Method Parameter Settings - Agilent Technologies InfinityLab LC Series User Manual

3 Using the Pump
Setting up the Pump with the Instrument Control Interface

Method Parameter Settings

The pump method setup parameters are in eight sections:
•
•
•
•
•
•
•
Table 12 Method parameters
Parameter Limits
Flow ≤200 bar: 0.0 – 10.00 mL/min in
steps of 0.001
> 200 bar: 0.0 – 5.00 mL/min in
steps of 0.001.
Solvent(s)
Stoptime 0.01 – 99999 minor As
Injector/No Limit
(an infinite run time).
Posttime 0.01 – 99999 minor Off
(0.0 min).
Pressure Max: 600 bar (8700 psi) for flow
Limits rates up to 5 mL/min. 200 bar
(2900 psi) for flow rates
> 5 mL/min.
Min: any value between 0 and
the upper pressure limit setting.
Timetable
Advanced
Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual
Flow
Solvent(s)
Stoptime
Posttime
Pressure Limits
Timetable
Advanced
Description
The flow is the rate of movement of eluent along the column. It is
important that the flow rate is kept constant to ensure precise retention
time, and peak measurements. Variations in flow rate can occur as a
result of the following: partial failure of the pumping system, air in the
pumping system, a change in the mobile phase viscosity, or a
temperature change.
Isocratic Pump: The text box allows you to type a brief description of the
solvent.
Quaternary Pump: You can set the percentages of solvents B, C, and D to
any value from 0 through 100 %. Solvent A always delivers the remaining
volume: 100 - (%B + %C + %D). The check boxes allow you to turn on the
solvent channels (checked) or off (cleared). The text boxes allow you to
type a brief description of each of the solvents.
The stoptime sets a time limit for your analysis. After the stoptime, all
gradients are stopped and the pump parameters return to their initial
values. The pump can be used as a stoptime master for the complete
analytical system. The pump also stops the detectors if they have a No
Limit stoptime setting. If no limit is given, a method will have to be
stopped manually.
Your instrument remains in a not ready state during the posttime to delay
the start of the next analysis. You can use the Posttime to allow your
column to equilibrate after changes in solvent composition (for example
after gradient elution).
Sets the maximum and minimum pressure limits for the pump.
• Max is the maximum pressure limit at which the pump will switch
itself off, protecting the analytical system against overpressure.
• Min is the minimum limit at which the pump will switch itself off. For
example, if any solvent reservoir is empty, this setting prevents
system damage by pumping air.
See
See
"Timetable Settings" on page 64
"Advanced Settings" on page 63
62