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- Page 1 XenoWorks ® Motorized Micromanipulator System (TouchDeclutch) Operation Manual Rev. 4.10 ( 20141208) Sutter Instrument Company One Digital Drive Novato, CA 94949 Voice: 415-883-0128 Web: www.sutter.com Fax: 415-883-0572 Email: info@sutter.com...
- Page 2 Copyright © 2015 Sutter Instrument Company. All Rights Reserved. XenoWorks is a registered trademark of Sutter Instrument Company. ® XENOWORKS MICROMANIPULATOR SYSTEM (TOUCHDECLUTCH) OPERATION MANUAL – REV. 4.10 (20141208)
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Page 5: Disclaimer
SAFETY WARNINGS AND PRECAUTIONS Electrical Operate the XenoWorks BRM using 110-120 V AC, 60 Hz, or 220-240 V AC., 50 Hz line voltage. This instrument is designed for connection to a standard laboratory power outlet (Overvoltage Category II), and because it is a microprocessor--controlled device, it should be accorded the same system wiring precautions as any 'computer type' system. -
Page 6: Electromagnetic Interference
To prevent fire or shock hazard do not expose the unit to rain or moisture. Electromagnetic Interference To comply with FDA and CE electromagnetic immunity and interference standards; and to reduce the electromagnetic coupling between this and other equipment in your lab always use the type and length of interconnect cables provided with the unit for the interconnection of the controller with the manipulator mechanical and with the joystick (see the TECHNICAL SPECIFICATIONS appendix for more details). -
Page 7: Other
Failure to do this may result in damage to the motors. This instrument contains no user-serviceable components — do not open the instrument casing. This instrument should be serviced and repaired only by Sutter Instrument or an authorized Sutter Instrument servicing agent. - Page 8 (This page intentionally left blank.) XENOWORKS MICROMANIPULATOR SYSTEM (TOUCHDECLUTCH) OPERATION MANUAL – REV. 4.10 20141208...
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Page 9: Table Of Contents
TABLE OF CONTENTS DISCLAIMER ............................iii SAFETY WARNINGS AND PRECAUTIONS ..................iii Electrical ..............................iii Avoiding Electrical Shock and Fire-related Injury................iii Electromagnetic Interference .......................iv Operational .............................iv Other ................................v Handling Micropipettes..........................v 1. GENERAL INFORMATION......................1 1.1 Introduction............................1 1.2 About This Manual...........................1 1.3 Technical Support..........................1 1.4 Product Description..........................1 1.4.1 Packing List ..........................1 1.4.2 Joystick Controls and Features ....................2 1.4.2.1 X- and Y-Axis Control ......................3... - Page 10 viii 3.1.6 Cleaning the Micropipette.......................17 3.1.7 Changing the Working Range of Movement.................17 3.1.7.1 Setting a ‘Z-Floor’......................17 3.1.8 Exchanging the Micropipette....................18 3.2 Starting a Microinjection Session ....................18 3.2.1 Setting up the Microscope.......................18 3.2.2 Setting up the Micromanipulator...................18 4. MAINTENANCE..........................21 4.1 General.............................21 4.2 Troubleshooting ..........................21 4.3 Transporting the Micromanipulator ....................25 APPENDIX A.
- Page 11 Table C-1. Mains fuse type and ratings....................31 Table D-1. Speed settings and ranges of movement................33 Table D-2. Mains fuse type and ratings....................34 Table D-3. XenoWorks BRM Controller cables and receptacles/connectors........34 XENOWORKS MICROMANIPULATOR SYSTEM (TOUCHDECLUTCH) OPERATION MANUAL – REV. 4.10 20141208...
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Page 13: General Information
Support staff with your suggestions (415-883-0128 or info@sutter.com). 1.3 Technical Support Unlimited technical support is provided by Sutter Instrument Company at no charge to our customers. Our technical support staff is available between the hours of 8:00 AM and 5:00 PM (Pacific Time) at (415) 883-0128. -
Page 14: Joystick Controls And Features
Figure 1-1. Components of the XenoWorks micromanipulator. 1.4.2 Joystick Controls and Features The user interface of the XenoWorks™ Micromanipulator is a three-axis joystick. When the micromanipulator is installed correctly, the joystick oriented appropriately on the working surface, and the default axis polarity settings used (see “Axis Polarity”), a displacement of the joystick to the left, for example, will result in concomitant movement of the micromanipulator to the left. -
Page 15: X- And Y-Axis Control
Figure 1-2. Joystick. Figure 1-3. The rear panel of the joystick. 1.4.2.1 X- and Y-Axis Control Movements of the micromanipulator in the X- and Y-axes are achieved by moving the joystick left and right, and forward and backward, respectively (the switches for establishing axis polarity for right- and left-handed manipulator mechanicals are located on the back of the joystick). -
Page 16: Y-Axis On/Off
1.4.2.2 Y-Axis On/Off The Y-axis can be turned off to produce pure X-axis travel. This results in a mode that may be preferred for difficult injections/transfers, or when the cell or egg is more sensitive to the withdrawal of the pipette. 1.4.2.3 Z-Axis Control Movement of the micromanipulator in the Z-axis (vertical) is achieved by rotating the control on the end of the joystick. -
Page 17: Work' Position
Although there is no recommended setting for this friction, it should be noted that if there is no friction at all, the joystick would always fall to the center position under gravity, which may not be desirable. Similarly, if the friction is set too tightly, the joystick may become too stiff to use or may be locked in place and could be damaged if the dial is tightened further. -
Page 18: Swing Gate
1.4.2.12 Swing Gate The swing gate on the front of the mechanical allows the micropipette to be swung out toward the front of the microscope for better access when changing micropipettes. The gate can be released by loosening the fixing screw. The screw should be tightened fully during use. Figure 1-5. -
Page 19: Axis Polarity
1.4.3.2 Axis Polarity Occasionally, it may be desirable to reverse the direction of one, two or all three motors so that the movement of the joystick results in movement of the corresponding manipulator mechanical in the opposite direction. An example of this would be the use of the manipulator with an upright microscope where the image is inverted. - Page 20 (This page intentionally left blank.) XENOWORKS MICROMANIPULATOR SYSTEM (TOUCHDECLUTCH) OPERATION MANUAL – REV. 4.10 (20141208)
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Page 21: Installation
2. INSTALLATION The directions below describe the installation procedure common to both left- and right- handed micromanipulators, although the figures illustrate a right-hand micromanipulator (a left hand micromanipulator would be illustrated as a mirror image of these figures). It is extremely important that these installation instructions are followed closely. -
Page 22: Figure 2-2. Engaging The Manipulator Base Plate In The Microscope Adaptor
5. Remove the six (6) shipping screws (indicated by the red stripes) IMPORTANT: Retain the six (6) shipping screws and keep them with the instrument packaging in the event that the instrument needs to be shipped to another location. 6. Carefully place the manipulator on the base plate so that the micropipette holder clamp faces toward the front of the microscope, as shown above for a right-hand micromanipulator. -
Page 23: Table A-1. Mains Fuse Type And Ratings
Table A-1. Mains fuse type and ratings. Mains Voltage Fuse Setting (Type: Time Delay, 5mm x 20mm, glass tube) Rating Manufacturer Examples “110” 2A, 250V Bussmann: GMC-2A, GMC-2-R (RoHS), GDC-2A, or S506- (100 – 120 (Time 2A (RoHS) VAC) Delay) Littelfuse: 239 002 or 239.002.P (RoHS) “220”... - Page 24 See the next section for details on how to align a micropipette at the start of an injecting session. XENOWORKS MICROMANIPULATOR SYSTEM (TOUCHDECLUTCH) OPERATION MANUAL – REV. 4.10 (20141208)
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Page 25: Operating Instructions
3. OPERATING INSTRUCTIONS 3.1 General 3.1.1 Moving the Micropipette with the Joystick Once the micromanipulator has been installed correctly, the micropipette should follow the movement of the joystick. The higher the number on the range control, the smaller the movement will be for any given deflection of the joystick. When in range settings ‘2’ through ‘5’, the micropipette will follow the movements of the joystick without any delay. -
Page 26: Setting The Angle Of Approach Of The Micropipette
3.1.3 Setting the Angle of Approach of the Micropipette The micropipette holder clamp is capable of rotating around its own axis and the movement can be locked with the thumbscrew (1) shown here. It is also possible to slide the clamp and lock it in place with a separate thumbscrew (2). -
Page 27: Figure 3-3 (A And B). Suggested Approach For Suspended Cells With Angled Micropipettes (Icsi, Stem Cell, Nuclear Transfer)
Setup for various applications Figure 3-3 (a and b). Suggested approach for suspended cells with angled micropipettes (ICSI, Stem Cell, Nuclear Transfer). Figure 3-4 (a and b). Suggested approach for suspended cells with straight micropipettes (Pronuclear Injection). Figure 3-5 (a and b). Suggested approach for adherent cells rand straight pipettes. XENOWORKS MICROMANIPULATOR SYSTEM (TOUCHDECLUTCH) OPERATION MANUAL –... -
Page 28: Micropipette Type
3.1.3.3 Micropipette Type Some micropipettes (lab-made or purchased ICSI pipettes, for example) are often designed with an angle close to the tip. This is to allow the micropipette tip to approach the injected cell from a trajectory parallel to the bottom of the chamber, and to allow the use of a high- sided dish. -
Page 29: Cleaning The Micropipette
position. Briefly pressing the ‘Work’ key will reverse the direction and move the micropipette to the ‘Work’ position (In this way, a micropipette can be partially raised out of the injection chamber and returned, without having to move all the way out to the ‘Home’ position – see “Cleaning the Micropipette”... -
Page 30: Exchanging The Micropipette
3.1.8 Exchanging the Micropipette During the course of a microinjection procedure, it may be necessary to exchange micropipettes. One effective method of doing this is to incorporate the ‘Home’ function in the following manner: 1. Center the micropipette in the microscope field of view and above the dish by 3 mm, and press and hold down the ‘Work’... - Page 31 3. Load a micropipette (appropriate to the chosen application) into the micropipette holder and install it in the clamp of the mechanical so that the tip of the micropipette is pointing in and down towards the optical axis of the microscope. 4.
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Page 33: Maintenance
4. MAINTENANCE 4.1 General The XenoWorks Micromanipulator requires no regular maintenance. In order to avoid restricted movement of the mechanical, make sure that all cables are not taught and have freedom of movement. Do not oil the mechanicals. If removed for storage or shipping, press the Setup button on the back of the joystick before power down so that the shipping screw holes are in alignment. - Page 34 Problem Cause Solution 4. The O-rings in the The micropipette holder has micropipette holder been designed to minimize chuck are missing or vibration when used with damaged. three O-rings in the chuck, and when the O-rings are the only thing contacting the back end of the micropipette.
- Page 35 Problem Cause Solution 4. The swing gate Tighten the swing gate thumbscrew is loose. thumbscrew. 5. The mechanical is not Ensure all four screws fixing screwed securely to the the mechanical to its base mechanical base plate. plate are tight. 6.
- Page 36 Problem Cause Solution One or more motors move One or more of the axis Flip the appropriate DIP in the opposite direction to polarity DIP switches are in switches (see the axis guide in that commanded by the the wrong position. section 2.4).
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Page 37: Transporting The Micromanipulator
Problem Cause Solution 3. The joystick was Press ‘Work’ again. inadvertently moved during the automatic return to the ‘Work’ position. The movements of the The domestic voltage supply Investigate the domestic mechanical do not may have dropped below the power supply for your facility. correspond with the specification for the It may be necessary to install... - Page 38 8. Locate the shipping screw holes. There are four through-holes in each axis slide, but only two of the holes will line up with the correct tapped holes on the inner slide surface when the instrument is in the ‘Setup’ position. 9.
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Page 39: Appendix A. Limited Warranty
Warranty work will be performed only at the factory. The cost of shipment both ways is paid for by Sutter Instrument during the first three months this warranty is in effect, after which the cost is the responsibility of the customer. - Page 40 (This page intentionally blank.) XENOWORKS MICROMANIPULATOR SYSTEM (TOUCHDECLUTCH) OPERATION MANUAL – REV. 4.10 (20141208)
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Page 41: Appendix B. Accessories
APPENDIX B. ACCESSORIES The following accessories are available for the XenoWorks Micromanipulator system. Extensions 4-in (10.16-cm) Dovetail Extension (X285204) Z-axis vertical extension (X285305) Z-axis horizontal extension (X285310) Rotating Base Rotating base (225RBI) Mounts Hinged headstage mount (285HEA) Adapters For use with MT and MD series stands and Mounting adapter plate (X285210). - Page 42 (This page intentionally blank.) XENOWORKS MICROMANIPULATOR SYSTEM (TOUCHDECLUTCH) OPERATION MANUAL – REV. 4.10 (20141208)
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Page 43: Appendix C. Fuse Replacement
Replace the active fuse with the spare and reinstall the fuse holder and power cord. If the micromanipulator fails to power up with the new fuse installed, call Sutter Instrument technical support for assistance. Table C-1. Mains fuse type and ratings. - Page 44 (This page intentionally blank.) XENOWORKS MICROMANIPULATOR SYSTEM (TOUCHDECLUTCH) OPERATION MANUAL – REV. 4.10 (20141208)
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Page 45: Appendix D. Technical Specifications
APPENDIX D. TECHNICAL SPECIFICATIONS Manipulator Mechanical: 3-axis stepper motor X, Y, and Z Axes 62.5 nm per microstep 25 mm (1 in) full travel Joystick: Inverted joystick with integral Declutch Height and resistance adjustable Independent axis-polarity inversion 6-position rotary range-of-movement control ‘Setup’... -
Page 46: Table D-2. Mains Fuse Type And Ratings
Littelfuse: 218 001 or 218 001.P (RoHS) VAC) Cables (Refer to the following two tables for a description of all possible cables.) Table D-3. XenoWorks BRM Controller cables and receptacles/connectors. Controller Rear Panel Cable Connector Connects to ... Cable Type... - Page 47 Storage Environment: Temperature: 0 – 70 °C (32 – 158 °F) Humidity: 0 – 95% (non-condensing) Operating Environment: Temperature: 3.5 – 35 °C (38.3 – 95 °F) Humidity: 0 – 80% (non-condensing; 80% @ 31°C (87.8°F) decreasing linearly to 67% @ 35°C (95°F)) Regulatory: Safety - EN61010-1, CE EMC - EN61326, CE...
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Page 49: Index
INDEX spare..............31 fuses, replacement accessories ............. 29 mains............. iii, 31 adapters ............. 29 extensions............29 holders ............... 29 General Information ..........1 mounts............... 29 about this manual..........1 rotating base............29 technical support ..........1 General Safety Information........iii Additional Features and Functions Setup..............6 glassware precautions............v... - Page 50 joystick............... 33 manipulator mechanical ........33 safety warnings............iii ranges of movement ......... 33 safety warnings & precautions storage environment ........35 operational............iv safety warnings & precautions......iv Setting the Angle of Approach of the Micropipette technical specifications......... 33 Applications and Suggested Setups ....14 technical support .............1 Microinjection chamber ........
- Page 51 NOTES XENOWORKS MICROMANIPULATOR SYSTEM (TOUCHDECLUTCH) OPERATION MANUAL – REV. 4.10 20141208...
- Page 52 NOTES XENOWORKS MICROMANIPULATOR SYSTEM (TOUCHDECLUTCH) OPERATION MANUAL – REV. 4.10 (20141208)
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