Figure 2-4. Filter Holder Components - Sutter Instrument LAMBDA 10-B Operation Manual

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Retaining Ring. This ring is threaded on its outside surface and has two slots on its top
edge. The purpose of the two slots is for the removal and installation of the retaining ring
when using the retaining ring driver or spanner wrench that is provided with the
LAMBDA 10-B (shown in Figure 2-4). Note how the design of the spanner wrench differs
between 25-, 32-, and 50-mm filter sizes.
1. Spacer Ring. This ring is unthreaded and serves to evenly carry the force of the retaining
ring to the bottom of the filter cup or holder. It is recommended that the spacer be used
anytime a blanking disc, or the filter cup or holder is installed. The spacer ring should
also be used whenever the thickness of an installed filter allows its use. Maximum filter
thicknesses for the filter cup and holders are specified in Table 2-1.
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Blanking Disc. In all but one of the filter cups (and one of the filter holders) there will
also be a blanking disc. These are meant to prevent transmission of the high intensity
light to the microscope through unused filter positions. This is primarily a safety feature
that reduces the probability that the operator will look into the optical pathway when
dangerous, high intensity, unfiltered light is present and magnified. A blanking disc in a
filter cup can also function as a shutter in an experimental protocol.

Figure 2-4. Filter holder components.

LAMBDA 10-B OPERATION MANUAL – REV. 1.20Q (20230227)