Usage; General Description On Usage - Thermo Scientific TCF-32 Instruction Manual

4. USAGE

4.1 General description on usage

The continuous flow rotor is employed in the following manner :
Procedure Injection of density
gradient solution, buffer
Item solution
Solution flow
Spinning speed
Inject density gradient
Density gradient
solutions from lighter
centrifugation
ones
Pelleting Inject buffer solution
CAUTION : This rotor should never be spinned at a speed over 3,000 rpm unless all the parts such
as rotor assembly, seal attachment assembly and lubricating unit assembly are mounted in
place and also with the rotor not filled with the liquid.
If these cautions are not observed, shaft (D) could be broken or the core could be damaged.
Table 3 Separation procedure
3,000 rpm
Continuously inject
sample at specified flow
rate
Centrifugal separation
Specified speed
-11-
Recovery
3,000 rpm
Inject high density
extrusion solution, and
recover separated
solution
Stop and disassemble
rotor, and recover