Manually Scanning A Slide - Leica Aperio AT2 DX User Manual

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Add slide setting
If you have previously saved scan parameters as a slide setting, you can select it from the Slide
Settings drop-down list to apply the settings to selected slides.
Select slide type
To assist you with scanning certain types of slides (such as faintly stained slides), scan parameters
have been preset for those types of slides. You can apply a slide type to the selected slides by
selecting one from the Slide Type drop-down list.

Manually Scanning a Slide

Certain features of the Console are only available when manually scanning the slide in the slide tray rather scanning slides
in a rack. This is because the slide is physically under the scanner objective while you are reviewing it.
Features only available when manually scanning a slide include:
Creating a calibration image (see "Calibration" on page 30).
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Auto focusing focus points before the scan (see "Working with Focus Points" on page 29).
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To place the slide on the scanner stage:
1.
First, eject the slide tray by clicking the Eject Stage button
manual scan even if the stage is already ejected (for example, after homing the stage).
2.
Place the slide on the slide tray with coverslip up and the label to the front. The Manual Scan window appears
where you can set name and description for the slide, select a slide type, and a magnification.
Although you could just click the Scan button on the Manual Scan window to start scanning the slide, you will probably
want to take a snapshot to get a macro image of the slide by clicking the Take Snapshot button to see the slide view
window, shown below.
For details on the icons in the Manual Setup section shown on the slide view window above, see the following sections of
this chapter.
Aperio AT2 DX User's Guide, Revision B © Leica Biosystems Imaging, Inc. 2020
at the bottom of the window. Do this to initiate a
Chapter 4: Scanning Slides
27

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