Loading Samples; Using The 96-Position Sample Tray With Microamp Reaction Tubes - Applied Biosystems GeneAmp PCR System 9600 User Manual

Loading Samples

The GeneAmp PCR System 9600 uses a 96-position sample tray
(MicroAmp Tray) and retainer (MicroAmp Retainer) for 0.2-mL
MicroAmp Reaction Tubes.

Using the 96-Position Sample Tray with MicroAmp Reaction Tubes

To load the 96-position MicroAmp Tray, follow the steps below
(refer to Figure 6-1).
CAUTION
oil-free operation. It does not use mineral oil or glycerine in the
sample block. The MicroAmp Reaction Tubes fit tightly in the wells
and a heated cover exerts an even pressure on all tubes. No
mineral oil is used on the sample surface because the heated
cover eliminates condensation on the tubes.
1
2
3
Note
or more samples. If you are only loading a few samples, use the sample
tray base as a working tray in which to load your sample tubes, then
transfer your tubes to a sample tray already loaded in the instrument.
Do not use the MicroAmp Retainer if you want to remove a single tube
without removing the caps from all the tubes. The caps will not fit
through the retainer.
6-2
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The GeneAmp PCR System 9600 is designed for
Place the MicroAmp Base on a flat surface such as a table. Do not
place the base in the sample block.
Place the MicroAmp Tray onto the base so that the well numbered
A1 is located at the upper left corner of the tray. The cutout in the
outside rim of the tray will then be located on the left side of the tray.
This orients the tray for the proper fit into the sample block.
Place MicroAmp Reaction Tubes in the tray. Note that the wells in
the tray are labeled left to right from 1 through 12 and A through H
from back to front.
The above procedure is most useful if you are loading a dozen