Sutter Instrument P-30 Operation Manual
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Sutter Instrument P-30 Operation Manual

Micropipette puller
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P P P P - - - - 30
Micropipette Puller
Micropipette Puller
Micropipette Puller
Micropipette Puller
Operation Manual
Rev. 3.10 ( 20100120)
Sutter Instrument Company
One Digital Drive
Novato, CA 94949
Voice: 415-883-0128 Web: www.sutter.com
Fax:
415-883-0572 Email: info@sutter.com
30
30
30

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  • Page 1 P P P P - - - - 30 Micropipette Puller Micropipette Puller Micropipette Puller Micropipette Puller Operation Manual Rev. 3.10 ( 20100120) Sutter Instrument Company One Digital Drive Novato, CA 94949 Voice: 415-883-0128 Web: www.sutter.com Fax: 415-883-0572 Email: info@sutter.com...
  • Page 2 No part of this manual may be reproduced, stored in a retrieval system, or transmitted, in any form or by any means, electronic, mechanical, photocopying, microfilming, recording, or otherwise, without written permission from Sutter Instrument Company. P-30 MICROPIPETTE PULLER OPERATION MANUAL – REV. 3.10 (20100120)

  • Page 3: Safety Warning

    DISCLAIMER DISCLAIMER DISCLAIMER The pipette puller Model P-30 is designed for the specific use of creating micropipettes and no other use is recommended. This instrument creates items that should only be used in a laboratory environment for use on animal tissues. It is not intended for use, nor should be used, in human experimentation, or applied to humans in any way.

  • Page 4: On Operation

    Use only with glassware recommended by Sutter Instrument Company in the following section of Use only with glassware recommended by Sutter Instrument Company in the following section of Use only with glassware recommended by Sutter Instrument Company in the following section of Use only with glassware recommended by Sutter Instrument Company in the following section of th th th this manual.

  • Page 5: Table Of Contents

    APPENDIX B. Accessories Accessories ..............................................21 APPENDIX B. APPENDIX B. Accessories Accessories ..............................................21 21 Nichrome filament block assembly (P-30-NFL/M) ................21 P-30 MICROPIPETTE PULLER OPERATION MANUAL – REV. 3.10 (20100120)
  • Page 6 Figure C-1. Fuse holder..........................23 TABLE OF TABLES TABLE OF TABLES TABLE OF TABLES TABLE OF TABLES Table C-1. Mains fuse type and ratings....................23 Table D-1. Mains fuse type and ratings....................25 P-30 MICROPIPETTE PULLER OPERATION MANUAL – REV. 3.10 (20100120)

  • Page 7: Introduction Introduction Introduction

    The P-30 Micropipette Puller is a derivative of a vertical puller design initially developed at the National Institutes of Health in the late 1950’s. The P-30 is designed to be a basic, economical and simple to operate puller for those investigations where tip diameters down to 0.3 micron and moderate taper lengths are satisfactory.
  • Page 8 (This page intentionally left blank.) P-30 MICROPIPETTE PULLER OPERATION MANUAL – REV. 3.10 (20100120)

  • Page 9: Product Description Product Description

    Catalog Catalog The Model P-30 is shipped to you in a prefabricated foam mold. Please take note of this method of packaging. Should it ever be necessary to ship the puller to another location, the same method of packaging should be employed. Additional packing material may be purchased from Sutter Instrument Company.

  • Page 10: Mechanical Description (Puller Anatomy)

    Mechanical Description (Puller Anatomy) Mechanical Description (Puller Anatomy) This section presents a basic mechanical description of the P-30, with particular emphasis on terminology. Knowing the names of the various parts greatly facilitates communication between the investigators and the manufacturer when discussing adjustments or service problems.

  • Page 11: Pull Solenoid

    Patch Block ( Figure Figure The patch block is a metal block with screws on the top and bottom sides. The patch block provides two adjustable spacers used in forming patch pipettes. P-30 MICROPIPETTE PULLER OPERATION MANUAL – REV. 3.10 (20100120)

  • Page 12: Figure 2-1. P-30 Mechanical Assembly

    Figure 2-1. P-30 Mechanical Assembly. P-30 MICROPIPETTE PULLER OPERATION MANUAL – REV. 3.10 (20100120)

  • Page 13: Installation Installation Installation

    Make certain that the Power Switch located on the front panel of the P-30 cabinet is turned OFF. Plug the power cord provided with the P-30 into the Line Input socket on the Power Entry Module and then to a power source of the appropriate voltage and frequency.
  • Page 14 (This page intentionally left blank.) P-30 MICROPIPETTE PULLER OPERATION MANUAL – REV. 3.10 (20100120)

  • Page 15: First Time Use

    General Operation General Operation The P-30 is quite simple to operate. However, common sense indicates that an instrument that can melt glass must be used with some caution and a complete understanding of its controls. Do not leave the puller powered on and unattended; particularly while a pull- sequence is in progress.

  • Page 16: Multicycle Pulling: Pulling A Patch-Type Micropipette

    (> 1 micron). To construct this type of pipette, it is desirable to process the glass in multiple heating cycles. The P-30 can be used for two-step pipette fabrication with the use of the dual heat settings and the supplied Patch Block. The first of the two steps draws the glass out without separating.

  • Page 17: Patch Block (Spacer)

    Tighten the upper glass clamp while the lower glass clamp rests on the head of the screw. Remove the Patch Block. Set the HEAT switch to HEAT 2, and press PULL. The pipette should separate during this cycle, and the heat will automatically turn off. P-30 MICROPIPETTE PULLER OPERATION MANUAL – REV. 3.10 (20100120)

  • Page 18: Controls Adjustment

    The general rule-of-thumb is that both an increase in the heat and an increase in the pull strength will produce smaller tips and longer tapers. P-30 MICROPIPETTE PULLER OPERATION MANUAL – REV. 3.10 (20100120)
  • Page 19 750 for 3mm platinum filament) and a pull setting above 950. The setting of the optical switch is more complicated. Your P-30 optical switch was set at 2.5 to produce fine micropipettes with 1mm O.D. x .5mm I.D. borosilicate glass when it was shipped to you.
  • Page 20 (This page intentionally left blank.) P-30 MICROPIPETTE PULLER OPERATION MANUAL – REV. 3.10 (20100120)

  • Page 21: Maintenance Maintenance

    HEAT setting to produce the same heat output. Platinum trough filaments for the P-30 are available in three widths: 1.5mm, 2.0m and 3.0mm. Heat delivery to the glass is partially a function of filament width. Thus, both the length of an electrode and the tip diameter will be affected by the filament width.

  • Page 22: Rubber Pads On Clamps

    We have provided this section as an aid for troubleshooting some of the common electronic problems of the P-30. Discussions of circuit board level troubleshooting and circuit board schematics have intentionally been left out. Any work done with the cabinet cover removed requires very careful attention to the hazardous voltages that exist inside cabinet, and should therefore be performed by qualified service personnel only.
  • Page 23 If the heat does not turn off when the optical switch is interrupted, suspect the optical switch. If the heat does turn off, but the pull never turns on, the logic and constant current circuits need to be checked. Please contact Sutter if this is required. P-30 MICROPIPETTE PULLER OPERATION MANUAL – REV. 3.10 (20100120)
  • Page 24 (This page intentionally left blank.) P-30 MICROPIPETTE PULLER OPERATION MANUAL – REV. 3.10 (20100120)

  • Page 25: Appendix A. Appendix A. Limited Warranty Appendix A. Limited Warranty

    LIMITED WARRANTY LIMITED WARRANTY Sutter Instrument Company, a division of Sutter Instrument Corporation, limits the warranty on this instrument to repair or replacement of defective components for one year after the date of shipment, provided the instrument has been operated in accordance with the instructions outlined in the instruction manual.
  • Page 26 (This page intentionally blank.) P-30 MICROPIPETTE PULLER OPERATION MANUAL – REV. 3.10 (20100120)

  • Page 27: Appendix B. Accessories Appendix B. Appendix B. Accessories

    APPENDIX B. APPENDIX B. ACCESSORIES ACCESSORIES The following accessories are available for the P-30 Micropipette Puller. See http://www.sutter.com for more detail and availability P-30 accessories, and for part numbers not expressed in the following list. Nichrome filament block assembly (P...
  • Page 28 (This page intentionally blank.) P-30 MICROPIPETTE PULLER OPERATION MANUAL – REV. 3.10 (20100120)

  • Page 29: Fuse Replacement

    The fuse is located in the fuse holder on the power entry module on the back of the P-30. To remove the fuse holder first unplug the power cord from the power entry module. This will reveal a slot just under the edge of the fuse holder.
  • Page 30 (This page intentionally blank.) P-30 MICROPIPETTE PULLER OPERATION MANUAL – REV. 3.10 (20100120)

  • Page 31: Technical Specifications

    Power cord Power cord Power cord Power cord 10A, 250V, with safety ground plug 10A, 250V, with safety ground plug 10A, 250V, with safety ground plug 10A, 250V, with safety ground plug P-30 MICROPIPETTE PULLER OPERATION MANUAL – REV. 3.10 (20100120)
  • Page 32 (This page intentionally left blank.) P-30 MICROPIPETTE PULLER OPERATION MANUAL – REV. 3.10 (20100120)

  • Page 33: Index

    K K K K pulling a micropipette ........11 keys trip point micrometer........12 PULL ..............5 P P P P reset...............5 power L L L L consumption............29 line power (mains) ...........9 power cord mains..............29 P-30 MICROPIPETTE PULLER OPERATION MANUAL – REV. 3.10 (20100120)
  • Page 34 ........ iii voltage selector switch..........9 slide assembly ............6 W W W W T T T T warranty ..............21 technical specifications..........29 weight ..............29 technical support .............1 P-30 MICROPIPETTE PULLER OPERATION MANUAL – REV. 3.10 (20100120)
  • Page 35 NOTES NOTES NOTES NOTES P-30 MICROPIPETTE PULLER OPERATION MANUAL – REV. 3.10 (20100120)
  • Page 36 NOTES NOTES NOTES NOTES P-30 MICROPIPETTE PULLER OPERATION MANUAL – REV. 3.10 (20100120)

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