Wavelength Calibration - Agilent Technologies 1200 Infinity Series User Manual

8
Test Functions and Calibration

Wavelength Calibration

Wavelength Calibration

The detector uses the alpha (656.1 nm) and beta (486 nm) emission lines of
the deuterium lamp for wavelength calibration. The sharp emission lines
enable more accurate calibration than is possible with holmium oxide. When
recalibration is started, the 1 nm slit is moved into the light path automatically
(G4212A). The gain is set to zero.
On completion of the scan, the alpha- and beta-line deviations (in nm) are
displayed. These values indicate how far the detector calibration deviates from
the actual positions of the alpha and beta emission lines. After calibration, the
deviation is zero.
To eliminate effects due to absorbing solvents, install the Max-Light Cartridge
Test Cell before starting the test.
Improved wavelength calibration algorithm
The wavelength calibration algorithm has been changed to higher accuracy in
UV wavelength range for G4212A/B DAD and K4212B DAD Clincal ed. with
firmware B.06.33.
It has been found, that after a wavelength re-calibration (using the Agilent
LabAdvisor or Instant Pilot), the measured wavelength in the lower UV might
be out of the specified range of +1/-1 nm.
Example: Using Caffeine and measuring at 205 nm.
The higher wavelength range is not effected.
When The detector is calibrated at the factory, and under normal operating conditions should not require
recalibration. However, it is advisable to recalibrate:
• after maintenance (flow cell or UV-lamp),
• after repair of components in the optical unit,
• after exchange of the optical unit or main board,
• after significant environmental condition changes (temperature, humidity),
• at a regular interval, at least once per year (for example, prior to an Operational
• when chromatographic results indicate the detector may require recalibration.
Parts required #
1
1
Preparations • Detector/lamp must be on for more than 1 hour.
• When using a Max-Light Cartridge Cell a flow rate of 0.5 mL/min with water is required.
156
Qualification/Performance Verification procedure), and
Description
Max-Light Cartridge Test Cell or
Max-Light Cartridge Cell
Agilent 1200 Infinity Series DAD User Manual