Agilent Technologies 1200 Infinity Series User Manual
  1. Manuals
  2. Brands
  3. Agilent Technologies Manuals
  4. Laboratory Equipment
  5. 1200 Infinity Series
  6. User manual
Agilent Technologies 1200 Infinity Series User Manual

Agilent Technologies 1200 Infinity Series User Manual

High dynamic range diode array detector solution
Hide thumbs Also See for 1200 Infinity Series:
Table of Contents

Advertisement

Quick Links

Download this manual
Agilent 1200 Infinity Series
High Dynamic Range Diode
Array Detector Solution
User Guide
Agilent Technologies

Advertisement

Table of Contents
loading

Related Manuals for Agilent Technologies 1200 Infinity Series

Summary of Contents for Agilent Technologies 1200 Infinity Series

  • Page 1 Agilent 1200 Infinity Series High Dynamic Range Diode Array Detector Solution User Guide Agilent Technologies...
  • Page 2 Notices Warranty © Agilent Technologies, Inc. 2014-2016, receive no greater than Restricted Rights as 2017 defined in FAR 52.227-19(c)(1-2) (June The material contained in this docu- 1987). U.S. Government users will receive No part of this manual may be reproduced ment is provided “as is,”...
  • Page 3 In This Book In This Book This guide covers the Agilent 1200 Infinity Series High Dynamic Range Diode Array Detector Solution (HDR-DAD) Find detailed information on stack configurations and leak and waste handling in the manuals of the modules, that are necessary to run this solution.

  • Page 4: Table Of Contents

    30x wider linear UV-range – Quantification of widely different concentrations in a single run HDR-DAD Solution Data-Representation 2 Specifications Specifications of the 1200 Infinity Series High Dynamic Range Diode Array Detector Solution 3 Installation of the HDR-DAD Solution Unpacking the Solution Kit...

  • Page 5: What Is The Hdr-Dad Solution

    HDR-DAD Solution What is the HDR-DAD Solution? 30x wider linear UV-range – Quantification of widely different concentrations in a single run HDR-DAD Solution Data-Representation This chapter provides an overview of HDR-DAD and its intended use. Agilent Technologies...

  • Page 6: 30X Wider Linear Uv-Range - Quantification Of Widely Different Concentrations In A Single Run

    What is the HDR-DAD Solution? 30x wider linear UV-range – Quantification of widely different concentrations in a single run 30x wider linear UV-range – Quantification of widely different concentrations in a single run The typical linear range of around 1·10 for UV-detection is not sufficient for the analysis of samples or compound mixtures with widely different concentration levels.
  • Page 7 What is the HDR-DAD Solution? 30x wider linear UV-range – Quantification of widely different concentrations in a single run This is achieved by Agilent's Max-Light flow cells. Optofluidic waveguides in these flow cells facilitate total internal reflection for superior light transmission.

  • Page 8: Hdr-Dad Solution Data-Representation

    What is the HDR-DAD Solution? HDR-DAD Solution Data-Representation HDR-DAD Solution Data-Representation The HDR-DAD solution combines two Agilent Diode Array Detectors (possible combinations: G4212A/B x G4212A/B or G7117A/B x G7117A/B). The two detectors transfer their signals to the software. The algorithm of the HDR-DAD solution combines and presents all signals to the user.
  • Page 9 What is the HDR-DAD Solution? HDR-DAD Solution Data-Representation The HDR-DAD solution is available in ChemStation and EZChrom. Both software solutions N O T E provide the same results. Nevertheless, the two software solutions show different starting points of the HDR-DAD signal: •...
  • Page 10 What is the HDR-DAD Solution? HDR-DAD Solution Data-Representation HDR-DAD Solution...

  • Page 11: Specifications

    HDR-DAD Solution Specifications Specifications of the 1200 Infinity Series High Dynamic Range Diode Array Detector Solution This chapter provides information on performance specifications. Agilent Technologies...

  • Page 12: Specifications Of The 1200 Infinity Series High Dynamic Range Diode Array Detector Solution

    Specifications Specifications of the 1200 Infinity Series High Dynamic Range Diode Array Detector Solution Specifications of the 1200 Infinity Series High Dynamic Range Diode Array Detector Solution Table 2 Performance Specifications of the HDR-DAD solution Type Specification Comments Detection type...
  • Page 13 Specifications Specifications of the 1200 Infinity Series High Dynamic Range Diode Array Detector Solution Table 2 Performance Specifications of the HDR-DAD solution Type Specification Comments Analog outputs Recorder/integrator: 100 mV or 1 V, output range 0.001 – 2 AU, one output...
  • Page 14 Specifications Specifications of the 1200 Infinity Series High Dynamic Range Diode Array Detector Solution HDR-DAD Solution...

  • Page 15: Installation Of The Hdr-Dad Solution

    HDR-DAD Solution Installation of the HDR-DAD Solution Unpacking the Solution Kit Flow connections and network setup Enabling the HDR-DAD solution Configuring the HDR-DAD Solution Calculation of the Delay Volume This chapter provides information on installation of the HDR-DAD Solution. Agilent Technologies...

  • Page 16: Unpacking The Solution Kit

    Installation of the HDR-DAD Solution Unpacking the Solution Kit Unpacking the Solution Kit Ensure all parts and materials have been delivered. The delivery checklist for the 1200 Infinity HDR-DAD Solution Kit (G2199AA) is shown below. Description 5067-4660 Capillary, SST 0.11 mm ID 220 mm long 5063-6524 Caffeine in Water, 10 mg/L, 50 mL 5022-2159...

  • Page 17: Flow Connections And Network Setup

    Flow connections and network setup For details on the installation of the entire stack, the detector and the flow cell, see the manual Agilent 1200 Infinity Series Diode Array Detectors or Agilent 1290 Infinity II Diode Array Detector. Parts required...
  • Page 18 Installation of the HDR-DAD Solution Flow connections and network setup 1 Install the DAD on top of DAD Solvent cabinet DAD 2 Inlet DAD 2 Capillary Outlet DAD 1 DAD 1 Pump Figure 3 Stack and capillary connection for HDR-DAD (1290 Infinity Stack as an example) 2 Connect the capillaries from the TCC to DAD and the outlet of DAD...
  • Page 19 Installation of the HDR-DAD Solution Flow connections and network setup 3 Setup the network, for an example see “Local LAN connection to a PC (not via corporate LAN)” on page 20. • Connect both detectors to the LAN and in addition connect all the devices via CAN bus. N O T E •...
  • Page 20 Installation of the HDR-DAD Solution Local LAN connection to a PC (not via corporate LAN) Local LAN connection to a PC (not via corporate LAN) LAN-cable DAD SHT (short) DAD LNG (long) LAN-cable Network switch HDR-DAD Solution...

  • Page 21: Enabling The Hdr-Dad Solution

    Installation of the HDR-DAD Solution Enabling the HDR-DAD solution Enabling the HDR-DAD solution Parts required Description HDR-DAD USB dongle Contains licence to enable HDR-DAD solution 5188-8049 USB A F-USB Mini B M OTG (Module to Flash Drive) For the installation of the HDR-DAD USB dongle into a G7117A/B Only one of the DADs needs a license (DAD or DAD N O T E...
  • Page 22 Installation of the HDR-DAD Solution Enabling the HDR-DAD solution Figure 4 Rear view (G4212A/B) Figure 5 Rear view (G7117A/B) For the installation of the HDR-DAD USB dongle into a G7117A/B the USB A F-USB N O T E Mini B M OTG (Module to Flash Drive) (5188-8049) (part of the HDR-DAD Solution Kit) is required.

  • Page 23: Configuring The Hdr-Dad Solution

    Installation of the HDR-DAD Solution Configuring the HDR-DAD Solution Configuring the HDR-DAD Solution The instrument can be configured via the OpenLAB Control Panel, when setting up the device there. Software required • OpenLAB CDS A.01.05 (or higher) • ChemStation Edition C.01.05 (or higher), or •...
  • Page 24 Installation of the HDR-DAD Solution Configuring the HDR-DAD Solution 3 Click Yes. 4 Enter the IP address or Hostname of the DAD with the long cell and click OK. After Auto-Configuration the Post Auto Configuration dialog is shown. HDR-DAD Solution...
  • Page 25 Installation of the HDR-DAD Solution Configuring the HDR-DAD Solution 5 To configure the instrument click Configure HDR-DAD Cluster. In case the prerequisites are not fulfilled, the button is not active. A tooltip informs about N O T E the reason. The detector with the longest cell is always the first detector in the Detectors list.
  • Page 26 Installation of the HDR-DAD Solution Configuring the HDR-DAD Solution 6 Define the Delay Volume between detectors (calculate the delay volume upfront, see “Calculation of the Delay Volume” on page 30). Delay volume The default value as specified in the software is 11.0 µL. N O T E Recheck for correct delay compensation in additional runs.
  • Page 27 Installation of the HDR-DAD Solution Configuring the HDR-DAD Solution 7 Specify the linked pump. Linked Pump The Linked Pump is used to recalculate the delay for different flow values. N O T E 8 Configure the second LAN connection. a Select the detector with the shorter cell and click Configure. Detector with short cell HDR-DAD Solution...
  • Page 28 Installation of the HDR-DAD Solution Configuring the HDR-DAD Solution b Click Additional connection... c Check Use auxiliary connection, enter IP Address or Hostname of the detector with the shorter cell and click OK. HDR-DAD Solution...
  • Page 29 Installation of the HDR-DAD Solution Configuring the HDR-DAD Solution 9 In HDR-DAD Cluster Configuration (auto configuration part) click OK. The HDR-DAD solution now is configured. Figure 7 Configured system No other detectors are supported together with HDR. N O T E HDR-DAD Solution...

  • Page 30: Calculation Of The Delay Volume

    Installation of the HDR-DAD Solution Calculation of the Delay Volume Calculation of the Delay Volume Parts required Description 5022-2159 Restriction capillary, SST 0.12 mm ID, 2 m long 5063-6524 Caffeine in Water, 10 mg/L, 50 mL IQ test sample, for UV/VIS spectrophotometer No shipment of caffeine standard to China.
  • Page 31 Installation of the HDR-DAD Solution Calculation of the Delay Volume 3 Calculate the Delay Volume. Figure 8 Calculation of the Delay Volume Delay Time: T = RT - RT delay DAD 2 DAD 1 Delay Volume: V = Flow rate * T delay delay 4 Open the High Dynamic Range DAD Configuration, enter the calculated delay...
  • Page 32 Installation of the HDR-DAD Solution Calculation of the Delay Volume HDR-DAD Solution...

  • Page 33: Using The Hdr-Dad Solution

    Method and Run Control User Interface in ChemStation HDR-DAD Solution - Data Analysis This chapter provides information on the main function of the user interface for the HDR-DAD solution. For more details refer to the software manual or the online help. Agilent Technologies...

  • Page 34: Status Information And Module Information Dialog Of The Hdr-Dad Cluster

    Using the HDR-DAD Solution Status Information and Module Information Dialog of the HDR-DAD Cluster Status Information and Module Information Dialog of the HDR-DAD Cluster For details on how to use the controller software, see the manual Agilent 1200 Infinity N O T E Series Diode Array Detectors, Agilent 1290 Infinity II Diode Array Detector or the online help.
  • Page 35 Using the HDR-DAD Solution Status Information and Module Information Dialog of the HDR-DAD Cluster • Tooltips show lamp (see Figure 11 on page 35) and cell (see Figure 12 page 35) tag information for DAD and DAD Figure 11 Lamp tag information Figure 12 Cell tag information HDR-DAD Solution...
  • Page 36 Using the HDR-DAD Solution Status Information and Module Information Dialog of the HDR-DAD Cluster • The module information dialog shows information about the access point and the additional 2 LAN connection of the HDR-DAD cluster. Figure 13 Information on Additional Connection and Access Point HDR-DAD Solution...

  • Page 37: Method Settings In The Hdr-Dad Solution

    For details on method settings as they are important for general use of the DAD, see the manual Agilent 1200 Infinity Series Diode Array Detectors, Agilent 1290 Infinity II Diode Array Detector or the online help. The following method setting are relevant for the HDR-DAD solution: •...
  • Page 38 Using the HDR-DAD Solution Method settings in the HDR-DAD Solution • In the AdvancedSpectrum section, the user may chose the storage of All spectra or None from a dropdown list. The HDR-DAD solution supports full HDR spectra data (190 – 640 nm) There is no support for other spectra modes.
  • Page 39 Using the HDR-DAD Solution Method settings in the HDR-DAD Solution • Peakwidth The data rate is settable from 0.31 – 80 Hz Figure 16 HDR-DAD Peakwidth settings • Slit The slit width is settable to 1, 2, 4, or 8 nm If one or both detectors are G7117A or G4212B (module has a fixed slit), the slit N O T E automatically is set to 4 nm and cannot be adjusted.

  • Page 40: Status Display During A Run With The Hdr-Dad

    Using the HDR-DAD Solution Status Display During a run with the HDR-DAD Status Display During a run with the HDR-DAD Figure 18 Display during a HDR-DAD run For details on the general functions of the status display, see the manual Agilent 1200 N O T E Infinity Series Diode Array Detectors, Agilent 1290 Infinity II Diode Array Detector or the online help.
  • Page 41 Using the HDR-DAD Solution Status Display During a run with the HDR-DAD Click on the menu items in the context menu of the status dashboard offers the following HDR-DAD solution specific functions: • Identify Device lets both detectors blink • Balance triggers balance on both detectors •...

  • Page 42: Control User Interface Of The Hdr-Dad Solution

    For details on the general functions of the control user interface, see the manual Agilent N O T E 1200 Infinity Series Diode Array Detectors, Agilent 1290 Infinity II Diode Array Detector or the online help. The following HDR-DAD specific functions exist: •...

  • Page 43: Method And Run Control User Interface In Chemstation

    Using the HDR-DAD Solution Method and Run Control User Interface in ChemStation Method and Run Control User Interface in ChemStation The Method and Run Control user interface provides information on the status of the system. Run control Online plots Spectra Figure 21 HDR-DAD solution control screen in ChemStation With EZChrom, the functionality of the HDR-DAD solution is equal and the status...
  • Page 44 Using the HDR-DAD Solution Method and Run Control User Interface in ChemStation Code for the signals observed: • The HDR-DAD signals are available as DAD1A - H signal • The original signals of DAD are available as LNG1A - H signal (= long cell) •...

  • Page 45: Hdr-Dad Solution - Data Analysis

    Using the HDR-DAD Solution HDR-DAD Solution - Data Analysis HDR-DAD Solution - Data Analysis The HDR-DAD solution offers the following sections for Data Analysis: • Three separate signal groups for HDR, DAD (LNG = long cell), DAD (SHT = short cell) The user can observe 24 signals 3 (HDR, DAD , DAD ) x 8 signals (A-H)
  • Page 46 Using the HDR-DAD Solution HDR-DAD Solution - Data Analysis Table 3 Differential display of delay time in ChemStation and EZChrom ChemStation EZChrom Figure 25 EZChrom displays a short gap at the end of the HDR signal (red rectangle) Figure 24 ChemStation displays a short gap at the beginning of the HDR signal (red rectangle) HDR-DAD Solution...

  • Page 47: Frequently Asked Questions (Faqs)

    HDR-DAD Solution Frequently Asked Questions (FAQs) Frequently Asked Questions This chapter answers frequently asked questions. Agilent Technologies...

  • Page 48: Frequently Asked Questions

    Frequently Asked Questions (FAQs) Frequently Asked Questions Frequently Asked Questions 1 What can I do if I observe a leap in the HDR signal? Wrong delay calibrations cause leaps in the data signal (see Figure 26 page 48). Double check the delay calibration between the 2 detectors. Figure 26 Leaps in the HDR signal 2 How are the signals named?
  • Page 49 Frequently Asked Questions (FAQs) Frequently Asked Questions 5 How can I print spectra information in OpenLAB CDS ChemStation edition? To print the correct spectra information, adhere to the following procedure: a Open Signal Details... dialog (in ChemStation via Calibration > Signal Details...).
  • Page 50 Frequently Asked Questions (FAQs) Frequently Asked Questions HDR-DAD Solution...

  • Page 51: Application And Technical Notes For The Hdr-Dad Solution

    HDR-DAD Solution Application and Technical Notes for the HDR-DAD Solution Application and Technical Notes This chapter gives an overview on additional literature. Agilent Technologies...

  • Page 52: Application And Technical Notes

    Application and Technical Notes Description 5991-0115EN Single-run assay and impurity testing of fixed-dose combination drugs using the Agilent 1200 Infinity Series High Dynamic Range Diode Array Detector Solution 5991-1409EN Reliable and automatic integration of trace compounds using the Agilent 1200 Infinity Series High Dynamic Range Diode Array...
  • Page 53 Index Index specifications flow cell GLP features flow connections analog signal output HDR-DAD application notes noise and drift (ASTM) programmable slit width GLP features safety and maintenance calculation signal data rate delay volume wavelength accuracy HDR-DAD wavelength bunching checklist data analysis wavelength range delivery method settings...
  • Page 54 Array Detector Solution (HDR-DAD) The manual describes the following: • Introduction • Specifications • Installation • Using • Frequently Asked Questions • Additional Information © Agilent Technologies 2014-2016, 2017 Printed in Germany 03/2017 *G2199-90001* *G2199-90001* G2199-90001 Rev. B Agilent Technologies...

Table of Contents