Partial Loop Injections - Waters 2707 Operator's Manual

Partial loop injections

Partial loop injections yield no sample loss and, like partial loop needle overfill
injections, maximum accuracy. However, their reproducibility specification is
slightly lower. In the standard configuration, peak area RSD is < 1% for
injection volumes > 10 µL.
Recommendation:
partial loop mode, consider the effect on the chromatography. In general
for reverse phase chromatography, match the wash solvent mixture to
the initial gradient conditions.
This is the partial loop injection sequence:
1. The injection valve moves to the Inject position, and the sample needle
moves to the Transport position.
Syringe
1-18 Understanding Operating Principles
Because wash solvent injects with the sample in
Buffer tubing
Needle
Wash station Sample
INJECT
Sample loop
Pump
Column