Heraeus HS 9 Service Manual page 192

Microbiology safety cabinets
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Testing Safety Cabinet Class II according to EN 12 469
HS / HSP
Visual check
• Labels, warnings
• Scratches
• Exhaust connection
• Options
Electrical safety test ( VDE )
Ground conductor
( Schutzleiter )
Insulation resistance ( Isolationswiderstand )
Leakage current
( Ableitstrom )
Air direction test
• Adjust front window to working position ( 200 mm )
• Slide along the window opening with smoke tube
• Air flow direction from outside to inside
( no refluxing, no visible turbulence )
Inflow air velocity test
Inflow air speed (method 1)
Adjust front window to an opening of 5 cm
Measuring air velocity in the middle in steps of
10 cm (start with 5 cm)
Inflow air can be calculated: v
Exhaust air speed (method 2)
Place the measuring frame over the exhaust
filter opening and seal with adhesive tape
Measure air velocity according to 5-position
method
Inflow air can be calculated: v
Laminar air flow test
Cabinet in operation ( at least 20 min.)
Adjust anemometer 5 cm above front window
opening
Measuring grid: of the edge 15 cm,
between measure point 30 cm
Measure air velocity (wait 1 min.)
Mean value of air velocity: 0,25 - 0,5 m/s + 20 %
If air velocity is too low, test the following:
• Voltage of the fans
• Exhaust connection
• Ducting system
Filter leakage test (DEHS- test)
Main filter and exhaust filter
• Turn cabinet on
• Calibrate particle counter
• Place aerosol generator in the cabinet
• Connect rarefaction between particle counter and
test connection (under working plate)
Testing HS / HSP
• Only HSP: Remove rubber seal from bypass opening
• Switch on aerosol generator
• Measure particles at the dirty side of main or exhaust
• Required value: 30 mil. particles of size 0.3 µm
• Disconnect the particle counter from the rarefaction
• Scan the seat of the sealing an the clean side of the
• Limit value of particles > 300 St./ 0.3 µm
• If max. number of particles is exceeded change filter.
Monitoring equipment, acoustic and visual alarm
• Cabinet in operation
• Measure fan voltage
• Perform test for laminar air ( mean value 0,40 m/s )
__
= v
/ 4
2
2
= v
x A
/A
2
1
1
2
• Return fan voltage to the measured value
• Turn cabinet off and on, check operation
Fill out test protocol and paste up inspection sticker
Additional: KI-Discus test (personnel protection test)
• Cabinet in operation (at least 20 min)
• Reduce air movements in laboratory ( close doors,
• Install KI-Discus equipment according to its operating
• Do measurement 5 times according to instruction
• Count potassium iodide particles on filter paper
• Calculate protection factor
• Limit < 60 particles
• For deviations: repeat measurement 2 times
• Check air flow in laboratory, check filter sealing
Ingo Nierobisch
filter, 5 measurements (each for 1 min)
and connect to the measuring-head
main filter. Speed of scanning < 5 cm/sec, distance
max. 1 cm.
Adjust lower limit pressure switch
Find out point with lowest air velocity
Set anemometer in this point, measure air
velocity
Set fan voltage so that at this measuring point air
velocity is mean value -20 % ( 0,32 m/s )
Change adjustment of lower pressure switch it
just turns on. Visual and acoustic alarm goes on
Adjust upper limit pressure switch
Find out point with highest air velocity
Set anemometer in this point, measure air
velocity
Set fan voltage so that at this measuring point air
velocity is mean value +20 % ( 0,48 m/s )
Change adjustment of upper pressure switch it
just turns on. Visual and acoustic alarm goes on
windows, air condition, ... )
instruction
14.06.02

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