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Agilent Technologies G1982-6700 Upgrade Installation Manual

Hplc-chip/nanospray upgrade kit
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HPLC-Chip/Nanospray
Upgrade Installation
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Summary of Contents for Agilent Technologies G1982-6700

  • Page 1 HPLC-Chip/Nanospray Upgrade Installation Guide...
  • Page 2 No part of this manual may be reproduced in any form or by any means (including electronic storage and retrieval or translation into a foreign language) without prior agreement and written consent from Agilent Technologies, Inc. as governed by United States and international copyright laws.

  • Page 3: Table Of Contents

    Contents Introduction Kit Contents Required Tools Prepare for the upgrade Installation Upgrade the HPLC-Chip Interface Replace the gas distributor inside the desolvation assembly New Default Method Set Points and Reference Mass Introduction Procedure New Reference Mass Introduction Procedure Preparation of the IRM solutions Low Mass IRM stock solution: 1000 µg/ml methyl stearate in ACN IRM Working Solution: 100 µg/ml methyl stearate and 2% (v/v) HP-1221 in ACN Use of the IRM Solutions...

  • Page 4: Introduction

    Introduction This document describes the installation of the HPLC-Chip/Nanospray Upgrade Kit. This upgrade is designed for all Agilent LC/MS instruments utilizing the HPLC-Chip interface in either the HPLC-Chip configuration or Nanospray configuration. This document and procedure assumes that air is present in the drying gas supply. If no air is available, the HPLC Chips will charge which appears as unstable spray on the video capture device or black/white monitor.
  • Page 5 G1982-67000 HPLC-Chip/Nanospray Upgrade Kit Cont. Quantity Part Part Number Part Description Picture G1982-20008 Holey Counter Electrode G1982-20011 Wing Nut, Stainless Steel G1982-20111 End Cap Nano Spray Shield G1982-20012 Wave washer, Gold plated G1982-60004 Cover with flex steel GT430-20358 Electrode Spacing Tool 59987-20040 Dielectric Capillary 0515-1644...

  • Page 6: Required Tools

    G1982-67000 HPLC-Chip/Nanospray Upgrade Kit Cont. Quantity Part Part Number Part Description Picture 0100-2437 PEEK Nut 0100-2611 ZDV Union 5065-9906 LC-MS Capillary Connector G1982-85001 Chip Cube High Mass Reference (HP-1221) Shown G1982-85002 Chip Cube High Mass Solvent (FC-70) Shown G1982-85003 Chip Cube Low Mass Reference Sample Shown Required Tools The following tools are required for the installation of the HPLC-Chip/Nanospray Upgrade Kit.

  • Page 7: Prepare For The Upgrade

    Prepare for the upgrade 1. If installed, remove the HPLC-Chip Cube and HPLC-Chip Cube interface from the LC/MS instrument. 2. Remove the HPLC-Chip interface spray shield and gas diverter. Properly dispose these parts as they will no longer be used and are not compatible with the new gas diverter. 3.

  • Page 8: Installation

    2 Installation This section describes the installation of the HPLC-Chip/Nanospray Upgrade Kit hardware. Upgrade the HPLC-Chip Interface 1. While the LC/MS instrument is venting, remove and properly dispose of the following parts from the HPLC-Chip interface: • Counter Electrode – Use the supplied Hex Wrench (part number 8710-2623) to remove the counter electrode.

  • Page 9: Replace The Gas Distributor Inside The Desolvation Assembly

    2. Locate the new Holey Counter Electrode (part number G1982-20008). 3. Using the hex wrench, install the new counter electrode. Set the Holey Counter Electrode flush against the Electrode Mounting Bracket. Temporarily tighten the set screw to keep the new counter electrode in place. The final spacing adjustment will be performed with the interface installed on the instrument.
  • Page 10 Discard the existing endplate. Save the UIRM Union for use with other sources. 2. Remove the Chamber and Capillary High Voltage cables from the T-nut connectors. 3. Remove the two existing high voltage T-nut connectors that are used to secure the end plate and discard.
  • Page 11 6. Remove the Teflon endplate insulator block to access the vespel gas distributor. Remove and discard the old gas distributor. 7. Locate the new dielectric capillary (part number 59987-20040). Dip or wet the capillary with Isopropanol before installing to lubricate it. Install the new capillary. Do not twist the capillary while installing it.
  • Page 12 8. Locate the new gas distributor (part number G1964-20303). With the solid side facing the Teflon wall, slide the new gas distributor over the new capillary. Push it on until it is flush with the Teflon block. G1964-20303 Gas Distributor 9.
  • Page 13 Do not over-tighten these screws! 13. Reinstall the standard spray shield and capillary cap. When installing the capillary cap, do not twist or the capillary can be permanently damaged. 14. Plug the instrument back into the wall outlet and begin the pump down procedure. 15.
  • Page 14 Assemble the new spray shield and gas diverter assembly 1. While the instrument is tuning or pumping down, locate the following parts: Winged Nut (part number G1982-20011) • End Cap Nano Spray (G1982-20111) • Radial Gas Diverter (part number G1982-20006) •...
  • Page 15 4. Thread the vespel Radial Gas Diverter into Winged Nut (part number G1982-20011). Make sure to thread the Radial Gas Diverter completely into Winged Nut. 5. Once all of the parts have been assembled, rinse all of these parts with Isopropanol in a clean, glass beaker at least three times.
  • Page 16 8. Allow the instrument to complete the High Voltage Conditioning and Check Tune process before proceeding. Once the tune process has completed, convert the system back to Chip Cube mode. 9. Remove the currently installed spray shield and capillary cap. Store them in a clean, lint free bag.
  • Page 17 1. Install the HPLC Chip Interface onto the desolvation assembly. Please note that the Top Plate should not be installed at this time. 2. Connect the power cables for the illumination LED and video cable for the camera. 3. Locate the Electrode Spacing Tool (part number GT430-20358). 4.
  • Page 18 6. Once the spacing is set correctly, tighten the set screw that secures the Holey Counter Electrode in place. Once the set screw has been tightened, recheck the spacing to ensure that there is no extra space. Otherwise, the high voltage field will not be correct. Realign the Illumination LED and Camera Because the Holey Counter electrode has different depth dimensions, the illumination LED will not be pointing at the right location.
  • Page 19 This is the actual illumination LED spot showing the desired alignment. 6. Once the best position has been achieved, tighten the set (grub) screw. 7. If the camera power cable and video cable have been disconnected, reconnect those cables. 8. Reinstall the HPLC Chip Cube Interface on the LC/MS instrument. 9.
  • Page 20 11. With the top plate installed, examine the image on the video monitor. If the image is not clear or appears blurry, the camera is not focused correctly. 12. To refocus the camera, using the supplied 1.5 mm Allen Wrench (part number 8710-2623) loosen the set (grub) screw that secures the camera in place.
  • Page 21 Page 21 of 33 18 July 2011...

  • Page 22: New Default Method Set Points And Reference Mass Introduction Procedure

    3 New Starting Set Points and Default Method Set Points Before the upgraded HPLC-Chip Interface can be used, the drying gas flow, temperature, and capillary voltages must be adjusted from previous values. The drying gas flow behavior within the interface is significantly different from the previous design. The heat is more evenly distributed while the gas turbulence is significantly lower.
  • Page 23 • 290°C for MassHunter Acquisition for TOF and Q-TOF B.05.00 or higher (still under development and may change with final release). • 150°C for MassHunter Acquisition for QQQ B.04.01 or higher. The default Drying Gas Flow for all applications shall be: •...

  • Page 24: New Reference Mass Introduction Procedure

    4 New Reference Mass Introduction Procedure Reference Mass Solution Operation with a new Top Plate – For Use with TOF or Q-TOF LC/MS Instruments ONLY! PLEASE NOTE: These instructions apply only to 6200 Series TOF and 6500 Series Q-TOF LC/MS systems with an HPLC-Chip/MS interface. Do not use the reference mass solution with 6400 Series Triple Quad or 6300 Series Ion Trap LC/MS Systems.

  • Page 25: Low Mass Irm Stock Solution: 1000 Μg/Ml Methyl Stearate In Acn

    70) is applied. While the use of FC-70 is optional, and signals from the IRM compounds will be obtained without its use, the response is more uniform and lasts longer when it is used. Note that ACN and FC-70 are immiscible and so should not be mixed together before applying them to the wick.
  • Page 26 • Allow about five minutes for the ACN to evaporate at a typical standby source temperature of 300 °C. This pause is important to minimize the chances of arcing when the instrument is turned on. • Load a pipetter with the G1982-85002 FC-70 liquid (e.g., 200 µL), and add it to the wick in the same manner as for the IRM working solution.
  • Page 27 You will not see any signals from the IRM compounds unless there is a spray present and the mobile phase contains electrolytes. The IRM signals will be unstable during the startup and stabilization of the spray, which may take ten minutes or longer at a drying gas temperature of 350 °C and flow of 4 L/min.

  • Page 28: Troubleshooting Tips

    2007), it is recommended to keep the abundances below the value of 90 * (number of transients per scan) to avoid saturation effects. For 2 scans/second (4,880 transients/scan), the desirable range is between ca. 5,000—440,000 counts abundance. The ion signals from the IRM compounds decay quickly at first but should stabilize at a lower usable level for days.
  • Page 29 and this is the cause of the unstable spray. Otherwise, the flow from the nanopump may be unstable. The spray will fluctuate if there is a leak in a connection or a bubble in the flow path from the nanopump. Make sure the connections are leak-free, or purge the nanopump. The air addition to the drying gas may not be at the optimum flow rate.
  • Page 30 temporarily set the temperature to 365 °C and drying gas flow to 10 L/min, and bake out the interface assembly for a few hours or overnight. When I plot a TIC or BPC, all I see is a high baseline offset from the IRM compounds and little peaks or no peaks from my sample.

  • Page 31: Calibration Or Auto Tuning Using Cds And Infusion Chip

    5 Calibration or Auto Tuning Using CDS and Infusion Chip This section was left blank intentionally. Page 31 of 33 18 July 2011...

  • Page 32: Static Needle Holder Installation And Use

    6 Static Needle Holder Installation and Use With the upgraded HPLC-Chip Interface, a second top plate has been included to provide users an alternative to the HPLC Chip Cube Interface so that “classic” Nanospray emitters can be used. This top plate includes a universal needle holder, top plate, and optimized grounding path for the needle holder.
  • Page 33 In This Book The manual describes the following: • Installation of the HPLC Chip Cube Spray chamber upgrade hardware. • New instructions for IRM Solution © Agilent Technologies 2011 Printed in USA Revision A Page 33 of 33 18 July 2011...

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