode for an advanced cascade. Advanced cascades are used for customer-specific device configu- rations. They are set exclusively by the device manufacturer ex-fac- tory. In this case, their settings and change options are created for the specific device and can be requested from the manufacturer.

  • Page 218: Cascade Progress Display

    Techfors-S - Operating manual Operation 8.7.2.2 Cascade Progress Display Controller main menu. Overview A cascade and its progress can be seen in the In addition to arrows showing the direction of the cascade control, the setpoint and the control output of the cascade that is added to Cascade column.

  • Page 219: Deleting A Cascade

    Techfors-S - Operating manual Operation Color Value range utilisation Grey Inactive Green 0 to 90 % Yellow 90 to 99 % 100 % Blue Stirrer parameter is used as the example for secondary parameter Calculation Example from the setpoint to the max. setpoint.

  • Page 220: Special Configurations

    Techfors-S - Operating manual Operation 8.7.2.4 Special Configurations For bioreactors with the "High-End" gassing strategy (configuration with several mass flow controllers and GasMix), the gases to be used, Air Flow , N Flow and O Flow must be assigned to the two e.g.

  • Page 221: Pumps

    Techfors-S - Operating manual Operation Pumps 8.8.1 Control and Settings Control The peristaltic pumps are controlled in accordance with the respective parameters: Pump Parameter Acid (digital) Base (digital) Antifoam (digital) Antifoam Feed (analog) Feed Feed 2 / Feed 3 (analog, optional)
  • Page 222 Techfors-S - Operating manual Operation Tools Provide the following tools: Graduated measuring cylinder/cup or balance and empty vessel. ■ Reagent bottle equipped with silicone hose and filled with the ■ reagent to be pumped or equally viscous liquid. For very precise results, the reagent bottle should be placed on a balance that can also be connected to the bioreactor or a PC with the eve®...

  • Page 223: Resetting The Pump Counter To Zero

    Techfors-S - Operating manual Operation Press OK. �� Calibration starts. The remaining time ( time left:…. ) in h/min/s is displayed next to the Stop button that is now available. As soon as this time has expired, the second dialog box appears ( Calibrate Acid Pump Part 2 ).

  • Page 224: Filling And Emptying The Pump Hoses

    Techfors-S - Operating manual Operation 8.8.4 Filling and Emptying the Pump Hoses The pump hoses can be filled and emptied either in a time-controlled manner or manually. Time controlled filling or emptying of the pump hoses is only possible only when the cultivation is stopped.
  • Page 225 Techfors-S - Operating manual Operation Here, an individual filling duration and empty duration in seconds can be defined for each pump. The filling and emptying process is started via Fill and Empty. Next to each of these buttons is a button for immediately stopping the current fill / empty procedure.

  • Page 226: Processes

    CIP-enabled. However, the CIP process is controlled via the separate TechCIP mobile CIP unit of the INFORS HT device manufac- turer. The instructions for this are available in the sep- arate operating manual of the mobile CIP unit.

  • Page 227: Safety Notes

    Techfors-S - Operating manual Operation Process Abortion There are two different types of process abortions: Automatic process abortion triggered by the system due to a ■ faulty system state. Manual process abortion, triggered by the operator via Stop. ■ In any case, a safe abortion defined via the system takes place. A aborted at with date and time.

  • Page 228: Full Sterilization

    Techfors-S - Operating manual Operation 8.9.3 Full Sterilization 8.9.3.1 Process Sequence For full sterilization, the water in the vessel jacket is heated by feeding in steam. The steam generated by the liquid in the vessel also sterilizes the inlet air and exit gas filters at the same time.

  • Page 229: Process Configuration

    Techfors-S - Operating manual Operation Process step D = Dialog box, user interaction required S = Status display, no user interaction Holds holding phase temperature until cultivation is started or full sterilization is stopped. completed at with date and time in h:min:s...

  • Page 230: Before Starting The Process

    Techfors-S - Operating manual Operation Input field Value range Unit Start inhibitor (activate or deactivate No / Yes start delay) Hours 0 to 99 Minutes 0 to 59 (Start process in ... hours and ... minutes) 8.9.3.3 Before Starting the Process Before starting the process, check and ensure that: All required services are available and activated.

  • Page 231: Terminating The Process

    Techfors-S - Operating manual Operation The figure shows, as an example, the dialog box of a device with a rotameter, gas sensors for exit gas analysis, ball valves for switching from tap water to chilled water and a resteriliz- able feed line.

  • Page 232: Aborting The Process

    Techfors-S - Operating manual Operation 8.9.3.6 Aborting the Process For safety reasons, an immediate process abortion is only possible at a temperature of < 70 °C. That is, at ≥ 70 °C the cool down phase is initiated first. 8.9.4 SIP Harvest/Sample Valve –...

  • Page 233: Before Starting The Process

    Techfors-S - Operating manual Operation 8.9.4.3 Before Starting the Process Before starting the process, check and ensure that: All required services are available and activated. ■ All services have the correct connection pressure. ■ 05.12.01 is closed. The harvest/sample valve ■...

  • Page 234: Aborting The Process

    Techfors-S - Operating manual Operation Press OK. �� The process is finalized. 8.9.4.5 Aborting the Process The process can be aborted at any time. The same dialog box appears as for the normal process end. 8.9.5 SIP Sample Valve – Sterilization Sample Valve 8.9.5.1...

  • Page 235: Before Starting The Process

    Techfors-S - Operating manual Operation 8.9.5.3 Before Starting the Process Before starting the process, check and ensure that: All required services are available and activated. ■ All services have the correct connection pressure. ■ 17.13.01 is closed. Sample valve ■...

  • Page 236: Aborting The Process

    Techfors-S - Operating manual Operation Press OK. �� The process is finalized. 8.9.5.5 Aborting the Process The process can be aborted at any time. The same dialog box appears as for the normal process end. 8.9.6 SIP Feed Line – Sterilization Feed Line 8.9.6.1...

  • Page 237: Before Starting The Process

    Techfors-S - Operating manual Operation 8.9.6.3 Before Starting the Process Before starting the process, check and ensure that: All required services are available and activated. ■ All services have the correct connection pressure. ■ 13.16.02 / 13.16.04 is connected. Block valve ■...

  • Page 238: Aborting The Process

    Techfors-S - Operating manual Operation Execute the steps of the procedure in the order listed. Press OK. �� Ventilation starts. As soon as the set ventilation time has elapsed, the third dialog box appears with instructions. Execute the step. Press OK.

  • Page 239: Bioreactor Operation - Cultivation

    Techfors-S - Operating manual Operation 8.9.7 Bioreactor Operation – Cultivation 8.9.7.1 Process Sequence Process step D = Dialog box, user interaction required S = Status display, no user interaction configuration for process configuration Configuration user interaction required with instructions User interaction...

  • Page 240: Before Starting The Process

    Techfors-S - Operating manual Operation The setting range depends on the type and number of gases used (air, O Depending on the selected gassing strategy and the number of gases, more or fewer Flow parameters exist and are configured. 8.9.7.3...

  • Page 241: Terminating The Process

    Techfors-S - Operating manual Operation The figure shows, as an example, the dialog box of a device with a rotameter, gas sensors for exit gas analysis, ball valves for switching between tap water/chilled water and resteriliz- able feed line. Execute all the steps of the procedure in sequence.

  • Page 242: Shutting Down The System; Switching Off The Device

    Techfors-S - Operating manual Operation 8.10 Shutting Down the System; Switching off the Device To shut down the system and switch off the device, proceed as fol- lows: System main menu, press Shutdown. In the Confirmation dialog box for confirming the shutdown ��...

  • Page 243: Gency

    Techfors-S - Operating manual Operation 8.11 Emergency Shut-Off – Shut Down in Case of an Emergency To shut down the device in dangerous situations, proceed as follows: Immediately switch off the device at the main switch. �� The device is de-energized.

  • Page 244: Rectifying Faults

    Techfors-S - Operating manual Rectifying Faults Rectifying Faults The following chapter describes possible reasons for faults and how to resolve them. Reduce the service intervals in correspondence with the actual loads if faults become increasingly common. Contact the manu- facturer or licensed dealer in case of faults that cannot be resolved by following the notices below.

  • Page 245: Faults Drive System

    Techfors-S - Operating manual Rectifying Faults 9.1.2 Faults Drive System Fault description Cause Remedy Personnel Stirrer parameter is not Stirrer parameter. Motor does not start. Activate the Operator activated. Setpoint of Stirrer param- Set setpoint > 0. Operator eter = 0.

  • Page 246: Faults Temperature Control System

    Techfors-S - Operating manual Rectifying Faults 9.1.3 Faults Temperature Control System Fault description Cause Remedy Personnel Temperature No temperature control. Temperature control has not Activate the Operator been activated. parameter. The stirrer has not been acti- Activate the Stirrer param-...

  • Page 247: Faults Gassing System

    Techfors-S - Operating manual Rectifying Faults 9.1.4 Faults Gassing System Fault description Cause Remedy Personnel No gassing. The on-site gas supply has Stop the cultivation. Operator been interrupted. Check the on-site gas supply and switch it on, if necessary. If available: the rotameter is...

  • Page 248: Faults Ph Control

    Techfors-S - Operating manual Rectifying Faults 9.1.5 Faults pH Control Fault description Cause Remedy Personnel No display or incorrect Sensor cable is not con- Connect the sensor cable Operator display of pH. Digital nected or not properly con- correctly. measurement systems: nected.
  • Page 249 Techfors-S - Operating manual Rectifying Faults Fault description Cause Remedy Personnel No pH control. No addition of reagents Open the push valve(s) (acids and base). if necessary. The (base/acid) pump does Check the function of Operator not operate properly. the pump using the rocker switch.

  • Page 250: Faults Po

    Techfors-S - Operating manual Rectifying Faults 9.1.6 Faults pO Control Fault description Cause Remedy Personnel No display or incorrect Sensor cable is not con- Connect the sensor cable cor- Operator display of pO Digital nected or not properly con- rectly.

  • Page 251: Faults Antifoam Control

    Techfors-S - Operating manual Rectifying Faults 9.1.7 Faults Antifoam Control Fault description Cause Remedy Personnel Foam is not detected, The sensor cables are not con- Connect the sensor cable Operator frequently detected or nected correctly. correctly. constantly detected. The insulation of the antifoam...

  • Page 252: Faults Feed And Pump

    Techfors-S - Operating manual Rectifying Faults 9.1.8 Faults Feed and Pump Fault description Cause Remedy Personnel Feed parameter is not Feed param- No or inadequate flow Activate the Operator of liquid via the feed active. eter. pump. Setpoint of the Feed parameter Set the setpoint >...

  • Page 253: Interferences Turbidity Measurement

    Techfors-S - Operating manual Rectifying Faults 9.1.9 Interferences Turbidity Measurement Fault description Cause Remedy Personnel Displayed measured Sensor cable is twisted or Check the connection Operator value is not plausible/ kinked or not properly con- of the sensor cable, unusual.

  • Page 254: Returning For Repair

    Techfors-S - Operating manual Rectifying Faults Returning for Repair The provider must return the device or the faulty component part(s) to the manufacturer if, after consulting the service department of the local dealer or the manufacturer, on-site diagnosis and/or repair is not possible.

  • Page 255: 10 Cleaning And Maintenance

    Techfors-S - Operating manual Cleaning and Maintenance 10 Cleaning and Maintenance The following chapters contain general descriptions regarding the cleaning of the vessel and its accessories, and how these can be stored. In addition, the chapter contains a maintenance plan and corre- sponding descriptions for the procedures to be performed by the operator.

  • Page 256: Cleaning The Bioreactor

    Techfors-S - Operating manual Cleaning and Maintenance 10.2 Cleaning the Bioreactor Overview of the Activities The following checklist lists all the activities that must be carried out Kopiervorlagen diverse as part of routine cleaning (e.g. after cultivation). Activity Additional Information Kopiervorlagen diverse Clean the vessel.

  • Page 257: Maintenance Plan

    Techfors-S - Operating manual Cleaning and Maintenance Cleaning the Sensors Cleaning and maintenance of the individual sensors are described in the corresponding, separate documentation of the respective sensor manufacturer. Read these manuals and follow the instructions therein. Sensors from the device manufacturer (antifoam and level) are cleaned and maintained like other built-in-parts, e.g.
  • Page 258 Techfors-S - Operating manual Cleaning and Maintenance Interval Maintenance work Personnel Prior to each use Check the reagent bottles and all other glass items for Operator damage, and replace if necessary. Lubricate the mechanical seal (for details, see Operator ⮫ Chapter 2.7.3 ‘Lubricating the Mechanical Seal’...
  • Page 259 Techfors-S - Operating manual Cleaning and Maintenance For detailed information on care and maintenance of built-in-parts and accessories from other manu- facturers, please refer to the documentation of the respective manufacturers and follow the instructions contained therein. This applies in particular to the safety valves.

  • Page 260: 11 Disassembly And Disposal

    Techfors-S - Operating manual Disassembly and Disposal 11 Disassembly and Disposal The device must be disassembled and disposed of in an environmen- tally-friendly manner if it is not in use anymore. If the device is to be returned to the manufacturer for...
  • Page 261 Techfors-S - Operating manual Disassembly and Disposal For disposal, the system units must be disassembled and dismantled into individual material groups. These materials must be disposed of according to the applicable national and local legislation. Local author- ities or specialist disposal firms can provide information regarding environmentally acceptable disposal.

  • Page 262: 12 Technical Data

    Techfors-S - Operating manual Technical Data 12 Technical Data 12.1 Dimensions Dimensions with options Weight measurement A = 1354 / B = 1870 Steam generator C = 876 Switch between tap water/chilled water D = 872 Switch between tap water/chilled water...

  • Page 263: Connections And Connection Values

    Techfors-S - Operating manual Technical Data 12.2 Connections and Connection Values Overview of Connections Exit gas Condensate (contaminated) out Water in steam generator (option) 10 Water out steam generator (option) Air in Tap water in (option) Tap water out 12 Chilled water in (option)
  • Page 264 Techfors-S - Operating manual Technical Data Connection Values Pos. Connection Connection type Connection value Exit gas Hose nozzle DN13 (no backpressure) Hose nozzle DN8 3.0 to 6.0 bar Hose nozzle DN8 3.0 to 6.0 bar Air in Hose nozzle DN8 3.0 to 6.0 bar...

  • Page 265: Specifications Of The Basic Unit

    Techfors-S - Operating manual Technical Data 12.3 Specifications of the Basic Unit 12.3.1 Instrumentation Cabinet Data Value Unit Width 320 mm Depth 450 mm Height 550 mm Protection type IP43   Material 1.4301   12.3.2 Operating Panel Data Value Color touch screen 12''...
  • Page 266 Techfors-S - Operating manual Technical Data Dimensions Vessel TV Height Ratio H/D 15 L 508 mm 200 mm 2.5 : 1 30 L 646 mm 250 mm 2.5 : 1 42 L 761 mm 267 mm 2.9 : 1 Without top plate and harvest/sample valve...

  • Page 267: Temperature Control System

    Techfors-S - Operating manual Technical Data Ports and Ingold Nozzles Vessel top plate port 15 L TV 30 L TV 42 L TV Tri-Clamp ISO DN25/1, ID = 50.5 mm (exit gas) Tri-Clamp ISO DN08, ID = 50.5 mm (inlet air/gas) ID = 19 mm (Rd28x1/8")

  • Page 268: Stirrer

    Techfors-S - Operating manual Technical Data Accuracy Measurement and Control Data Value Unit (Cultivation) Accuracy at ≤ 60 °C ± 0.3 °C Accuracy at > 60 °C ± 0.5 °C 12.3.5 Stirrer Drive Data Value Type Top, with single mechanical seal...

  • Page 269: Gassing System

    Techfors-S - Operating manual Technical Data Impellers Data Value Type Rushton impeller with 6 blades Material AISI 316L Surface Ra 0.8 µm, electropolished Dimensions and quantity of impellers Vessel TV Quantity 15 L 66 mm 13 mm 16 mm 30 L...
  • Page 270 Techfors-S - Operating manual Technical Data Control Ranges of MFCs and Rota- Vessel size Basic (rotameter) Standard/High meter End (MFC) 15 L TV (max. WV 10 L) 2.0 to 20.0 L min 0.20 to 20.0 L min 30 L TV (max. WV 20 L) 4.0 to 40.0 L min...

  • Page 271: Gassing Variants

    Techfors-S - Operating manual Technical Data 12.3.6.2 Gassing Variants General Data Value Gas entry Ring sparger Gas(es) Air / Air + O / Air + N Air + O Basic Variant Data Value Gas flow control 1 rotameter Gasmix control (if present)

  • Page 272: Exit Gas System

    Techfors-S - Operating manual Technical Data 12.3.7 Exit Gas System Exit Gas Cooler Data Value Material Stainless steel Filter Data Value Type Novasip, can be sterilized with steam Model C3PFRP1A Data Value Unit Max. pressure 6.5 bar Max. temperature 142 °C Retention rate 0.2 µm...
  • Page 273 Measurement range pH 0 to pH 14 The pH sensors of the Easyferm Plus ARC type have been preconfigured by the INFORS HT device man- ufacturer. Replacement sensors must be configured before use. Measurement System Digital Data...

  • Page 274: Control

    Techfors-S - Operating manual Technical Data 12.3.9 Control Data Value Control via cascade Parameters in cascade are dependent on device configura- tion Data Value Unit Control range 0 to 100 %-sat Measurement accuracy ± 1 % Analog Measurement System Data...

  • Page 275: Antifoam Control

    Value Unit Allowed temperature 0 to 60 °C Measurement range 0 to 285 %-sat Digital pO sensors have been preconfigured by the INFORS HT device manufacturer. Replacement sensors must be configured before use. 12.3.10 Antifoam Control Data Value Sensor Conductive with dosing needle,...

  • Page 276: Vessel Pressure Display (Manometer)

    Techfors-S - Operating manual Technical Data Analog Pumps Data Value Type Peristaltic Feed ) Default number Feed 2, Feed 3 ) Number of optional ones Data Value Unit Speed 0 to 150 min Setting range (0.1 % increments) 0 to 100 Accuracy ±...

  • Page 277: Safety Valves

    Techfors-S - Operating manual Technical Data 12.3.13 Safety Valves Vessel Safety Valve Data Value Type Clean service spring-loaded safety valve Data Value Unit Response pressure barg Temperature Control Circuit Safety Data Value Valve Type Standard safety valve, angle type, spring-loaded...

  • Page 278: Recirculating Chiller

    Techfors-S - Operating manual Technical Data Electric connection values Type Voltage Frequency Max. rated Connector range current 6 kW 400 V (± 5 %); 3 phases L1 + L2 + L3 + N 50 / 60 Hz 14 A CEE16/5...

  • Page 279: Pressure Control

    Techfors-S - Operating manual Technical Data 12.4.4 Pressure Control Data Value Sensor type Piezo-resistive pressure transmitter Control Proportional valve with electronic open-loop control Data Value Unit Measurement range -1 to +3 Measurement range 0 to 2.0 Control range 0 to 1.5 Measurement and control ±...

  • Page 280: Turbidity Measurement

    Techfors-S - Operating manual Technical Data 12.4.6 Turbidity Measurement Data Value Sensor type ASD25-N Optical path length OPL01, OPL05 or OPL10 Measurement principle Single channel light absorption Data Value Unit Absorption measurement range 0 to 4 CU Measurement wavelength 840 to 910 nm...

  • Page 281: Pco

    Techfors-S - Operating manual Technical Data 12.4.8 Measurement Data Value Sensor type InPro5000i, ISM (digital) Measurement principle Potentiometric Transmitter type M400 Data Value Unit Measurement range 0 to 1000 12.4.9 Redox Measurement METTLER Analog Measurement Data Value System Sensor type...

  • Page 282: Permissive Measurement

    Techfors-S - Operating manual Technical Data 12.4.10 Permissive Measurement Data Value Measurement system (sensor ABER Futura and transmitter) Data Value Unit Permittivity 0 to 400 pF cm Conductivity 0 to 40 mS cm All information about the ABER Futura system is available in the sepa- rate documentation provided by the manufacturer.
  • Page 283 Techfors-S - Operating manual Technical Data 12.7 Emissions Data Value Unit Sound pressure level < 70 dB(A) 08.01.2025 Page 283 of 284...
  • Page 284 Digitize your bioprocesses The platform software for your bioprocesses – the Bioprocess Platform Software ® Able to do more than just plan, control and analyze your bioprocesses, eve ® software integrates workflows, devices, bioprocess information and big data in a platform that lets you organize your projects in the cloud, no matter how complex they are.

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