Calibration - Leica Aperio AT2 DX User Manual

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Chapter 4: Scanning Slides

Or click the Auto Select Focus Points icon

density.

After the focus points are automatically placed, you may decide to manually add more, move focus points, or delete focus

points. For example, if a focus point has been placed in a clear area of the slide without tissue, you will want to delete it or

move it to an area that contains tissue.

After opening a slide in the slide view window, you can work with focus points.

If you want to

Add focus points

Move focus points

Delete focus points

Automatically focus all

focus points

Calibration

The scanner is calibrated and verified at the factory before shipment. No further manual calibration or verification is required

by the customer. However, we recommend annual preventive maintenance, performed by Leica Biosystems Technical

Services, to make sure the scanner is operating correctly.

Every time you scan, the scanner calculates adjustments for minor illumination differences to ensure that the scanner

performs consistently to scan high-quality images.

The scanner makes a calibration image of the white background at the location of the blue diamond (which must be

positioned at a location that does not appear to have tissue) and then uses the image to obtain a shading correction that

will be applied during the scanning process. Calibration then performs white-balancing. The final step of calibration is to

measure intensity across the entire calibration image, and then normalize the calibration image to a predefined value. Not

only does this provide uniformity for images made by a single scanner, but calibration also ensures images are similar in

intensity across multiple scanners.

To manually make a calibration image, you must be manually scanning a slide in the slide tray.

to tell the scanner to automatically place focus points at the default

Do this

To add a focus point, double-click on the macro image at the position at which you want to

add a focus point. Or, right-click on that position and select Add Focus Point Here from

the context menu.

Click a focus point to select it and drag it to a new position.

Click the focus point to select it and press the Delete key on your keyboard. Or, click the

focus point select it and right-click. Select Delete This Point from the context menu. (You

can also hold down the Control key and select multiple focus points before doing these

procedures.)

If you are manually scanning a slide on the stage, you can tell the scanner to automatically

focus the focus points before scanning. This allows you to test that all focus points can be

focused.

Click the Auto Focus All Focus Points icon

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