A Principles Of Good Microbiology Techniques - Kendro HERAcell 240 Operating Instructions Manual

Attachment A: Principles of Good Microbiology Techniques
General Rules:
•
All doors and windows in the work area should be
closed while work is in progress.
•
Food and beverages are not permitted in the work
area.
No food should be stored in the work area.
•
Lab coats or other protective clothing must be
worn in the work area.
•
Pipetting by mouth is prohibited. Pipetting aids
must be used.
•
Syringes and needles may only be used if abso-
lutely necessary.
•
The formation of aerosols must be avoided during
any manipulations.
•
At the conclusion of work and prior to leaving the
work area, personnel must wash their hands and,
if necessary, disinfect, and moisturize them.
•
The work area should be kept clean and tidy. Only
equipment and materials actually required should
be stored on lab benches. Supplies should be sto-
red in the areas or cabinets provided.
•
If required for an assessment of the hazard risk,
the identity of the employed biological agents
must be regularly checked. Inspection intervals
depend on the degree of potential hazard.
•
When working with biological agents, all person-
nel are to be trained regarding safety at the work-
place prior to starting their activities, and at least
annually thereafter.
•
Any individuals unfamiliar with microbiology, viro-
logy, or cellular biology must receive special, in-
depth instructions and must be carefully supervi-
sed.
•
If necessary, regular pest control measures must
be carried out.
HERAcell
The following additional basic rules apply when
working with pathogens:
•
All work areas are to be disinfected daily. If ne-
cessary, disinfectants are to be changed regularly
to prevent a buildup of resistant germs.
•
Protective clothing may not be worn outside the
work area.
•
Contaminated equipment must be autoclaved or
disinfected before being cleaned.
•
Waste containing pathogenic material must be
safely collected and be autoclaved or disinfected
to make it inactive.
•
If infectious material is spilled, the contaminated
area must be immediately sealed off and disin-
fected.
•
When working with human pathogens against
which vaccines exist, all personnel that are not al-
ready immune to the pathogen in question must
be vaccinated. An appropriate method must be in
place to regularly monitor the immunity of the per-
sonnel.
•
The health of all personnel is to be monitored by
appropriate medical procedures. In other words, a
preliminary examination prior to starting work, fol-
lowed by annual examinations. FRG: With respect
to these examinations, professional regulations G
24 "Skin Diseases" and G 42 "Infectious Disea-
ses", are available to physicians. These are reco-
gnized principles of occupational medicine and
serve as guidelines for the evaluation, assess-
ment, and acquisition of examination results.
•
Professional guidelines are to be observed when
handling genetically altered organisms, viruses,
and subviral agents with a potential hazard risk,
FRG: G 43 "Biotechnology".
•
Information concerning first aid in cases of acci-
dental exposure to pathogenic microorganisms
and viruses must be immediately available in the
work area. All accidents must be immediately re-
ported to the responsible supervisor.
1
To be appropriately applied to cell cultures.
- 66 -
®
240 CO Incubator Operating Instructions
2
1
Version: 03.2001 / 50065640