Possel; Possel_2 - Miltenyi Biotec MultiMACS Cell24 Separator Plus User Manual

11 REMOVE COLUMNS, PRESS OK TO END PROCESS : Remove the columns containing the labeled cells.
Tap
12 Proceed to downstream applications with the unlabeled cells from the flow through fraction.
5.2

POSSEL

1 Select POSSEL in the Start menu. The Process menu opens. Tap the Run button.
2 INSERT COLUMNS : Insert the Multi-24 Column Block into the MultiMACS Column Holder. Tap OK .
3 INSERT WASTE PLATE : Insert a deep well plate onto the Tip-Touch Plate. Tap OK .
The MACS Elution Station will generate a vacuum. The status LED blinks green or red during this process.
When the vacuum has been established, the status LED lights up constantly green.
4 APPLY EQUILIB. BUFFER, SAMPLE AND WASH : Apply degassed equilibration buffer onto each
column. Let the buffer run through.
5 Apply the suspension of magnetically labeled cells onto each column and let it run through.
6 Apply degassed wash buffer onto each column and let it run through. Ensure that the column reservoir is
empty before applying new wash buffer. Tap
7 TIP-TOUCH COLUMNS IN PLATE : Move the Tip-Touch Plate firmly back and forth once. Tap OK .
5
8 REMOVE PLATE. PLACE COLUMNS AND NEW PLATE IN ELUTION STATION : Remove the deep well
plate from the Tip-Touch Plate. Insert a new deep well plate into the base of the MACS Elution Chamber to
collect the positive fraction from the columns.
The deep well plate can be inserted in two directions. To ensure the correct assignment of the samples, note
the orientation of the deep well plate to the columns.
9 Place lid I on the base of the MACS Elution Chamber.
10 Remove the Multi-24 Column Block from the MultiMACS Column Holder and place it on lid I.
11 Tap OK to apply the elution impulse.
12 APPLY ELUTION BUFFER : Apply elution buffer onto each column to elute the labeled cells.
13 Let the elution buffer run through completely. Tap OK .
14 Tap the Multi-24 Column Block to the MACS Elution Chamber to collect drops from the column tips and
ensure complete recovery of the positive fraction.
15 REMOVE PLATE AND COLUMNS. TOUCH OK TO END PROCESS : Remove the Multi-24 Column Block
and the lid of the MACS Elution Chamber.
16 Tap OK and proceed to downstream applications with the labeled target cells in the deep well plate.
5.3

POSSEL_2

1 Select POSSEL2 in the Start menu. The Process menu opens. Tap the Run button.
2 INSERT COLUMNS : Insert the Multi-24 Column Block into the MultiMACS Column Holder. Tap OK .
3 INSERT WASTE PLATE : Insert a deep well plate onto the Tip-Touch Plate. Tap OK .
4 APPLY EQUILIBRATION BUFFER : Apply degassed equilibration buffer onto each column and let the
buffer run through. Tap
5 TIP-TOUCH COLUMNS IN PLATE : Move the Tip-Touch Plate firmly back and forth once. Tap OK .
6 INSERT PLATE TO COLLECT UNLABELED FRACTION : Remove the waste plate and insert a new deep
well plate onto the Tip-Touch Plate. Tap
60
OK .
OK .
OK .
OK .