When To Use The Seal Wash Function - Agilent Technologies InfinityLab LC Series User Manual

4 Optimizing Performance

When to use the Seal Wash Function

When to use the Seal Wash Function

Highly concentrated buffer solutions will reduce the lifetime of the seals and
pistons in your pump. The seal wash function allows to maintain the seal lifetime
by flushing the back side of the seal with a wash solvent.
The seal wash function is strongly recommended when buffer concentrations of
0.1 M or higher will be used for long time periods in the pump.
The seal wash function comprises a support ring, secondary seal, gasket and
seal holder for both piston sides. Place a wash bottle filled with 90 % water / 10 %
isopropanol above the pump in the solvent cabinet and guide the SW waste into
the waste bottle underneath the LC stack. The peristaltic pump moves a flow
through the pump head removing all possible buffer crystals from the back of the
pump seal. This mixture prevents growth of algae or bacteria in the wash bottle
and reduces the surface tension of the water.
Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual 82