Chapter 4: Preparing For Operation; Determining The Rotor Speed - Beckman Coulter JCF-Z Instructions For Use Manual

Zonal and continuous flow rotor
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Set system variables according to the parameters presented in this section.

Determining the Rotor Speed

The maximum allowable run speed for the JCF-Z rotor is 20 000 rpm, as long as the mean liquid
density in the rotor is 1.45 g/mL or less. If the mean solution density in the rotor is greater than 1.45
g/mL, the maximum allowable rotor speed must be reduced according to equation (1):
Equation (1)
RPM
=
20 000
Whether particles of a certain size (and all larger particles) are retained in the rotor or not depends
on a combination of flow rate and rotor speed. The speed determined with equation (1) is the
maximum speed at which the rotor may be run, and should not be exceeded. In addition, the rotor
should not be used at speeds slower than 2000 rpm (the minimum speed required for adequate
vertical positioning of the rotor contents).
PN JCFZ-IM-12AB
1.45 g/mL
--------------------------------------------- -
density of contents
Preparing for Operation
CHAPTER 4
4-1

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