Miltenyi Biotec MultiMACS Cell24 Separator Plus User Manual page 65

7 APPLY SAMPLE AND WASH : Apply the suspension of magnetically labeled cells onto each column and
let it run through.
When exceeding a volume of 5 mL, load the sample in two steps onto the column. Tap
applying the first 5 mL. Exchange the deep well plate after movement of the magnet. Tap
with sample loading. Alternatively, a 88 mm deep well plate can be used.
8 Apply wash buffer onto each column and let the buffer run through. Ensure that the column reservoir is
empty before applying new wash buffer. Tap
9 TIP-TOUCH COLUMNS IN PLATE : Move the Tip-Touch Plate firmly back and forth once. Tap OK . The
combined flow-through from the cell suspension and the washes represent the unlabeled target cell
fraction.
10 REMOVE PLATE. PLACE COLUMNS AND NEW PLATE IN ELUTION STATION : Remove the deep well
plate from the Tip-Touch Plate. Insert a new deep well plate into the base of the MACS Elution Chamber to
collect the positive fraction from the columns.
The deep well plate can be inserted in two directions. To ensure the correct assignment of the samples, note
the orientation of the deep well plate to the columns.
11 Place lid I on the base of the MACS Elution Chamber.
12 Remove the MultiMACS Single-Column Adapter with the MACS Columns from the Column Holder and place
it on lid I. Close empty cavities with Column Blanks. Tap
13 APPLY ELUTION BUFFER : Apply elution buffer onto each column to elute the labeled cells. Proceed to
the next step immediately.
14 Tap OK to apply the elution impulse.
15 Tap the MultiMACS Single-Column Adapter with the MACS Columns to the MACS Elution Chamber to collect
drops from the column tips and ensure complete recovery of the positive fraction.
16 REMOVE PLATE AND COLUMNS. TOUCH OK TO END PROCESS : Remove the MultiMACS Single-
Column Adapter with the MACS Columns and the lid of the MACS Elution Chamber.
17 Tap OK and proceed to downstream applications with the labeled target cells in the deep well plate.
OK .
OK .
MOVE BACK after
OK to proceed
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