Margin For Negative Absorbance - Agilent Technologies 1200 Infinity Series User Manual

5
Optimizing the Detector
Optimizing for Sensitivity, Selectivity, Linearity and Dispersion

Margin for Negative Absorbance

The detector adjusts its gain during balance such that the baseline may drift
slightly negative (about -100 mAU). In some special case, for example, when
gradient with absorbing solvents are used, the baseline may drift to more
negative values.
Only for such cases, increase the margin for negative absorbance to avoid
overflow of the analog-to-digital converter.
Agilent 1200 Infinity Series DAD User Manual 95