Use Of Max-Light Cartridge Test Cell - Agilent Technologies 1200 Infinity Series User Manual

Use of Max-Light Cartridge Test Cell

The Max-Light Cartridge Test Cell is recommended to be used for several tests
instead of the Max-Light Cartridge Cell (10 mm, V( σ) = 1 μL) or the Max-Light
Cartridge Cell (60 mm, V( σ) = 4 μL) because it allows running the test(s)
without any influence of the rest of the system (degasser, pump, sampler and
others).
The results of the test cell are comparable with the Max-Light Cartridge Cell
(10 mm, V( σ) = 1 μL) filled with water, e.g. Intensity Profile. Only the
Absorbance value is higher on the Max-Light Cartridge Cell.
If the profile of the Max-Light Cartridge Cell differs in the low UV range, then
absorbing solvents are in the cell and should be flushed out. See also
of Max-Light Cartridge Cell"
When using the Max-Light Cartridge Cell for tests/calibrations, it should be run at
N O T E
0.5 mL/min constant flow with water. This assures that the light path is always flushed.
Below table gives an idea on the signal height variation of the Max-Light
Cartridge Cells compared to Max-Light Cartridge Test Cell.
Table 11
Part Number
G4212-60011
G4212-60008
G4212-60007
G5615-60018
G5615-60017
G4212-60032
G4212-60017
Agilent 1200 Infinity Series DAD User Manual
on page 178.
Max-Light Cartridge Cells compared to Max-Light Cartridge Test Cell
Description
Max-Light Cartridge Test Cell
Max-Light Cartridge Cell 10 mmV(
Max-Light Cartridge Cell 60 mmV(
Max-Light Cartridge Cell Bio-inert 10 mmV(
Max-Light Cartridge Cell Bio-inert 60 mmV(
Max-Light Cartridge Cell HDR (3.7 mm, V(
Max-Light Cartridge Cell ULD (10 mm, V(
Test Functions and Calibration

Use of Max-Light Cartridge Test Cell

Signal Height (typical)
100 %
σ
~ 100 %
) = 1 µL
σ
~ 100 %
) = 4 µL
σ
~ 100 %
) = 1 µL
σ
~ 100 %
) = 4 µL
σ
100 %
) 0.4 µL)
σ
100 %
) 0.6 µL)
8
"Cleaning
135