Wavelength Verification And Calibration - Agilent Technologies 1260 Infinity G1315C User Manual

8
Test Functions

Wavelength Verification and Calibration

Wavelength Verification and Calibration

The detector uses the alpha (656.1 nm) and beta (486 nm) emission lines of
the deuterium lamp for wavelength calibration. The sharp emission lines
enable more accurate calibration than is possible with holmium oxide. When
verification is started, the 1-nm slit is moved into the light path automatically,
and the gain is set to zero. To eliminate effects due to absorbing solvents, the
test should be done with bubble free degassed HPLC water in the flow cell.
If a deviation is found and displayed, it can be recalibrated by pressing Adjust.
The deviations are tracked in the Calibration History (diagnosis buffer in the
detector).
Figure 57
160
Wavelength Verification and Calibration
Agilent 1260 Infinity DAD and MWD User Manual