Collecting The Unlabeled Fraction; Tip-Touch - Miltenyi Biotec MultiMACS Cell24 Separator Plus User Manual

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3.5.3

Collecting the unlabeled fraction

1 For application of samples and buffer the MultiMACS Cell24 Magnet and the MultiMACS Column Holder

move down.

2 Apply buffer or sample depending on the process step.

Equilibrate columns just before the separation. Do not store equilibrated columns.

3.5.4

The Tip-Touch Plate is used for the removal of residual drops from the column tips. The plate can be moved

back and forth to deposit the last drop into the wells. This ensures reproducible recoveries and avoids cross-

contamination. The column tips must protrude 1 mm into the well.

1 MultiMACS Column Holder and MultiMACS Cell24 Magnet remain down for the Tip-Touch process.

2 When the instrument prompts you to perform a Tip-Touch, move the Tip-Touch Plate firmly back and forth

once. Droplets from the column tips fall into the plate.

Tip-Touch can be ignored if single-well deep well plates are used.

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