Operation; Preparing The Unit; Beginning Operation - Kendro HERAcell 240 Operating Instructions Manual

®
HERAcell 240 CO Incubator Operating Instructions
2
8.

Operation

8.1

Preparing the Unit

The unit may only be released for actual operation if
all the measures for initial setup (Sec. 6.1 – 6.7) have
been properly carried out.
Before beginning operation, a unit check must be
performed to determine the condition of the following
components:
•
The gas hose must be mounted on the connecting
filter, must not leak, and must be secured with the
hose clamp.
•
The access port must be plugged.
•
The pressure compensation opening must be
open.
•
The glass door seal must not be damaged.
•
The measurement opening in the glass door must
be plugged.
•
The rack system components must be securely
installed.
NOTE:
The attachments to these operating instructi-
ons include a section, "Principles of Good Microbiolo-
gical Techniques" as safety information for individuals
who work with this unit.
NOTE – Water supply:
The water reservoir in the sample chamber
can hold a maximum of 4.5 l of treated water. For
continuous operation, an adequate supply of treated
water which meets both of the following criteria is re-
quired for humidifying:
•
Distilled;
•
Autoclaved, sterile.
The water reservoir must be topped up when the level
drops below the minimum mark.
Version: 03.2001 / 50065640
8.2

Beginning Operation

Starting and loading the unit:
1. Add a maximum of 4.5 l of treated water to the
water reservoir in the sample chamber. Do not
exceed the maximum level indicated by the upper
fill mark.
2. Make sure the CO
3. Switch the unit on at the main power switch.
4. Define the temperature and CO
at the control panel.
5. Open both unit doors until the audible alarm so-
unds.
6. Start the unit with the auto-start routine (refer to
Section 7.7).
7. Close the unit doors.
8. The temperature regulator adjusts to the tempe-
rature setpoint and the humidity builds up.
9. Once temperature and humidity have reached a
constant value, the CO
automatically equalized.
10. The "auto-start" indicator goes out.
11. The CO regulator supplies gas until the specified
2
CO setpoint is reached.
2
12. The unit is now operational.
13. Place the cultures in the sample chamber.
NOTE – Duration of the auto-start routine:
As a rule, the routine requires 5 to 7 hours.
Where the ambient temperature is low or the unit is
cold, the routine may take up to 10 hours.
NOTE – Loading:
To provide adequate air circulation and uni-
form heating of the samples, the available area in the
sample chamber should only be filled to approx. 70 %.
Objects with large surface areas or equipment which
generates heat in the sample chamber can inhibit heat
distribution.
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supply system valve is open.
2
content setpoints
2
measurement system is
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