Equilibrating The System - Thermo Scientific Dionex UltiMate 3000 Series Operating Instructions Manual

UltiMate 3000 Series:
LPG-3400XRS Pump

4.7 Equilibrating the System

Before using the pump for sample analysis, equilibrate the UltiMate 3000 system:
1. Pump the starting solvent through the entire system until the system is free of any
other liquid composition.
2. Heat or cool all temperature-controlled devices to the temperature required for the
application.
3. Set the detector wavelengths and turn on the lamps.
4. Monitor the pump pressure. Verify that the reading is correct for the application and is
stable.
5. Monitor the detector signal and verify that the baseline signal is at the expected
reading for your application and is stable.
Perform system equilibration in Chromeleon or select the required commands and
parameters on the front panel menus of the modules.
To equilibrate the system from Chromeleon
Select and perform the operating commands and parameters from the Commands dialog
•
box.
• Create and run an equilibration program to automate the process (→ page 72).
Page 66 Operating Instructions