3DHISTECH PANNORAMIC SCAN RX 1.1 User Manual page 83

PANNORAMIC® SCAN RX 1.1
o
Pre-scan + high-resolution – creates a High-Resolution fast FL preview image of
every FOV within the selected area (in single layer DAPI, with an illumination time
of 20ms) that is displayed in the Profile Wizard window. Scanning process is
initiated with a simple Pre-scan, the located sample with auto threshold is
selected, and finally, a Pre-scan in high resolution is launched.
•
Thresholding
The manually set threshold value is applied during scanning. The preview of the loaded
slide is displayed in the Preview window. The set value has effect on the preview image. If
threshold value is modified in the Microscope Controller preview window, that value is
automatically applied in the profile in the Profile Settings panel.
o Specimen threshold
o
Manually set value: This sets the sensitivity of the specimen detection. To set the
desired threshold value manually, change the setting in the Specimen threshold
level selection box in the range of 0-255. The default value is 212. Smaller values
result in a more sensitive scanning, meaning that more areas will be digitized,
however, this may also include specks or other imperfections of the slide. Greater
values result in a less sensitive scanning, but it is possible that some of the smaller
specimen areas will not be digitized.
o
Auto Threshold
By clicking
image.
Caution!
Make sure that the detection result is acceptable for you. Otherwise, set the threshold value
manually or append missing areas with the help of appropriate functions in the Preview
window.
o
Use marker with threshold
If this option is selected, only the area encircled with a marker pen will be
scanned. If the specimen cannot be identified for digitization, (for example,
contrast is weak), encircling the specimen with a black marker pen can be of help
before running the detection. You can define the threshold level by changing the
threshold value in the Use marker with threshold selection box in the range of 0-
255. This sets the sensitivity of the specimen detection inside the marker area.
To set the desired threshold value manually, change the setting in the Specimen
threshold selection box in the range of 0-255. The default value is 105.
NOVEMBER 18, 2021
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
, the specimen will automatically be detected in the preview
SCANNING MODES AND SETTINGS
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