General Notes - Thermo Scientific Dionex UltiMate 3000 Series Operating Instructions Manual

4.3.1 General Notes

When connecting the solvent reservoirs, observe the following general precautions:
Before first use, rinse solvent bottles thoroughly using highly pure solvents.
•
• Always use the original solvent lines. This prevents contaminants from reaching the
HPLC system that may increase wear and cause damage to the system.
• Regularly check the filter frits for permeability. This is especially important when using
aqueous solvents. Aqueous solvents may contaminate the filters with algae and other
microorganisms that deposit on the filter frits. Therefore, use fresh solvents at regular
intervals. Clean the reservoirs thoroughly before filling them. Replace the filter frits as
necessary.
• Be sure to use the solvent supply lines with the appropriate filter frits. As a standard,
solvent lines with stainless steel frits are provided in the accessories kit of the pump.
• Make sure that the tubing connecting the pump to the degasser is as short as possible and
locate the solvent reservoirs as close as possible to the pump. To avoid formation of air
bubbles in the reservoirs and reformation of air bubbles in the solvent, make sure that the
reservoirs are on the same level or higher as the pump.
• Before connecting the solvent supply lines, make sure that the connectors are free of
contaminants. Even minute particles can allow air to enter the degasser, and thus reduce
the degassing effectiveness.
• Normal phase eluents usually show only a low concentration of dissolved gases.
Therefore, it is normally not required to use a degasser with these eluents.
Keep the following in mind:
When the fluid components of the pump are filled with liquid and the solvent reservoirs are
located above the pump outlet during pump operation, the hydrostatic pressure in the
system may cause eluent to escape when you open a fluid connection in the pump. Before
you open a fluid connection, position the reservoirs below the connection to be opened.
Operating Instructions
UltiMate 3000 Series:
LPG-3400XRS Pump
Page 59