Thermo Scientific TCF-32 Instruction Manual page 43

8 Unloading (In case of density gradient centrifugation)
1 After completion of sedimentation, press
STOP and decelerate the rotor down to 3,000
rpm.
(In the case of ZONAL mode operation, the
rotor is automatically decelerated down to
3,000 rpm and stabilized.)
With the rotor kept spinning at 3,000 rpm,
detach the tube from the outer connector and
connect an air-block tube instead.
The air-block tube is necessary to introduce
the extrusion solution to the external wall of
the rotor.
At this time, fill the tube to be injected with
extrusion solution. Use an extrusion solution
the density of which is higher than the density
gradient solution or cushion solution injected
into the rotor.
2 Send extrusion solution from the outer
connector, and push out the separated sample
and density gradient solution from the central
connector. Thereafter, stop the rotor.
Unloading (In case of pelleting)
After completion of sedimentation, decelerate the rotor to a stoppage. Disassemble
the rotor, and recover pellets.
-24-
Tube for air block
(60~70 cm)
Air inside
Fill extrusion
solution
Extrusion solution