Capacity In Rotor - Thermo Scientific TCF-32 Instruction Manual

4.2.4 Stoppage
1
Turn off the OIL, WATER and DRAIN switches on the lubricating unit. And turn off the
switch on the low temperature circulating water cooling bath. At this status, cooling water
remains in the seal attachment assembly. Drain the cooling water by connecting the cooling
water supply pipe to the DRAIN connector.
2
Disassemble and remove the seal attachment in the procedure reverse to assembly. Clamp
by a pinch cock or others the OIL supply pipe on the bearing housing to prevent oil from
flowing out of the bearing housing.
3
Remove the door adapter, open the door, and take out the rotor gently.
4
Remove the shaft (D) by loosening the swagelok nut.
Install the rotor on the Rotor base, and remove the cover.
Pull out the core by means of the core handle.
Here, recover pellets, if any.
5
As for the centrifuge, clean the chamber interior, close the door, mount the door hole cover,
and turn off the power switch. Although a small amount of oil leaks into the chamber, this is
normal.

4.3 Capacity in rotor

Fig. 13 and 14 show the relationship between the radius and capacity in the rotor. Use them as
references for determining the injected volumes of density gradient solution and cushion solution.
The capacity of separation space in rotor is approx. 430 ml . However, since there is a capacity
of approx. 25 ml in the flow path of seal body assembly and center of rotor, the total capacity is
approx. 455 ml as shown in fig. 14.
R3
R2
R1
Fig.13 Radius in
rotor
R (mm)
Fig.14 Capacity
-25-