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3DHISTECH TMA Grand Master 2.1 User Manual

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TMA Grand Master
2.1
User's Guide
April 8, 2014
GM21-UG-EN Rev1
Rev. 1

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Summary of Contents for 3DHISTECH TMA Grand Master 2.1

  • Page 1 TMA Grand Master User’s Guide April 8, 2014 GM21-UG-EN Rev1 Rev. 1...

  • Page 2: Table Of Contents

    TMA Grand Master 2.1 – User’s Guide Contents Disclaimer............................4 Declaration Of Conformity......................6 Character Formats and Symbols....................7 Terms and abbreviations......................8 1 Intended Use..........................9 1.1 Main Features of TMA Grand Master....................9 2 TMA Grand Master Hardware....................10 2.1 TMA Grand Master Main Unit......................10 2.2 Cabling..............................11...
  • Page 3 TMA Grand Master 2.1 – User’s Guide 5.4 Inserting Donor Blocks.........................41 5.5 Launching the transfer process......................43 5.6 Slide overlay............................48 5.7 Exporting donor block data........................54 5.8 Import data............................63 5.9 Restore medical information database....................66 6 Maintenance of TMA Grand Master..................67 6.1 Preventive Maintenance of the Main Unit...................67 6.2 Cleaning Tasks............................67...

  • Page 4: Disclaimer

    IP are the sole properties of the 3DHISTECH Ltd. 3DHISTECH Ltd. is not liable for damage of whatever nature (including, but not limited to, general or specific damage, indirect damage, consequential damage or incidental damage, including the...
  • Page 5 Third-Party Disclaimer (LibTIFF – TIFF Library and Utilities) 3DHISTECH Ltd. is not liable for damage of whatever nature (including, but not limited to, general or specific damage, indirect damage, consequential damage or incidental damage, including damage resulting from lost profits, interruption of business, loss of business information, pecuniary loss or similar damage) that stems from or is associated with the incorporated part of this Product that is made by Silicon Graphics Inc.

  • Page 6: Declaration Of Conformity

    Declaration Of Conformity Declaration Of Conformity 3DHISTECH Ltd. declares that the product TMA Grand Master is designed and produced with consideration of specified requirements according to the IEC 61326-2-6:2005 standard, and conforms to the directives and other standards listed below: IEC 61010-1:2001 (ed.

  • Page 7: Character Formats And Symbols

    TMA Grand Master 2.1 – User’s Guide Character Formats and Symbols Character Formats and Symbols Example Abbreviation or term that is explained in the section Terms and Abbreviations. Example Words or characters that appear on the screen. These include field names, screen titles, pushbuttons and menu names, paths or options.

  • Page 8: Terms And Abbreviations

    TMA Grand Master 2.1 – User’s Guide Terms and abbreviations Terms and abbreviations Digital slide / Slide Slide digitized with a scanner microscope, for example Pannoramic SCAN. Consists of an MRXS file and a folder with the same name as the MRXS file.

  • Page 9: Intended Use

    TMA Grand Master 2.1 – User’s Guide 1 Intended Use 1 Intended Use TMA Grand Master is a software-controlled electronic laboratory device that automates the creation of tissue microarrays (TMAs) and also the process of PCR sampling. The creation of TMA blocks (by taking tissue core samples from donor blocks and inserting them into recipient blocks) and the insertion of extracted tissue cores into PCR tubes is controlled by using a graphical user interface.

  • Page 10: Tma Grand Master Hardware

    TMA Grand Master 2.1 – User’s Guide 2 TMA Grand Master Hardware 2 TMA Grand Master Hardware Hardware: TMA Grand Master main unit • Control computer with a monitor, a mouse and a keyboard • USB cables between the main unit and the control computer •...

  • Page 11: Cabling

    TMA Grand Master 2.1 – User’s Guide 2 TMA Grand Master Hardware 6. Grips for service compartment door 7. Front power supply switch Back View Figure 2 – TMA Grand Master back view 1. Terminal point for the power supply cord 2.

  • Page 12: Delivery

    TMA Grand Master 2.1 – User’s Guide 2 TMA Grand Master Hardware Important! The use of an uninterruptible power supply (UPS) is recommended but optional. Warning! Only connect external devices that are safety extra low voltage rated to the TMA Grand Master instrument to avoid the risk of electrical shock.

  • Page 13: Installing And Configuring Tma Grand Master Hardware

    Warning! Only qualified personnel may install TMA Grand Master and perform the initial start-up procedure. 3DHISTECH Service technicians install and configure TMA Grand Master and hand it over to you in operating condition. 2.5 Transporting the TMA Grand Master Main Unit...
  • Page 14 TMA Grand Master 2.1 – User’s Guide 2 TMA Grand Master Hardware 3. Lift the main unit by its recessed transporting handles that can be found both sides of the bottom plate, underneath the side-plates of the main unit. Figure 4 – Transporting handle 4.

  • Page 15: Installation Of Tma Grand Master Software Application

    500 GB – for saving the TMA database, and for storing digital slides 3.2 TMA Grand Master Software Installation Important! 3DHISTECH service technicians perform the installation and configuration of the TMA Grand Master software application. The following installation procedure is for information purposes only – follow the below steps only if you need to re-install the software, or upgrade to a newer software version.
  • Page 16 • Camera driver – VRmagic • 2. Launch the TMAGrandMaster_2.1_RTM.exe file that you received on a 3DHISTECH Installation Disk or downloaded in a compressed file. 3. Follow the instructions in the installation wizard. 4. Select the license type – browse for the license file or select HASP dongle.
  • Page 17 TMA Grand Master 2.1 – User’s Guide 3 Installation of TMA Grand Master Software Application 5. Accept the End-User License Agreement, and click Next. 6. Choose the installation folder for the TMA Grand Master software application. Click Browse for locating a different folder than the default one, then click Next to continue.
  • Page 18 TMA Grand Master 2.1 – User’s Guide 3 Installation of TMA Grand Master Software Application 7. Create the Start menu folder. 8. The installer automatically downloads the necessary prerequisite files. Click Install to start the installation of prerequisites. April 8, 2014 – Rev. 1 3DHISTECH Ltd.
  • Page 19 TMA Grand Master 2.1 – User’s Guide 3 Installation of TMA Grand Master Software Application 9. Click Install to continue with the installation of the TMA Grand Master application. 10. After completing the installation of TMA Grand Master, click Finish to close the wizard. The relating User's Guide can be opened if you activate the Show User's Guide option in this window.
  • Page 20 TMA Grand Master 2.1 – User’s Guide 3 Installation of TMA Grand Master Software Application NOTE: If Adobe Reader is already installed on your computer, the folder containing the user documentation will be opened automatically. Otherwise, a pop-up window is displayed offering you to download the application.

  • Page 21: Operating The Main Unit

    TMA Grand Master 2.1 – User’s Guide 4 Operating the Main Unit 4 Operating the Main Unit 4.1 Switching the Main Unit On or Off Turning the Power On 1. Switch on the TMA Grand Master (first, the main switch on the back panel – see Figure 2 (2), then the one in the front –...
  • Page 22 TMA Grand Master 2.1 – User’s Guide 4 Operating the Main Unit Warning! You receive a warning message after starting the software, if not having a valid license for running the application. A separate license is necessary to activate the PCR function of the TMA Grand Master software.
  • Page 23 TMA Grand Master 2.1 – User’s Guide 4 Operating the Main Unit To load an existing project, the following window is displayed after clicking Load, in which you can select the desired project to be loaded, and narrow project list by activating Date Range and/or Diameter filters.

  • Page 24: Main User Interface

    TMA Grand Master 2.1 – User’s Guide 4 Operating the Main Unit 4.3 Main User Interface This version of TMA Grand Master offers two different views of recipient and donor block panels. Based on the loading and processing method of blocks the normal view includes thumbnails of all recipient tray positions, and as for donor blocks the selected tray is displayed in its full capacity.

  • Page 25: Main Toolbar

    TMA Grand Master 2.1 – User’s Guide 4 Operating the Main Unit If you want to load a recipient block into the unit, click at the Unused Block(s) panel header. As for donor blocks, first click the button, then if there is a free slot on the actual tray, the door can be opened (otherwise wait until the closest tray with a free slot on it is rotated to loading position) and the block can be inserted.

  • Page 26: Inserting And Removing Blocks

    TMA Grand Master 2.1 – User’s Guide 4 Operating the Main Unit - Interrupt process - Door opening button - About TMA Grand Master (software version information) - Exit application 4.4 Inserting and Removing Blocks 4.4.1 Block preparation Warning! If the paraffin swells through the bottom or the sides of the plastic cassette, cut off the excess paraffin before inserting the block into the main unit.

  • Page 27: Cleaning Block And Pcr Cassette Preparation

    TMA Grand Master 2.1 – User’s Guide 4 Operating the Main Unit 4.4.2 Cleaning block and PCR cassette preparation Important! Before inserting a PCR cassette into its slot, ensure that the cover plate and tubes are uncontaminated. One PCR cassette can be inserted into its slot before the first position on each donor tray.

  • Page 28: Inserting A Block Into The Main Unit

    TMA Grand Master 2.1 – User’s Guide 4 Operating the Main Unit Important! Before starting a larger project it is advised to calculate the number of cores that will be inserted to PCR tubes, so placing a sufficient number of cleaning blocks accordingly.
  • Page 29 TMA Grand Master 2.1 – User’s Guide 4 Operating the Main Unit 1. Recipient block positions are displayed in a double-column on the left side of the screen. Tray positions of donor blocks are identified by six buttons (A-F), tray capacity is 10 blocks each.

  • Page 30: Removing A Block From The Main Unit

    TMA Grand Master 2.1 – User’s Guide 4 Operating the Main Unit 11. Follow the steps 1-9 if you want to insert additional blocks into other trays. If the cylinder is at the proper position, it is not necessary to the click the tray ID then the button.

  • Page 31: Changing The Drill Bit And The Puncher

    TMA Grand Master 2.1 – User’s Guide 4 Operating the Main Unit 4.5 Changing the Drill Bit and the Puncher TMA Grand Master is delivered with drill bits and punchers of different sizes. The following instructions demonstrate how to change drill bits, punchers and pistons to match the hole diameter of a defined layout.
  • Page 32 TMA Grand Master 2.1 – User’s Guide 4 Operating the Main Unit 4. Loosen the drill chuck locking lever (marked with red) that fixes the drill chuck in position and remove the drill chuck by lifting it upwards. Figure 8 – Removing the drill chuck 5.
  • Page 33 TMA Grand Master 2.1 – User’s Guide 4 Operating the Main Unit 9. Loosen the puncher chuck locking lever that keeps the puncher chuck in position and remove the puncher chuck assembled with the piston. Figure 10 – Removing the puncher chuck 10.

  • Page 34: Using Tma Grand Master

    TMA Grand Master 2.1 – User’s Guide 5 Using TMA Grand Master 5 Using TMA Grand Master This TMA Grand Master device is designed to create TMA blocks and make it available for you to place extracted cores into PCR tubes at the same time, within the same process. With the operating software you may set processes for creating TMA blocks or PCR sampling exclusively or both.

  • Page 35: Working With Recipient Block Layouts

    TMA Grand Master 2.1 – User’s Guide 5 Using TMA Grand Master Make sure that the cassettes with the paraffin blocks are properly inserted into the slots. • Make sure that the drill bit is of appropriate size and that it is fixed in its position.
  • Page 36 TMA Grand Master 2.1 – User’s Guide 5 Using TMA Grand Master 2. The Layout Editor window is displayed. 3. When you create a new pattern, you define the block layout. Type the name of your new layout into the Layout Name textbox (maximum length is 20 characters).
  • Page 37 TMA Grand Master 2.1 – User’s Guide 5 Using TMA Grand Master 9. If you want to create a pattern in which some of the grid positions (column/row intersection) are not marked as a hole to drill, disable those positions you want TMA Grand Master to skip during drilling.

  • Page 38: Modifying A Recipient Block Layout

    TMA Grand Master 2.1 – User’s Guide 5 Using TMA Grand Master 5.2.2 Modifying a recipient block layout 1. Open Layout Editor window by clicking the button next to the recipient block image. 2. To modify a block layout, select the name of the desired layout from the drop-down list, then click Edit.
  • Page 39 TMA Grand Master 2.1 – User’s Guide 5 Using TMA Grand Master In the new view for recipient blocks, the following options are available for you to choose from after the block thumbnail image is displayed in the Unused Block(s) panel: Click the block image then type in the ID, add •...
  • Page 40 TMA Grand Master 2.1 – User’s Guide 5 Using TMA Grand Master Warning! Make sure that: you have selected a correct layout for the inserted block, so that • corresponding dimension values, both of the layout and the block, are identical.

  • Page 41: Inserting Donor Blocks

    TMA Grand Master 2.1 – User’s Guide 5 Using TMA Grand Master 5.4 Inserting Donor Blocks 1. Insert donor blocks into the first tray, positions A1-10. For more information see section 4.4.3 Inserting a block into the main unit. Warning!
  • Page 42 TMA Grand Master 2.1 – User’s Guide 5 Using TMA Grand Master 4. If a slide or slides are found with matching IDs, their thumbnails are displayed in a window from which you can select one to use it as an overlay.

  • Page 43: Launching The Transfer Process

    TMA Grand Master 2.1 – User’s Guide 5 Using TMA Grand Master 6. If you want to remove a donor block from the device, click at the bottom right corner of the donor block thumbnail displayed at the donor panel.
  • Page 44 TMA Grand Master 2.1 – User’s Guide 5 Using TMA Grand Master When recipient (or cleaning) blocks, donor blocks, and PCR cassettes are inserted, the transfer process can be started. NOTE: If using TMA Grand Master in PCR mode only, and if necessary, it is available for you to insert cleaning blocks into empty slots during the process without interrupting it.
  • Page 45 TMA Grand Master 2.1 – User’s Guide 5 Using TMA Grand Master Important! PCR process is not supported in case the software was restarted after exiting without closing it properly or loading the project that has been saved previously. 2. If you want to remove an existing marker, click it with the right mouse button. The corresponding layout position of the deleted marker will therefore be free for the next defined marker.
  • Page 46 TMA Grand Master 2.1 – User’s Guide 5 Using TMA Grand Master 4. During the process, the colors of the cores on the layout varies according to their statuses. Color markings of cores and their statuses are as follows: – transferred;...
  • Page 47 TMA Grand Master 2.1 – User’s Guide 5 Using TMA Grand Master If you remove a donor block that is finalized (each selected core is transferred), and you are replacing it with a donor block having a similar text on its label area and label matching identifies it as the donor block you have removed, click the red cross icon next to the Donor ID field.

  • Page 48: Slide Overlay

    TMA Grand Master 2.1 – User’s Guide 5 Using TMA Grand Master If you replace the donor block but the PCR tube for the given position remains in the cassette and additional cores will be selected for PCR extraction, the PCR tube will contain cores from different blocks.
  • Page 49 TMA Grand Master 2.1 – User’s Guide 5 Using TMA Grand Master If locating a slide image on a CaseCenter server, type in the URL, then click CC connect. • Login to CaseCenter with your user name and password. April 8, 2014 – Rev. 1 3DHISTECH Ltd.
  • Page 50 TMA Grand Master 2.1 – User’s Guide 5 Using TMA Grand Master Browse for the slide in the folder tree, then left click on the slide to import it. • NOTE: Make sure that Pannoramic Viewer is the default program to open a slide from CaseCenter.
  • Page 51 TMA Grand Master 2.1 – User’s Guide 5 Using TMA Grand Master Position slide image (drag, rotate, zoom) to correspond with the block image. • Place two reference points on the block image, and refine their positions on the slide image.
  • Page 52 TMA Grand Master 2.1 – User’s Guide 5 Using TMA Grand Master You can fine-tune the position of the slide by moving the Fine Rotation slider to rotate the • image around the dark blue reference point. You can also modify the transparency level of the JPG image layer by moving the Opacity slider.
  • Page 53 TMA Grand Master 2.1 – User’s Guide 5 Using TMA Grand Master Markers become displayed with the same color as previously defined with the TMA Marker feature of the Pannoramic Viewer. NOTE: As marker colors and their names will be displayed in the relating cells of the...

  • Page 54: Exporting Donor Block Data

    TMA Grand Master 2.1 – User’s Guide 5 Using TMA Grand Master By setting the sufficient amount of transparency of the JPG image layer, marker positioning becomes easier. If you have finished, press the Start button to launch the transfer process.
  • Page 55 TMA Grand Master 2.1 – User’s Guide 5 Using TMA Grand Master 1. In mixed or TMA mode, after the TMA blocks are created and you want to save block-related data, click the Export button in the main toolbar and the Custom Export window appears.
  • Page 56 TMA Grand Master 2.1 – User’s Guide 5 Using TMA Grand Master Schemes can be defined based on database-driven project handling by creating a scheme or • selecting one from the Scheme drop-down list. When creating a new scheme, you can custom-define a record set from the SQL database of the related project.
  • Page 57 TMA Grand Master 2.1 – User’s Guide 5 Using TMA Grand Master 2. Moving blocks from the Recipient Blocks: field to the Coherent Blocks: field one-by-one by clicking creates separate databases for each block. Click the button to move all recipient blocks to the Coherent Blocks: field.
  • Page 58 TMA Grand Master 2.1 – User’s Guide 5 Using TMA Grand Master To create a single database summing up data for multiple blocks, you can group them by left clicking while holding the Ctrl key on the Group items, and finally click the...
  • Page 59 TMA Grand Master 2.1 – User’s Guide 5 Using TMA Grand Master 5. If the software finds a Donor ID that already exists in the project, the relevant datasets will be merged together. In order to search for an ID in a specific column, the column itself can be selected manually.
  • Page 60 TMA Grand Master 2.1 – User’s Guide 5 Using TMA Grand Master The generated spreadsheet contains three worksheets: the first one is the Lookup on which there are six columns. Unique TMA Sample ID, which is the position of the tissue core in the recipient block, the TMA Block ID if you are using several blocks in the same project, and the Donor Block ID.
  • Page 61 TMA Grand Master 2.1 – User’s Guide 5 Using TMA Grand Master The second sheet contains recipient block-related data table where the core IDs are shown according to their positions in the layout. The third sheet contains recipient block-related data table where the Donor Block IDs are shown according to their positions in the layout.
  • Page 62 TMA Grand Master 2.1 – User’s Guide 5 Using TMA Grand Master In mixed mode, the application creates a separate file for PCR-related data. The file name is extended with the PCR suffix. The worksheet includes Donor ID and image with the relevant number of PCR sample.

  • Page 63: Import Data

    TMA Grand Master 2.1 – User’s Guide 5 Using TMA Grand Master 5.8 Import data To import donor block-related data, do the following: 1. Click Import at the main menu bar of the application window. 2. Click Browse in the Import window to search for the data file to be imported. If the ID of the donor block loaded into the device exists in the imported database, the data set of the specific donor block can be appended to the database.
  • Page 64 TMA Grand Master 2.1 – User’s Guide 5 Using TMA Grand Master 3. The content of the selected data sheet can be modified with the Merge or the Replace function. The imported data are stored in the MedicalInfos data sheet that can be selected as a record when exporting.
  • Page 65 TMA Grand Master 2.1 – User’s Guide 5 Using TMA Grand Master In this case, the already existing data is archived and can be accessed after clicking Settings. Click Restore in the Settings window to restore the original data set (see section 5.8 for more information).

  • Page 66: Restore Medical Information Database

    TMA Grand Master 2.1 – User’s Guide 5 Using TMA Grand Master 5.9 Restore medical information database To restore medical information database, therefore omitting all the data during handling the project before exporting, click Settings at the main toolbar. April 8, 2014 – Rev. 1 3DHISTECH Ltd.

  • Page 67: Maintenance Of Tma Grand Master

    TMA Grand Master 2.1 – User’s Guide 6 Maintenance of TMA Grand Master 6 Maintenance of TMA Grand Master Follow the maintenance and cleaning tasks discussed in this section to ensure the fail-safe operation of the TMA Grand Master device.

  • Page 68: Opening The Service Compartment Door

    TMA Grand Master 2.1 – User’s Guide 6 Maintenance of TMA Grand Master 6.2.1 Opening the Service Compartment Door Service compartment might be cleaned in order to remove dust or objects dropped in. To remove the door of the service compartment do the following: 1.

  • Page 69: Replacing Fuses Of The Main Power Switch

    TMA Grand Master 2.1 – User’s Guide 6 Maintenance of TMA Grand Master 6.3 Replacing fuses of the main power switch 1. Switch off the main power supply and disconnect the power cable. 2. The fuse compartment is located next to the main power supply switch at the back side of the TMA Grand Master.

  • Page 70: Contaminated Tray Or Touch Sensor Surface

    TMA Grand Master 2.1 – User’s Guide 6 Maintenance of TMA Grand Master 5. Replace fuses, put the insert back into its mounting, and push it inwards until you hear a snap, so the lever locks. Figure 17 – Replacing the fuses Important! Use T3.15A/250V fuses only.
  • Page 71 TMA Grand Master 2.1 – User’s Guide 6 Maintenance of TMA Grand Master According to the instructions in the following message, by using a dry and clean cloth, gently • rub the bottom surface of the touch sensor (sensor is marked with red in Figure 18).

  • Page 72: Troubleshooting Tma Grand Master

    TMA Grand Master 2.1 – User’s Guide 7 Troubleshooting TMA Grand Master 7 Troubleshooting TMA Grand Master 7.1 Hardware-related issues TMA Grand Master Does Not Work at All What to do: Contact 3DHISTECH support. TMA Grand Master software stops working (due to unexpected error) What to do: Exit application, then start again.

  • Page 73: Software-Related Issues

    The System Administrator must ensure accessibility by the control software to some folders on the computer. For instance, write access must be granted to C:\ProgramData\3DHISTECH\TMAGrandMaster folder, its sub-folders/files, and in addition, enable the software to modify registry logs. Block image and label area not visible Cause: Problem with preview camera –...

  • Page 74: Cassette And Block Dimensions

    24 mm (H) x 37 mm (W) x 7.5 mm (D) • NOTE: If using the 3DHISTECH block moulder, the thickness (depth) of the block is 13.5 mm. The smaller size (7.5 mm in depth) means the paraffin thickness from the upper edge of the •...

  • Page 75: Technical Description Of The Hardware

    TMA Grand Master 2.1 – User’s Guide 9 Technical Description of the Hardware 9 Technical Description of the Hardware 9.1 Physical Dimensions and Weight Name of the Unit Dimensions in mm* Weight in kg* (Width x Depth x Height) TMA Grand Master main unit 807×505×444...

  • Page 76: Objective And Cameras

    TMA Grand Master 2.1 – User’s Guide 9 Technical Description of the Hardware 9.3 Objective and Cameras Component Description Objective 16 mm focal length Camera type VR Magic Camera resolution 3,14 Million Pixel 9.4 Ambient Conditions Condition Suitable Range Shipment (packed) Ambient temperature -40°C to +70°C...

  • Page 77: Operating Data

    TMA Grand Master 2.1 – User’s Guide 9 Technical Description of the Hardware 9.5 Operating Data Category of operating environment closed room facilities Electrical protection class Internal protection degree IP 20 Electrical safety in compliance with EN 61010-1 (IEC 61010-1) and taking CSA &...

  • Page 78: Related Documents

    TMA Grand Master 2.1 – User’s Guide Related documents Related documents Pannoramic Viewer 1.15.3 User’s Guide TMA Module 1.16 User’s Guide April 8, 2014 – Rev. 1 3DHISTECH Ltd. 78(79)

  • Page 79: Index

    TMA Grand Master 2.1 – User’s Guide Index Index Barcode............................42 Block............................43, 48 Donor block......8, 9, 13, 29, 30, 34, 41, 42, 43, 44, 45, 46, 47, 48, 52, 54, 60, 61, 70, 72 Drill...................9, 12, 29, 31, 32, 34, 35, 36, 37, 67, 74, 75 Excel............................13, 59...

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