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3DHISTECH Pannoramic 250 FLASH III User Manual

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Pannoramic 250 FLASH III
3.0.2
User's Guide
Sep 16, 2020
Rev. 1
P250FIII302-UG-Rev1

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Summary of Contents for 3DHISTECH Pannoramic 250 FLASH III

  • Page 1 Pannoramic 250 FLASH III 3.0.2 User’s Guide Sep 16, 2020 Rev. 1 P250FIII302-UG-Rev1...

  • Page 2: Table Of Contents

    Pannoramic 250 FLASH III 3.0.2 User’s Guide Contents Disclaimer........................5 Character Formats and Symbols..................7 Declaration of Conformity....................8 About this User's Guide....................9 Notes Regarding Operational Safety................10 Notes on Warranty......................12 Terms and Abbreviations....................13 1 Product Description....................14 1.1 Product Overview........................14...
  • Page 3 Pannoramic 250 FLASH III 3.0.2 User’s Guide 4.1.2 Exiting the software............................39 4.2 Main window functions......................39 5 Scanning modes and settings..................56 5.1 Brightfield Scanning Mode......................56 5.1.1 Profile Settings Panel.............................57 5.1.2 Microscope Control Panel..........................64 5.1.3 Quick preview functions..........................75 5.1.4 Quick microscope functions..........................75 5.2 Fluorescence Microscope Mode.....................77...
  • Page 4 Pannoramic 250 FLASH III 3.0.2 User’s Guide 7.4.5 Removing a Magazine from the Transporting System.................147 7.4.6 Transporting..............................149 8 Technical Data......................151 Index........................... 156 September 16, 2020 – Rev. 1 3DHISTECH Ltd. 4(156)

  • Page 5: Disclaimer

    IP are the sole properties of the 3DHISTECH Ltd. 3DHISTECH Ltd. is not liable for damage of whatever nature (including, but not limited to, general or specific damage, indirect damage, consequential damage or incidental damage, including the...
  • Page 6 THE SOFTWARE IS PROVIDED “AS IS” AND WITHOUT WARRANTY OF ANY KIND, EXPRESSED, IMPLIED OR OTHERWISE, INCLUDING WITHOUT LIMITATION, ANY WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. Further Information For the latest information on 3DHISTECH products and services, please visit our website at the following URL: http ://www.3dhistech.com Company Address 3 Öv Street, 1141 Budapest –...

  • Page 7: Character Formats And Symbols

    Pannoramic 250 FLASH III 3.0.2 User’s Guide Character Formats and Symbols Example Words or characters that appear on the screen. These include field names, screen and window titles, push-buttons and menu names, paths or options. Keys on the keyboard. For example, function keys (such as F11) or the Ctrl+M key combination.

  • Page 8: Declaration Of Conformity

    Pannoramic 250 FLASH III 3.0.2 User’s Guide Declaration of Conformity 3DHISTECH Ltd. declares that the product Pannoramic® 250 FLASH III slide scanner is designed and produced with consideration of specified requirements according to the IEC 61326-2-6:2012 standard, and conforms to the directives and other standards listed below: IEC 61010-1:2010, “Safety Requirements for Electrical Equipment for Measurement, Control, and...

  • Page 9: About This User's Guide

    Pannoramic 250 FLASH III 3.0.2 User’s Guide About this User's Guide About this User's Guide The purpose of this manual is to assist the user in the safe and reliable operation of the product described. Before operating the device, you must carefully read this instruction manual, and adhere...

  • Page 10: Notes Regarding Operational Safety

    Pannoramic 250 FLASH III 3.0.2 User’s Guide About this User's Guide Notes Regarding Operational Safety The below section of this User’s Guide contains information and warnings of a kind that must be followed by owner/operator personnel. Warning and advisory symbols which are used on the base unit of the Pannoramic® 250 FLASH III and in chapter 1.1.3 Warning and Information Labels have the following meanings:...
  • Page 11 Pannoramic 250 FLASH III 3.0.2 User’s Guide About this User's Guide Remember to unplug the power cord before you open the Pannoramic® 250 Disconnect plug! FLASH III or replace a fuse! Use only electric fuses which are rated for the specified nominal current. You Warning! are prohibited from using makeshift fuses or short-circuiting of a fuse holder.

  • Page 12: Notes On Warranty

    About this User's Guide Notes on Warranty 3DHISTECH Ltd. – as the Product Manufacturer – warrants the Pannoramic® 250 FLASH III to be free from faults in material and workmanship at the moment of installation. Defects must be notified immediately on identification and maximum efforts must be undertaken in order to the amount of damage as small as possible.

  • Page 13: Terms And Abbreviations

    Pannoramic 250 FLASH III 3.0.2 User’s Guide About this User's Guide Terms and Abbreviations Comma Separated Value Field of View Numerical Aperture Photobleaching A phenomenon that leads to a dramatic loss of fluorescence emission intensity (a fluorophore permanently loses the ability to fluoresce) due to photon-induced chemical damage and covalent modification, therefore fluorescent signals cannot be properly detected.

  • Page 14: Product Description

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 1 Product Description 1 Product Description 1.1 Product Overview 1.1.1 Intended Use Pannoramic® 250 FLASH III is designed to use transmitted light and reflected light fluorescence to digitize biological samples and specimens (typically histological sections). The target users of the product can work for example, in education, research, industry or medical sectors.
  • Page 15 Pannoramic 250 FLASH III 3.0.2 User’s Guide 1.1.2 Features and Benefits Fluorescent scanning mode: • High-end fluorescent illumination device – Lumencor SPECTRA Light Engine 6 • 3 times faster and more silent filter change • Multiband filter support • FL scanning with a low-noise, 16-bit scientific CMOS pco.edge 4.2 camera / Back •...

  • Page 16: Warning And Information Labels

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 1.1.3 Warning and Information Labels 1.1.3 Warning and Information Labels The following figure contains the labels used on the Pannoramic® 250 FLASH III main unit. Figure 1 – Warning and information labels NOTE: The symbol at the magazine feeder refers to the possibility of exposure to biological hazards whenever handling slides.

  • Page 17: Main Parts Of The Base Unit

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 1.1.4 Main parts of the Base Unit 1.1.4 Main parts of the Base Unit The following figures show the main parts of the base unit. Figure 2 – Main parts of base unit 1.

  • Page 18: System Overview

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 1.2 System Overview 1.2 System Overview The standard scope of delivery of the product includes the following hardware and software components. 1.2.1 Pannoramic® 250 FLASH III system The following figure shows the main parts of the Pannoramic® 250 FLASH III system.

  • Page 19: Hardware Options

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 1.2.2 Hardware Options 1.2.2 Hardware Options The configuration of the control computer and monitor can vary depending on the product order. Cameras Adimec QUARTZ Q-12A180 1 CMOS camera for high-quality brightfield applications allowing ultra-fast scanning up to 180 fps.
  • Page 20 Pannoramic 250 FLASH III 3.0.2 User’s Guide 1.2.2 Hardware Options 0.63× adapter 1x adapter 1.6x adapter (for Point Grey Grasshopper (for Fluorescent only) (for Brightfield only) 3 camera only) Motorized camera changer Figure 4 – Motorized camera changer You can fit two cameras into the Pannoramic® 250 FLASH III on the first adapter (1) (fluorescent position) and the second adapter (2) (brightfield position) at the same time.
  • Page 21 Pannoramic 250 FLASH III 3.0.2 User’s Guide 1.2.2 Hardware Options Objectives Figure 5 – Objective changer You can select two different objectives to be equipped with the Pannoramic® 250 FLASH III, the first objective (1) and the second objective (2).
  • Page 22 NOTE: If Lumencor SPECTRA 6 unit is ordered, the custom light source and filter modules can be variable. For more information, please contact 3DHISTECH customer service. Objectives Plan-Apochromat 10×/NA 0.45 (optional, can be used only for FL scanning) •...

  • Page 23: Control Software

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 1.2.2 Hardware Options Warning! Do not change correction collar settings on 40x objective, if you need it to be set, contact customer service. Fluorescent Option for Pannoramic® 250 FLASH III Fluorescent unit (internal part)

  • Page 24: Installation

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 2 Installation 2 Installation Warning! The installation of Pannoramic® 250 FLASH III must be carried out by trained professionals! In any other case the warranty will be automatically voided. Supplier/Distributor is not liable for any damages caused by an installation that is not carried out by a professional person.
  • Page 25 Pannoramic 250 FLASH III 3.0.2 User’s Guide 2.1 Operation system of the control computer Third-party antivirus programs can affect the operation of the software, but we are not responsible for any resulting problems. We recommend that you place the following (default) folders in the "real- time scanning"...

  • Page 26: Control Computer Ports

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 2.2 Control computer ports 2.2 Control computer ports The following figure shows the location of the connectors in order to enable easier transportation of the product for a short distance, by disconnecting cables and detaching hardware components from each other.

  • Page 27: Connectors And Cables

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 2.3 Connectors and cables 2.3 Connectors and cables Control – USB (between scanner and control computer) • Preview camera – USB cable (between scanner and control computer) • Adimec – CoaXPress cables (between Adimec camera and control computer) •...
  • Page 28 Pannoramic 250 FLASH III 3.0.2 User’s Guide 2.4 Mounting the Lumencor SPECTRA 6 unit 3. Find the mounting hole (1) at the back side of the Pannoramic® 250 FLASH III unit and rotate the Lumencor SPECTRA 6 unit (2) upside down.

  • Page 29: Power The Scanner On/Off

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 2.4 Mounting the Lumencor SPECTRA 6 unit 6. Secure the locking lever by moving it to the left (see Figure 10 – Step 2). If the lever stucks at around the center position, apply a bit more pressure to the Lumencor SPECTRA 6 unit.

  • Page 30: Operating Slides And Magazines

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 3 Operating Slides and Magazines 3 Operating Slides and Magazines 3.1 Preparing Slides and Magazines This section demonstrates how to prepare slides and magazines. You can use all slides and cover slips that meet the following specifications:...
  • Page 31 Pannoramic 250 FLASH III 3.0.2 User’s Guide 3.1 Preparing Slides and Magazines Warning! Ensure that no part of the mounted coverslip protrudes from the edges of the slide. Use slides only with completely dried embedding medium. If not so, the coverslip can be shifted that makes slide loading less reliable.

  • Page 32: Affixing Barcode Stickers To Slides

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 3.2 Affixing Barcode Stickers to Slides 3.2 Affixing Barcode Stickers to Slides This section describes how to affix barcode stickers to slides. Barcodes enable virtual slide identification. Figure 12 – Slide with a barcode sticker The label area (1) is recorded by the preview camera, and can be displayed by the control and viewer software.

  • Page 33: Inserting And Removing Slides

    3.3.1 Insert slides into a magazine Figure 13 – Inserting slides into a magazine Caution! Only original 3DHISTECH P250 magazines are allowed for use with Pannoramic® 250 FLASH III! The magazines can contain a maximum of 25 slides. Push slide (1) into the magazine (3) with its barcode end (2) first. The symbols on the side of •...

  • Page 34: Insert And Remove Magazines

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 3.3.1 Insert slides into a magazine Magazines should always be kept clean from dust, broken glass and other mounting media residues. Slide loader will only operate safely and properly, if magazines are clean.
  • Page 35 Pannoramic 250 FLASH III 3.0.2 User’s Guide 3.3.2 Insert and remove magazines Warning! The protective cover must always be closed while the microscope is loading and/or scanning! Note: The guide rails have built-in sensors that detect whether there are magazines inserted in the input stack, or whether the output stack is full.
  • Page 36 Pannoramic 250 FLASH III 3.0.2 User’s Guide 3.3.2 Insert and remove magazines Caution! Make sure that the magazines are properly placed between the rails, because it can cause serious damages in the feeding mechanism. Figure 16 – Misplaced magazine Note: The input stack can be filled with a maximum of 11 magazines in addition to the one in the magazine loader.

  • Page 37: Opening/Closing The Fl Protective Cover

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 3.3.3 Opening/Closing the FL protective cover 3.3.3 Opening/Closing the FL protective cover Figure 17 – Opening the FL protective cover Open the protective cover by moving it upwards and lay it down to the upper cover of the unit, then insert magazines.

  • Page 38: Software Description

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 4 Software Description 4 Software Description 4.1 Starting / Exiting Pannoramic® Scanner Software 4.1.1 Starting the software To start the program, double-click on the program icon on the desktop or start it from Start \ Program Files \ 3DHISTECH \ Pannoramic Scanner Before software initialization the user interface is opened and the mode selection icons are displayed.

  • Page 39: Exiting The Software

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 4.1.2 Exiting the software 4.1.2 Exiting the software To exit the software, Click X at the main window header to close the program. Note, that when exiting the software, the loaded slide will be automatically unloaded.
  • Page 40 Pannoramic 250 FLASH III 3.0.2 User’s Guide 4.2 Main window functions Slide/magazine control Note: The slide sensor has an optoelectronic gate, and a lever. At the slide loading position, the lever is lifted into the gate and shutters the lightbeam. If there is no slide in the loader arm, the lever cannot be lifted into the gate and this way, the lightbeam is not shuttered.
  • Page 41 Pannoramic 250 FLASH III 3.0.2 User’s Guide 4.2 Main window functions The live image completely fills the display area ◦ Displays or hides the vertical and horizontal scale bars ◦ Displays the magnifier window ◦ Activates red marking on overexposed pixels ◦...
  • Page 42 Pannoramic 250 FLASH III 3.0.2 User’s Guide 4.2 Main window functions – Multi-channel histogram with normalization ◦ – Invert image (for testing purposes) ◦ – Mark overexposed pixels ◦ – Scan Properties Unique settings can be defined even for each separate scanning. This function is included on the profile cards as well, added to the slide in Planner mode.
  • Page 43 Pannoramic 250 FLASH III 3.0.2 User’s Guide 4.2 Main window functions Click at the left of a list item to open the Barcode editor window in which the settings of the selected profile can be edited. If you click Add new item ... on the list, blank input fields will be displayed.
  • Page 44 Pannoramic 250 FLASH III 3.0.2 User’s Guide 4.2 Main window functions Define/modify profile name in the Name input field, select barcode type from • the Type drop-down list. Define/modify ranges within the barcode string when Range is selected at •...
  • Page 45 Pannoramic 250 FLASH III 3.0.2 User’s Guide 4.2 Main window functions Target format: The slide is converted to the selected final format. Click one of the ▪ buttons to define the final slide format (MRXS, Tiled-TIFF, Roche-TIFF, SVS, DICOM). Click to open MRXS, Tiled-TIFF and DICOM conversion settings panel: ▪...
  • Page 46 Pannoramic 250 FLASH III 3.0.2 User’s Guide 4.2 Main window functions Force simple tiff: TIFF files use 32-bit offset addressing, therefore file size ▪ is limited to a maximum of 4Gb. Force big tiff: Big TIFF files use 64-bit offset addressing allowing the ▪...
  • Page 47 Pannoramic 250 FLASH III 3.0.2 User’s Guide 4.2 Main window functions Conversion save path: The folder set in this box is designed to store the final slides ▪ after conversion. Conversion progress is displayed in the Converter queue window of the converter.
  • Page 48 Pannoramic 250 FLASH III 3.0.2 User’s Guide 4.2 Main window functions If the scanning of slide has finished, the progress of Quant evaluation is displayed in the QuantCenter Processing Queue window. For further information on QuantCenter, please read QuantCenter User's Guide.
  • Page 49 Pannoramic 250 FLASH III 3.0.2 User’s Guide 4.2 Main window functions Server settings Select the dedicated server to which you want to scan from the Server drop-down ▪ list. Click the arrow to show the list of saved server profiles, or click Add new item ...
  • Page 50 Pannoramic 250 FLASH III 3.0.2 User’s Guide 4.2 Main window functions Slide naming Barcode parsing: Similarly to the settings of the Scan to local panel, barcode settings ▪ for parsing can be modified in the Barcode Editor window. Figure 22 – Window of Barcode Editor...
  • Page 51 Pannoramic 250 FLASH III 3.0.2 User’s Guide 4.2 Main window functions UPC-A • UPC-E • 2D barcode types Aztec • Data Matrix • PDF417/Compact • Micro PDF417 • • Micro QR • MaxiCode • NOTE: If embedded barcode library (provided by Vintasoft barcode reader software) is used, refer to Figure 98 for the list of supported barcode types.
  • Page 52 Pannoramic 250 FLASH III 3.0.2 User’s Guide 4.2 Main window functions Other settings Scan separately: The FOV areas of the preview image will be scanned and saved ▪ separately as discrete digital slides. – Start Scan Scanning of the actual slide or performing the whole previously planned scanning procedure can be initiated.
  • Page 53 Pannoramic 250 FLASH III 3.0.2 User’s Guide 4.2 Main window functions Whenever you add a new magazine (in Planner mode as well) the software asks you whether the magazine has previously been loaded or not. Click Yes if the magazine has already been processed, then enter the number of the magazine used during the process, otherwise click No.
  • Page 54 Pannoramic 250 FLASH III 3.0.2 User’s Guide 4.2 Main window functions Figure 24 – Profile browser window September 16, 2020 – Rev. 1 3DHISTECH Ltd. 54(156)
  • Page 55 Pannoramic 250 FLASH III 3.0.2 User’s Guide 4.2 Main window functions – Save Profile The selected profile can be saved in this window after typing the profile name into the text field and clicking Save as. – Reset Zoom This function is active only in Live view at 1:1 magnification.

  • Page 56: Scanning Modes And Settings

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 5 Scanning modes and settings 5 Scanning modes and settings After microscope initialization, if fluorescence hardware option is provided, you can select from the following two modes: Brightfield scanning mode (see chapter 5.1 Brightfield Scanning Mode for more information) •...

  • Page 57: Profile Settings Panel

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.1.1 Profile Settings Panel 5.1.1 Profile Settings Panel Objective settings This field displays the objective used for scanning. Objective type: 20x; 40x Focus settings for the scan profile Figure 26 – Focus settings for scan profile Limits of the range: You can define focus limits by selecting Limits of the range for focusing.
  • Page 58 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.1.1 Profile Settings Panel Sample detection This panel offers options for threshold setting and selection of the area to be scanned. Source: Brightfield preview – a normal type of illumination in the microscope for scanning the •...
  • Page 59 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.1.1 Profile Settings Panel If the specimen cannot be identified as required for digitization, (for example, weak contrast), it can help to encircle completely the specimen with a black marker pen. You have to draw a full circle with the pen to enable the detection. Marker pen detection will make specimen detection easier by limiting the scanned area.
  • Page 60 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.1.1 Profile Settings Panel Figure 28 – Preview image with and without coverslip edge Caution! It requires lots of time (since it cannot find focus neither on the edges, nor on specks) to digitize coverslip edges. Select Remove coverslip function to eliminate those areas.
  • Page 61 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.1.1 Profile Settings Panel Load scanmap: This function is useful if the slides contain areas of interest that cannot be • detected by the preview camera. This function along with saving profiles helps to scan all slides with the most adequate settings.
  • Page 62 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.1.1 Profile Settings Panel Flash Z-stack: This function allows the scanning of Z-stack layers with flash illumination. Each • layer is saved as a single slide and are stored in a specific folder. If you use CaseViewer for viewing slides, please bear in mind that the maximum number of parallel windows that can be open are 9, therefore it is advised to set layer count to 9 as well.
  • Page 63 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.1.1 Profile Settings Panel Good (JPEG: 80) • Medium (JPEG: 60) • Low (JPEG: 30) • Custom... • If choosing this option, the Image compression setting is displayed on which you can select from different image formats.

  • Page 64: Microscope Control Panel

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.1.2 Microscope Control Panel 5.1.2 Microscope Control Panel The Microscope Control panel includes the main control features of the microscope. 5.1.2.1 Preview Window The Preview panel offers options for threshold setting and selection of the area to be scanned.
  • Page 65 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.1.2 Microscope Control Panel Preview Type setting determines the type of illumination. In Brightfield mode, the following preview types are available: Brightfield preview – a normal type of illumination in the microscope for scanning the •...
  • Page 66 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.1.2 Microscope Control Panel Note: If a hematology slide is detected including blood smear, the Remove Coverslip function cannot be activated and focusing on the coverslip area is not available. If you want to use the Remove Coverslip function, first switch to Custom Scanmap.
  • Page 67 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.1.2 Microscope Control Panel Use marker with threshold : If the specimen cannot be identified as required for digitization, (for example, weak contrast), it can help to encircle completely the specimen with a black marker pen.
  • Page 68 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.1.2 Microscope Control Panel Preview Toolbar This section is located at the bottom of the preview image. Save scanmap – Click this icon to save the currently selected areas to scanmap. • Position –...
  • Page 69 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.1.2 Microscope Control Panel Crop – Crop the area of the selected area. ◦ Place Circle – You can select the area to be scanned with the different tools on the • Preview toolbar.
  • Page 70 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.1.2 Microscope Control Panel Enter the distance between two spots on the X and the Y axis in µm in the Core distance fields or select the appropriate values with the arrows. Enter the average diameter of the spots in the Core size field or select the appropriate value with the arrows.
  • Page 71 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.1.2 Microscope Control Panel Figure 38 – Colors in preview image and Label area switching button September 16, 2020 – Rev. 1 3DHISTECH Ltd. 71(156)
  • Page 72 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.1.2 Microscope Control Panel Areas that will be scanned These areas are digitized on the basis of user settings (for example, Fill holes in scan area) and not detected by the selected threshold value.
  • Page 73 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.1.2 Microscope Control Panel Click the button to display barcode label area. To rotate the label area by 180°, click Figure 39 – Barcode label area window – Undo / Redo modification made during specifying the Scanmap. These buttons are inactive when Use threshold function is applied.
  • Page 74 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.1.2 Microscope Control Panel 5.1.2.3 Exposure Indicator Activate Exposure indicator function by clicking . Click to calculate exposure time for the actual Live image. In Brightfield mode there is no option for manually setting the values. Exposure time can be displayed in microsecond, millisecond, and also in second.

  • Page 75: Quick Preview Functions

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.1.3 Quick preview functions 5.1.3 Quick preview functions Use Ctrl+mouse wheel to zoom in or out to the preview area or the Live image. A quick preview function tool pops up by right-clicking to the preview area. It allows a quick access to...
  • Page 76 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.1.4 Quick microscope functions Autofocus • Live tools • Fast mode ◦ Screenshot ◦ Magnifier ◦ Ruler ◦ Image processor plugins ◦ Add scanmap ◦ Auto exposure • September 16, 2020 – Rev. 1 3DHISTECH Ltd.

  • Page 77: Fluorescence Microscope Mode

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.2 Fluorescence Microscope Mode 5.2 Fluorescence Microscope Mode Fluorescence Microscope View consist of three different sections: Profile Settings panel: Profile settings containing parameters used for scanning. • Microscope Control Panel: Live microscope movement functions •...
  • Page 78 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.2.1 Profile Settings panel E / Z (Multilayer option): Select Extended focus or Z-Stack scanning for the required channels • in accordance with the scanning mode set in the profile. D: Deconvolution can activated per channel in this column.
  • Page 79 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.2.1 Profile Settings panel If you deselect the Use fine focus checkbox, the software immediately starts scanning on the basis of the interpolated values, without pre-fine-focusing. Auto focus: Focus value is set automatically to match sample characteristics.
  • Page 80 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.2.1 Profile Settings panel Pre-scan + high-resolution – creates a High-Resolution fast FL preview image of every ◦ FOV within the selected area (in single layer DAPI, with an illumination time of 20ms) that is displayed in the Profile Wizard window.
  • Page 81 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.2.1 Profile Settings panel Everything within marker ◦ To scan everything within the marked area, check the Everything within marker selection box. Alternatively, click in the preview window to activate this function. Figure 47 – Scan everything within the marked area (selected / not selected) Threshold enhancement •...
  • Page 82 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.2.1 Profile Settings panel Caution! It requires lots of time (since it cannot find focus neither on the edges, nor on specks) to digitize coverslip edges. Select Remove coverslip function to eliminate those areas.
  • Page 83 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.2.1 Profile Settings panel Multilayer mode Single layer mode: If none of the filters are selected in the Filters table for multilayer • scanning, this mode is active and can be selected, and Multilayer mode turns inactive.
  • Page 84 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.2.1 Profile Settings panel Scan settings Change filters after whole slide scanning • To optimize speed, filter change should take place after the digitization of the slide with one channel. Change filters at each field-of-view •...
  • Page 85 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.2.1 Profile Settings panel Image Correction settings Use Flatfield correction – If this function is active the scanned slide compensates itself with a • special method (light intensity statistical data, not to modify the average intensity of the slide.
  • Page 86 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.2.1 Profile Settings panel Figure 50 – Image encoding window Compression • BMP – Lossless uncompressed image format, but larger files due to less effective ◦ compression. It is a raw data file.

  • Page 87: Microscope Control Panel

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.2.2 Microscope Control Panel 5.2.2 Microscope Control Panel 5.2.2.1 Preview window Click to open the Preview panel that offers options for threshold setting and selection of the area to be scanned. Figure 51 – Pre-scan preview image Threshold options This section is located at right side of the preview window.
  • Page 88 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.2.2 Microscope Control Panel Preview Types In FL Microscope mode the following additional preview modes are available: Brightfield preview – a normal type of illumination for scanning. This option is ◦ recommended when scanning in Brightfield mode.
  • Page 89 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.2.2 Microscope Control Panel Caution! It requires lots of time (since it cannot find focus neither on the edges, nor on specks) to digitize coverslip edges. Select Remove coverslip function to eliminate those areas.
  • Page 90 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.2.2 Microscope Control Panel Manual threshold • Set value manually or calculate value by using the Calculate Threshold function (can be activated only when manual thresholding is selected). This sets the sensitivity of the specimen detection.
  • Page 91 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.2.2 Microscope Control Panel Dilate mask Adjust the slider to enlarge or decrease the scanned area. To switch to manual mode click the top left corner of the button and the required value can be entered in the box Preview Toolbar This section is located at the bottom of the preview image.
  • Page 92 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.2.2 Microscope Control Panel Edit • The size of selected area (positive or negative) can be modified at the borderlines or corners. Figure 55 – Edited negative and positive selection masks Crop •...
  • Page 93 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.2.2 Microscope Control Panel TMA tool is used for selecting TMA spots on TMA slides. You can set the selection settings on the following four tabs: TMA Grid Setting • This function is enabled only if a TMA grid has already been placed on the preview image. Set grid parameters (row and column numbers) and spot shape (round or rectangle).
  • Page 94 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.2.2 Microscope Control Panel Clear scanmap • The entire scanmap area will be cleared and reset to default. Use selection box restriction • The preview image shows the area that will be digitized with a red and/or an orange mask. If the Use selection box is enabled, and the crop function is selected, a black frame displayed in the image, and only the area inside the box is scanned.
  • Page 95 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.2.2 Microscope Control Panel The following colors are used in the preview image to demonstrate digitization and selection settings: Figure 58 – Colors in preview image and Label area switching button Areas that will be scanned These areas are digitized on the basis of user settings (for example, Fill holes in scan area) and not detected by the selected threshold value.
  • Page 96 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.2.2 Microscope Control Panel Marker If Scan area inside the marker is selected in the Preview panel, the preview camera detects the marker pen. The inside areas appear in red color and will be digitized.
  • Page 97 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.2.2 Microscope Control Panel Click the button to display barcode label area. To rotate the label area by 180°, click Figure 60 – Barcode label area window – Undo / Redo modification made during specifying the Scanmap. These buttons are inactive when Use threshold function is applied.
  • Page 98 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.2.2 Microscope Control Panel For manual focusing define the manual focus step size by adjusting the Step size slider and use the two arrow buttons to focus manually. This is useful when you are interested in an area that remained blurry with Auto Focus (for example, the specimen is thicker in that area).
  • Page 99 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.2.2 Microscope Control Panel Image Intensity Click to open Image intensity panel. An image is defined as a two-dimensional function f(x, y) the amplitude of at any pair of coordinates (x, y) is called the intensity or gray level of the image at that point.

  • Page 100: Quick Preview Functions

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.2.3 Quick preview functions 5.2.3 Quick preview functions Use Ctrl + mouse wheel to zoom in or out to the preview area or the Live image. Figure 65 – Settings of preview quick toolbar A Quick Toolbar pops up by right clicking any of the control menu items.

  • Page 101: Quick Microscope Functions

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.2.4 Quick Microscope functions 5.2.4 Quick Microscope functions A quick microscope functions tool can be displayed upon right-clicking on the background of the main window (outside the preview window). It includes quick-access-buttons to main functions: Figure 66 –...
  • Page 102 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.2.4 Quick Microscope functions Microscope mode • Brightfield ◦ Fluorescence ◦ Figure 70 – Microscope mode Live tools • Fast mode ◦ Screenshot ◦ Magnifier ◦ Ruler ◦ Image processor plugins ◦ Use pseudo-color ◦...

  • Page 103: Bottom Menu Bar Of The Main Window

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.2.5 Bottom menu bar of the main window 5.2.5 Bottom menu bar of the main window Profile History This function can be activated by clicking in Preferences. During setting scanning profile all of the previously used parameter will be saved automatically.

  • Page 104: Planner Mode

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.3 Planner mode 5.3 Planner mode The whole scanning procedure can be planned in this mode. Assign profiles to slides, modify scan- maps without performing physical loading of slides. In Planner mode not only the scanning of the actually loaded magazine can be planned, but also profile assignment to slides of prepared virtual magazines is available to be performed.

  • Page 105: General Functions At The Main Menu

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.3.1 General functions at the main menu 5.3.1 General functions at the main menu Microscope/Planner selector Horizontal/Vertical selector (Available in Slide view with card mode) Stage Control – Available after initialization, upon clicking the slide icon or entering slide position number at the right side, the slide can be loaded.
  • Page 106 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.3.1 General functions at the main menu If you want to retake the preview of the actually loaded slide, the Retake Preview function is available from the Quick Toolbar of the preview window. Activate Retake Preview for all the slides in the magazine if necessary.

  • Page 107: General Functions At The Magazine Menu

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.3.1 General functions at the main menu Figure 77 – Profile management for all the magazines menu Jump to scanned – Loads the planner view of the actual slide during scanning Settings Help 5.3.2 General functions at the magazine menu...

  • Page 108: General Functions At The Slide Menu

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.3.3 General functions at the slide menu 5.3.3 General functions at the slide menu Mark empty slide positions – Define range in the Range to skip boxes to set multiple slide • positions as empty. For single slides, enter / set slide position with the arrows in the Range to skip box or right-click on a slide and select Skip.

  • Page 109: Slide View With Card

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.3.4 Slide view with card 5.3.4 Slide view with card Select from Preferences / Appearance settings / Planner layout selector to activate this view. Depending on the actual view preference selected at , slide positions can have a horizontal or vertical layout.
  • Page 110 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.3.4 Slide view with card Figure 81 – Horizontal slide view with card By clicking profiles can be added or removed for/from the slide, respectively. The parameters of the assigned profile is displayed in a card. The colors of a Brightfield and a Fluorescence profile cards are different.
  • Page 111 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.3.4 Slide view with card The following information are included in a profile card: Profile name in the top left corner • Profile status is marked with spots of different color: • – Stored ◦...
  • Page 112 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.3.5 Table view mode 5.3.5 Table view mode By clicking each slide will be displayed in a separate row. Click to assign profile to a slide, and to detach a profile from a slide. A slide can have more than one profile assigned to it, and these profiles can either be BF or FL if the scanner is capable to operate in these two scanning modes.

  • Page 113: Card Collection View Mode

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.3.6 Card collection view mode 5.3.6 Card collection view mode If you click the button, each of the slide previews are displayed in the window. Add profiles by clicking and detach profiles by clicking .

  • Page 114: Profile Editor For Scheduler

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.4 Profile editor for Scheduler 5.4 Profile editor for Scheduler Each of the assigned profiles can be verified and modified if necessary. The expanding panel at the right side includes all of the profile settings, in coherence with the view type of the Profile Setting panel.

  • Page 115: Progress Features

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.5 Progress features 5.5 Progress features The progress is visible both in Microscope and Planner modes whenever there is a running hardware operation or scanning procedure. Progress content can be therefore different depending on the type of the running procedure.

  • Page 116: Settings And Service

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.6 Settings and Service 5.6 Settings and Service Activate Settings by clicking and Services by clicking at the right side of the main menu bar. 5.6.1 Settings Activate Settings by clicking at the right side of the main menu bar.
  • Page 117 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.6.1 Settings Appearance settings View Mode Selector ◦ Text mode ▪ Icon mode ▪ Float Text mode ▪ Float Icon mode ▪ Style selector ◦ Black Jelly ▪ Flat style ▪ Black Jelly light ▪...
  • Page 118 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.6.1 Settings Default illumination mode selector – Brightfield or Fluorescence mode ▪ Simplified Scan Progress (for Brightfield scanning only) Screenshot folder – Set the folder for storing screenshots by clicking Browse. Otherwise the C:\Users\<Username>\Pictures folder is used by default.
  • Page 119 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.6.1 Settings Filter Wheel ▪ Flash Light ▪ General ▪ Halogen Light ▪ Microscope Part ▪ ▪ Objective Changer ▪ Preview Light ▪ RGB Light ▪ Relay ▪ Scan Loader ▪ Service ▪...
  • Page 120 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.6.1 Settings Slide name ▪ Slide index ▪ Rack index ▪ General naming settings Naming pattern – The set string defines the slide naming pattern (shown by the example below the text box). Click Reset to set pattern to default. The abbreviations and their meaning are listed at the bottom of the section under Usable abbreviations.
  • Page 121 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.6.1 Settings – Prescan settings • Figure 90 – Prescan settings Unlike Scan settings in Microscope or Planner mode, Prescan toolbar includes the same settings for pre-scanning but these depend on the hardware / filter settings.
  • Page 122 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.6.1 Settings Two of the three settings are responsible for opening external applications (.exe or .bat) as defined (for example, CaseViewer software can be opened after scanning is finished). These calls can run only the following scripts: ◦...
  • Page 123 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.6.1 Settings In the Message Call section of the window complex messaging functions can be defined. Here you can setup parameters of the mail server, add or remove users, and also set the message body in the Message subject field by entering a text for example, to inform recipients on the location of the scanner, or the slide set you are working with.
  • Page 124 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.6.1 Settings – Report sender settings • Figure 93 – Report sender settings Add new recipients to the list by clicking + or remove selected from the list by clicking -. To send an example mail to the selected recipient, click .

  • Page 125: Service

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.6.2 Service 5.6.2 Service Activate Service by clicking at the right side of the main menu bar. – Service password • Enter password first to enable service settings for modification. – Camera settings •...
  • Page 126 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.6.2 Service Magnification ◦ Use manual camera changer – Changing of the cameras is performed manually. ◦ Prescan objective protection enabled – If activated, slide area near the edges will not be ◦...
  • Page 127 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.6.2 Service Current threshold – Set the desired threshold level in the box. ◦ Use selection box – If selected, only the area inside the box is scanned. It is useful if ▪...
  • Page 128 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.6.2 Service Note: The maximum number of virtual filters is 50 if you insert penta filters (and this type is selected from the Filter type list) in the available 10 filter positions in the turret.
  • Page 129 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.6.2 Service – Image compensation • You can use a saved compensation image whenever compensation-related issues occur (deriving from the slide, illumination, staining or embedding medium irregularities). Figure 98 – Image compensation BF cameras field displays the active camera. Currently, there is only one active camera for ◦...
  • Page 130 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.6.2 Service Figure 99 – Barcode settings Note: If you did not set the barcode type correctly, or there is no barcode in the barcode area, the software will display the following error message: “Barcode detection unsuccessful! There might be no barcode present.”...
  • Page 131 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.6.2 Service Barcode reader library ◦ Select MIL barcode library or Embedded barcode library ▪ General Barcode settings ◦ Inverse barcode color ▪ Activate to invert foreground and background colors of the barcode.
  • Page 132 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.6.2 Service – Microscope control • Illumination ◦ Condensor covered ▪ Brightfield illumination ▪ Barcode area light ▪ Backlight illumination intensity ▪ Objective guide ◦ Step size ▪ X / Y values or navigation arrows ▪...

  • Page 133: Hosting

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.6.2 Service Click Start slide insert test button to launch the process. Slides are loaded in but preview image will not be recorded. If Magazine counter is set to -1, all the magazines will be tested in cycles based on the value set in the Loading cycle per magazine field.
  • Page 134 Pannoramic 250 FLASH III 3.0.2 User’s Guide 5.7 Hosting Hosting a teleconsultation session 1. Select Hosting from the microscope mode menu 2. Type in your nickname used during the session 3. Click to start hosting the session 4. The software displays the hosting address and the port number Join teleconsultation as a client 1.

  • Page 135: Using Profiles

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 6 Using Profiles 6 Using Profiles Note: Saved profile settings cannot be applied if using a different type of Pannoramic® scanner. If you want to scan a group of slides, it is advised to save your settings previously in a profile and continue scanning in Automatic mode.
  • Page 136 Pannoramic 250 FLASH III 3.0.2 User’s Guide 6 Using Profiles Use of removing specks according to the set value (ON/OFF) • Use saved scan area • Use selection box (ON/OFF) • Fluorescent settings Filter position(s) • Selected focus channel •...

  • Page 137: Troubleshooting And Maintenance

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 7 Troubleshooting and Maintenance 7 Troubleshooting and Maintenance 7.1 Recovery functions PSW automated backups allow you to continue scanning after unexpected events within as little time as possible. After the program is restarted and the microscope is initialized, you can confirm the automatic restore of pre-event state by clicking Yes.

  • Page 138: Cleaning The Device

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 7 Troubleshooting and Maintenance The product is not equipped with protection against hazardous materials (infectious, toxic, radioactive, corrosive substances or other effects damaging to health). Decontaminate infectious materials after use. Dust, specks and humidity may decrease or harm the operating capability of the device. Protect it with dust cover when not in use.

  • Page 139: Preventive Maintenance

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 7 Troubleshooting and Maintenance 7.2.3 Preventive maintenance To prevent fungal growth Do not leave the equipment in an unconditioned environment with a storage temperature • above 60°C (140°F) or below 0°C (32°F), which may damage the equipment.
  • Page 140 Pannoramic 250 FLASH III 3.0.2 User’s Guide 7 Troubleshooting and Maintenance 7. Switch on the power supply of the Pannoramic® 250 FLASH III (see chapter 1.1.4 Main parts of the Base Unit). 8. Switch on the control computer. 9. Launch control software.

  • Page 141: Fluorescent Applications (Optional)

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 7 Troubleshooting and Maintenance Digitization process is extremely slow Extended focusing option is activated • Deactivate the Extended focusing option if not required. • Z-stack option is activated • Deactivate the Z-stack option if not required.

  • Page 142: Maintenance

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 7 Troubleshooting and Maintenance 7.4 Maintenance This section describes the most common maintenance solutions that can be performed by the user of the product. For maintenance solutions not described in this manual, contact the service personnel.

  • Page 143: Replacing Fuses Of The Main Power Switch

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 7 Troubleshooting and Maintenance 7.4.2 Replacing fuses of the main power switch 1. Switch off the main power supply and disconnect the power cable (see chapter 1.1.4 Main parts of the Base Unit).

  • Page 144: Removing A Jammed Or Broken Slide

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 7 Troubleshooting and Maintenance 7.4.3 Removing a jammed or broken slide If a slide got jammed or has suffered breakage during the process of digitization, stop the process and, if possible, move the slide holder to a position where the slide can be easily removed by clicking the Emergency Manual Slide Removal Position button (see chapter 5.6.2 Service).
  • Page 145 Pannoramic 250 FLASH III 3.0.2 User’s Guide 7 Troubleshooting and Maintenance Figure 105 – Removing a slide 4. Press clamping lever (4) down. The slide position lock releases. 5. Retrieve slide (3) from the holder. 6. Release clamping lever (4).

  • Page 146: Installing And Removing The Filter Module (Fluorescent Option)

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 7 Troubleshooting and Maintenance 8. Press the slide loader (2) down to mechanical stop position, in which the locking lever must engage (1). Use moderate force to press the loader part opposite the motor in clamp direction, until this part mechanically engages, too.

  • Page 147: Removing A Magazine From The Transporting System

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 7 Troubleshooting and Maintenance 5. Hold the filter module (3) by its handling pads (one on the right, one on the left (lateral) side of the module) and insert it in forward tilted position from above into the upper spring- loaded clamps (1) at the filter wheel.
  • Page 148 Pannoramic 250 FLASH III 3.0.2 User’s Guide 7 Troubleshooting and Maintenance Warning! Crush hazard! Remember to stop the running digitization process, terminate the software session and turn power supply off before you remove a jammed magazine (see chapter 1.1.4 Main parts of the Base Unit).

  • Page 149: Transporting

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 7 Troubleshooting and Maintenance 7.4.6 Transporting The following procedure shows how to transport Pannoramic® 250 FLASH III. Figure 109 – Transporting handles Caution! Do not use the FL protective cover (2) to raise, shift or transport Pannoramic®...
  • Page 150 Pannoramic 250 FLASH III 3.0.2 User’s Guide 7 Troubleshooting and Maintenance Note: The base unit has four carrying handles for transporting. Two or four people can carry the base unit using these carrying handles. To determine the appropriate number of transporter people, observe the laws of your country regarding load carrying capacity limitations.

  • Page 151: Technical Data

    Pannoramic 250 FLASH III 3.0.2 User’s Guide 8 Technical Data 8 Technical Data Dimensions (width × depth × height) Base unit approx. 68×75×58 cm Base unit with fluorescence option approx. 68×95×58 cm Weight Base unit approx. 47 kg Base unit with fluorescent option approx.
  • Page 152 Pannoramic 250 FLASH III 3.0.2 User’s Guide 8 Technical Data Operating data Intended site closed room facilities Electrical protection class Internal protection degree IP 20 Electrical safety in compliance with DIN EN 61010-1:2001, DIN EN 61010-2- 101:2002 (IEC 61010-1:2001, IEC 61010-2-101:2002)
  • Page 153 Pannoramic 250 FLASH III 3.0.2 User’s Guide 8 Technical Data Scan cameras pco.edge 4.2 13.3 mm × 13.3 mm sCMOS (CIS2020A) Pixel size 6.5 µm × 6.5 µm Camera resolution 2048 × 2048 active pixel Frame rate 100 fps @ full resolution (2048 × 2048 pixel)
  • Page 154 Pannoramic 250 FLASH III 3.0.2 User’s Guide 8 Technical Data Camera resolution 4096 (H) × 3072 (W) pixel Max frame rate (with 40× objective) 180 fps Bit-depth 8 bit Pixel resolution with 20x objective and 1.6× C-mount 0.24µm adapter Pixel resolution with 40x objective and 1.6× C-mount 0.12µm...
  • Page 155 Pannoramic 250 FLASH III 3.0.2 User’s Guide 8 Technical Data Ports 1x CoaXPress Quad; 1x Double CameraLink port; 1x FireWire (1394 A); 4 × USB 2.0 Network connection 1 Gb/s Ethernet cable class CAT6A with Rj45 connector September 16, 2020 – Rev. 1 3DHISTECH Ltd.

  • Page 156: Index

    Lumencor SPECTRA 6..................20, 21, 24, 25, 26, 132 Marker............................129 Pannoramic............................7 Pannoramic 250 FLASH III..7, 8, 9, 10, 12, 14, 16, 18, 19, 20, 21, 22, 24, 25, 26, 30, 31, 133, 134, 136, 137, 139, 142, 143, 144 Pannoramic Viewer........................21, 134 Pco.edge 4.2......................13, 17, 20, 24, 153...

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