Miltenyi Biotec CliniMACS Plus System Manuals

Manuals and User Guides for Miltenyi Biotec CliniMACS Plus System. We have 2 Miltenyi Biotec CliniMACS Plus System manuals available for free PDF download: User Manual

Miltenyi Biotec CliniMACS Plus System User Manual (168 pages)

Brand: Miltenyi Biotec | Category: Laboratory Equipment | Size: 9 MB

Table of Contents
4
General
10
Introduction
10
Service Information
11
The Clinimacs® Plus Instrument
12
Regulatory Information
12
Intended Purpose
12
Warnings and Precautions
13
Specifications
15
Guidance and Manufacturer's Declaration on Electromagnetic Compatibility
17
Components of the Clinimacs® Plus Instrument
20
Unpacking and Installation
22
General Set-Up Menus
26
Cleaning
28
Maintenance
28
Instrument Disposal
28
Glossary
29
Glossary of Terms
33
Customer Information
37
MACS® Magnetic Cell Separation
37
The Clinimacs® Plus System
37
Additional Materials Required
38
Equipment Required
39
General Warnings and Precautions
40
Labeling of Cells with Clinimacs® Reagents
43
High-Gradient Magnetic Cell Separation
43
Clinimacs® Plus Separation Strategies
44
Enrichment Strategy
45
Depletion Strategy
46
Four STEPS to Your Target Cells
49
STEP 1: Cell Preparation and Magnetic Labeling
51
Clinimacs® CD34 Reagent
57
General Information
57
Materials Required
58
Clinimacs® Plus CD34 System Components
58
Additional Materials
58
Preparative Steps
59
Preparation of the Clinimacs® PBS/EDTA Buffer
59
Labeling and Preparation of Bags
59
Preparation of Leukapheresis Product
60
Analysis
60
Transfer into Cell Preparation Bag
61
Dilution
62
Centrifugation
63
Volume Adjustment
63
Magnetic Labeling of Cells
65
Incubation with the Clinimacs® CD34 Reagent
65
Removal of Excess Reagent
66
Clinimacs® CD19 Reagent
71
General Information
71
Materials Required
72
Clinimacs® Plus CD19 System Components
72
Additional Materials
72
Preparative Steps
73
Preparation of the Clinimacs® PBS/EDTA Buffer
73
Labeling and Preparation of Bags
73
Preparation of Leukapheresis Product
74
Analysis
74
Transfer into Cell Preparation Bag
75
Dilution
76
Centrifugation
77
Volume Adjustment
77
Magnetic Labeling of the Cells
79
Incubation with the Clinimacs® CD19 Reagent
79
Removal of Excess Reagent
80
Clinimacs® Tcrα/Β-Biotin
83
General Information
83
Materials Required
84
Clinimacs® Plus Tcrα/Β-Biotin System Components
84
Additional Materials
84
Preparative Steps
85
Preparation of the Clinimacs® PBS/EDTA Buffer
85
Labeling and Preparation of Bags
85
Preparation of Leukapheresis Product
86
Analysis
86
Transfer into Cell Preparation Bag
87
Dilution
88
Centrifugation
89
Volume Adjustment
89
Labeling of the Cells with the Clinimacs® Tcrα/Β-Biotin
91
Incubation with the Clinimacs® Tcrα/Β-Biotin
91
Removal of Excess Conjugate
91
Magnetic Labeling of the Cells with the Clinimacs® Anti-Biotin Reagent
94
Incubation with the Clinimacs® Anti-Biotin Reagent
94
Removal of Excess Reagent
95
Cd34 Selection 1/2
98
Switch-On of the Clinimacs® Plus Instrument
98
Choice of Separation Program CD34 SELECTION 1/2
98
Depletion 2.1
100
Switch-On of the Clinimacs® Plus Instrument
100
Choice of Separation Program DEPLETION 2.1
100
Step 2
100
Sample Parameter Input
102
Depletion 3.1
105
Switch-On of the Clinimacs® Plus Instrument
105
Choice of Separation Program DEPLETION 3.1
105
Step 2
105
Sample Parameter Input
107
Clinimacs® Tubing Set and Clinimacs Tubing Set LS
110
Preparation for Tubing Set Installation
110
Attach Cell Collection Bag
110
Attach Priming Waste Bag and Insert Pre-Column
111
Insert Separation Column and Load Valve No. 5
113
Load Valves Nos. 1, 2, 3, and 4
114
Load Pump Tubing
115
Load Valves Nos. 7 and 8
116
Load Valves Nos. 7 and
116
Load Valves Nos. 6, 9, 10, and
116
Recheck All Tubing and Attachments
117
Seating of Valves
117
Attach Clinimacs® PBS/EDTA Buffer
118
Start Priming
119
Check During the Priming
119
Final Check of All Tubing and Attachments
119
Integrity Test
120
Connect Cell Preparation Bag
122
Final Check of the Liquid Sensor
122
Alternative Installation of Clinimacs® Tubing Sets
124
Preparation for Tubing Set Installation
125
Attach Non-Target Cell Bag, Reapplication Bag, and Insert Separation Column
132
Load Valves Nos. 1, 2, 3, 4, and 5
134
Load Pump Tubing
135
Load Valves Nos. 6, 7, 8, 9, and 10
136
Recheck All Tubing and Attachments
136
Seating of Valves
136
Attach Clinimacs® PBS/EDTA Buffer
137
Start Priming
138
Check During the Priming
138
Final Check of All Tubing and Attachments
138
Integrity Test
139
Connect Cell Preparation Bag
141
Final Check of the Liquid Sensor
142
Cd34 Selection 1/2
144
Separation Procedure
144
Disconnect Bags and Record Process Code
146
Unload Tubing Set and Shutdown
147
Analysis of Cells
147
Depletion 2.1
148
Separation Procedure
148
Disconnect Bags and Record Process Code
150
Unload Tubing Set and Shutdown
151
Analysis of Cells
151
Depletion 3.1
152
Separation Procedure
152
Disconnect Bags and Record Process Code
154
Unload Tubing Set and Shutdown
155
Analysis of Cells
155
Troubleshooting
156
Preparation of the Leukapheresis Product
156
Clinimacs® Plus Instrument and Clinimacs Tubing Sets
156
Automated Cell Separation
158
Cell Separation Performance
165

Miltenyi Biotec CliniMACS Plus System User Manual (49 pages)

Brand: Miltenyi Biotec | Category: Laboratory Equipment | Size: 1 MB

Table of Contents
7
Introduction
9
General Information
9
Clinimacs Plus Instrument Information
10
Glossary
11
Graphical Depiction
11
Glossary of Symbols
12
Safety Symbols
12
Symbols Used for Labeling Products
12
Glossary of Terms
15
Important Safety Information
17
Safety Instructions for the Clinimacs Plus Instrument
19
Usage and Installation
19
Electric Hazards
20
Strong Magnetic Field
22
Biological Hazards
22
Mechanical Hazard
22
Servicing and Transport
23
The Clinimacs Plus Instrument
25
Regulatory Information
25
Intended Purpose
25
Technical Data
26
Power Connection
27
Unpacking
28
Transport
29
Positioning
29
Cleaning and Disinfection
30
Maintenance
30
Disposal
31
Description
31
Installation
33
Connect and Switch on the Clinimacs Plus Instrument
33
Language Selection and Service Menu
34
General Set-Up Menus
34
The Clinimacs Plus System
37
Clinimacs Plus Instrument
37
Clinimacs Reagents and Biotin Conjugates
37
Clinimacs Plus Tubing Sets
37
Clinimacs PBS/EDTA Buffer
37
Additional Materials Required
37
Troubleshooting
39
Miltenyi Biotec Technical Support
39
Error Messages
39
Legal Notes
41
Limited Warranty
41
Trademarks
42
Appendix
43

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