Using Snapshots - Leica Aperio AT2 DX User Manual

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Chapter 4: Scanning Slides

Using Snapshots

The scanner provides an automated workflow that is optimized to provide the best scanning experience in most cases.

Many operators find it easiest to load the slides into the racks and click the Console Scan button, letting the scanner

automatically find the tissue on the slide, set focus points, and scan. In most cases, this default operation provides excellent

results. In some cases, however, workflow is improved if the operator first views a quick snapshot of the slides, manually

sets the scan areas, sets focus points, chooses slide type, and so on, before scanning.

To take snapshots, select one or more slides in rack view or batch view and click the Snapshot button:

After snapshots have been made, select slides in rack view or batch view that display the light blue status color. While your

cursor is over one of the selected slides, click the View Slide icon.

You see the slide view window (shown above).

In the slide view window, click the forward arrow or back arrow to move between snapshots. Use the zoom slider to zoom

into the image—click the blue dot to zoom into the entire macro image, the red dot to zoom into the maximum scan area

(the red box), and the green dot to zoom into the scan area (the green box).

If you want to

Set the scan area

Verify the calibration

point position

Set focus points

Scan the slide

View the scanned

image

Do this

The area of the slide that will be scanned is marked by a green box (this is limited by the red

box, the maximum scan area).

To reset the scan area by letting the scanner detect the tissue area on the slide, click the Auto

select tissue icon:

Or, click the green pin at the top left of the scan area box to redraw the scan area. Or, click a

handle on the scan area box to drag the edges or corners of the box to a new position.

Make sure the calibration point (the blue diamond) is on a clear area of the slide that does

not contain tissue and that is under the coverslip. Click on it and drag it to a new position if

necessary.

You can add, delete, or move focus points. See "Working with Focus Points" below.

Click the Scan button to scan this slide and all slides that are ready for scanning.

If the image has already been scanned, a small magnifying glass appears at the top left of the

image. Click it to open the scanned image in Aperio ImageScope DX.

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